Rapid Immunoassays by Capillary Zone Electrophoresis with Laser- Induced Fluorescence Detection PDF Download
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Author: Nicole M. Schultz
Publisher:
ISBN:
Category : Capillary electrophoresis
Languages : en
Pages : 284
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Author: Nicole M. Schultz
Publisher:
ISBN:
Category : Capillary electrophoresis
Languages : en
Pages : 284
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Author: Liling Zhang
Publisher:
ISBN:
Category :
Languages : en
Pages : 80
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Author: Jianping Ou
Publisher:
ISBN: 9781374748651
Category :
Languages : en
Pages :
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Author: Jianping Ou
Publisher:
ISBN:
Category : Capillary electrophoresis
Languages : en
Pages : 272
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Author: Jian Ying Zhao
Publisher:
ISBN:
Category :
Languages : en
Pages : 380
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Author:
Publisher:
ISBN:
Category :
Languages : en
Pages : 14
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The Canadian Forces require rapid, sensitive systems for identification of bacterial & viral biological warfare (BW) agents in environmental matrices such as air and water. The system must detect BW agents under battlefield conditions at extremely low concentrations with no false alarms. Together, capillary zone electrophoresis with laser induced fluorescent detection (CE-LIF) of gene probe fragments offers the potential for developing a sensitive BW agent identification capability. Gene probes can be used to target signature sequences of BW agents for identification, and CE-LIF detection is an attractive technology for detection of nucleic acid fragments because of its ultra-low detection limits and relatively simple instrument design. This paper describes experiments using a probe for Bacillus globigii, an anthrax simulant, to demonstrate the capabilities of CE-LIF technology with regard to identifying target DNA, the detection limits achieved, ease of use, and speed of detection compared to other methods.
Author: Mark A. Strege
Publisher: Springer Science & Business Media
ISBN: 159259798X
Category : Science
Languages : en
Pages : 340
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Book Description
Throughout the more than 20 years that have followed the beginnings of capillary electrophoresis (CE), its application to the analysis of proteins and peptides has continued to be reliable, versatile, and productive. Over time, CE has matured to become a superb complement to HLPC, and in many cases has also evolved as an automated and quantitative replacement for conventional slab gel electrophoresis methods such as SDS-PAGE and isoelectric focusing. Within Capillary Electrophoresis of Proteins and Peptides, we have assembled contributions from researchers who are applying state-of-the-art CE for protein and peptide analysis, including topics that we believe are of great potential both in the present and for the future. In comparison to traditional separation methods, CE represents a miniaturized analysis technique (especially in its microchip-based format) that is highly dependent upon the basic fundamentals of effective sample recovery and high sensitivity detection. With these issues in mind, Chapters 1–4 describe recently developed approaches for both capillary coatings and analyte detection via laser-induced fluorescence. Since the discipline of biotechnology has established itself as a primary platform for the application of CE to the analysis of proteins and peptides, Chapters 5–7 demonstrate a variety of examples of the specific techniques that have been applied for the development of biopharmaceuticals and their commercialization. The methods covered here include also the analysis of oligosaccharides from glycoproteins.
Author: James P. Landers
Publisher: CRC Press
ISBN: 9780849324987
Category : Medical
Languages : en
Pages : 918
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Book Description
Because new information was discovered at an incredible rate since the publication of the successful first edition of this Handbook, this fully updated second edition covers all areas of interest in the field of capillary electrophoresis (CE). A relatively new technology, CE is a principle method for studying the physicochemical properties of proteins, peptides, and other macromolecules. Where applicable, the 30 chapters provide basic underlying theories as well as application-oriented aspects of each technique.Keep up with all the developments in this growing field with the Handbook of Capillary Electrophoresis, Second Edition - a complete guide to the fundamentals of CE and the latest research. The chapters are organized into five units: Modes: Presents a theoretical development of the basic principles governing separation with several modes, including CEC, and discusses their practical aspects. Analyte: Applies CE to the analysis of a specific class of analytes, including organic and inorganic ions, pharmaceuticals, glycoconjugates, peptides, proteins, and DNA fragments. Fundamental Aspects of CE: Technique-oriented information for the practitioner, including the importance of the sample matrix, on-line preconcentration of samples, modes of detection, and specific aspects of CE data analysis. Applications of CE: Includes single cell analysis, CE in DNA sequencing, CE as a clinical diagnostic tool, identifying and quantifying drugs, and for characterizing interacting species. Specialized Aspects of CE: Discusses interfacing CE with mass spectrometry, high-volume throughput continuous CE, microchip CE, control of EOF, and much more. The Handbook of Capillary Electrophoresis, Second Edition, pulls together diverse areas and applications of CE, resulting in an excellent tool for scientists involved in biotechnology and clinical chemistry, as well as the pharmaceutical, bioscience, chemical, and instrument-manufacturing industries. With an applications-oriented focus, the handbook is also a superb manual for workshops, seminars, and graduate courses in separation science.
Author: Li Tao
Publisher:
ISBN:
Category : Capillary electrophoresis
Languages : en
Pages : 362
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Author:
Publisher:
ISBN:
Category :
Languages : en
Pages :
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A means and method for capillary zone electrphoresis with laser-induced indirect fluorescence detection. A detector is positioned on the capillary tube of a capillary zone electrophoresis system. The detector includes a laser which generates a laser beam which is imposed upon a small portion of the capillary tube. Fluorescence of the elutant electromigrating through the capillary tube is indirectly detected and recorded.
