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Author: Seema Anil Belorkar
Publisher: Academic Press
ISBN: 0323914373
Category : Science
Languages : en
Pages : 268
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Book Description
Protocols and Applications in Enzymology provides instruction on the experimental procedures of enzyme isolation techniques, innovative screening techniques, and instrument enabled enzyme assays and their underlying principles, among other protocols. The book serves as a one-stop solution for those working with different enzyme protocols in the fields of biochemistry, microbiology, biotechnology and allied subjects. Each chapter offers a full overview of protocol key resources, materials required, quantifiable and statistical analysis, optimization and troubleshooting, safety considerations, and standards. Applications are discussed across distribution and diversity of microbial enzymes, enzyme screening, enzymes in solid state fermentations, enzyme assays, enzyme kinetics, and biotechnological uses. Provides step-by-step instruction on enzyme protocols and applications, with actionable discussions of needed resources, materials, quantification and statistical analysis, optimization and troubleshooting, safety considerations and standards Presents easy to read, reproducible protocols for researchers and students across academia and industry Includes color diagrams that illustrate key concepts
Author: Seema Anil Belorkar
Publisher: Academic Press
ISBN: 0323914373
Category : Science
Languages : en
Pages : 268
Get Book Here
Book Description
Protocols and Applications in Enzymology provides instruction on the experimental procedures of enzyme isolation techniques, innovative screening techniques, and instrument enabled enzyme assays and their underlying principles, among other protocols. The book serves as a one-stop solution for those working with different enzyme protocols in the fields of biochemistry, microbiology, biotechnology and allied subjects. Each chapter offers a full overview of protocol key resources, materials required, quantifiable and statistical analysis, optimization and troubleshooting, safety considerations, and standards. Applications are discussed across distribution and diversity of microbial enzymes, enzyme screening, enzymes in solid state fermentations, enzyme assays, enzyme kinetics, and biotechnological uses. Provides step-by-step instruction on enzyme protocols and applications, with actionable discussions of needed resources, materials, quantification and statistical analysis, optimization and troubleshooting, safety considerations and standards Presents easy to read, reproducible protocols for researchers and students across academia and industry Includes color diagrams that illustrate key concepts
Author: James Samuelson
Publisher: Humana Press
ISBN: 9781493962877
Category : Science
Languages : en
Pages : 266
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Book Description
This book provides guidance to those wishing to create enzyme variants. It covers such topics as a simple method for generating site-specific mutations within bacterial chromosomes and the engineering of two difference types of rare-cutting endonucleases.
Author: Munishwar N. Gupta
Publisher: Birkhäuser
ISBN: 3034884729
Category : Science
Languages : en
Pages : 229
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Book Description
Extending the range of enzymatic catalysis by using non-aqueous media has now developed into a powerful approach in biochemistry and biotechnology. One peculiar feature which distinguishes it from the conventional enzymology (carried out in aqueous buffers) is that the awareness of different parameters that control and influence the behaviour of enzymes in such environments has emerged rather slowly. Science is about being able to repeat what somebody else has done. Absence of knowledge about such well-defined parameters/fac tors has sometimes made some workers rather cautious and diffident about using this approach in their laboratories. But for this, non-aqueous enzymol ogy would be more widely practised. It is these thoughts that made me feel that the availability of some well-defined protocols for various applications invol ving enzymes in non-aqueous environments would further catalyze the growth of this area. Hence this book, in which each chapter has some protocols in a specific area. The protocols are preceded by brief background material. The early chapters, which are of general importance, concern control of water ac tivity and stabilization via immobilization. Some subsequent chapters provide the protocols for transformations involving lipids and carbohydrates, peptide synthesis, and preparation of chiral compounds. The disproportionate focus on lipases is not a coincidence; this class of enzymes has been used more often than others in non-aqueous enzymology.
Author: Shelley D. Minteer
Publisher: Humana Press
ISBN: 9781607618942
Category : Science
Languages : en
Pages : 0
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Book Description
In the past decade, researchers have made tremendous progress in the field of enzyme stabilization, opening up new opportunities for enzymes in molecular biology and for industrial applications. In Enzyme Stabilization and Immobilization: Methods and Protocols, expert researchers explore the latest developments through detailed laboratory protocols, which address many different theories and techniques in enzyme stabilization. Chapters outline protocols for enzyme stabilization in solutions, including: liposome formation, micelle introduction, crosslinking, and additives. Secondly, the book contain protocols for enzyme stabilization via enzyme immobilization, such as sol-gel encapsulation, polymer encapsulation, and single enzyme nanoparticle formation. Composed in the highly successful Methods in Molecular BiologyTM series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and current, Enzyme Stabilization and Immobilization: Methods and Protocols is an essential handbook for all molecular biologists, biochemists, and biomedical and biochemical engineers.
Author: Hans Bisswanger
Publisher: John Wiley & Sons
ISBN: 3527820809
Category : Science
Languages : en
Pages : 533
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Book Description
A practice-oriented guide to assaying more than 100 of the most important enzymes, complete with the theoretical background and specific protocols for immediate use in the biochemical laboratory. Now expanded with a new section on metal ion determination.
Author: Haralambos Stamatis
Publisher:
ISBN: 9781071622698
Category : Multienzyme complexes
Languages : en
Pages : 379
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Book Description
This volume details cutting-edge methods and protocols for the development, characterization, and applications of multienzyme assemblies. Chapters guide readers through up-to-date techniques applied for the development and emerging applications of multi-enzymatic systems for biotransformations, biosensing, molecular-scale diagnostics and bioelectronics. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Multienzymatic Assemblies: Methods and Protocols aims to be useful for academics and industry professionals working in the field of biotechnology, biochemistry, chemical and biological engineering, nanobiotechnology, and biocatalysis.
Author: Carl Wu
Publisher:
ISBN:
Category : Chromatin
Languages : en
Pages :
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Book Description
Author: Jon Lorsch
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Book Description
These volumes of Methods in Enzymology contain the protocols that made up the on-line Methods Navigator. Our philosophy when we selected the protocols to include in the Navigator was that they should be for techniques useful in any biomedical laboratory, regardless of the system the lab studies. Each protocol was written by researchers who use the technique routinely, and in many cases by the people who actually developed the procedure in the first place. The protocols are very detailed and contain recipes for the necessary buffers and reagents, as well as flow-charts outlining the steps involved. Many of the chapters have accompanying videos demonstrating key parts of the procedures. The volumes are broken into distinct areas: DNA methods; Cell-based methods; lipid, carbohydrate and miscellaneous methods; RNA methods; Protein methods. Our goal is that these protocols will be useful for everyone in the lab, from undergraduates and rotation students to seasoned post-doctoral fellows. We hope that these volumes will become dog-eared and well-worn in your laboratory, either physically or electronically.
Author: Alane Beatriz Vermelho
Publisher: Bentham Science Publishers
ISBN: 1608053008
Category : Science
Languages : en
Pages : 334
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Book Description
Methods to Determine Enzymatic Activity is a textbook about industrial enzymes. The book features definitions, classifications and applications of selected enzymes important in industry and in biotechnological processes. Analytical methods for these enzymes are also included in the text. The main objective of this textbook is to provide readers information focused on the current analysis methods of enzymatic activity at qualitative and quantitative levels. Each chapter is about one specific enzyme and contains information about its substrate and some biochemical properties. The methodologies are presented as an experimental protocol allowing interested readers to reproduce the experimental methods detailed within the textbook. These protocols contain the principle of the technique, materials, methods, and all steps necessary for the determination of enzyme activity and interpretation of results. Each methodology is illustrated with photos and schemes for a better and clear understanding. This book, therefore, uniquely brings modern analysis techniques of industrial enzymes in a single easy to understand volume. This textbook is suitable for undergraduate enzymology courses and advanced industrial biotechnology and microbiology courses.
Author: Selim Kermasha
Publisher: Academic Press
ISBN: 0128005076
Category : Technology & Engineering
Languages : en
Pages : 330
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Book Description
Enzymes: Novel Biotechnological Approaches for the Food Industry provides an in-depth background of the most up-to-date scientific research and information related to food biotechnology and offers a wide spectrum of biological applications. This book addresses novel biotechnological approaches for the use of enzymes in the food industry to help readers understand the potential uses of biological applications to advance research. This is an essential resource to researchers and both undergraduate and graduate students in the biotechnological industries. Provides fundamental and rigorous scientific information on enzymes Illustrates enzymes as tools to achieve value and quality to a product, either in vitro or in vivo Presents the most updated knowledge in the area of food biotechnology Demonstrates novel horizons and potential for the use of enzymes in industrial applications
