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Author: S.C. Makrides
Publisher: Newnes
ISBN: 0080930352
Category : Science
Languages : en
Pages : 776
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Book Description
The production of proteins in mammalian cells is an important tool in numerous scientific and commercial areas. For example, proteins for human therapy, vaccination or diagnostic applications are typically produced in mammalian cells. Gene cloning, protein engineering, biochemical and biophysical characterization of proteins also require the use of gene expression in mammalian cells. Other applications in widespread use involve screening of libraries of chemical compounds in drug discovery, and the development of cell-based biosensors.This book presents a state-of-the-art comprehensive coverage of the technical aspects of gene expression in mammalian cells, written by experienced scientists working at the forefront of the field.
Author: S.C. Makrides
Publisher: Newnes
ISBN: 0080930352
Category : Science
Languages : en
Pages : 776
Get Book Here
Book Description
The production of proteins in mammalian cells is an important tool in numerous scientific and commercial areas. For example, proteins for human therapy, vaccination or diagnostic applications are typically produced in mammalian cells. Gene cloning, protein engineering, biochemical and biophysical characterization of proteins also require the use of gene expression in mammalian cells. Other applications in widespread use involve screening of libraries of chemical compounds in drug discovery, and the development of cell-based biosensors.This book presents a state-of-the-art comprehensive coverage of the technical aspects of gene expression in mammalian cells, written by experienced scientists working at the forefront of the field.
Author: Jeffrey H. Miller
Publisher:
ISBN:
Category : Science
Languages : en
Pages : 188
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Book Description
Author: William C. Heiser
Publisher: Springer Science & Business Media
ISBN: 1592596495
Category : Science
Languages : en
Pages : 303
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Book Description
Experienced researchers describe in step-by-step detail methods that have proven most useful in delivering genes to mammalian cells. Volume 1 focuses on gene delivery by a variety of chemical and physical methods, including ultrasound, biolistics, peptides, PNA clamps, liposomes, microinjection, electroporation, particle bombardment, dendrimers, and hydrodynamics. Volume 2 details procedures for delivering genes to cells in vitro and in vivo, including the use of lentiviral vectors.
Author: Michael Kriegler
Publisher: Oxford University Press, USA
ISBN: 9780716770046
Category : Gene Expression Regulation
Languages : en
Pages : 242
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Book Description
This book presents a descriptive, comprehensive catalogue of viral and cellular cis-acting elements and the latest expression vectors for obtaining optimum expression of foreign genes introduced into mammalian cells. Included is a selection of optimized, state-of-the-art protocols for transferring genes into mammalian cells and subsequently assaying for transfer and expression of the genes of interest. This unique volume describes in detail essential techniques for propagating cells and cell lines, DNA transfer techniques, drug selection and gene amplification techniques, expression cloning, subtractive hybridization and retrovirus-mediated gene transfer techniques. Also featured are highly sensitive assays for gene transfer and expression, including detection of DNA and RNA by both isotopic and non-isotopic detection methods as well as the most sensitive non-isotopic Western techniques for the detection of proteins.
Author: Edward J. Murray
Publisher: Springer Science & Business Media
ISBN: 1592594948
Category : Medical
Languages : en
Pages : 417
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Book Description
Biology is the study of living things. The classical approach might be described as holistic and descriptive, whereas the modern molecular - proach aims to be investigative, reductionist, and mechanistic . Genes contain all the information for the structure of all living things ; thus, the understanding of how genes are regulated is an important step toward understanding the nature of living things. The study of gene regulation has been made more tractable by the design of simple expe- mental models in which a single gene can be isolated from the milieu of the organism. The new science of molecular biology has introduced techniques that permit the design of such experimental models. In - sence, the genome of the organism is dissected in such a manner that specific genes may now be introduced into an appropriate cell line . Subsequent analysis of the proteins expressed from the genes under study results in the identification of the regulatory DNA sequences .
Author: Richard Twyman
Publisher: Taylor & Francis
ISBN: 1135322791
Category : Science
Languages : en
Pages : 299
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Book Description
Gene transfer to animal cells was first achieved more than thirty years ago. Since then, transformation technology has developed rapidly, resulting in a multitude of techniques for cell transformation and the creation of transgenic animals. As with any expanding technology, it becomes difficult to keep track of all the developments and to find a concise and comprehensive source of information that explains all the underlying principles. Gene Transfer to Animals Cells addresses this problem by describing the principles behind gene transfer technologies, how gene expression is controlled in animal cells and how advanced strategies can be used to add, exchange or delete sequences from animal genomes in a conditional manner. A final chapter provides an overview of all the applications of animal cell transformation in farming, medicine and research.
Author:
Publisher:
ISBN:
Category : Biochemistry
Languages : en
Pages : 680
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Book Description
Author: R. Kucherlapati
Publisher: Springer Science & Business Media
ISBN: 1468451677
Category : Medical
Languages : en
Pages : 447
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Book Description
Genetic analysis of microbial systems provided us with the foundation for un derstanding gene structure, expression, and regulation. It was long felt that the ability to generate mutants and conduct genetic studies in mammalian systems would prove to be equally useful. However, genetic analysis based on sexual systems is difficult in mammals because of the long generation times and the inability to perform controlled matings. As a result, genetic analysis of mam malian systems had to await the development of parasexual systems. This book is an attempt to bring together descriptions of a number of these parasexual systems. A common theme of all the parasexual systems is the transfer of genetic information from a defined source into a specific cell type. This volume deals with a number of methods of gene transfer into mammalian cells. The early methods of gene transfer involved transfer of relatively large amounts of genetic information. These include somatic cell hybridization, microcell fusion, and chromosome transfer, which constitute the first part of this book. Each of these methods has already proven to be of enormous value in arriving at a genetic understanding of the mammalian genome. Development of recombinant DNA methods, and the ability to introduce purified DNA into mammalian cells, has had a significant impact on our ability to dissect important aspects of mammalian gene expression and regulation. The second part of this book deals with gene transfer systems involving defined nucleic acid sequences.
Author: Theodore Friedmann
Publisher: CSHL Press
ISBN: 0879697652
Category : Gene Transfer Techniques
Languages : en
Pages : 769
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Book Description
Understanding gene function and regulation requires rigorous testing in live cells and organisms. Recent advances have provided a variety of new strategies for delivering DNA and RNA into cells and probing their expression, as well as new clinical applications that rely upon the introduction of genetic material. The vast number of available techniques for clinical and laboratory research often makes selecting the optimal method a difficult process. Gene Transfer: Delivery and Expression of DNA and RNA provides the first comprehensive guide to technical approaches for delivering nucleic acids into cells and organisms and of ensuring (even manipulating) appropriate expression. The detailed, step-by-step protocols cover a variety of methods, both well established and newly evolving. These include viral and nonviral methods of gene delivery, transgenic approaches, strategies for the regulation of transgene expression, and modification of the host response. The introductory matter to each chapter includes concise technical and theoretical discussions with considerations for selection of the appropriate system and strategies for delivery.
Author: S.C. Makrides
Publisher: Gulf Professional Publishing
ISBN: 9780444513700
Category : Science
Languages : en
Pages : 730
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Book Description
This volume provides a broad, state-of-the-art coverage of diverse technical topics in gene expression in mammalian cells, including the development of vectors for production of proteins in cultured cells, in transgenic animals, vaccination, and gene therapy; progress in methods for the transfer of genes into mammalian cells and the optimization and monitoring of gene expression; advances in our understanding and manipulation of cellular biochemical pathways that have a quantitative and qualitative impact on mammalian gene expression; and the large-scale production and purification of proteins from cultured cells.
