Assessment of Metal Concentrations and Species in Biologically Relevant Samples with Inductively Coupled Plasma Mass Spectrometric Techniques PDF Download
Are you looking for read ebook online? Search for your book and save it on your Kindle device, PC, phones or tablets. Download Assessment of Metal Concentrations and Species in Biologically Relevant Samples with Inductively Coupled Plasma Mass Spectrometric Techniques PDF full book. Access full book title Assessment of Metal Concentrations and Species in Biologically Relevant Samples with Inductively Coupled Plasma Mass Spectrometric Techniques by Katie DeNicola Cafferky. Download full books in PDF and EPUB format.
Author: Katie DeNicola Cafferky
Publisher:
ISBN: 9780549023142
Category :
Languages : en
Pages : 134
Get Book Here
Book Description
Lastly, arsenic contamination plagues many areas of Bangladesh. Evaluating the levels of this toxicant in cooked rice and vegetable samples from the region is important to fully measure the level of exposure. Determining the specific arsenic species in these samples is necessary to assess the true risk of such exposure.
Author: Katie DeNicola Cafferky
Publisher:
ISBN: 9780549023142
Category :
Languages : en
Pages : 134
Get Book Here
Book Description
Lastly, arsenic contamination plagues many areas of Bangladesh. Evaluating the levels of this toxicant in cooked rice and vegetable samples from the region is important to fully measure the level of exposure. Determining the specific arsenic species in these samples is necessary to assess the true risk of such exposure.
Author: Koon-Sing Ho
Publisher: Open Dissertation Press
ISBN: 9781361332351
Category :
Languages : en
Pages :
Get Book Here
Book Description
This dissertation, "Single-cell Analysis Using Inductively Coupled Plasma Mass Spectrometry" by Koon-sing, Ho, 何觀陞, was obtained from The University of Hong Kong (Pokfulam, Hong Kong) and is being sold pursuant to Creative Commons: Attribution 3.0 Hong Kong License. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation. All rights not granted by the above license are retained by the author. Abstract: The technique of single-cell analysis using time-resolved inductively coupled plasma-mass spectrometry has been characterized and optimized. Determination of the metal contents of individual cells provides data on the natural metal contents of the cells and the corresponding distributions in the population. The distribution is a useful indicator of the health and the state of development of the cells. The contents of sorbed metals of individual cells over a duration of time are required to understand the dynamics of metal-cell interactions. A green alga, Chlorella vulgaris, was used as a model biological cell in this study. The criteria and procedures for proper sampling of the cells into the ICP will be discussed. Ideally, each ICP-MS spike corresponds to one cell, but cell overlapping occurs because the cells enter the ICP randomly. Selection of cell number density and sample uptake rate to minimize spike overlapping will be discussed. A cell counting method based on the frequency of the spikes has been developed. The distribution of the metal contents of cells was determined by measuring large number of spikes. The minimum number of spikes required was determined by statistical analysis. The spike intensity distribution was correlated with the size distribution of the cells. The peak maximum of the spike intensity distribution was used for the determination of the average metal content of the cells. The use of the peak maximum reduces errors due to spike overlapping in the measurement. Quantitative determination of the metal contents was achieved using standard particles for calibration. Errors in calibration using standard solution nebulization were discussed. The technique was applied in the study of metal-cell interactions. Sorption of heavy metal ions (as environmental pollutants) by Chlorella vulgaris, and uptake of biometal (as nutrient) and metallodrug (as toxin) by Helicobacter pylori were studied. The technique requires simple sample preparation of removing the culture medium by filtration or centrifugation. The health state of the cells in the presence of toxic metals was related to the change in cell number density. The ratio of the FWHM of the spike intensity distributions of the sorbed metals to the natural metal contents of the cells is identified as a possible indicator of the location of the sorbed metals. The kinetics of metal sorption by the cells can be studied using a single cell culture. The method reduces errors due to uncertainties in cell number density and metal concentration in multiple samples that are required in conventional methods. The optimal ICP-MS sampling depth of 17 elements, introduced into the ICP by conventional solution nebulization of aqueous standard solutions, has been determined. The elements were selected to represent a wide range of boiling points and ionization potentials. Boiling point of the dried residues and ionization potential of the analyte element were identified as the major factors that determine the optimal sampling position. Since dried sample solution aerosols are effectively nanoparticles, the study provides useful insight on the optimization of the operation conditions and calibration strategies for single-particle analysis using ICP-MS. DOI: 10.5353/th_b4985851 Subjects: Inductively coupled plasma mass spectrometry Cells - Analysis
Author: G. Venkatesh Iyengar
Publisher: CRC Press
ISBN: 1000141721
Category : Science
Languages : en
Pages : 278
Get Book Here
Book Description
Despite the development of innovative new analytical techniques for biological trace element research, today's trace element investigators face formidable obstacles to obtaining reliable data. This complete reference identifies and assesses the challenges the analyst encounters at each stage of an analysis, and discusses the effects of various techniques on the sample. Three internationally recognized scientists and authors consider the effects of the numerous collection, storage, and sample preparatory techniques used in sample analysis. Proper analytical quality control, including such critical factors as sampling and sample preparation, specimen preservation and storage, and ashing, is examined. The book also looks at sample preparation methods unique to various instruments and speciation chemistry issues, and examines the link between chemical analysis and specimen banking. A previously unrecognized source of error, presampling factors, is also discussed.
Author: Barbara Cohrssen
Publisher: John Wiley & Sons
ISBN: 1119816203
Category : Science
Languages : en
Pages : 400
Get Book Here
Book Description
Since the first edition in 1948, Patty’s Industrial Hygiene and Toxicology has become a flagship publication for Wiley. During its nearly seven decades in print, it has become a standard reference for the fields of occupational health and toxicology. The volumes on industrial hygiene are cornerstone reference works for not only industrial hygienists but also chemists, engineers, toxicologists, lawyers, and occupational safety personnel. Volume 2 covers Chemical Exposure Evaluation and Control. Along with the updated and revised chapters from the prior edition, this volume has two new chapters: Sensor Technology and Control Banding.
Author: I.S. Krull
Publisher: Elsevier
ISBN: 0080858546
Category : Science
Languages : en
Pages : 319
Get Book Here
Book Description
The aim of this volume is to describe the most recent advances in areas of analytical chemistry that relate to the trace determination of metals and inorganics, as well as their distribution and forms (species) present, sample dependent. Analytical approaches are described that encompass a number of separation methods, such as gas and high performance liquid chromatography, interfaced with selective and sensitive detection methods that become unique for metal species/forms present in various samples. Hyphenated techniques are emphasized, such as interfacing HPLC with plasma induced emission spectroscopy, electrochemistry, post-column reaction chemistry, etc. Each chapter describes the latest instrumental and methodology advances that utilize some form of chromatography together with element-specific detection or mass spectrometry to provide absolute identification of the specific species of a metal present in various samples. The book will be of value to those concerned with the determination of trace levels of individual metal species present or suspected present in any given sample and to those involved in trace metal toxicology, metabolism of metal-containing drugs or chemicals, environmental exposures to metals and chemical speciation of real world samples. Government regulatory laboratories striving to detect and determine absolute levels of a metal species in any regulated sample will be interested in this volume, as will academic institutes involved in environmental toxicology, environmental chemistry, metal-DNA/protein interactions and researchers working with metal species.
Author: Roger L. Lundblad
Publisher: CRC Press
ISBN: 1315314436
Category : Medical
Languages : en
Pages : 1001
Get Book Here
Book Description
Edited by renowned protein scientist and bestselling author Roger L. Lundblad, with the assistance of Fiona M. Macdonald of CRC Press, this fifth edition of the Handbook of Biochemistry and Molecular Biology gathers a wealth of information not easily obtained, including information not found on the web. Presented in an organized, concise, and simple-to-use format, this popular reference allows quick access to the most frequently used data. Covering a wide range of topics, from classical biochemistry to proteomics and genomics, it also details the properties of commonly used biochemicals, laboratory solvents, and reagents. An entirely new section on Chemical Biology and Drug Design gathers data on amino acid antagonists, click chemistry, plus glossaries for computational drug design and medicinal chemistry. Each table is exhaustively referenced, giving the user a quick entry point into the primary literature. New tables for this edition: Chromatographic methods and solvents Protein spectroscopy Partial volumes of amino acids Matrix Metalloproteinases Gene Editing Click Chemistry
Author: Fattepurkar Sameer
Publisher: LAP Lambert Academic Publishing
ISBN: 9783659761331
Category :
Languages : en
Pages : 64
Get Book Here
Book Description
This book present the work that the study of extraction and analysis of heavy metals in fruit and vegetables by ICP MS. In the present study the concentrations of nine different heavy metals were determined in fruit and vegetable samples such as Grape, Pomegranate, Banana, Guava, Spinach and Onion which were freshly harvested in Pune region. Heavy metals were extracted from fruits and vegetables using Microwave digestion method and heavy metal concentrations were analyzed by using inductively coupled plasma mass spectrometry (ICP MS). The concentrations of As; Cd; Fe; Ni; Pb; Zn; Cr; Cu and Hg detected in samples. These values were lower than those recommended by FSSAI and WHO joint limits except Spinach (Cu 5.10) and Banana (Pb 0.26, As 1.5).
Author: Nader Rifai
Publisher: Elsevier Health Sciences
ISBN: 0323834671
Category : Medical
Languages : en
Pages : 4232
Get Book Here
Book Description
Use THE definitive reference for laboratory medicine and clinical pathology! Tietz Textbook of Laboratory Medicine, 7th Edition provides the guidance necessary to select, perform, and evaluate the results of new and established laboratory tests. Comprehensive coverage includes the latest advances in topics such as clinical chemistry, genetic metabolic disorders, molecular diagnostics, hematology and coagulation, clinical microbiology, transfusion medicine, and clinical immunology. From a team of expert contributors led by Nader Rifai, this reference includes access to wide-ranging online resources on Expert Consult — featuring the comprehensive product with fully searchable text, regular content updates, animations, podcasts, over 1300 clinical case studies, lecture series, and more. - Authoritative, current content helps you perform tests in a cost-effective, timely, and efficient manner; provides expertise in managing clinical laboratory needs; and shows how to be responsive to an ever-changing environment. - Current guidelines help you select, perform, and evaluate the results of new and established laboratory tests. - Expert, internationally recognized chapter authors present guidelines representing different practices and points of view. - Analytical criteria focus on the medical usefulness of laboratory procedures. - Use of standard and international units of measure makes this text appropriate for any user, anywhere in the world. - Elsevier eBooks+ provides the entire text as a fully searchable eBook, and includes animations, podcasts, more than 1300 clinical case studies, over 2500 multiple-choice questions, a lecture series, and more, all included with print purchase. - NEW! 19 additional chapters highlight various specialties throughout laboratory medicine. - NEW! Updated, peer-reviewed content provides the most current information possible. - NEW! The largest-ever compilation of clinical cases in laboratory medicine is included with print purchase on Elsevier eBooks+. - NEW! Over 100 adaptive learning courses included with print purchase on Elsevier eBooks+ offer the opportunity for personalized education.
Author: Zeev B. Alfassi
Publisher: John Wiley & Sons
ISBN: 3527615768
Category : Science
Languages : de
Pages : 622
Get Book Here
Book Description
The best way to determine trace elements! This easy-to-use handbook guides the reader through the maze of all modern analytical operations. Each method is described by an expert in the field. The book highlights the advantages and disadvantages of individual techniques and enables pharmacologists, environmentalists, material scientists, and food industry to select a judicious procedure for their trace element analysis.
Author: Us Epa
Publisher: CRC Press
ISBN: 9780873718318
Category : Science
Languages : en
Pages : 350
Get Book Here
Book Description
Methods for the Determination of Metals in Environmental Samples presents a detailed description of 13 analytical methods covering 35 analytes that may be present in a variety of sample types. The methods involve a wide range of analytical instrumentation including inductively coupled plasma (ICP)/atomic emission spectroscopy (AES), ICP/mass spectroscopy (MS), atomic absorption (AA) spectroscopy, ion chromatography (IC), and high performance liquid chromatography (HPLC). The application of these techniques to such a diverse group of sample types is a unique feature of this book. Sample types include waters ranging from drinking water to marine water, in addition to industrial and municipal wastewater, groundwater, and landfill leachate. The book also includes methods that will accommodate biological tissues, sediments, and soils. Methods in this book can be used in several regulatory programs because of their applicability to many sample types. For example, ICP/AES, ICP/MS, and AA methods can be used in drinking water and permit programs. Methods applicable to marine and estuarine waters can be used for the EPA's National Estuary Program. Terminology is consistent throughout the book, an important feature especially for the quality control sections where standardized terminology is not yet available. Methods for the Determination of Metals in Environmental Samples is an indispensable methods guide for all environmetal labs, wastewater labs, drinking water labs, lab managers, consultants, and groundwater engineers.
