Author: A. van Soom
Publisher: Springer Science & Business Media
ISBN: 9781402005817
Category : Medical
Languages : en
Pages : 442
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Book Description
Thanks to enormous scientific efforts of the last decades, in vitro fertilization (IVF) and in vitro production (IVP) have now been introduced successfully in the practice of human infertility treatment and cattle breeding programs. This comprehensive book allows us to bridge the knowledge from both biomedical and veterinary fields of research. For the first time, studies concerning the human embryo as well as embryos from domestic species are brought together. The central theme of the book is "the assessment of mammalian embryo quality". In 15 chapters, written by well-known scientists, different aspects of the assessment of mammalian embryo quality are summarized. Non-invasive and invasive techniques to evaluate embryo quality are separated in two parts. In addition the book is provided with appendices on practical aspects and, thus, the book should be present in each laboratory for IVF and IVP.
Author: Patrick Quinn
Publisher: Cambridge University Press
ISBN: 1139917358
Category : Medical
Languages : en
Pages : 307
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Book Description
This volume describes culture media and solutions used in human ART; how they have been developed for in vitro human pre-implantation embryo development, the function and importance of the various components in media and solutions and how they interact, and how the systems in which these are used can influence outcomes. Chapters discuss inorganic solutes, energy substrates, amino acids, macromolecules, cytokines, growth factors, buffers, pH, osmolality, and the interaction of these parameters. The role of incubators and other physical factors are reviewed, along with the relevance and prospects of emerging technologies: morphokinetic analysis using time-lapse imaging and dynamic fluid incubation systems. Results of prospective randomized trials are emphasized to ascertain the added value of these techniques for selecting viable embryos. This comprehensive guide will be invaluable for embryologists, physicians and all personnel involved in the fluid products used in human ART seeking to optimize their successful use of these components.
Author: Efstratios M. Kolibianakis
Publisher: CRC Press
ISBN: 135167790X
Category : Medical
Languages : en
Pages : 434
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Book Description
Implantation is a complex phenomenon, still not thoroughly understood, involving the embryo and the endometrium. Successful implantation is considered to stem from an efficient combination of various embryo and/or maternal factors. Repeated failure of any of these factors or of their combination might decrease the chance of implantation and eventually lead to recurrent implantation failure. Despite technological advances, only about a quarter of In Vitro Fertilization procedures will result in live birth, leading many couples to experience multiple failures. This text from international experts explores the various factors at play and offers a helpful summary of the state of knowledge to help guide clinicians in their management of this complex and important problem. Contents: What is Recurrent Implantation Failure? * The psychosocial aspects of recurrent implantation failure * The role of life-style factors in RIF * Endocrine causes of recurrent implantation failure * Congenital uterine anomalies and recurrent implantation failure * Immunological causes of recurrent implantation failure * Acquired uterine conditions, reproductive surgery, and RIF? * Thrombophilia: diagnosis and management in women with recurrent implantation failure * Andrological causes of RIF * Diagnostic evaluation of RIF? (Modalities and algorithm) * Optimizing embryo culture for RIF? * Optimizing endometrial receptivity for patients with recurrent implantation failure: The role of progesterone on the day of triggering final oocyte maturation * Is there a role of follicular phase LH and E2 on the day of triggering final oocyte maturation for embryo implantation? * Manipulating the endometrium: Can RIF be managed by inducing endometrial inflammation or by the use of novel research therapies * The embryo in RIF: Genetic selection and strategies for improving its implantation potential * Is oocyte donation efficient in patients with RIF? * Sperm donation and RIF? * Optimizing embryo transfer technique for RIF management * Proposed management of patients with recurrent implantation failure and directions for future research * When should patients abandon treatment?
Author: David K. Gardner
Publisher: Oxford University Press
ISBN: 9780195142266
Category : Medical
Languages : en
Pages : 434
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Book Description
Never before has there been such a comprehensive book of protocols. This compendium offers a full range of research techniques-from cell culture, to biochemical, to microscopic and genetic. More focused books, like Cold Spring Harbor's Manipulating the Mouse Embryo, are similar though more narrow in scope. This book will appeal to a broad range of researchers, from basic experimental scientists to clinical and animal scientists.
Author: Barry D. Bavister
Publisher: Springer Science & Business Media
ISBN: 1461393175
Category : Science
Languages : en
Pages : 359
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Book Description
This volume contains the Proceedings of the Serono Symposium on Pre implantation Embryo Development, held in Newton, Massachusetts, in 1991. The idea for the symposium grew out of the 1989 Serono Symposium on Fertilization in Mammals* at which preimplantation development was the predominant suggestion for a follow-up topic. This was indeed a timely subject in view of the recent resurgence of interest in this funda mental phase of embryogenesis and its relevance to basic research and applied fertility studies in humans, food-producing animals, and endangered species. The symposium brought together speakers from a broad range of disciplines in order to focus on key regulatory mechanisms in embryo development, using a wide variety of animal models, and on representative topics in human preimplantation embryogenesis. The culmination of preimplantation development is a blastocyst con taining the first differentiated embryonic tissues and capable of initiating and sustaining pregnancy. The central objective of the symposium was to throw light on the regulation of cellular and molecular events underlying blastocyst formation. It was particularly appropriate that the date of the symposium marked the 20th anniversary of the publication of the classic volume Biology of the Blastocyst, the proceedings of an international workshop held in 1970. This book, which summarized most of the information then available on this topic in mammals, was edited by the pioneer in blastocyst research, Dr. Richard B1andau, who was the guest speaker at the symposium.
Author: Kersti Lundin
Publisher: Cambridge University Press
ISBN: 110887536X
Category : Medical
Languages : en
Pages : 125
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Book Description
Whilst assisted reproduction techniques (ART) have become increasingly successful and largely standardized, there is still only a partial understanding of what constitutes a 'true' embryo environment. Replicating the varying physiological conditions of the in-vivo environment that the embryo travels through in the in-vitro culture is still a major challenge in ART. This practical volume details how to organize and operate an IVF laboratory in order to mimic these conditions for successful embryo culture. Environments and equipment that are essential for running safe and efficient facilities such as maintaining good air quality and hygiene protocols, and utilizing an effective layout are covered in detail. Other chapters discuss the different consumables needed, optimal handling techniques and parameter monitoring systems, as well as recent advances in the area including artificial intelligence and automation. This is an indispensable guide to understanding the background science of culturing embryos, crucial to successful outcomes in ART.
Author: David K. Gardner
Publisher: Oxford University Press
ISBN: 019803251X
Category : Science
Languages : en
Pages : 409
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Book Description
This book pulls together the full range of cell culture, biochemical, microscopic, and genetic techniques to study the early mammalian embryo. Until now, there has never been such a comprehensive compendium, though there have been more focused books of protocol, such as Manipulating the Mouse Embryo, from Cold Spring Harbor. This book is intended to appeal to all constituencies, from basic experimental science to clinical and animal science applications.
Author: Gabor Kovacs
Publisher: Cambridge University Press
ISBN: 1108668461
Category : Medical
Languages : en
Pages : 402
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Book Description
This comprehensive review of the factors that affect the harvesting and preparation of oocytes and the management of embryos will allow practitioners to make evidence-based decisions for successful IVF. The book reviews and re-considers the value of strategies and outcomes in the management of fertility and conception rates, centred on the production of oocytes, and successful development of the embryo. Authored by leading experts in the field, chapters engage with treatments and strategies that affect the production of oocytes and embryos, optimizing outcomes in the management of female fertility, conception rates, and live births. This vital guide covers controlled ovarian hyperstimulation, the role of AMH in determining ovarian reserve, and primary stimulation agents and the use of adjuncts. Integral for all clinicians and embryologists working in reproductive medicine units, readers are provided with evidence-based, comprehensive advice and review of all factors affecting the management of oocytes and the embryo that are vital for successful IVF cycles.
Author: Kevin Coward
Publisher: Cambridge University Press
ISBN: 110727625X
Category : Medical
Languages : en
Pages : 408
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Book Description
The success of Assisted Reproductive Technology is critically dependent upon the use of well optimized protocols, based upon sound scientific reasoning, empirical observations and evidence of clinical efficacy. Recently, the treatment of infertility has experienced a revolution, with the routine adoption of increasingly specialized molecular biological techniques and advanced methods for the manipulation of gametes and embryos. This textbook – inspired by the postgraduate degree program at the University of Oxford – guides students through the multidisciplinary syllabus essential to ART laboratory practice, from basic culture techniques and micromanipulation to laboratory management and quality assurance, and from endocrinology to molecular biology and research methods. Written for all levels of IVF practitioners, reproductive biologists and technologists involved in human reproductive science, it can be used as a reference manual for all IVF labs and as a textbook by undergraduates, advanced students, scientists and professionals involved in gamete, embryo or stem cell biology.
Author: National Research Council
Publisher: National Academies Press
ISBN: 0309076374
Category : Science
Languages : en
Pages : 295
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Book Description
Human reproductive cloning is an assisted reproductive technology that would be carried out with the goal of creating a newborn genetically identical to another human being. It is currently the subject of much debate around the world, involving a variety of ethical, religious, societal, scientific, and medical issues. Scientific and Medical Aspects of Human Reproductive Cloning considers the scientific and medical sides of this issue, plus ethical issues that pertain to human-subjects research. Based on experience with reproductive cloning in animals, the report concludes that human reproductive cloning would be dangerous for the woman, fetus, and newborn, and is likely to fail. The study panel did not address the issue of whether human reproductive cloning, even if it were found to be medically safe, would beâ€"or would not beâ€"acceptable to individuals or society.
