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Author:
Publisher: Academic Press
ISBN: 0128208112
Category : Science
Languages : en
Pages : 414
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Book Description
Immunological Methods in Microbiology, Volume 47 in the Methods in Microbiology series, highlights new advances in the field, with this new volume presenting interesting chapters on Immunological Techniques in the Clinical laboratory, Immunologic Diagnosis of HIV and Opportunistic Infections, Combining Antigen Detection and Serology for the Diagnosis of Selected Infectious Diseases, Immunologic Detection of Lyme Disease and Related Borrelioses, Immunodetection of Bacteria Causing Brucellosis, Immunological Diagnostic Techniques Used to Identify and Type Pasteurella, Immunological Tests for Diarrhea caused by Diarrheagenic Escherichia coli Targeting Their Main Virulence Factors, and much more. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Microbiology series Includes the latest information on Immunological Methods in Microbiology
Author:
Publisher: Academic Press
ISBN: 0128208112
Category : Science
Languages : en
Pages : 414
Get Book Here
Book Description
Immunological Methods in Microbiology, Volume 47 in the Methods in Microbiology series, highlights new advances in the field, with this new volume presenting interesting chapters on Immunological Techniques in the Clinical laboratory, Immunologic Diagnosis of HIV and Opportunistic Infections, Combining Antigen Detection and Serology for the Diagnosis of Selected Infectious Diseases, Immunologic Detection of Lyme Disease and Related Borrelioses, Immunodetection of Bacteria Causing Brucellosis, Immunological Diagnostic Techniques Used to Identify and Type Pasteurella, Immunological Tests for Diarrhea caused by Diarrheagenic Escherichia coli Targeting Their Main Virulence Factors, and much more. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Microbiology series Includes the latest information on Immunological Methods in Microbiology
Author: John M. Grange
Publisher: Wiley-Blackwell
ISBN: 9780632019083
Category : Antibodies, Monoclonal
Languages : en
Pages : 271
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Book Description
In recent years there have been many technical developments in immunology that have potential or actual applications in many other branches of the life sciences. These developments include a number of ultrasensitive systems for the detection and assay of a wide range of biological materials, including micro-organisms and their products, as well as techniques for isolating and purifying micro-organisms and other antigenic materials. Two developments in particular have greatly extended the scope and applicability of immunological methods. These are the production of monoclonal antibodies and the introduction of enzyme linked immunoassay as a simple, safe and economical alternative to radioimmunoassay. This book is based on information gathered at the Society for Applied Bacteriology Autumn Demonstration Meeting held at the South Bank Polytechnic, London on October 15th, 1986.
Author: Antti Vaheri
Publisher: Springer Science & Business Media
ISBN: 364276603X
Category : Medical
Languages : en
Pages : 585
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Book Description
The current diagnostic methods for the great variety of microbial agents affecting health are clearly unsatisfactory. New important pathogens have emerged including the agent responsible for bovine spongiform encephalopathy. Moreover, there is an increasing need for more accurate microbial control of our environment, and of the food and water we consume. What is needed are rapid, sensitive and reliable procedures which, on the one hand, should be suitable for automation and, onthe other hand, presented in a cost-effective version suitable for field use. Including new biochemical approaches, such as polymerase chain reaction, recombinant gene products and synthetic peptides, these needs are discussed in these protocols of the RAMI-90 congress.
Author: K.-O. Habermehl
Publisher: Springer Science & Business Media
ISBN: 364269943X
Category : Medical
Languages : en
Pages : 745
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Book Description
Rapid progress in molecular biology, genetic engineering, and basic research in immunology has opened up new possibilities for application to diagnostic procedures and to clinical research. In a short period a new era of diagnosis dawned, covering nearly all fields of microbiology, immunology, and food technology. In consequence of this rapid development, scientists of many disciplines are involved studying infections of humans, animals, and plants or working in technical microbiology. The application of the newest findings of basic research to diagnostic work and to clinical research covers nearly all fields of microbiology and immunology. Moreover, it underlines the close relationship between diagnosis, therapy, and epidemiology. An outstanding example of these connections is given by the recent development of hepatitis B vaccine. The discovery and identification of a non cultivable agent by physicochemical and immunological methods were the heralds of a new era in the prevention of infectious diseases. This book provides an up-to-date, comprehensive review of developments and future aspects in various fields. I am convinced that the authors have succeeded in furnishing a large variety of new ideas and possibilities. K.-O. HABERMEHL Contents Time Realities in the Evaluation of Vaccines for Safety and Efficacy The Evaluation of Vaccines M. R. HILLEMAN . . . . .
Author: Ivan Lefkovits
Publisher: Academic Press
ISBN: 148326999X
Category : Science
Languages : en
Pages : 492
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Book Description
Immunological Methods a compendium of basic research techniques being used in one of the largest immunology research institutes, the Basel Institute for Immunology, with particular emphasis given to new methodology. The procedures have been described by individuals judged to be highly expert in their specialties. In many instances the methods developed or adapted to unique uses by the contributors have not previously been described in detail. The book contains 34 chapters covering techniques for detection, isolation, and purification of antibodies (including dansylation, two-dimensional chromatography, isoelectric focusing, polyacrylamide gel electrophoresis, and isotachophoresis); measurement of equilibrium constants (equilibrium dialysis, filtration, and sedimentation); and isotope and fluorescent labeling and detection of cell-surface components. Techniques such as isotope laboratory maintenance; chemical modification of proteins, haptens, and solid supports, and haptenation of viable biological carriers; production of antisera against allotypes and histocompatibility antigens and production of antibody with clonai dominance; histocompatibility and MLR testing; and cell separation by haptenated gels and by velocity sedimentation of rosette-forming cells are also discussed. Other chapters cover detection of antibody-secreting and alloantigen-binding cells; immune responses in vitro and their analysis by limiting dilution; production of T-cell factors; hybridoma production by cell fusion; maintenance of cell lines and cloning in semisolid media; and the mathematical analysis of immunological data.
Author: Wendy J. Palmer
Publisher: IRL Press
ISBN:
Category : Medical
Languages : en
Pages : 282
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Book Description
2978 entries to international journal literature published between 1967-1975. References derived from about 450 source journals. Classified arrangement. Entry gives title, all authors, bibliographic information, and address of primary author. Author, subject indexes.
Author: Elmer S. Krudy
Publisher: IRL Press
ISBN:
Category : Medical
Languages : en
Pages : 284
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Book Description
Author: National Research Council
Publisher: National Academies Press
ISBN: 0309091225
Category : Nature
Languages : en
Pages : 329
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Book Description
Recent and forecasted advances in microbiology, molecular biology, and analytical chemistry have made it timely to reassess the current paradigm of relying predominantly or exclusively on traditional bacterial indicators for all types of waterborne pathogens. Nonetheless, indicator approaches will still be required for the foreseeable future because it is not practical or feasible to monitor for the complete spectrum of microorganisms that may occur in water, and many known pathogens are difficult to detect directly and reliably in water samples. This comprehensive report recommends the development and use of a "tool box" approach by the U.S Environmental Protection Agency and others for assessing microbial water quality in which available indicator organisms (and/or pathogens in some cases) and detection method(s) are matched to the requirements of a particular application. The report further recommends the use of a phased, three-level monitoring framework to support the selection of indicators and indicator approaches.Â
Author: Heddy Zola
Publisher: CRC Press
ISBN:
Category : Medical
Languages : en
Pages : 288
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Book Description
This two-volume reference details immunological techniques for biologists of all disciplines. Volume I includes a detailed discussion of the tissue culture laboratory. It addresses what the lab needs to be, and the general "housekeeping" procedures involved in tissue culture. Presented next are chapters on specific aspects of tissue culture and hybridoma technology. The book includes a review of bioassays for interleukins, and a series of papers on lymphokines and functional assays in vitro. The section on molecular genetic studies begins with consideration of the choice of strategies for cloning the genes of cell surface molecules. It continues with papers on aspects of molecular biological techniques most closely related to immunology. The final section covers immunochemical techniques. Volume II reviews techniques used with small laboratory animals. It includes papers on specialized procedures with animals. Technical aspects are emphasized through a detailed analysis of effects of ultraviolet light on the immune system. Covered also is antigen detection in cells and tissue. The book addresses the important areas of protein purification using monoclonal antibodies.These two volumes are of great importance to those who use immunological techniques, whether they are immunologists or trained in other disciplines. The book is intended for those in animal science, veterinary science, genetics and cell biology, bacteriology, immunology, pathology, biochemistry, laboratory medicine, and hematology.
Author: Ajit Varma
Publisher: Springer Science & Business Media
ISBN: 3540708650
Category : Science
Languages : en
Pages : 438
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Book Description
This book presents a wide range of biotechnological methods for application in soil microbiology analysis, including all essential methods involving molecular biology, immunology, microbiology, and structural biology, such as transcriptome analysis, RNAi technology, molecular matchmaking, RAPD, T-RFLP and FT/MS. The techniques and procedures presented here offer practical guides for immediate use in the laboratory. This volume will be of use both to the first-timer and to the experienced scientist.