Overlapping RNA Polymerase Binding Sites Within the Escherichia Coli Lactose Promoter PDF Download
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Author: Martha Lynn Peterson
Publisher:
ISBN:
Category : Escherichia coli
Languages : en
Pages : 242
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Book Description
Author: Martha Lynn Peterson
Publisher:
ISBN:
Category : Escherichia coli
Languages : en
Pages : 242
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Book Description
Author: Donald Duane Lorimer
Publisher:
ISBN:
Category : DNA.
Languages : en
Pages : 260
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Book Description
Author: Jeffrey H. Miller
Publisher:
ISBN:
Category : Science
Languages : en
Pages : 488
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Book Description
Author: Robert W. Li
Publisher: Nova Science Publishers
ISBN: 9781628086447
Category : Microbial ecology
Languages : en
Pages : 0
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Book Description
Metagenomics is the study of microbial communities sampled directly from their natural environment, without prior culturing. DNA sequencing (or alternatively, an assay of functional properties) is applied to the culture-independent analyses of complex and diverse ("meta") populations of microbes. The rapid growth of metagenomics entails an expanded focus on microbes in a wide range of basic and applied research fields, ranging from microbiology to agriculture, biomedicine to bioenergy, and from veterinary to clinical medicine. This book is an insightful and inclusive study on metagenomics and its applications.
Author: Benno Müller-Hill
Publisher: Walter de Gruyter
ISBN: 3110879476
Category : Science
Languages : en
Pages : 221
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Author: Richard J. Noel
Publisher:
ISBN:
Category :
Languages : en
Pages : 302
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Author: Erik Dean Jessen
Publisher:
ISBN:
Category :
Languages : en
Pages : 185
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Book Description
Bacterial transcription, once thought to be organized into discrete, largely non-overlapping units, has been revealed by deep cDNA sequencing to generate ubiquitous, overlapping, sense and antisense RNAs, many of which are noncoding. The function of the extensive antisense transcription of bacterial genes is controversial, and unclear in lieu of mechanistic dissection. We report here characterization of a model antisense transcription unit (bglAS) in the cryptic, H-NS-silenced b-glucoside utilization operon of E. coli K-12 (bglGFBH). bglAS was discovered because inhibition of Rho greatly increased its level and length. We created bglAS- alleles with little or no effect on bglF encoded in the sense strand by disabling the bglAS promoter with base substitutions. Although bglAS exists in a small H-NS-free island in the otherwise H-NS-coated bgl operon, the H-NS distribution is unchanged in bglAS- strains. However, when bglGFBH sense transcription was activated, bglAS decreased b-glucoside-induction of bgl gene expression via BglG-mediated antitermination of the bgl operon attenuators. Using a time series of ChIP-chip, we found that RNA polymerase progression through bglGFBH is hindered by bglAS transcription. In contrast, overexpression of bglAS RNA in trans had no effect on bgl operon induction, supporting bglAS function by transcriptional interference with bglGFBH expression. The bglAS promoter was upregulated by nitrite, consistent with bioinformatic detection of adjacent NarL/P binding sites and suggesting potential bglAS function as an environmental modulator. The proximity of bglAS to the surrounding H-NS filaments led us to investigate the interactions between transcription and H-NS. Consistent with the inability of transcription to affect H-NS binding patterns at the bgl operon, the genome-scale H-NS distribution exhibited minimal change when all transcription was inhibited with rifampicin. However, deletion of H-NS and StpA, an H-NS paralog, resulted in increased progression of RNAP along active, H-NS bound transcription units. Analyzing NET-seq data suggested that H-NS inhibits RNAP progression by promoting pausing of RNAP at non-canonical pause sequences. In contrast to H-NS, the nucleoid-associated protein HU was found to have a distribution pattern mirroring highly active transcription units. Upon further investigation, HU most closely correlated with the presence of R-loops, stable RNA:DNA hybrids external of RNAP. R-loop levels are independent of the presence of HU, suggesting HU is not involved in the resolution of R-loops. Instead, deletion of HU confers sensitivity to reactive oxygen species, and we propose a model by where HU binds to the areas around R-loops to protect DNA from mutagenesis. The combined studies presented here are a significant advancement in our understanding of the function of antisense transcripts and the interaction between transcription and nucleoid-associated proteins.
Author: Stanley Maloy
Publisher: Academic Press
ISBN: 0080961568
Category : Science
Languages : en
Pages : 4360
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Book Description
The explosion of the field of genetics over the last decade, with the new technologies that have stimulated research, suggests that a new sort of reference work is needed to keep pace with such a fast-moving and interdisciplinary field. Brenner's Encyclopedia of Genetics, Second Edition, Seven Volume Set, builds on the foundation of the first edition by addressing many of the key subfields of genetics that were just in their infancy when the first edition was published. The currency and accessibility of this foundational content will be unrivalled, making this work useful for scientists and non-scientists alike. Featuring relatively short entries on genetics topics written by experts in that topic, Brenner's Encyclopedia of Genetics, Second Edition, Seven Volume Set provides an effective way to quickly learn about any aspect of genetics, from Abortive Transduction to Zygotes. Adding to its utility, the work provides short entries that briefly define key terms, and a guide to additional reading and relevant websites for further study. Many of the entries include figures to explain difficult concepts. Key terms in related areas such as biochemistry, cell, and molecular biology are also included, and there are entries that describe historical figures in genetics, providing insights into their careers and discoveries. This 7-volume set represents a 25% expansion from the first edition, with over 1600 articles encompassing this burgeoning field Thoroughly up-to-date, with many new topics and subfields covered that were in their infancy or not inexistence at the time of the first edition. Timely coverage of emergent areas such as epigenetics, personalized genomic medicine, pharmacogenetics, and genetic enhancement technologies Interdisciplinary and global in its outlook, as befits the field of genetics Brief articles, written by experts in the field, which not only discuss, define, and explain key elements of the field, but also provide definition of key terms, suggestions for further reading, and biographical sketches of the key people in the history of genetics
Author: John W. Pelley, PhD
Publisher: Elsevier Health Sciences
ISBN: 0323074464
Category : Medical
Languages : en
Pages : 254
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Book Description
Effectively merge basic science and clinical skills with Elsevier's Integrated Review Biochemistry, by John W. Pelley, PhD. This concise, high-yield title in the popular Integrated Review Series focuses on the core knowledge in biochemistry while linking that information to related concepts from other basic science disciplines. Case-based questions at the end of each chapter enable you to gauge your mastery of the material, and a color-coded format allows you to quickly find the specific guidance you need. Online access via www.studentconsult.com - included with your purchase - allows you to conveniently access the book's complete text and illustrations online as well as relevant content from other Student Consult titles. This concise and user-friendly reference provides crucial guidance for the early years of medical training and USMLE preparation. Spend more time reviewing and less time searching thanks to an extremely focused, "high-yield" presentation. Gauge your mastery of the material and build confidence with both case-based, andUSMLE-style questions that provide effective chapter review and quick practice for your exams. Access the full contents online at www.studentconsult.com where you'll find the complete text and illustrations, "Integration Links" to bonus content in other Student Consult titles, an interactive community center with a wealth of additional resources, and much more! Grasp and retain vital concepts more easily thanks to a color-coded format, succinct,text, key concept boxes, and dynamic illustrations that facilitate learning in a highly visual approach. Effectively review for problem-based courses with the help of text boxes that help you clearly see the clinical relevance of the material. Great for visual learners!
Author: Anne Skaja Robinson
Publisher: John Wiley & Sons
ISBN: 3527634533
Category : Science
Languages : en
Pages : 631
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Book Description
Designed as a research-level guide to current strategies and methods of membrane protein production on the small to intermediate scale, this practice-oriented book provides detailed, step-by-step laboratory protocols as well as an explanation of the principles behind each method, together with a discussion of its relative advantages and disadvantages. Following an introductory section on current challenges in membrane protein production, the book goes on to look at expression systems, emerging methods and approaches, and protein specific considerations. Case studies illustrate how to select or sample the optimal production system for any desired membrane protein, saving both time and money on the laboratory as well as the technical production scale. Unique in its coverage of "difficult" proteins with large membrane-embedded domains, proteins from extremophiles, peripheral membrane proteins, and protein fragments.
