Fluorescence Studies on Biological Membranes

Fluorescence Studies on Biological Membranes PDF Author: Herwig J. Hilderson
Publisher: Springer Science & Business Media
ISBN: 1461393590
Category : Science
Languages : en
Pages : 480

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Book Description
As stated by its first editor, Dr. D. B. Roodyn, the primary goal of the series Subcellular Biochemistry is to achieve an integrated view of the cell by bringing together results from a wide range of different techniques and disciplines. This volume deals with the applications of fluorescence spectroscopy to membrane research. It seeks to present complementary biochemical and bio physical data on both the structure and the dynamics of biological membranes. Biophysics and biochemistry are improving more and more in their ability to study biomembranes, overlapping somewhat in this area and explaining the functioning of the whole cell in terms of the properties of its individual com ponents. Therefore, we have brought together an international group of experts in order to report on and review advances in fluorescence studies on biological membranes, thereby highlighting subcellular aspects. The first chapters present a critical evaluation of the current applications of dynamic and steady-state fluorescence techniques. Subsequent chapters dis cuss more specific applications in cells, biological membranes, and their con stituents (lipids, proteins).

Fluorescence Studies on Biological Membranes

Fluorescence Studies on Biological Membranes PDF Author: Herwig J. Hilderson
Publisher: Springer Science & Business Media
ISBN: 1461393590
Category : Science
Languages : en
Pages : 480

Get Book Here

Book Description
As stated by its first editor, Dr. D. B. Roodyn, the primary goal of the series Subcellular Biochemistry is to achieve an integrated view of the cell by bringing together results from a wide range of different techniques and disciplines. This volume deals with the applications of fluorescence spectroscopy to membrane research. It seeks to present complementary biochemical and bio physical data on both the structure and the dynamics of biological membranes. Biophysics and biochemistry are improving more and more in their ability to study biomembranes, overlapping somewhat in this area and explaining the functioning of the whole cell in terms of the properties of its individual com ponents. Therefore, we have brought together an international group of experts in order to report on and review advances in fluorescence studies on biological membranes, thereby highlighting subcellular aspects. The first chapters present a critical evaluation of the current applications of dynamic and steady-state fluorescence techniques. Subsequent chapters dis cuss more specific applications in cells, biological membranes, and their con stituents (lipids, proteins).

Fluorescent Methods to Study Biological Membranes

Fluorescent Methods to Study Biological Membranes PDF Author: Yves Mely
Publisher: Springer Science & Business Media
ISBN: 3642331289
Category : Science
Languages : en
Pages : 484

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Book Description
Biological membranes play a central role in cell structure, shape and functions. However, investigating the membrane bilayer has proved to be difficult due to its highly dynamic and anisotropic structure, which generates steep gradients at the nanometer scale. Due to the decisive impact of recently developed fluorescence-based techniques, tremendous advances have been made in the last few years in our understanding of membrane characteristics and functions. In this context, the present book illustrates some of these major advances by collecting review articles written by highly respected experts. The book is organized in three parts, the first of which deals with membrane probes and model membranes. The second part describes the use of advanced quantitative and high-resolution techniques to explore the properties of biological membranes, illustrating the key progress made regarding membrane organization, dynamics and interactions. The third part is focused on the investigation of membrane proteins using the same techniques, and notably on the membrane receptors that play a central role in signaling pathways and therapeutic strategies. All chapters provide comprehensive information on membranes and their exploration for beginners in the field and advanced researchers alike.

Fluorescence Studies of Biological Membranes and Their Models

Fluorescence Studies of Biological Membranes and Their Models PDF Author: A. E. McGrath
Publisher:
ISBN:
Category :
Languages : en
Pages : 163

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Book Description


Fluorescence Spectroscopy in Biology

Fluorescence Spectroscopy in Biology PDF Author: Martin Hof
Publisher: Springer Science & Business Media
ISBN: 3540270043
Category : Science
Languages : en
Pages : 315

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Book Description
Volume 3 of this new series focuses on brandnew research and applications in biology, biophysics and other fields of life sciences. Many frontline researcher have contributed to this highly attractive and interdisciplinary volume which spans the entire field of present fluorescence spectroscopy including nanotechnology, membrane and DNA studies and fluorescence imaging in cancer research.

Fluorescence Spectroscopy and Microscopy in Biology

Fluorescence Spectroscopy and Microscopy in Biology PDF Author: Radek Šachl
Publisher: Springer Nature
ISBN: 3031303628
Category : Science
Languages : en
Pages : 532

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Book Description
This book provides the reader with an updated comprehensive view of the rapidly developing and fascinating field of fluorescence spectroscopy and microscopy. In recent years, fluorescence spectroscopy and microscopy have experienced rapid technological development, which has enabled the detection and monitoring of single molecules with high spatial and temporal resolution. Thanks to these developments, fluorescence has become an even more popular method in physical, biological and related fields. This book guides the reader through both basic and advanced fluorescence spectroscopy and microscopy approaches with a focus on their applications in membrane and protein biophysics. Each of the four parts: A - Fluorescence Spectroscopy, B - Fluorescence Microscopy, C - Applications of Fluorescence Spectroscopy and Microscopy to biological membranes and D - Applications of Fluorescence Spectroscopy to protein studies are written by experts within the field. The book is intended for both complete beginners who want to quickly orient themselves in the large number of existing fluorescent methods, as well as for advanced readers who are interested in particular methods and their proper use.

Fluorescence Studies of Biological Membrane Systems

Fluorescence Studies of Biological Membrane Systems PDF Author: Judith Ann Soares
Publisher:
ISBN:
Category :
Languages : en
Pages : 82

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Book Description


Spectroscopic Membrane Probes

Spectroscopic Membrane Probes PDF Author: Leslie M. Loew
Publisher: CRC Press
ISBN: 1351093703
Category : Science
Languages : en
Pages : 261

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Book Description
The general picture we have of membranes and of the properties of the proteins imbedded in them, has, arguably emerged directly from probe studies over the past 20 years. This work is designed to make these techniques accessible to a board audience of cell biologists. The techniques discussed revolve primarily around the fluorescence of membrane probes, but applications of light absorption and Raman scattering are included. In addition to reviews of the major applications, most chapters include information on the required apparatus, experimental design, data analysis, and potential pit falls.

Methods for Imaging Cell Membranes

Methods for Imaging Cell Membranes PDF Author: Luca Panconi
Publisher: CRC Press
ISBN: 1003823130
Category : Science
Languages : en
Pages : 137

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Book Description
· Measuring membrane protein distributions using single-molecule localisation microscopy (SMLM) · Measuring membrane protein dynamics and diffusion using fluorescence correlation spectroscopy (FCS) · Mapping membrane lipid backing using environmentally sensitive fluorescence probes · Mapping membrane thickness and rigidity using atomic force microscopy · Mapping membrane proteins and the cytoskeleton using electron microscopy

Molecular Biology of the Cell

Molecular Biology of the Cell PDF Author:
Publisher:
ISBN: 9780815332183
Category : Cells
Languages : en
Pages : 0

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Book Description


Time-Resolved Fluorescence Spectroscopy in Biochemistry and Biology

Time-Resolved Fluorescence Spectroscopy in Biochemistry and Biology PDF Author: R. Cundall
Publisher: Springer Science & Business Media
ISBN: 1475716346
Category : Science
Languages : en
Pages : 767

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Book Description
At the time that the editors conceived the idea of trying to organize the meeting on which the contents of this volume are based and which became, in March 1980, a NATO Advanced Study Institute, the techniques of time-resolved fluorescence spectroscopy, in both the nanosecond and sub-nanosecond time-domains, might reasonably have been said to be coming of age, both in their execution and in the analysis and interpretation of the results obtained. These techniques, then as now, comprised mainly a number of pulse methods using laser, flash-lamp or, most recently, synchrotron radiation. In addition, significant developments in the more classical phase approach had also rendered that method popular, utilizing either modulation of an otherwise continuous source or, again recently, the ultra-rapid pulse rate attainable with a synchrotron source. In general terms, time-resolved fluorescence studies are capable, under appropriate conditions, of supplying direct kinetic information on both photophysics and various aspects of molecular, macromolecular and supramolecular structure and dynamics. The nanosecond and sub-nanosecond time-scales directly probed render these techniques particularly appropriate in studying relaxation and fluctuation processes in macromolecules, particularly biopolymers (e. g. proteins, nucleic acids), in supramolecular assemblies such as cell membranes, and in a variety of relatively simpler model systems.