The Role of LC and FAS in Regulating Floral Meristem and Fruit Locule Number in Tomato PDF Download
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Author: Yi-Hsuan Chu
Publisher:
ISBN:
Category : Botany
Languages : en
Pages :
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In tomato, lc and fas control the variation between the small and bilocular fruits from the wild ancestor (S. pimpinellifolium) and large fruit cultivars (S. lycopersicum var. lycopersicum) with up to ten locules. SlWUS and SlCLV3 are the candidates of lc and fas, respectively. The regulatory balance between these two genes plays a pivotal role in meristem maintenance in Arabidopsis. However, the genetic and molecular mechanisms of SlWUS and SlCLV3 have not been functionally characterized in tomato. Here, we performed a detailed phenotypic analysis of the reproductive organs in tomato near-isogenic lines. The results showed that lc and fas synergistically controlled floral organ and locule number. In addition, results from targeted RNA interference (RNAi) and transgenic complementation of fas clearly demonstrated that SlCLV3 was the gene underlying fas. By using mRNA in situ hybridization and transcriptome profiling, we observed temporal and spatial changes in the expression patterns of these two genes during floral development. Our results indicated that lc was a gain-of-function mutation of SlWUS while fas was a loss-of-function mutation of SlCLV3. We also conducted transcriptome analyses to capture differentially expressed genes (DEGs) responding to single and double mutations of lc and fas across vegetative/reproductive meristems and young flower buds. We adopted a recently developed 3’ Tag RNA-seq method for RNA-seq library preparation and compared its performance with the standard whole mRNA-seq method. Gene Ontology analysis over the DEGs showed enriched functionalities related to meristem/floral development and organ patterning. In addition, co-expression analysis revealed that microtubule motor activity and sterol/brassinosteroids biology might underlie differences between wild and fasciated tomato. In summary, the findings on tomato locule number and meristem control in this dissertation have provided new insights into the mechanisms in tomato fruit development.
Author: Yi-Hsuan Chu
Publisher:
ISBN:
Category : Botany
Languages : en
Pages :
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Book Description
In tomato, lc and fas control the variation between the small and bilocular fruits from the wild ancestor (S. pimpinellifolium) and large fruit cultivars (S. lycopersicum var. lycopersicum) with up to ten locules. SlWUS and SlCLV3 are the candidates of lc and fas, respectively. The regulatory balance between these two genes plays a pivotal role in meristem maintenance in Arabidopsis. However, the genetic and molecular mechanisms of SlWUS and SlCLV3 have not been functionally characterized in tomato. Here, we performed a detailed phenotypic analysis of the reproductive organs in tomato near-isogenic lines. The results showed that lc and fas synergistically controlled floral organ and locule number. In addition, results from targeted RNA interference (RNAi) and transgenic complementation of fas clearly demonstrated that SlCLV3 was the gene underlying fas. By using mRNA in situ hybridization and transcriptome profiling, we observed temporal and spatial changes in the expression patterns of these two genes during floral development. Our results indicated that lc was a gain-of-function mutation of SlWUS while fas was a loss-of-function mutation of SlCLV3. We also conducted transcriptome analyses to capture differentially expressed genes (DEGs) responding to single and double mutations of lc and fas across vegetative/reproductive meristems and young flower buds. We adopted a recently developed 3’ Tag RNA-seq method for RNA-seq library preparation and compared its performance with the standard whole mRNA-seq method. Gene Ontology analysis over the DEGs showed enriched functionalities related to meristem/floral development and organ patterning. In addition, co-expression analysis revealed that microtubule motor activity and sterol/brassinosteroids biology might underlie differences between wild and fasciated tomato. In summary, the findings on tomato locule number and meristem control in this dissertation have provided new insights into the mechanisms in tomato fruit development.
Author:
Publisher:
ISBN: 9789464470949
Category :
Languages : en
Pages : 184
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Author: Luz Stella Barrero Menses
Publisher:
ISBN:
Category :
Languages : en
Pages : 304
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Author: Zhongchi Liu
Publisher: Frontiers Media SA
ISBN: 2889194604
Category : Botany
Languages : en
Pages : 140
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Book Description
The fruit is an important plant structure. Not only does it provide a suitable environment for seeds to develop and serve as a vehicle for seed disposal, but it is also an indispensable part of the human diet. Despite its agronomic and nutritional value and centuries of intensive genetic selection, little is known about the molecular mechanism of its development or the evolution of its diverse forms. The last few years have witnessed a surge of investigations on the early stages of fruit development propelled by the advancement of high throughput sequencing technology, genome sequencing of fruit bearing species, and detailed molecular insights based on studies of model organisms. This research topic is focused on early stage fruit development that ranges from pre-fertilization patterning of the female ovary through post-fertilization fruit initiation and growth. Provided by the renowned experts in the field, these papers are intended to highlight recent progress and shed light on different aspects of fruit development from structure, function, to molecular genetics, and evolution.
Author: Xigang Liu
Publisher: Frontiers Media SA
ISBN: 2889767698
Category : Science
Languages : en
Pages : 148
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Author: Giovanna Frugis
Publisher: MDPI
ISBN: 3039281267
Category : Science
Languages : en
Pages : 246
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The way plants grow and develop organs significantly impacts the overall performance and yield of crop plants. The basic knowledge now available in plant development has the potential to help breeders in generating plants with defined architectural features to improve productivity. Plant translational research effort has steadily increased over the last decade due to the huge increase in the availability of crop genomic resources and Arabidopsis-based sequence annotation systems. However, a consistent gap between fundamental and applied science has yet to be filled. One critical point often brought up is the unreadiness of developmental biologists on one side to foresee agricultural applications for their discoveries, and of the breeders to exploit gene function studies to apply to candidate gene approaches when advantageous on the other. In this book, both developmental biologists and breeders make a special effort to reconcile research on the basic principles of plant development and organogenesis with its applications to crop production and genetic improvement. Fundamental and applied science contributions intertwine and chase each other, giving the reader different but complementary perspectives from only apparently distant corners of the same world.
Author: Rebecca Grumet
Publisher: Springer
ISBN: 3319493329
Category : Science
Languages : en
Pages : 434
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Book Description
This book provides an overview of the current state of knowledge of the genetics and genomics of the agriculturally important Cucurbitaceae plant family, which includes crops such as watermelon, melon, cucumber, summer and winter squashes, pumpkins, and gourds. Recent years have resulted in tremendous increases in our knowledge of these species due to large scale genomic and transcriptomic studies and production of draft genomes for the four major species, Citrullus lanatus, Cucumis melo, Cucumis sativus, and Cucurbita spp. This text examines genetic resources and structural and functional genomics for each species group and across species groups. In addition, it explores genomic-informed understanding and commonalities in cucurbit biology with respect to vegetative growth, floral development and sex expression, fruit growth and development, and important fruit quality traits.
Author: Masayoshi Shigyo
Publisher: Frontiers Media SA
ISBN: 2832502385
Category : Science
Languages : en
Pages : 205
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Author: Chittaranjan Kole
Publisher: CRC Press
ISBN: 1040022499
Category : Science
Languages : en
Pages : 495
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This book deliberates on the concept, strategies, tools, and techniques of allele mining in vegetable crops and its application potential in genome elucidation and improvement including studying allele evolution, discovery of superior alleles, discerning new haplotypes, assessment of intra- and interspecific similarity, and studies of gene expression and gene prediction. Available gene pools in global germplasm collections specifically consisting of wild allied species and local landraces for almost all major crops have facilitated allele mining. Development of advanced genomic techniques including PCR-based allele priming and Eco-TILLING based allele mining are being widely used now for mining superior alleles. Allele's discovery has become more relevant now for employing molecular breeding to develop designed crop varieties matching consumer needs and with genome plasticity to adapt the climate change scenarios. All these concepts and strategies along with precise success stories are presented in the chapters dedicated to the major vegetable crops. The first book on the novel strategy of allele mining in vegetable crops for precise breeding Presents genomic strategies for mining superior alleles underlying agronomic traits from genomic resources Depicts case studies of PCR-based allele priming and Eco-TILLING based allele mining Elaborates on gene discovery and gene prediction in major vegetable crops This book will be useful to the students and faculties in various plant science disciplines including genetics, genomics, molecular breeding, agronomy, and bioinformatics; the scientists in seed industries; and, the policymakers and funding agencies interested in crop improvement.
Author: Franco Famiani
Publisher: Frontiers Media SA
ISBN: 2889744981
Category : Science
Languages : en
Pages : 494
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