Tissue Culture Techniques for Horticultural Crops PDF Download
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Author: Kenneth C. Torres
Publisher: Springer Science & Business Media
ISBN: 1461597560
Category : Technology & Engineering
Languages : en
Pages : 282
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Book Description
This book was written for those individuals who are concerned about the techniques and practices of plant cell cultures for horticultural crops. It was designed to serve as a text and reference for students and professionals in ornamental horticulture, fruit and vegetable crop pro duction, botany, forestry, and other areas of plant science. Research during the last twenty-five years in the area of plant tissue culture has led to many developments and changes in this field. Al though the techniques involved in the manipulation of plant tissue culture are now relatively straightforward, the presentation of these techniques in a short volume for the beginner in the field is generally unavailable. In addition to describing the techniques for establishment and manipulation of specific species, several chapters in this book also provide a brief, general review of important cultural parameters. Spe cific protocols and laboratory procedures may also be found in the appendix. I hope that this presentation of information will be helpful to those individuals wanting to apply plant tissue culture techniques for horticultural crops.
Author: Kenneth C. Torres
Publisher: Springer Science & Business Media
ISBN: 1461597560
Category : Technology & Engineering
Languages : en
Pages : 282
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Book Description
This book was written for those individuals who are concerned about the techniques and practices of plant cell cultures for horticultural crops. It was designed to serve as a text and reference for students and professionals in ornamental horticulture, fruit and vegetable crop pro duction, botany, forestry, and other areas of plant science. Research during the last twenty-five years in the area of plant tissue culture has led to many developments and changes in this field. Al though the techniques involved in the manipulation of plant tissue culture are now relatively straightforward, the presentation of these techniques in a short volume for the beginner in the field is generally unavailable. In addition to describing the techniques for establishment and manipulation of specific species, several chapters in this book also provide a brief, general review of important cultural parameters. Spe cific protocols and laboratory procedures may also be found in the appendix. I hope that this presentation of information will be helpful to those individuals wanting to apply plant tissue culture techniques for horticultural crops.
Author: Richard H. Zimmerman
Publisher: Springer Science & Business Media
ISBN: 9400944446
Category : Science
Languages : en
Pages : 369
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Book Description
In 1980, a conference on tissue culture of fruit crops was held at Beltsville to summarize the current status of this technology and to stimulate interest in it among research scientists, students, and commercial producers in the U. S. Interest in that conference and the proceedings from it far exceeded the expectations of the organizing committee. Since that time, micropropagation of fruit crops in the U. S. has increased significantly, but still lags far behind applications to production of ornamental plants. Within the past two years, a number of new laboratories have been established and some of the existing laboratories have expanded to a size far larger than any previously anticipated. Creation of new laboratories capable of producing more than 400,000 plants per week will test the ingenuity of laboratory managers and the skills of marketing departments. In recent years, numerous symposia have been held on various aspects of biotechnology and genetic engineering. Although micro propagation is the key to providing large numbers of genetically engineered plants, it is a topic that has been relegated to a minor position, or ignored completely, at such meetings. Accordingly, the time seemed propitious for a conference devoted solely to all aspects of micropropagation as applicable to horticultural crops.
Author: Shubhpriya Gupta
Publisher: Springer Nature
ISBN: 9811900558
Category : Science
Languages : en
Pages : 338
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Book Description
This edited book is focusing on the novel and innovative procedures in tissue culture for large scale production of plantation and horticulture crops. It is bringing out a comprehensive collection of information on commercial scale tissue culture with the objective of producing high quality, disease-free and uniform planting material. Developing low cost commercial tissue culture can be one of the best possible way to attain the goal of sustainable agriculture. Tissue culture provides a means for rapid clonal propagation of desired cultivars, and a mechanism for somatic hybridization and in vitro selection of novel genotypes. Application of plant tissue culture technology in horticulture and plantation crops provides an efficient method to improve the quality and nutrition of the crops. This book includes a description of highly efficient, low cost in vitro regeneration protocols of important plantation and horticulture crops with a detailed guideline to establish a commercial plant tissue culture facility including certification, packaging and transportation of plantlets. The book discusses somatic embryogenesis, virus elimination, genetic transformation, protoplast fusion, haploid production, coculture of endophytic fungi, effects of light and ionizing radiation as well as the application of bioreactors. This book is useful for a wide range of readers such as, academicians, students, research scientists, horticulturists, agriculturists, industrial entrepreneurs, and agro-industry employees.
Author: Roberta H. Smith
Publisher: Academic Press
ISBN: 0124159206
Category : Science
Languages : en
Pages : 206
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Book Description
Plant Tissue Culture, Third Edition builds on the classroom tested, audience proven manual that has guided users through successful plant culturing A.tumefaciens mediated transformation, infusion technology, the latest information on media components and preparation, and regeneration and morphogenesis along with new exercises and diagrams provide current information and examples. The included experiments demonstrate major concepts and can be conducted with a variety of plant material that are readily available throughout the year. This book provides a diverse learning experience and is appropriate for both university students and plant scientists. Provides new exercises demonstrating tobacco leaf infiltration to observe transient expression of proteins and subcellular location of the protein, and information on development of a customized protocol for protoplast isolation for other experimental systems Includes detailed drawings that complement both introductions and experiments Guides reader from lab setup to supplies, stock solution and media preparation, explant selection and disinfestations, and experimental observations and measurement Provides the latest techniques and media information, including A. tumefaciens mediated transformation and infusion technology Fully updated literature
Author: Robert H. Smith
Publisher: Academic Press
ISBN: 0323160476
Category : Science
Languages : en
Pages : 190
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Book Description
Plant Tissue Culture Techniques and Experiments is a manual that contains laboratory exercises about the demonstration of the methods and different plant materials used in plant tissue culture. It provides an overview on the plant cell culture techniques and plant material options in selecting the explant source. This book starts by discussing the proper setup of a tissue culture laboratory and the selection of the culture medium. It then explains the determination of an explant which is the ultimate goal of the cell culture project. The explant is a piece of plant tissue that is used in tissue culture. Furthermore, the book discusses topics about callus induction, regeneration and morphogenesis process, and haploid plants from anther and pollen culture. The meristem culture for virus-free plants and in vitro propagation for commercial propagation of ornamentals are also explained in this manual. The book also provides topics and exercises on the protoplast isolation and fusion and agrobacterium-mediated transformation of plants. This manual is intended for college students, both graduate and undergraduate, who study chemistry, plant anatomy, and plant physiology.
Author: B. N. Sathyanarayana
Publisher: I. K. International Pvt Ltd
ISBN: 8189866117
Category : Botany, Experimental
Languages : en
Pages : 299
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Book Description
Plant Tissue Culture forms an integral basis of the present day biotechnology. Plant Tissue Culture: Practices and New Experimental Protocols is being brought out to fill the existing gap in the available literature on plant tissue culture, especially focusing on the aspects of practical procedures and protocols of tissue culture. This book contains important experimental techniques and gives guidance on carrying out hands-on experiences. It has been designed in a simple way, giving all the necessary procedures as a general guideline and also necessary tips to maneuver any problem encountered. These tips are based on the first hand experiences of the author while teaching and researching the techniques of plant tissue culture. A unique feature of this book is the inclusion of several techniques describing the actual protocols experimented and developed with different plant species by different scientists. A substantial number of original colored plates including fluorescence photographs standout the book. This pioneering work is valuable for the students who are looking for fresh outlook and search.
Author: Avinash Chandra Rai
Publisher: Academic Press
ISBN: 0323998194
Category : Science
Languages : en
Pages : 428
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Book Description
Advances in Plant Tissue Culture: Current Developments and Future Trends provides a complete and up-to-date text on all basic and applied aspects of plant tissue cultures and their latest application implications. It will be beneficial for students and early-career researchers of plant sciences and plant/agricultural biotechnology. Plant tissue culture has emerged as a sustainable way to meet the requirements of fresh produces, horticultural crops, medicinal or ornamental plants. Nowadays, plant tissue culture is an emerging filed applied in various aspects, including sustainable agriculture, plant breeding, horticulture and forestry. This book covers the latest technology, broadly applied for crop improvement, clonal propagation, Somatic hybridization Embryo rescue, Germplasm conservation, genetic conservation, or for the preservation of endangered species. However, these technologies also play a vital role in breaking seed dormancy over conventional methods of conservation. Focuses on plant tissue culture as an emerging field applied in various aspects, including sustainable agriculture, plant breeding, horticulture and forestry Includes current studies and innovations in biotechnology Covers commercialization and current perspectives in the field of plant tissue culture techniques
Author: Randolph R. Henke
Publisher: Springer Science & Business Media
ISBN: 148990378X
Category : Technology & Engineering
Languages : en
Pages : 390
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Book Description
This symposium is the third in a series featuring the propaga tion of higher plants through tissue culture. The first of these symposia, entitled "A Bridge Between Research and Application," was held at the University in 1978 and was published by the Technical Information Center, Department of Energy. The second symposium, on "Emerging Technologies and Strategies," was held in 1980 and pub lished as a special issue of Environmental and Experimental Botany. One of the aims of these symposia was to examine the current state of-the-art in tissue culture technology and to relate this state of technology to practical, applied, and commercial interests. Thus, the third of this series on development and variation focused on embryogenesis in culture: how to recognize it, factors which affect embryogenesis, use of embryogenic systems, etc.; and variability from culture. A special session on woody species again emphasized somatic embryogenesis as a means of rapid propagation. This volume emphasizes tissue culture of forest trees. All of these areas, we feel, are breakthrough areas in which significant progress is expected in the next few years.
Author: Indra K. Vasil
Publisher: Springer Science & Business Media
ISBN: 9401726817
Category : Science
Languages : en
Pages : 593
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Book Description
Plant Cell and Tissue Culture gives an exhaustive account of plant cell culture and genetic transformation, including detailed chapters on all major field and plantation crops. Part A presents a comprehensive coverage of all necessary laboratory techniques for the initiation, nutrition, maintenance and storage of plant cell and tissue cultures, including discussions on these topics, as well as on morphogenesis and regeneration, meristem and shoot tip culture, plant protoplasts, mutant cell lines, variation in tissue cultures, isogenic lines, fertilization control, cryopreservation, transformation, and the production of secondary metabolites. Part B then proceeds into detail on the specific in vitro culture of specific crops, including cereals, legumes, vegetables, potatoes, other roots and tubers, oilseeds, temperate fruits, tropical fruits, plantation crops, forest trees and ornamentals. Plant Cell and Tissue Culture is, and is likely to remain, the laboratory manual of choice, as well as a source of inspiration and a guide to all workers in the field.
Author: Víctor M. Loyola-Vargas
Publisher: Springer Science & Business Media
ISBN: 1592599591
Category : Science
Languages : en
Pages : 389
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Book Description
A comprehensive state-of-the-art collection of the most frequently used techniques for plant cell and tissue culture. Readily reproducible and extensively annotated, the methods range from general methodologies, such as culture induction, growth and viability evaluation, and contamination control, to such highly specialized techniques as chloroplast transformation involving the laborious process of protoplast isolation and culture. Most of the protocols are currently used in the research programs of the authors or represent important parts of business projects aimed at the generation of improved plant materials. Two new appendices explain the principles for formulating culture media and the composition of the eight most commonly used media formulations, and list more than 100 very useful internet sites.
