Multienzyme Assemblies and Dynamics in Acetogenesis and Methanogenesis PDF Download
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Author: Steven E. Cohen (Ph. D.)
Publisher:
ISBN:
Category :
Languages : en
Pages : 148
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Book Description
The Wood-Ljungdahl pathway of acetogenesis allows for growth utilizing carbon dioxide as the sole carbon source. This process uses a series of metalloenzymes to catalyze the reduction of two molecules of carbon dioxide to acetyl-CoA. Key to this pathway are the bifunctional carbon monoxide dehydrogenase/acetyl-CoA synthase (CODH/ACS) and corrinoid iron-sulfur protein (CFeSP). CODH/ACS is a bifunctional enzyme, with the CODH subunit catalyzing the reduction of carbon dioxide to carbon monoxide. CFeSP is a cobalt-containing corrinoid-dependent methyltransferase. The active site of ACS, termed the A-cluster, is a nickel, iron, and sulfur-containing metallocluster which catalyzes the synthesis of acetyl-CoA from carbon monoxide provided by CODH, a methyl group provided by CFeSP, and CoA. Despite the unprecedented organometallic chemistry performed at the A-cluster much is unknown about this ACS activity. No substrate-bound structures of ACS have ever been reported and the molecular basis for ACS and CFeSP interactions are largely uncharacterized. Here we present the structure of the carbonylated A-cluster in CODH/ACS, highlighting the role of conformational dynamics in catalysis. We further characterize ACS dynamics using negative-stain electron microscopy, demonstrating a much larger extent of conformational flexibility than has been crystallographically observed. The basis for CFeSP:ACS interactions is further interrogated using a series of CFeSP domain constructs, showing the corrinoidbinding Rossmann fold to be sufficient for ACS interactions. Finally, we report preliminary structural characterization of acetyl-CoA decarbonylase/synthase (ACDS), a 2.2 MDa complex of CODH, ACS, and CFeSP found in archaeal methanogens. Together, this advances our understanding of the roles of structure and dynamics in the Wood-Ljungdahl pathway.
Author: Steven E. Cohen (Ph. D.)
Publisher:
ISBN:
Category :
Languages : en
Pages : 148
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Book Description
The Wood-Ljungdahl pathway of acetogenesis allows for growth utilizing carbon dioxide as the sole carbon source. This process uses a series of metalloenzymes to catalyze the reduction of two molecules of carbon dioxide to acetyl-CoA. Key to this pathway are the bifunctional carbon monoxide dehydrogenase/acetyl-CoA synthase (CODH/ACS) and corrinoid iron-sulfur protein (CFeSP). CODH/ACS is a bifunctional enzyme, with the CODH subunit catalyzing the reduction of carbon dioxide to carbon monoxide. CFeSP is a cobalt-containing corrinoid-dependent methyltransferase. The active site of ACS, termed the A-cluster, is a nickel, iron, and sulfur-containing metallocluster which catalyzes the synthesis of acetyl-CoA from carbon monoxide provided by CODH, a methyl group provided by CFeSP, and CoA. Despite the unprecedented organometallic chemistry performed at the A-cluster much is unknown about this ACS activity. No substrate-bound structures of ACS have ever been reported and the molecular basis for ACS and CFeSP interactions are largely uncharacterized. Here we present the structure of the carbonylated A-cluster in CODH/ACS, highlighting the role of conformational dynamics in catalysis. We further characterize ACS dynamics using negative-stain electron microscopy, demonstrating a much larger extent of conformational flexibility than has been crystallographically observed. The basis for CFeSP:ACS interactions is further interrogated using a series of CFeSP domain constructs, showing the corrinoidbinding Rossmann fold to be sufficient for ACS interactions. Finally, we report preliminary structural characterization of acetyl-CoA decarbonylase/synthase (ACDS), a 2.2 MDa complex of CODH, ACS, and CFeSP found in archaeal methanogens. Together, this advances our understanding of the roles of structure and dynamics in the Wood-Ljungdahl pathway.
Author: Edward F. DeLong
Publisher: Springer
ISBN: 9783642301193
Category : Science
Languages : en
Pages : 567
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Book Description
The Prokaryotes is a comprehensive, multi-authored, peer reviewed reference work on Bacteria and Achaea. This fourth edition of The Prokaryotes is organized to cover all taxonomic diversity, using the family level to delineate chapters. Different from other resources, this new Springer product includes not only taxonomy, but also prokaryotic biology and technology of taxa in a broad context. Technological aspects highlight the usefulness of prokaryotes in processes and products, including biocontrol agents and as genetics tools. The content of the expanded fourth edition is divided into two parts: Part 1 contains review chapters dealing with the most important general concepts in molecular, applied and general prokaryote biology; Part 2 describes the known properties of specific taxonomic groups. Two completely new sections have been added to Part 1: bacterial communities and human bacteriology. The bacterial communities section reflects the growing realization that studies on pure cultures of bacteria have led to an incomplete picture of the microbial world for two fundamental reasons: the vast majority of bacteria in soil, water and associated with biological tissues are currently not culturable, and that an understanding of microbial ecology requires knowledge on how different bacterial species interact with each other in their natural environment. The new section on human microbiology deals with bacteria associated with healthy humans and bacterial pathogenesis. Each of the major human diseases caused by bacteria is reviewed, from identifying the pathogens by classical clinical and non-culturing techniques to the biochemical mechanisms of the disease process. The 4th edition of The Prokaryotes is the most complete resource on the biology of prokaryotes. The following volumes are published consecutively within the 4th Edition: Prokaryotic Biology and Symbiotic Associations Prokaryotic Communities and Ecophysiology Prokaryotic Physiology and Biochemistry Applied Bacteriology and Biotechnology Human Microbiology Actinobacteria Firmicutes Alphaproteobacteria and Betaproteobacteria Gammaproteobacteria Deltaproteobacteria and Epsilonproteobacteria Other Major Lineages of Bacteria and the Archaea
Author: H.W. Verseveld
Publisher: Springer Science & Business Media
ISBN: 9400935390
Category : Medical
Languages : en
Pages : 300
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Book Description
The 5th International Symposium on Microbial Growth on C Compounds was held at the Biological 1 Center of the University of Groningen, Haren, The Netherlands, 11-16 August 1986. The meeting attracted well over 200 participants from 15 countries. This volume contains the formal presentations made at that time, which, because of the breadth of topics covered, were divided into seven sections of related papers. This meeting, under the chairmanship of Wim Harder, was both scientifically and socially very successful. This success cannot only be credited to the main presentations, but also to the well cared for 121 poster presentations, whereof the abstracts have been published separately. The series of Symposia will be continued in 1989, in the Federal Republic of Germany. We wish to acknowledge the invaluable help of Joke Daniels, Roberta Stroer-Schneider, Karin Uyldert, Hansje Bartelson and Josine van Verseveld-Stroer, who retyped the manuscripts resulting in a uniform presentation of these proceedings.
Author: Mansour Al Qubeissi
Publisher: BoD – Books on Demand
ISBN: 1789855357
Category : Science
Languages : en
Pages : 189
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Book Description
Amongst concerns about climate change, energy security decline and depletion of fossil fuels, this book explores the high importance of and interests in alternative energy resources. Many studies have shown that biomass fuels are sustainable, environmentally friendly and can be the most appropriate replacement to the depleting crude oil. Additionally, they can expand green landscapes, create new job opportunities, be directly utilised in standard power systems and improve combustion performance. Biomass fuels can be limited due to production cost and competition with food. Therefore, plant and food wastes play an important role in reducing these costs and recycling dump bio-materials. Production of biofuels from non-food biomass has emerged as a sustainable option to tackle the problems associated with growing demands for energy.
Author: Nigel P. Minton
Publisher: Springer Science & Business Media
ISBN: 1475797184
Category : Medical
Languages : en
Pages : 313
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Book Description
To the uninitiated, the genus Clostridium is likely more to be associated with disease than biotechnology. In this volume, we have sought to remedy this misconception by compiling aseries of chapters which, together, provide a practically-oriented handbook of the biotechnologie potential of the genus. Clostridium is a broad grouping of organisms that together undertake a myriad of biocatalytic reactions. In the first two chapters, the reader is introduced to this diversity, both taxonomically and physiologically. In the following chapter, the current state of genetic analysis of members of the genus is reviewed. The remaining chapters concentrate on specific, exploit able aspects of individual Clostridium species-highlighting their range of unique capabilities (of potential or recognized industrial value), particu larly in the areas of biotransformation, enzymology, and the production of chemical fuels. Fittingly, the final chapter demonstrates that even the most toxic of the clostridia can be of therapeutic value. The contributors to this volume reflect the trans national interest in Clostridium, and we are indebted to each of them for making this volume possible. We particularly wish to acknowledge the contributions, both to this volume and to microbiology in general, of Dr. Elizabeth Cato, who, sadly, died shortly be fore publication ofthis volume. Finally, we would like to join the authors in recommending closer and wider consideration of the attributes and capabilities of this genus.
Author: Moselio Schaechter
Publisher: Academic Press
ISBN: 0080961282
Category : Science
Languages : en
Pages : 1277
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Book Description
The Desk Encyclopedia of Microbiology, Second Edition is a single-volume comprehensive guide to microbiology for the advanced reader. Derived from the six volume e-only Encyclopedia of Microbiology, Third Edition, it bridges the gap between introductory texts and specialized reviews. Covering topics ranging from the basic science of microbiology to the current "hot" topics in the field, it will be invaluable for obtaining background information on a broad range of microbiological topics, preparing lectures and preparing grant applications and reports. The most comprehensive single-volume source providing an overview of microbiology to non-specialists Bridges the gap between introductory texts and specialized reviews Provides concise and general overviews of important topics within the field making it a helpful resource when preparing for lectures, writing reports, or drafting grant applications
Author: Rod Greenshields
Publisher:
ISBN:
Category : Science
Languages : en
Pages : 464
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Book Description
This volume consists of major articles from the journal "Industrial Biotechnology" which relect both the educational nature of much of the journal's output and the very wide range of its subject matter. They consider major applications in pharmaceuticals and other areas.
Author: Max M. Häggblom
Publisher: Springer Science & Business Media
ISBN: 0306480115
Category : Science
Languages : en
Pages : 702
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Book Description
Halogenated organic compounds constitute one of the largest groups of environmental chemicals. The industrial production of new halogenated organic compounds has increased throughout the last century peaking in the 1960s, and continuing in widespread use today. Organohalides are integral to a variety of industrial applications, including use as solvents, degreasing agents, biocides, pharmaceuticals, plasticizers, hydraulic and heat transfer fluids, and intermediates for chemical synthesis, to name a few. It is important to recognize the beneficial aspects of halogenated organic compounds, as well as their potentially deleterious impact on the environment and health. Recognition ofthe adverse environmental effects ofmanytypes oforganohalide compounds has led to efforts to reduce or eliminate the most problematic ones. Although organohalide compounds are typically considered to be anthropogenic industrial compounds, they have their counterpart in several thousands of natural biogenic and geogenic organohalides, representing most classes of organic chemicals. Natural sources account for a significant portion of the global organohalogen budget. This volume authored by recognized experts in the field provides a current perspective on how both natural and synthetic organohalides are formed and degraded, and how these processes are incorporated into a global halogen cycle. The focus is on microbial processes, since these play a major role both in the production and degradation, i. e. , cycling of halogenated organic compounds inthe environment. This book is organized into five parts. Part I, Introduction, provides a global perspective on the issues of organohalides and their fate in the environment.
Author: Inamuddin
Publisher: Springer Nature
ISBN: 3030728773
Category : Science
Languages : en
Pages : 354
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Book Description
This edited book provides an in-depth overview of carbon dioxide (CO2) transformations to sustainable power technologies. It also discusses the wide scope of issues in engineering avenues, key designs, device fabrication, characterizations, various types of conversions and related topics. It includes studies focusing on the applications in catalysis, energy conversion and conversion technologies, etc. This is a unique reference guide, and one of the detailed works is on this technology. The book is the result of commitments by leading researchers from various backgrounds and expertise. The book is well structured and is an essential resource for scientists, undergraduate, postgraduate students, faculty, R&D professionals, energy chemists and industrial experts.
Author: A.M. Martin
Publisher: Springer Science & Business Media
ISBN: 1461558212
Category : Science
Languages : en
Pages : 569
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Book Description
By covering both the general principles of bioconversion and the specific characteristics of the main groups of waste materials amenable to bioconversion methods, this new book provides the chemical, biochemical, agrochemical and process engineer with clear guidance on the use of these methods in devising a solution to the problem of industrial waste products.
