Evaluation of Agronomic, Seed Quality Traits, and Transcript Abundance in Conventional High Oleic Soybeans with Mutant FAD2-1A and FAD2-1B Genes PDF Download
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Author: Lauren Kathryn Richardson
Publisher:
ISBN:
Category : Linoleic acid
Languages : en
Pages : 85
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Book Description
Two soybean omega-6 fatty acid desaturase genes [FAD2-1A and FAD2- 1B] are responsible for converting oleic acid into linoleic acid (Okuley et al., 1994). Plant introductions [PI] 603452 and 283327 have naturally occurring mutations in FAD2-1A and FAD2-1B, respectively, which hinder the conversion of oleic acid to polyunsaturated fatty acids. The end result is more functional soybean oil with exceptionally high levels of oleic acid. The homozygous alleles of FAD2-1A and FAD2-1B were combined in a BC3F2:4 [backcross three, second filial generation selected, advanced to the fourth filial generation] population of forty-eight lines and were evaluated in Tennessee. Each line belonged to a double homozygous genotypic class: i) double wild-type [WT] (AABB), ii) mutant FAD2-1A with WT FAD2-1B (aaBB), iii) the converse (AAbb), and iv) double mutant (aabb). All genotypic groups yielded similarly to each other and to three high yielding checks (p
Author: Lauren Kathryn Richardson
Publisher:
ISBN:
Category : Linoleic acid
Languages : en
Pages : 85
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Book Description
Two soybean omega-6 fatty acid desaturase genes [FAD2-1A and FAD2- 1B] are responsible for converting oleic acid into linoleic acid (Okuley et al., 1994). Plant introductions [PI] 603452 and 283327 have naturally occurring mutations in FAD2-1A and FAD2-1B, respectively, which hinder the conversion of oleic acid to polyunsaturated fatty acids. The end result is more functional soybean oil with exceptionally high levels of oleic acid. The homozygous alleles of FAD2-1A and FAD2-1B were combined in a BC3F2:4 [backcross three, second filial generation selected, advanced to the fourth filial generation] population of forty-eight lines and were evaluated in Tennessee. Each line belonged to a double homozygous genotypic class: i) double wild-type [WT] (AABB), ii) mutant FAD2-1A with WT FAD2-1B (aaBB), iii) the converse (AAbb), and iv) double mutant (aabb). All genotypic groups yielded similarly to each other and to three high yielding checks (p
Author: Thang Cao La
Publisher:
ISBN:
Category : Electronic dissertations
Languages : en
Pages : 74
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Book Description
Soybeans with seed oil high in oleic acid content have been developed and are desired by oil processors because of improved oxidative stability for broader use in food, fuel and other products. Conventional soybean lines with 80% oleic acid content were generated by combining two mutant genes, mutant S117N FAD2-1A allele from 17D and mutant P137R FAD2-1B allele from PI283327. Thirty five F4:7 high oleic lines with these mutant FAD2-1A and FAD2-1B genes were compared to thirty five F4:7 normal or wild type oleic acid lines for seed contents of the five fatty acids common to soybean, total oil content, total protein content, and seed yield from five crosses grown in six environments. The high oleic genotypes averaged >75% oleic acid content and concentrations of palmitic and linolenic acids were significantly lower (30% lower) than their normal oleic acid counterparts in each environment. When averaged across all locations and populations, seed yield of the high oleic lines were within 2% of the normal oleic lines. Because of the negative correlation of protein with oil content and seed yield shown in many other studies, it was surprising that these high oleic lines averaged significantly higher in protein and oil with similar yield compared to normal oleic lines. These results provided evidence that high oleic soybeans can have comparable yields with the potential to generate more oil with greater functionality and higher protein meal for improved feed and food value compared to soybeans with normal oleic acid content from the same cross.
Author:
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Book Description
BACHLAVA, ELENI. Genetic Control of High Oleic Acid Seed Content in Soybean. (Under the direction of Andrea J. Cardinal and Ralph E. Dewey) The increase of oleic acid concentration in soybean [Glycine max (L.) Merr.] oil improves its nutritional value and oxidative stability; therefore, the incorporation of the high oleate trait in soybean germplasm is required for the commercial success of soybean oil. Little is known, however, about the response to selection for higher oleate content, which is dependent on its heritability in breeding populations, and the correlated responses of other fatty acids and agronomic traits to selection for oleate content, which depends on their genetic correlations with oleate. Furthermore, the lack of knowledge on the genetic factors underlying oleate variation in soybean oilseeds hampers the use of marker assisted selection for high oleate content in soybean breeding programs. The results of this study suggested that oleate heritability was sufficiently high that early generation selection can be effective when practiced on unreplicated lines grown at a single environment. Moreover, this study indicated that selection for higher oleate will result in lower linoleate, linolenate and palmitate content in soybean oil. Also, the significant negative correlation between oleate content and yield implied that the development of high oleate germplasm may be hindered by lower yields. The isoforms of FAD2 gene, which catalyze the desaturation of oleate to linoleate during fatty acid biosynthesis, were mapped in order to investigate their cosegregation with the oleate quantitative trait loci (QTLs) identified. FAD2-1A and FAD2-1B mapped on linkage groups O and I, respectively, while the closely linked FAD2-2A and FAD2-2B isoforms mapped on linkage group L of soybean genome. An oleate QTL with moderate effects was reported in the proximity of FAD2-1B on linkage group I. Also, an oleate QTL with moderate effects was detected on linkage group F, wh.
Author: Joel Hemingway
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Book Description
Soybean (Glycine max [L.] Merrill) oil is an economically important commodity worldwide with many uses. High levels of polyunsaturated fatty acids cause oxidative instability of the oil; however, the DP-3054231-1 transgene confers elevated oleic acid concentrations resulting in oil with increased oxidative stability. The first objective of this thesis was to study the effects of the DP-305423-1 transgene on agronomic and seed traits across multiple genetic backgrounds and environments. An equal number of high oleic (HO) and normal oleic (NO) BC1F4:F6 progeny from four unique populations were grown at four locations in Southern Ontario and two in Northern Iowa. Overall, the difference in mean yield between the HO and NO progeny varied across populations and locations and the HO progeny consistently had lower mean oil concentration and greater mean protein concentration. Differences in 100-seed weight were not consistent across populations or locations. Genomic selection (GS) has been shown to be a valuable tool for performing selection on complex quantitative traits, such as seed oil concentration in soybean. The second objective of the thesis was to evaluate multiple GS models for seed oil concentration using a low-density marker panel in bi-parental, high oleic soybean populations and compare prediction accuracies of six unique training populations (TPs). Prediction accuracy was calculated as the Pearson correlation coefficient between the predicted value of an individual and the 'true' phenotypic value, as determined through multi location field testing. Genomic best linear unbiased predictor (GBLUP) produced the greatest predictability across all populations and training sets, compared to BayesA and BayesB, which had similar predictive ability across populations. Generally, TPs consisting of more individuals had greater predictability; however, variations were observed across populations and models. TPs consisting of individuals from a single location had greater predictability of all genotypes than training populations of equal size comprised of individuals from both locations, indicating potential influence of marker x environment effects across training environments. These results show that genomic selection using a low marker density can be a valuable tool for increasing oil concentration in biparental high oleic, low linolenic soybeans populations.
Author: Alison Rose Willette
Publisher:
ISBN:
Category : Single nucleotide polymorphisms
Languages : en
Pages : 83
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Book Description
Author: Guohao He
Publisher:
ISBN:
Category : Electronic books
Languages : en
Pages : 0
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Book Description
Plants have numerous fatty acid desaturase (FAD) enzymes regulating the unsaturation of fatty acids, which are encoded by a FAD gene family. The FAD2 genes belong to such family and play a vital role in converting monounsaturated oleic acid to polyunsaturated linoleic acid. Oleic acid has the health benefits for humans, such as reduction in cholesterol level, antioxidation property, and industrial benefits like longer shelf life. The development of genotypes with high oleic acid content in seeds has become one of the primary goals in breeding oilseed plants. The identification and characterization of the FAD2 genes in plants have been an important step to better manipulate gene expression to improve the seed oil quality. The induction of mutations in FAD2 genes to reduce FAD2 enzyme activity has been an integral approach to generate genotypes with high oleic acid. This chapter will describe the FAD2 gene family in the model organism soybean and the correction of mutations in FAD2 genes with the increase of oleic acid content. Leveraging advanced research of FAD2 gene family in soybean promotes the study of FAD2 genes in other legume species, including peanut. The future perspectives and challenges associated with mutations in FAD2 genes will be discussed.
Author: National Research Council
Publisher: National Academies Press
ISBN: 0309170176
Category : Technology & Engineering
Languages : en
Pages : 342
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Book Description
Transgenic crops offer the promise of increased agricultural productivity and better quality foods. But they also raise the specter of harmful environmental effects. In this new book, a panel of experts examines: • Similarities and differences between crops developed by conventional and transgenic methods • Potential for commercialized transgenic crops to change both agricultural and nonagricultural landscapes • How well the U.S. government is regulating transgenic crops to avoid any negative effects. Environmental Effects of Transgenic Plants provides a wealth of information about transgenic processes, previous experience with the introduction of novel crops, principles of risk assessment and management, the science behind current regulatory schemes, issues in monitoring transgenic products already on the market, and more. The book discusses public involvementâ€"and public confidenceâ€"in biotechnology regulation. And it looks to the future, exploring the potential of genetic engineering and the prospects for environmental effects.
Author: Felipe Lopes da Silva
Publisher: Springer
ISBN: 3319574337
Category : Science
Languages : en
Pages : 439
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Book Description
This book was written by soybean experts to cluster in a single publication the most relevant and modern topics in soybean breeding. It is geared mainly to students and soybean breeders around the world. It is unique since it presents the challenges and opportunities faced by soybean breeders outside the temperate world.
Author: Thomas McKeon
Publisher: Elsevier
ISBN: 0128053852
Category : Business & Economics
Languages : en
Pages : 476
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Book Description
Industrial Oil Crops presents the latest information on important products derived from seed and other plant oils, their quality, the potential environmental benefit, and the latest trends in industrial uses. This book provides a comprehensive view of key oil crops that provide products used for fuel, surfactants, paints and coatings, lubricants, high-value polymers, safe plasticizers and numerous other products, all of which compete effectively with petroleum-derived products for quality and cost. Specific products derived from oil crops are a principle concern, and other fundamental aspects of developing oil crops for industrial uses are also covered. These include improvement through traditional breeding, and molecular, tissue culture and genetic engineering contributions to breeding, as well as practical aspects of what is needed to bring a new or altered crop to market. As such, this book provides a handbook for developing products from renewable resources that can replace those currently derived from petroleum. Led by an international team of expert editors, this book will be a valuable asset for those in product research and development as well as basic plant research related to oil crops. Up-to-date review of all the key oilseed crops used primarily for industrial purposes Highlights the potential for providing renewable resources to replace petroleum derived products Comprehensive chapters on biodiesel and polymer chemistry of seed oil Includes chapters on economics of new oilseed crops, emerging oilseed crops, genetic modification and plant tissue culture technology for oilseed improvement
Author: Jinguo Hu
Publisher: CRC Press
ISBN: 1439840490
Category : Science
Languages : en
Pages : 374
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Book Description
The sunflower has fascinated mankind for centuries. The oilseed sunflower contributes approximately ten percent of the world's plant-derived edible oil and the confection type sunflower holds a considerable share of the directly consumed snacks market. In addition, sunflower is also grown as an ornamental for cut flowers, as well as in home ga
