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Author: Kirankumar S. Mysore
Publisher: Humana
ISBN: 9781493948475
Category : Science
Languages : en
Pages : 0
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Book Description
This volume provides readers with a comprehensive review of various gene silencing methodologies and its applications. It provides a historical overview of gene silencing mechanisms in plants, vectors, and strategies available for plant gene silencing, practical applications of gene silencing, bioinformatics tools, and other resources. In addition to these review chapters, this book includes methodology for virus-induced gene silencing (VIGS) in various different plant species, understanding plant stress responses using VIGS, miRNA identification, DNA interference, host-induced gene silencing, use of artificial miRNAs for gene silencing, high throughput RNAi, and more. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Thorough and practical, Plant Gene Silencing: Methods and Protocols seeks to aid scientists to further understand functional relevance of target genes using gene silencing methods and use these technologies in commercial plant varieties.
Author: Kirankumar S. Mysore
Publisher: Humana
ISBN: 9781493948475
Category : Science
Languages : en
Pages : 0
Get Book Here
Book Description
This volume provides readers with a comprehensive review of various gene silencing methodologies and its applications. It provides a historical overview of gene silencing mechanisms in plants, vectors, and strategies available for plant gene silencing, practical applications of gene silencing, bioinformatics tools, and other resources. In addition to these review chapters, this book includes methodology for virus-induced gene silencing (VIGS) in various different plant species, understanding plant stress responses using VIGS, miRNA identification, DNA interference, host-induced gene silencing, use of artificial miRNAs for gene silencing, high throughput RNAi, and more. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Thorough and practical, Plant Gene Silencing: Methods and Protocols seeks to aid scientists to further understand functional relevance of target genes using gene silencing methods and use these technologies in commercial plant varieties.
Author: M.A. Matzke
Publisher: Springer Science & Business Media
ISBN: 9401141835
Category : Science
Languages : en
Pages : 297
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Book Description
This book is an up-to-date and comprehensive collection of reviews on various aspects of epigenetic gene silencing in plants. Research on this topic has undergone explosive growth during the past decade and has revealed novel features of gene regulation and plant defense responses that also apply to animals and fungi. Gene silencing is relevant for agricultural biotechnology because stable expression of transgenes is required for the successful commercialization of genetically engineered crops. The reviews have been written by distinguished authors who have made significant contributions to plant gene silencing research. This volume supersedes other books on gene silencing by focussing on plant systems, where many pioneering experiments have been performed, and by including the latest developments from top laboratories. The book is geared toward advanced students of genetics and plant sciences as well as applied and basic research scientists who work with transgenic organisms and epigenetic regulation of gene expression.
Author: Tamas Dalmay
Publisher: CABI
ISBN: 1780647670
Category : Science
Languages : en
Pages : 225
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Book Description
Plant gene silencing is a crucially important phenomenon in gene expression and epigenetics. This book describes the way small RNA is produced and acts to silence genes, its likely origins in defence against viruses, and also its potential to improve plants. Plant gene silencing can be used to improve industrial traits, make plants more nutritious or more valuable to consumers, to remove allergens, and to improve resistance to weeds and pathogens.
Author: Charles H. Calisher
Publisher: Springer Science & Business Media
ISBN: 3709164257
Category : Medical
Languages : en
Pages : 222
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Book Description
One hundred years ago, when Martinus W. Beijerinck in Delft and Friedrich Loeffler on Riems Island discovered a new class of infectious agents in plants and animals, a new discipline was born. This book, a compilation of papers written by well-recognized scientists, gives an impression of the early days, the pioneer period and the current state of virology. Recent developments and future perspectives of this discipline are sketched against a historic background. With contributions by A. Alcami, D. Baulcombe, F. Brown, L. W. Enquist, H. Feldmann, A. Garcia-Sastre, D. Griffiths, M. C. Horzinek, A. van Kammen, H.-D. Klenk, F. A. Murphy, T. Muster, R. O'Neill, P. Palese, C. Patience, R. Rott, H.- P. Schmiedebach, S. Schneider-Schaulies, G. L. Smith, J. A. Symons, Y. Takeuchi, V. ter Meulen, P. J. W. Venables, V. E. Volchkov, V. A. Volchkova, R. A. Weiss, W. Wittmann, H. Zheng.
Author: Annette Becker
Publisher: Humana Press
ISBN: 9781627032773
Category : Medical
Languages : en
Pages : 0
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Book Description
Plants are amazing organisms to study, some are important sources for pharmaceuticals, and others can help to elucidate molecular mechanisms required for a plant’s development and its interactions with the biotic or abiotic environment. Functional genomics is vastly lagging behind the speed of genome sequencing as high-throughput gene function assays are difficult to design, specifically for non-model plants. Bioinformatics tools are useful for gene identification and annotation but are of limited value for predictions concerning gene functions as gene functions are uncovered best by experimental approaches. Virus-Induced-Gene-Silencing (VIGS) is an easy to use, fast, and reliable method to achieve down regulation of target gene expression. Virus-Induced Gene Silencing: Methods and Protocols provides detailed protocols for VIGS experiments in several plant species including model and non-model plants. Also included in this book are recently developed protocols for VIGS-derived microRNA production in the plant or protein over expression, as well as chapters devoted to summarizing the molecular mechanisms of VIGS action and the vector systems developed so far. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Virus-Induced Gene Silencing: Methods and Protocols serves as a valuable resource for researchers from diverse fields of plant biology interested in experimental approaches to analyzing gene functions.
Author: Kirankumar S. Mysore
Publisher: Humana
ISBN: 9781071618745
Category : Science
Languages : en
Pages : 328
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Book Description
This second edition provides a comprehensive review of various gene silencing methodologies and applications. Chapters detail a historical overview of gene silencing mechanisms in plants, vectors, and strategies available for plant gene silencing, practical applications of gene silencing, bioinformatics tools, and other resources. In addition to these review chapters, this book includes methodology for virus-induced gene silencing (VIGS), understanding plant stress responses using VIGS, miRNA identification, DNA interference, host-induced gene silencing, artificial miRNAs for gene silencing, and high throughput RNAi. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Plant Gene Silencing: Methods and Protocols, Second Edition aims to further the understanding of functional relevance of target genes using gene silencing methods and technologies in commercial plant varieties.
Author: Peter Meyer
Publisher: Springer Science & Business Media
ISBN: 364279145X
Category : Science
Languages : en
Pages : 237
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Book Description
In recent years several different gene silencing phenomena have been discovered in plants. The book summarizes the most recent data on gene silencing phenomena such as trans-, inactivation, paramutation and co-suppression. Plant researchers will find this edition a valuable help in differentiating between a number of puzzling and partly contradictory gene silencing events. Those not familiar with plant molecular biology are introduced into the relevant methods and scientific models. In addition examples and models of gene silencing in flamentous fungi, Drosophila and mammalian systems are presented. By providing a comparative update on gene silencing effects in different eukaryotes, this book should stimulate communication among scientists working in diverse areas of eukaryotic gene regulation.
Author: Praveen Guleria
Publisher: Academic Press
ISBN: 012817336X
Category : Science
Languages : en
Pages : 638
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Book Description
Plant Small RNA: Biogenesis, Regulation and Application describes the biosynthesis of small RNA in plant systems. With an emphasis on the various molecular mechanisms affected by small RNA and their applications in supporting plant growth and survival, this books presents the basics and most recent advancements in small RNA mediated plant genomics, metabolomics, proteomics and physiology. In addition, it emphasizes the various molecular mechanisms affected by small RNA and their applications in supporting plant growth and survival. Final sections cover the most recent advancements in small RNA mediated plant genomics, metabolomics, proteomics and physiology. - Presents foundational information about small RNA biology and regulation in plants - Includes small RNA pathway advances - Describes the application and scope of small RNA technology for agricultural stability
Author: Renato Paro
Publisher: Springer Nature
ISBN: 3030686701
Category : Science
Languages : en
Pages : 215
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Book Description
This open access textbook leads the reader from basic concepts of chromatin structure and function and RNA mechanisms to the understanding of epigenetics, imprinting, regeneration and reprogramming. The textbook treats epigenetic phenomena in animals, as well as plants. Written by four internationally known experts and senior lecturers in this field, it provides a valuable tool for Master- and PhD- students who need to comprehend the principles of epigenetics, or wish to gain a deeper knowledge in this field. After reading this book, the student will: Have an understanding of the basic toolbox of epigenetic regulation Know how genetic and epigenetic information layers are interconnected Be able to explain complex epigenetic phenomena by understanding the structures and principles of the underlying molecular mechanisms Understand how misregulated epigenetic mechanisms can lead to disease
Author: Noureddine Benkeblia
Publisher: John Wiley & Sons
ISBN: 1119079942
Category : Technology & Engineering
Languages : en
Pages : 549
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Book Description
An in-depth treatment of cutting-edge work being done internationally to develop new techniques in crop nutritional quality improvement Phytonutritional Improvement of Crops explores recent advances in biotechnological methods for the nutritional enrichment of food crops. Featuring contributions from an international group of experts in the field, it provides cutting-edge information on techniques of immense importance to academic, professional and commercial operations. World population is now estimated to be 7.5 billion people, with an annual growth rate of nearly 1.5%. Clearly, the need to enhance not only the quantity of food produced but its quality has never been greater, especially among less developed nations. Genetic manipulation offers the best prospect for achieving that goal. As many fruit crops provide proven health benefits, research efforts need to be focused on improving the nutritional qualities of fruits and vegetables through increased synthesis of lycopene and beta carotene, anthocyanins and some phenolics known to be strong antioxidants. Despite tremendous growth in the area occurring over the past several decades, the work has only just begun. This book represents an effort to address the urgent need to promote those efforts and to mobilise the tools of biotechnical and genetic engineering of the major food crops. Topics covered include: New applications of RNA-interference and virus induced gene silencing (VIGS) for nutritional genomics in crop plants Biotechnological techniques for enhancing carotenoid in crops and their implications for both human health and sustainable development Progress being made in the enrichment and metabolic profiling of diverse carotenoids in a range of fruit crops, including tomatoes, sweet potatoes and tropical fruits Biotechnologies for boosting the phytonutritional values of key crops, including grapes and sweet potatoes Recent progress in the development of transgenic rice engineered to massively accumulate flavonoids in-seed Phytonutritional Improvement of Crops is an important text/reference that belongs in all universities and research establishments where agriculture, horticulture, biological sciences, and food science and technology are studied, taught and applied.
