Functional and Structural Characterization of Bacterial Outer Membrane Proteins PDF Download
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Author: Victoria Hritonenko
Publisher:
ISBN:
Category : Bacterial cell walls
Languages : en
Pages : 296
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Book Description
Author: Victoria Hritonenko
Publisher:
ISBN:
Category : Bacterial cell walls
Languages : en
Pages : 296
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Book Description
Author:
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Author: Giulia Tamburrino
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Author: Robert Arjen Kramer
Publisher:
ISBN: 9789039327913
Category :
Languages : en
Pages : 144
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Book Description
Author: Jean-Jacques Lacapere
Publisher: Humana Press
ISBN: 9781493971497
Category : Science
Languages : en
Pages : 419
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Book Description
In this present volume, different approaches are detailed to produce membrane proteins, purify them, study their function, determine their structure, and model them in membrane. Since every membrane protein behaves mostly in a unique way /fashion, knowledge of guidelines and tricks may help to increase chances to express, purify and characterize a peculiar membrane protein. Production of correctly folded protein remains a challenge. Moreover, getting a functional and stable protein requires to optimize membrane mimicking environments that can be detergent or artificial membranes. In some cases, the finding of the correct ligand which will stabilize the desired conformation is needed. In other cases, stabilization can be obtained using specific antibodies. This volume also presents different techniques to analyze the functional status of membrane proteins. Written in the highly successful Methods in Molecular Biology series format, chapters in Membrane Protein Structure and Function Characterization: Methods and Protocols provide different techniques to analyze the functional and structural status of membrane proteins. Chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Membrane Protein Structure and Function Characterization: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Author: Carey Anne Hobbs
Publisher:
ISBN:
Category :
Languages : en
Pages : 213
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Book Description
Keywords: Competence, NMR, Calbindin, response regulator, mass spectrometry, differential surface modification.
![Structural Analysis of the Escherichia Coli [beta]-barrel Assembly Machinery Complex PDF](https://books.google.com/books/content/images/frontcover/HZN-0AEACAAJ?fife=w80-h100)
Author: Kelly (Hyojin) Kim
Publisher:
ISBN:
Category : Escherichia coli
Languages : en
Pages : 0
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Book Description
The outer membrane (OM) is a unique structural feature of Gram-negative bacteria. Residing within the outer membrane are [beta]-barrel outer membrane proteins (OMPs) that serve many important cellular functions. As proper folding and assembly of these proteins are crucial for cell viability, Gram-negative bacteria possess a specialized proteinaceous machine, known as the BAM ([beta]-barrel assembly machinery) complex, to catalyze the folding and membrane insertion of OMPs. In Escherichia coli, the BAM complex consists of five proteins: one [beta]-barrel membrane protein -- BamA, and four lipoproteins -- BamB, BamC, BamD, and BamE. The roles of the individual components and how they are arranged into the BAM complex to function together is not yet clearly understood. During the course of this thesis project, I determined the structures of E. coli BamB, BamC, BamE and the BamCD subcomplex. Analysis of the conserved residues and the molecular surface properties of these solved structures helped to identify potential protein-protein interaction sites on each lipoprotein. For example, BamC has two 'helix-grip' domains that are ideally shaped to accommodate [alpha]-helices. BamB, on the other hand, has a [beta]-propeller fold that could potentially interact with BamA or substrates via [beta]-augmentation, a mode of interaction in which a pre-existing [beta]-sheet is augmented by an addition of a [beta]-strand of another protein. Comparing the solved structures with their structural homologs with known functions has also provided important clues about the functional roles of each lipoprotein. BamD structure, for example, closely resembles the binding pocket of a peroxisomal targeting signal receptor PEX5, suggesting a similar substrate recognition function for BamD. Interestingly, our BamCD complex structure shows that the putative substrate binding pocket of BamD is bound and blocked by the conserved unstructured N-terminal region of BamC. This suggests a possibility that BamC may have a regulatory function. The structural and interaction data acquired from this thesis project contributes to a better understanding of the BAM complex structure and provides a platform for future research driven by structure-based hypotheses.
Author: Susan Buchanan
Publisher: Humana
ISBN: 9781493928705
Category : Science
Languages : en
Pages : 0
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Book Description
This volume is comprised of a collection of experimental protocols for common techniques and strategies used to study the biogenesis of b-barrel outer membrane proteins in Gram-negative bacteria. The BAM Complex: Methods and Protocols guides readers through methods on the function of the BAM complex, the roles played by each of the individual components, the expression and purification of the components, crystallization and structure determination of the components, and how the individual Bam components may assemble into a functional complex. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, The BAM Complex: Methods and Protocols will serve as an invaluable reference for those interested in studying the BAM complex.
Author: Bruce Alberts
Publisher:
ISBN: 9780815332183
Category : Cytology
Languages : en
Pages : 0
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Book Description
Author: J.-M. Ghuysen
Publisher: Elsevier
ISBN: 0080860877
Category : Science
Languages : en
Pages : 607
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Book Description
Studies of the bacterial cell wall emerged as a new field of research in the early 1950s, and has flourished in a multitude of directions. This excellent book provides an integrated collection of contributions forming a fundamental reference for researchers and of general use to teachers, advanced students in the life sciences, and all scientists in bacterial cell wall research. Chapters include topics such as: Peptidoglycan, an essential constituent of bacterial endospores; Teichoic and teichuronic acids, lipoteichoic acids, lipoglycans, neural complex polysaccharides and several specialized proteins are frequently unique wall-associated components of Gram-positive bacteria; Bacterial cells evolving signal transduction pathways; Underlying mechanisms of bacterial resistance to antibiotics.
