RNA Polymerase III Transcription Initiation Complex Formation on a Yeast U6 RNA Gene PDF Download
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Author: Valerie Lynn Gerlach
Publisher:
ISBN:
Category :
Languages : en
Pages : 348
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Author: Valerie Lynn Gerlach
Publisher:
ISBN:
Category :
Languages : en
Pages : 348
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Author: Robert J. White
Publisher: Springer Science & Business Media
ISBN: 3662035189
Category : Science
Languages : en
Pages : 274
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Book Description
This monograph reviews and summarizes the substantial body of work that has been published on the transcription by polymerase III over the past 5 years. Progress in this field has been very rapid since 1993, and this new edition incorporates all the recent developments and offers the reader a highly detailed analysis of the current state of research on this largest and most complex of the eukaryotic RNA polymerases.
Author: Carolyne Bardeleben
Publisher:
ISBN:
Category :
Languages : en
Pages : 392
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Author: Ann Marie Moreno
Publisher:
ISBN:
Category :
Languages : en
Pages : 133
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In metazoans, the genes that code for the U6 small nuclear RNA (snRNA) have promoters that consist of a proximal sequence element (PSE) and a TATA box. The PSE is recognized by the multi-subunit small nuclear RNA activating protein complex (SNAPc). SNAPc forms a complex on the U6 promoter with TFIIIB, an RNA polymerase III-specific general transcription factor that includes TBP, Brf1 (Brf2 in vertebrates), and Bdp1. Here we show that, in the fruit fly Drosophila melanogaster, DmSNAPc directly recruits Bdp1 to the U6 promoter. We also demonstrate that an 87-residue region of Bdp1 is sufficient for this recruitment and is, furthermore, sufficient to recruit TBP to the TATA box. The non-conserved N-terminal tail of TBP also plays a role in stabilizing TBP incorporation into the DmSNAPc-Bdp1 complex. While Bdp1 recruitment by DmSNAPc is independent of the presence of TBP, Brf1, or a TATA box, it does require that DmSNAPc be bound to a U6 gene PSE rather than a PSE derived from a U1 gene (which is transcribed by RNA polymerase II). We also confirmed that Brf1 is present at U6 snRNA genes but not at U1 genes. These findings further develop the concept that DmSNAPc adopts different conformations upon binding U6 and U1 gene PSEs, and that these different DmSNAPc conformations lead to the subsequent recruitment of distinct general transcription factors and RNA polymerases for U6 and U1 gene transcription.
Author: Michael Robert Sturges
Publisher:
ISBN:
Category : Genetic transcription
Languages : en
Pages : 264
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Author: Rachel Imoberdorf
Publisher:
ISBN:
Category :
Languages : en
Pages : 130
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Transcription occurs in a repressive chromatin context. Chromatin modifications are therefore often required to allow transcriptional activation. Here, we addressed the question whether global histone acetylation is involved in the regulation of transcriptional activation of class II promoters. We show that global histone acetylation has a role in activation of RNA polymerase II dependent transcription by modulating the inherent inhibitory effects of chromatin on the formation of the pre-initiation complex. In a second project we have developed a chromatin immunoprecipitation based assay that allows the study of transcriptional reinitiation "in vivo". Our results suggest the formation of a reinitiation scaffold on the GAL1, but not the ACT1 promoter. In a third project assessed whether TFIIB reaches promoters in the form of the holoenzyme "in vivo". Our data indicate that TFIIB does not assemble in a holoenzyme but associates with another, yet unidentified, factor(s) prior to promoter binding.
Author: Sankar Adhya
Publisher: Elsevier
ISBN: 0080522599
Category : Science
Languages : en
Pages : 833
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RNA polymerase is molecule important to gene transcription. Along with associated factors, RNA polymerase is part of the process in which RNA is transcribed to produce a protein.* Construction and purification of RNA polymerases* DNA microarrays and bacterial gene expression* Functional analysis of transcription factors
Author: Brian A Larkins
Publisher: CABI
ISBN: 178639121X
Category : Technology & Engineering
Languages : en
Pages : 255
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Book Description
This is an authoritative book that acts as a guide to understanding maize kernel development. Written by a team of experts, it covers topics spanning pre- and post-fertilization events, embryo and endosperm development, grain filling and maturation, and factors influencing crop yield. It explores the significance of maize and other cereal grains, existing hypotheses and research, and important gaps in our knowledge and how we might fill them. This is a valuable resource for researchers of maize and other cereals, and anyone working on basic or applied science in the fields of seed development, plant genetics, and crop physiology.
Author: J. Robin Harris
Publisher: Springer Nature
ISBN: 3030281515
Category : Science
Languages : en
Pages : 657
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Book Description
This book follows on from Volume 83 in the SCBI series (“Macromolecular Protein Complexes”), and addresses several important topics (such as the Proteasome, Anaphase Promoting Complex, Ribosome and Apoptosome) that were not previously included, together with a number of additional exciting topics in this rapidly expanding field of study. Although the first SCBI Protein Complex book focused on soluble protein complexes, the second (Vol. 87)addressed Membrane Complexes, and the third (Vol. 88) put the spotlight on Viral Protein and Nucleoprotein Complexes, a number of membrane, virus and even fibrillar protein complexes have been be considered for inclusion in the present book. A further book is also under preparation that follows the same pattern, in an attempt to provide a thorough coverage of the subject. Chapter 9 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Author: Xianzhou Song
Publisher:
ISBN:
Category : Genetic transcription
Languages : en
Pages : 200
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Book Description
