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Author: Onno Bakker
Publisher: Springer Science & Business Media
ISBN: 3662084317
Category : Science
Languages : en
Pages : 57
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Book Description
The instrument Lumi-Imager was developed for the detection and accurate quantification of chemiluminescent signals on blots and in microtiter plates. It contains a cooled CCD camera, a specially developed lens system and software. In addition to luminescence, Lumi-Imager F1 can also detect and quantify UV-excited fluorescent signals in gels. Here we describe the components and features of this instrument as well as some of its applications.
Author: Onno Bakker
Publisher: Springer Science & Business Media
ISBN: 3662084317
Category : Science
Languages : en
Pages : 57
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Book Description
The instrument Lumi-Imager was developed for the detection and accurate quantification of chemiluminescent signals on blots and in microtiter plates. It contains a cooled CCD camera, a specially developed lens system and software. In addition to luminescence, Lumi-Imager F1 can also detect and quantify UV-excited fluorescent signals in gels. Here we describe the components and features of this instrument as well as some of its applications.
Author: Daniel Klionsky
Publisher: Academic Press
ISBN: 0080923259
Category : Science
Languages : en
Pages : 571
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Book Description
This is the companion volume to Daniel Klionsky's Autophagy: Lower Eukaryotes, which features the basic methods in autophagy covering yeasts and alternative fungi (aspergillus, podospora, magnaporthe). Klionsky is one of the leading authorities in the field. He is the editor-in-chief of Autophagy. The November 2007 issue of Nature Reviews highlighted his article, "Autophagy: From phenomenology to molecular understanding in less than a decade. He is currently editing guidelines for the field, with 230 contributing authors, that will publish in Autophagy.Particularly in times of stress, like starvation and disease, higher organisms have an internal mechanism in their cells for chewing up and recycling parts of themselves. The process of internal "house cleaning in the cell is called autophagy – literally self-eating. Breakthroughs in understanding the molecular basis of autophagy came after the cloning of ATG1 (autophagy-related gene 1) in yeast. (To date, 30 additional yeast genes have been identified.) These ATG genes in yeast were the stepping stones to the explosion of research into the molecular analysis of autophagy in higher eukaryotes. In the future, this research will help to design clinical approaches that can turn on autophagy and halt tumor growth.
Author: Takatoki Yamamoto
Publisher: Frontiers Media SA
ISBN: 2889452158
Category :
Languages : en
Pages : 193
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Book Description
Infectious diseases are associated with approximately 20% of global mortality, with viral diseases causing about one third of these deaths. Besides newly emerging and re-emerging viral infections will continue to pose a threat to human survival globally. In this case scientific advances have greatly been increased to defend against those pathogens. For example, rapid genomic sequencing, proteomics, epigenomics, nanotechnology, and other advanced tools are being applied to detect viruses at the point of care and to track their spread within human populations as well as to understand virus-host interaction and virus induced pathogenesis. From rapid identification of new viruses to prevention with vaccination and treatment with effective therapeutics, biomedical research has continuously provided tools to meet the constant threat of emerging viral pathogens. Despite these advances, each new disease brings unique challenges to scientists every year. So we must stay at the cutting edge of scientific discovery, working energetically to develop new tools to combat the ever-changing threats they pose. Our research topic highlights such advanced and new technology based virus research which definitely bolsters the researcher's ability to tackle emerging, re-emerging and stable viral pathogens. We are credulous that the papers including in the e-books will be beneficial to the experts in the field to understand the molecular, immunological, ecological and clinical aspects of the next generation researches for the prevention and control of infectious diseases caused by viruses.
Author: John M. Walker
Publisher: Springer
ISBN: 1592591698
Category : Science
Languages : en
Pages : 1099
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Book Description
The Protein Protocols Handbook, Second Edition aims to provide a cross-section of analytical techniques commonly used for proteins and peptides, thus providing a benchtop manual and guide for those who are new to the protein chemistry laboratory and for those more established workers who wish to use a technique for the first time. All chapters are written in the same format as that used in the Methods in Molecular BiologyTM series. Each chapter opens with a description of the basic theory behind the method being described. The Materials section lists all the chemicals, reagents, buffers, and other materials necessary for carrying out the protocol. Since the principal goal of the book is to provide experimentalists with a full account of the practical steps necessary for carrying out each protocol successfully, the Methods section contains detailed st- by-step descriptions of every protocol that should result in the successful execution of each method. The Notes section complements the Methods material by indicating how best to deal with any problem or difficulty that may arise when using a given technique, and how to go about making the widest variety of modifications or alterations to the protocol. Since the first edition of this book was published in 1996 there have, of course, been significant developments in the field of protein chemistry.
Author: Nejat Duzgunes
Publisher: Elsevier
ISBN: 008049711X
Category : Science
Languages : en
Pages : 517
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Book Description
Liposomes are cellular structures made up of lipid molecules. Important as a cellular model in the study of basic biology, liposomes are also used in clinical applications such as drug delivery and virus studies. - Liposomes in Biochemistry - Liposomes in Molecular Cell Biology - Liposomes in Molecular Virology
Author: D Scott Merrell
Publisher: Frontiers E-books
ISBN: 2889191109
Category :
Languages : en
Pages : 328
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Book Description
Campylobacter spp and Helicobacter spp are gastrointestinal pathogens that remain a major cause of acute gastroenteritis and gastric disease, respectively. The 16th International Workshop on Campylobacter, Helicobacter and Related Organisms (CHRO) will be held in Vancouver, British Columbia Canada from August 28-September 1, 2011 and will highlight recent advances in our understanding of the epidemiology, survival mechanisms, host response and pathogenesis of these important species. This Research Topic issue will highlight each of these topics and will attempt to shed insight into our growing understanding of the process of host-pathogen interactions as it relates to Campylobacter and Helicobacter.
Author: John Michels (Journalist)
Publisher:
ISBN:
Category : Science
Languages : en
Pages : 998
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Book Description
Author: Michael Fromm
Publisher: John Wiley & Sons
ISBN: 1573317497
Category : Medical
Languages : en
Pages : 357
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Book Description
Aim of this volume is to clarify the relationship between molecular structure and function of tight junction proteins, as well as their regulation and their role in diseases. Current research may form a basis for future diagnostic and therapeutic approaches to diseases which seem to have not much in common but are characterized by defects of organ barriers, like Crohn's disease, renal hypertension, inner ear deafness, and cancerous diseases. Topics include the functions of distinct tight junction proteins as barrier or channel formers for solutes and water, characteristics of the tight junction in inflammatory bowel diseases, posttranslational modifications of tight junction proteins, the relation between renal tight junction proteins and blood pressure control, and the molecular structure of claudin-claudin interactions NOTE: Annals volumes are available for sale as individual books or as a journal. For information on institutional journal subscriptions, please visit www.blackwellpublishing.com/nyas. ACADEMY MEMBERS: Please contact the New York Academy of Sciences directly to place your order (www.nyas.org). Members of the New York Academy of Science receive full-text access to the Annals online and discounts on print volumes. Please visit http://www.nyas.org/MemberCenter/Join.aspx for more information about becoming a member.
Author: Michael R. Kreutz
Publisher: Frontiers Media SA
ISBN: 2889450031
Category :
Languages : en
Pages : 214
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Book Description
Ca2+ signaling in neurons is characterized by highly restricted and dynamic gradients called Ca2+ waves, spikes, transients and puffs depending upon their corresponding spatial and temporal features. Based on this strict segmentation the Ca2+ ion provides a versatile basis for complex signaling in neuronal subcompartments with a spatial resolution of micro- and nanodomains. The multitude of Ca2+-regulated processes requires specialized downstream processing machinery, translating the Ca2+ signal into alterations of cellular processes. The broad range of different Ca2+-triggered phenomena in neurons, ranging from neurotransmission to gene expression, is reflected by the existence of a multitude of different Ca2+-binding proteins (CaBPs) from which numerous belong to the EF-hand super-family. EF-hand proteins can be subdivided into Ca2+ buffer and Ca2+ sensor proteins. Whereas the first group has a very high affinity for Ca2+, exhibits little conformational change in the Ca2+-bound state and is thought to mainly chelate Ca2+, the second group has a lower affinity for Ca2+ and shows considerable conformational changes upon Ca2+-binding, which usually triggers a target interaction. Neuronal calcium sensor (NCS) proteins and the related Caldendrin/CaBP/Calneuron (nCaBPs) proteins are members of this latter group. They resemble the structure of their common ancestor Calmodulin (CaM) with four EF-hand Ca2+-binding motifs, of which not all are functional. However, despite their structural homology with CaM, NCS as well as nCaBPs are quite diverse in amino acid sequence. It is therefore surprising that relatively few binding partners have been identified that are not CaM targets and this raises the question of the specificity and function of these interactions. In terms of function, binding of NCS and nCaBP has frequently different consequences than binding of CaM, which substantially increases the versatility of the Ca2+ tool kit. The general idea of this special issue is to provide an overview on the function of neuronal EF-hand calcium-binding proteins in health and disease. But we will not just provide a mere collection of articles to stress the function of each protein. The issue will mainly deal with emerging concepts on Ca2+-signaling/buffering mediated by EF-hand Ca2+-binding proteins. This includes questions like features that define the functional role of a EF-hand calcium sensor in neurons, the conditions that make physiological relevance of a given interaction of a CaBP with its target plausible, the emerging synaptic role of these proteins, and mounting evidence for their role in the regulation of protein trafficking. Structural aspects and biophysical studies will be covered. Another aspect will be the role of CaBPs in brain disease states. This aspect includes studies showing that CaBPs are targets of drugs in clinical use, studies showing that expression levels of calcium-binding proteins are frequently altered in brain disease states as well as reports on mutations in EF-hand calcium sensors linked to human disease.
Author:
Publisher: Elsevier
ISBN: 0080496679
Category : Science
Languages : en
Pages : 861
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Book Description
This volume includes a core of methodologies to attack the unique experimental problems presented by protein misassembly. Emphasis is on human biology applications, the area in which there is the most interest, in which most of the work has already been done, and in which there is the best evidence for the structural sophisitication of the protein aggregates.The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
