Author: Cheryl Augusta Hawkins
Publisher:
ISBN:
Category :
Languages : en
Pages : 318
Book Description
NMR Characterizations of Sequence-specific, Minor-groove-binding, Polyamide Ligands in Complex with DNA Oligomers
Author: Cheryl Augusta Hawkins
Publisher:
ISBN:
Category :
Languages : en
Pages : 318
Book Description
Publisher:
ISBN:
Category :
Languages : en
Pages : 318
Book Description
Expanding the Heterocyclic Repertoire of DNA Minor Groove Binding Polyamides
Author: Khalid Aman
Publisher:
ISBN:
Category :
Languages : en
Pages : 0
Book Description
Chapter 1 introduces the regulation of gene expression using MGB small molecules.Chapter 2 describes the synthesis of hairpin polyamides, starting with the building blocks and then the solid phase synthetic protocol for constructing 8-ring hairpin polyamides.Chapter 3 showcases the thermal UV melt data, and examines the polyamide binding kinetics with DNA duplexes. Further insight into the binding was obtained with switchSENSE® technology, which allowed the generation of kinetic rate maps. The data outlined in this chapter enabled the profiling of the kinetic parameters that govern PA-dsDNA binding.Chapter 4 provides a structural view of a novel iPr-Im-containing polyamide-DNA complex, highlighting the closer proximity of the N-terminal iPr-Im N2 to the exocyclic amine of G5 compared with the N-terminal iPr-Nt analogue. Furthermore, the 3D NMR-restrained molecular dynamics (MD) structure indicated the major groove compression was greater for polyamide with the terminal iPr-Im monomer compared with the Me-Im analogue, highlighting the importance of a seemingly subtle change in steric bulk facing away from the minor groove for augmenting structural distortion of the DNA duplex.Chapter 5 takes the key findings of this thesis and uses them as a lens for looking to the future direction that this research may take. Namely, the potential of this novel iPr-Im monomer unit to replace imidazole as a G-selective residue that can induce greater structural distortion of the DNA duplex by polyamides.
Publisher:
ISBN:
Category :
Languages : en
Pages : 0
Book Description
Chapter 1 introduces the regulation of gene expression using MGB small molecules.Chapter 2 describes the synthesis of hairpin polyamides, starting with the building blocks and then the solid phase synthetic protocol for constructing 8-ring hairpin polyamides.Chapter 3 showcases the thermal UV melt data, and examines the polyamide binding kinetics with DNA duplexes. Further insight into the binding was obtained with switchSENSE® technology, which allowed the generation of kinetic rate maps. The data outlined in this chapter enabled the profiling of the kinetic parameters that govern PA-dsDNA binding.Chapter 4 provides a structural view of a novel iPr-Im-containing polyamide-DNA complex, highlighting the closer proximity of the N-terminal iPr-Im N2 to the exocyclic amine of G5 compared with the N-terminal iPr-Nt analogue. Furthermore, the 3D NMR-restrained molecular dynamics (MD) structure indicated the major groove compression was greater for polyamide with the terminal iPr-Im monomer compared with the Me-Im analogue, highlighting the importance of a seemingly subtle change in steric bulk facing away from the minor groove for augmenting structural distortion of the DNA duplex.Chapter 5 takes the key findings of this thesis and uses them as a lens for looking to the future direction that this research may take. Namely, the potential of this novel iPr-Im monomer unit to replace imidazole as a G-selective residue that can induce greater structural distortion of the DNA duplex by polyamides.
Sequence-specific Minor Groove Binding Polyamides
Author: Scott Reynolds Carter
Publisher:
ISBN:
Category : Electronic dissertations
Languages : en
Pages : 396
Book Description
Publisher:
ISBN:
Category : Electronic dissertations
Languages : en
Pages : 396
Book Description
Dissertation Abstracts International
Author:
Publisher:
ISBN:
Category : Dissertations, Academic
Languages : en
Pages : 656
Book Description
Publisher:
ISBN:
Category : Dissertations, Academic
Languages : en
Pages : 656
Book Description
American Doctoral Dissertations
Author:
Publisher:
ISBN:
Category : Dissertation abstracts
Languages : en
Pages : 776
Book Description
Publisher:
ISBN:
Category : Dissertation abstracts
Languages : en
Pages : 776
Book Description
Chemical Abstracts
Author:
Publisher:
ISBN:
Category : Chemistry
Languages : en
Pages : 2668
Book Description
Publisher:
ISBN:
Category : Chemistry
Languages : en
Pages : 2668
Book Description
Cumulated Index Medicus
Author:
Publisher:
ISBN:
Category : Medicine
Languages : en
Pages : 1498
Book Description
Publisher:
ISBN:
Category : Medicine
Languages : en
Pages : 1498
Book Description
Proceedings of the National Academy of Sciences of the United States of America
Author: National Academy of Sciences (U.S.)
Publisher:
ISBN:
Category : Science
Languages : en
Pages : 1404
Book Description
Publisher:
ISBN:
Category : Science
Languages : en
Pages : 1404
Book Description
Nucleic Acids
Author: Victor A. Bloomfield
Publisher: Sterling Publishing Company
ISBN: 9780935702491
Category : Science
Languages : en
Pages : 854
Book Description
Providing a comprehensive account of the structures and physical chemistry properties of nucleic acids, with special emphasis on biological function, this text has been organized to meet the needs of those who have only a basic understanding of physical chemistry and molecular biology.
Publisher: Sterling Publishing Company
ISBN: 9780935702491
Category : Science
Languages : en
Pages : 854
Book Description
Providing a comprehensive account of the structures and physical chemistry properties of nucleic acids, with special emphasis on biological function, this text has been organized to meet the needs of those who have only a basic understanding of physical chemistry and molecular biology.
Anion Receptor Chemistry
Author: Jonathan L Sessler
Publisher: Royal Society of Chemistry
ISBN: 1847552471
Category : Science
Languages : en
Pages : 431
Book Description
Anion recognition plays a critical role in a range of biological processes, and a variety of receptors and carriers can be found throughout the natural world. Chemists working in the area of supramolecular chemistry have created a range of anion receptors, drawing inspiration from nature as well as their own inventive processes. This book traces the origins of anion recognition chemistry as a unique sub-field in supramolecular chemistry while illustrating the basic approaches currently being used to effect receptor design. The combination of biological overview and summary of current synthetic approaches provides a coverage that is both comprehensive and comprehensible. First, the authors detail the key design motifs that have been used to generate synthetic receptors and which are likely to provide the basis for further developments. They also highlight briefly some of the features that are present in naturally occurring anion recognition and transport systems and summarise the applications of anion recognition chemistry. Providing as it does a detailed review for practitioners in the field and a concise introduction to the topic for newcomers, Anion Receptor Chemistry reflects the current state of the art. Fully referenced and illustrated in colour, it is a welcome addition to the literature.
Publisher: Royal Society of Chemistry
ISBN: 1847552471
Category : Science
Languages : en
Pages : 431
Book Description
Anion recognition plays a critical role in a range of biological processes, and a variety of receptors and carriers can be found throughout the natural world. Chemists working in the area of supramolecular chemistry have created a range of anion receptors, drawing inspiration from nature as well as their own inventive processes. This book traces the origins of anion recognition chemistry as a unique sub-field in supramolecular chemistry while illustrating the basic approaches currently being used to effect receptor design. The combination of biological overview and summary of current synthetic approaches provides a coverage that is both comprehensive and comprehensible. First, the authors detail the key design motifs that have been used to generate synthetic receptors and which are likely to provide the basis for further developments. They also highlight briefly some of the features that are present in naturally occurring anion recognition and transport systems and summarise the applications of anion recognition chemistry. Providing as it does a detailed review for practitioners in the field and a concise introduction to the topic for newcomers, Anion Receptor Chemistry reflects the current state of the art. Fully referenced and illustrated in colour, it is a welcome addition to the literature.