Investigation of Retention Mechanisms in Reversed-phase Liquid Chromatography with Reaction Kinetics PDF Download
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Author: Brian Scott Ludolph
Publisher:
ISBN:
Category :
Languages : en
Pages : 366
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Author: Brian Scott Ludolph
Publisher:
ISBN:
Category :
Languages : en
Pages : 366
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Author: Lay Choo Tan
Publisher:
ISBN:
Category :
Languages : en
Pages : 628
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Author: Thomas Gerard Burke
Publisher:
ISBN:
Category :
Languages : en
Pages : 98
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Author: Jun Dai
Publisher:
ISBN:
Category :
Languages : en
Pages : 626
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Author: Jennifer Mallette
Publisher:
ISBN:
Category :
Languages : en
Pages : 126
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Abstract: The experimental technique of mass spectrometric tracer pulse chromatography was used to investigate the uptake of RPLC eluents and a series of test analytes by a C (18subscript- ) bonded packing over the full element composition range. The experiments were carried out with binary eluents consisting of aqueous mixtures of acetonitrile, methanol, or tetrahydrofuran, as well as ternary eluents composed of acetonitrile, methanol, and water. The primary experimental data obtained for eluent components were in the form of excess volumes. The excess volume data were then used to determine the absolute volume of each eluent component in the stationary phase as a function of eluent composition. The absolute sorption isotherms of the eluent components were indirectly estimated by a combination of techniques specific to a limited eluent composition range. Regression analysis of the non-stationary inflection point of the excess isotherms provided an estimate of the volume of eluent sorbed by the stationary phase for organic-rich eluents. Three different assumptions were used and evaluated for the water-rich eluent compositions. The calculated void volume data were compared with the retention volume of thiourea, uracil, and acetone, solutes commonly used to estimate the volume of the mobile phase. The experimental results showed that water and thiourea did provide an accurate measure of the mobile phase volume but only over a limited range of eluent composition. Deuterated water was chosen as the unretained dead time marker for water rich eluents. With data for the water-rich eluents, the regression results from excess isotherm data was used to estimate the absolute volume of eluent sorbed by the stationary phase over the full eluent composition range. Retention factors for a series of test solutes were calculated with the thermodynamic and kinetic void volumes in order to determine the influence of immobilized eluent. It was hypothesized the retention factors of the test analytes would increase as the absolute amount of organic component in the eluent increased. However, retention factors continually decreased. Therefore, the effect of the uptake of eluent on the retention of test solutes appeared to be minimal for this particular set of test analytes. The experimental technique of mass spectrometric tracer pulse chromatography was shown to be a valid method for the investigation of the uptake of eluent by a C (subscript- ) bonded stationary phase, even with ternary eluent systems.
Author: M.C. García-Álvarez-Coque
Publisher: Elsevier Inc. Chapters
ISBN: 0128071311
Category : Science
Languages : en
Pages : 29
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The addition of reagents to an RPLC mobile phase enables the separation of ionizable compounds, inorganic anions, and metal ions using conventional instrumentation, silica-based materials, and hydro-organic mixtures, thanks to a variety of secondary equilibria. This gives rise to several chromatographic modes, whose main features are outlined in this chapter. The effect of the mobile phase pH on the retention of ionizable compounds is described, together with the recommended experimental practice. The mechanism of adsorption of amphiphilic anions or cations on the stationary phase to attract analytes with opposite charge or suppress the silanol activity is discussed. Different reagents, such as alkylammonium salts, surfactants (below and above the critical micelle concentration), perfluorinated carboxylate anions, chaotropic ions, and ionic liquids, are considered. The potential of metal chelation for the determination of metal ions and organic compounds is also summarized.
Author: Won Jo Cheong
Publisher:
ISBN:
Category : High performance liquid chromatography
Languages : en
Pages : 690
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Author: Lynn Alison Cole
Publisher:
ISBN:
Category :
Languages : en
Pages : 338
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Author: Salvatore Fanali
Publisher: Elsevier
ISBN: 0323999999
Category : Science
Languages : en
Pages : 886
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Book Description
Liquid Chromatography: Fundamentals and Instrumentation, Third Edition offers a single source of authoritative information on all aspects of the practice of modern liquid chromatography. The book gives those working in academia and industry the opportunity to learn, refresh, and deepen their understanding of the field by covering basic and advanced theoretical concepts, recognition mechanisms, conventional and advanced instrumentation, method development, data analysis, and more. This third edition addresses new developments in the field with updated chapters from expert researchers. The book is a valuable reference for research scientists, teachers, university students, industry professionals in research and development, and quality control managers. Emphasizes the integration of chromatographic methods and sample preparation Provides important data related to complex matrices, sample preparation, and data handling Gives background information to facilitate the choice of LC sub-technique and experimental conditions, mobile and stationary phases, detectors, data processing, and more Offers comprehensive updates to all chapters Includes new chapters on chiral recognition, co-solvents and mobile phase additives, physicochemical measurements, and identification and quantitation in mass spectrometry
Author: Eli Grushka
Publisher: CRC Press
ISBN: 142001806X
Category : Medical
Languages : en
Pages : 296
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Book Description
For more than four decades, scientists and researchers have relied on the Advances in Chromatography series for the most up-to-date information on a wide range of developments in chromatographic methods and applications. Volume 45 of this authoritative series once again compiles the work of expert contributors in order to present timely and cutting
