Author: Claire Louise Poppett
Publisher:
ISBN:
Category : Fourier transform spectroscopy
Languages : en
Pages : 242

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Abstract: The instrumentation developments within this thesis are primarily aimed at instrumentation for the next generation of telescopes: Extremely large telescopes (ELTs). In the European astronomical community, the highest priority for ground-based optical and near-IR instrumentation has been identified as high-multiplex, multi-object spectroscopy (HMS) [1]. HMS includes both simultaneous observations of multiple faint objects at the limits of detection (Multiple Object Spectroscopy: MOS) and spatially-resolved spectroscopy over contiguous fields of brighter structured objects (Integral Field Spectroscopy: IFS) and a mixture of the two (Diverse Field Spectroscopy: DFS). However, before we can start to build instrumentation for ELTs it is important to: understand fibre characteristics more thoroughly and be able to predict behaviour with the use of a theoretical model (chapter 3); look at new technologies (Photonic Crystal Fibres, chapter 4, Volume Phase Holographic Gratings, chapter 5); use fibres in different ways (MAIFU, chapter 6).

Author: Kathleen Oram
Publisher:
ISBN:
Category : Hadamard transform spectroscopy
Languages : en
Pages : 0

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"Highly multiplexed spectroscopic capabilities are critical to future astronomy space missions. Such capabilities enable large samples of spectral data to be collected in an efficient manner. The individual mirrors of a Digital Micromirror Device (DMD) can serve as slits in a multi-object spectrograph (MOS). This work explores several areas vital to the inclusion of DMDs in future astronomy missions: space qualification, optical performance, and the implementation of Hadamard Transform Spectral Imaging (HTSI). While DMDs were not designed for space, this work reports on testing that demonstrates that the devices can withstand the environmental conditions of a space mission. The optical properties of a DMD ultimately drive the wavelength range and quality of spectral data obtained from a DMD-based MOS. We have characterized the reflectance and contrast ratio of various DMDs from near ultra-violet through visible wavelengths and discuss the results. This work also discusses efforts in expanding the spectral sensitivity of DMDs. Maximizing spectral information over a spatial field of view (FoV) on the sky is highly desirable. In the multi-object spectroscopy mode, individual DMD micromirrors are selected to generate a sparse sample of spectra at individual locations. Additionally, a DMD can be used for integral field spectroscopy (IFS) by forming a long slit from a line of micromirrors, which is then altered to effectively scan across the FoV. In this work we evaluate an alternative technique, HTSI. HTSI has the advantage of a gain in signal-to-noise ratio (SNR) as compared to direct measurements with a long slit, when the observed signals are not photon-noise dominated. We have simulated the performance of HTSI with a DMD-based MOS to identify the limitations of the technique and scenarios where it is most advantageous. With both MOS and IFS capabilities, a DMD-based instrument is a versatile asset fit for a variety of astronomy missions."--Abstract.

Author: Michael Kinter
Publisher: Springer Science & Business Media
ISBN: 1461486661
Category : Medical
Languages : en
Pages : 76

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Book Description
A key experiment in biomedical research is monitoring the expression of different proteins in order to detect changes that occur in biological systems under different experimental conditions. The method that is most widely used is the Western blot analysis. While Western blot is a workhorse in laboratories studying protein expression and has several advantages, it also has a number of significant limitations. In particular, the method is semi-quantitative with limited dynamic range. Western blot focuses on a single protein per sample with only a small number of representative samples analyzed in an experiment. New quantitative tools have been needed for some time to at least supplement, & possibly replace, the Western blot. Mass spectrometric methods have begun to compete with Western blot for routine quantitative analyses of proteins. One of these methods is based on the tandem mass spectrometry technique of selected reaction monitoring (SRM), which is also called multiple reaction monitoring (MRM). Selected reaction monitoring is actually an older tandem mass spectrometry technique, first described in the late 70s, that is widely utilized in the quantitative analysis of small molecules like drugs & metabolites. The use of selected reaction monitoring for the quantitative analysis of proteins has a number of advantages. Most importantly, it is fundamentally quantitative with a wide dynamic range. The output of the analysis is a numerical result that can range over several orders of magnitude. Other advantages include sufficient specificity & sensitivity to detect low abundance proteins in complex mixtures. Finally, selected reaction monitoring can be multiplexed to allow the quantitative analysis of relatively large numbers of proteins in a single sample in a single experiment. This Brief will explain both the theoretical & experimental details of the selected reaction monitoring experiment as it is applied to proteins. ​

Author: C. D. Anger
Publisher:
ISBN:
Category :
Languages : en
Pages :

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Author: EuroScicon
Publisher: EuroScicon
ISBN:
Category : Technology & Engineering
Languages : en
Pages : 90

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Book Description
April 26-27, 2018 Rome, Italy Key Topics : Nano Electronics, Nanotechnology For Clean Energy And Environment, Nano Applications, Nano Biotechnology, Nano Bio Medicine, Carbon And Graphene Nano-Structures, Polymer Science Engineering, Bio Polymers And Bio Plastics, Advanced Materials Science, Nano Composites, Nano Technology In Materials Science, Corrosion Engineering And Corrosion Protection, Biomaterials, Electronic, Optical & Magnetic Materials., Nano Photonics, Advanced Nano Materials,

Author: Eli Zamir
Publisher: Humana
ISBN: 9781071615928
Category : Science
Languages : en
Pages : 343

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Book Description
This volume provides a collection of state-of-the-art approaches addressing key aspects of multiplexed imaging. Chapters focus on labeling and imaging techniques for multiplexed imaging, as well as on the application of these techniques for the study of cells and tissues. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Multiplexed Imaging: Methods and Protocols aims to be helpful for researchers interested in implementing multiplexed imaging or in developing novel, cutting-edge multiplexed imaging approaches.

Author: Yuling Wang
Publisher: Elsevier
ISBN: 012823198X
Category : Science
Languages : en
Pages : 465

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Book Description
Principles and Clinical Diagnostic Applications of Surface-Enhanced Raman Spectroscopy summarizes the principles of surface-enhanced Raman scattering/spectroscopy (SERS) and plasmonic nanomaterials for SERS, with a focus on SERS applications in clinical diagnostics. This book covers the key concepts from the fundamentals, materials, experimental aspects, and applications of SERS in clinical diagnostics with discussions on label-free/direct SERS assay, design and synthesis of SERS nanotags, SERS nanotags for point-of-care diagnostics, microfluidic SERS assay, and in vitro and in vivo sensing and imaging. Written by experts from around the world, this comprehensive volume showcases the recent progress of SERS applications in clinical diagnostics and helps readers understand when and how to use SERS in a clinical setting. Introduces the basics of SERS and suitable nanomaterials for SERS application Gives an overview of the cutting-edge research on SERS applications for clinical diagnosis, including the latest advances in our understanding of underlying principles to enable material design and clinical applications Gradually builds from the fundamental concepts to the applications of SERS for clinical diagnostics

Author:
Publisher: Newnes
ISBN: 0444626700
Category : Science
Languages : en
Pages : 488

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Book Description
Fundamentals of Advanced Omics Technologies: From Genes to Metabolites covers the fundamental aspects of the new instrumental and methodological developments in omics technologies, including those related to genomics, transcriptomics, epigenetics, proteomics and metabolomics, as well as other omics approaches such as glycomics, peptidomics and foodomics. The principal applications are presented in the following complementary volume. The chapters discuss in detail omics technologies, DNA microarray analysis, next-generation sequencing technologies, genome-wide analysis of methylation and histone modifications, emerging nanotechniques in proteomics, imaging mass spectrometry in proteomics, recent quantitative proteomics approaches, and advances in high-resolution NMR-based metabolomics, as well as MS-based non-targeted metabolomics and metabolome analysis by CE-MS, global glycomics analyses, foodomics, and high resolution analytical tools for quantitative peptidomics. Key aspects related to chemometrics, bioinformatics, data treatment, data integration and systems biology, deep-sequencing data analysis, statistical approaches for the analysis of microarray data, the integration of transcriptome and metabolome data and computational approaches for visualization and integration of omics data are also covered. Covers the latest advances in instrumentation, experimental design, sample preparation, and data analysis Provides thorough explanations and descriptions of specific omics technologies Describes advanced tools and methodologies for data pretreatment, storage, curation and analysis, as well as data integration

Author:
Publisher: Academic Press
ISBN: 0128032251
Category : Science
Languages : en
Pages : 3716

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Book Description
This third edition of the Encyclopedia of Spectroscopy and Spectrometry, Three Volume Set provides authoritative and comprehensive coverage of all aspects of spectroscopy and closely related subjects that use the same fundamental principles, including mass spectrometry, imaging techniques and applications. It includes the history, theoretical background, details of instrumentation and technology, and current applications of the key areas of spectroscopy. The new edition will include over 80 new articles across the field. These will complement those from the previous edition, which have been brought up-to-date to reflect the latest trends in the field. Coverage in the third edition includes: Atomic spectroscopy Electronic spectroscopy Fundamentals in spectroscopy High-Energy spectroscopy Magnetic resonance Mass spectrometry Spatially-resolved spectroscopic analysis Vibrational, rotational and Raman spectroscopies The new edition is aimed at professional scientists seeking to familiarize themselves with particular topics quickly and easily. This major reference work continues to be clear and accessible and focus on the fundamental principles, techniques and applications of spectroscopy and spectrometry. Incorporates more than 150 color figures, 5,000 references, and 300 articles for a thorough examination of the field Highlights new research and promotes innovation in applied areas ranging from food science and forensics to biomedicine and health Presents a one-stop resource for quick access to answers and an in-depth examination of topics in the spectroscopy and spectrometry arenas

Author:
Publisher:
ISBN:
Category : Physics
Languages : en
Pages : 1288

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Book Description