Capture and Analysis of Circulating Tumor Cells in Microfluidics PDF Download
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Author: Julien Autebert
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Book Description
Author: Julien Autebert
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Book Description
Author: Z. Hugh Fan
Publisher: John Wiley & Sons
ISBN: 1118915534
Category : Science
Languages : en
Pages : 479
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Book Description
Introduces the reader to Circulating Tumor Cells (CTCs), their isolation method and analysis, and commercially available platforms Presents the historical perspective and the overview of the field of circulating tumor cells (CTCs) Discusses the state-of-art methods for CTC isolation, ranging from the macro- to micro-scale, from positive concentration to negative depletion, and from biological-property-enabled to physical-property-based approaches Details commercially available CTC platforms Describes post-isolation analysis and clinical translation Provides a glossary of scientific terms related to CTCs
Author: Nika Nikbakht
Publisher:
ISBN: 9781321854633
Category :
Languages : en
Pages : 67
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Book Description
Circulating tumor cells (CTCs) are cells that shed into the vasculature from a primary tumor and circulate in the bloodstream. CTCs can be used to elucidate the molecular characterization of the tumor cells and to gauge the efficiency of therapeutic treatment in metastatic carcinoma patients. They can also be used to determine the primary site of the tumor in areas where the tumor is undetectable with traditional oncological imaging. The detection of CTCs has a substantial value for prognostic and therapeutic implications, but they are not easily detected because of their low cell count. Because microfluidic devices are useful for cell detection and diagnosis, can be easily obtained, and are less invasive than tissue biopsies, we have developed a microfluidic platform to capture CTCs using multiple capture targets to achieve a higher cell capture. We can selectively isolate the cancer cells using specific antibodies to the antigen capture target on the surface of malignant cells. The capture efficiency was evaluated by the flow rate, cell count, and antibody immobilization. Cancer cell lines that were known to have high expression for targeted ligands, specifically HER2, EGFR, EpCAM, and MUC-1, were tested with antibodies specific to these ligands. We obtained capture efficiency with these different capture targets on a single channel. This allowed us to develop a device with four parallel capture channels to run in series with the anticipation of achieving higher cell capture.
Author:
Publisher:
ISBN:
Category :
Languages : en
Pages : 171
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Book Description
Circulating tumor cells are cells that exist in the circulation and are accessible by a simple blood draw. The ability to collect and enumerate circulating tumor cells has revealed great prognostic and predictive value to an easily accessible cell population. Importantly, enumeration leaves behind much of the molecular information that is contained within this critical cell population, and the specific biological implication of these cells still remains unknown. To bring a more comprehensive analysis to circulating tumor cells, microfluidic methods have been used due to the natural precision inherent in the microscale that can be important when analyzing this exceedingly rare cell population. In this dissertation, methods to capture and analyze rare cell populations were developed to improve the study of circulating tumor cells and other rare cell samples. These techniques were developed with a focus on simple fundamental physical phenomena of the microscale to create assays that are easy to operate and integrate. The resulting CTC protocol has demonstrated the capability of isolating multiple types of rare cells (CTCs, lung lavage samples) in multiple diseases (prostate cancer, lung cancer, kidney cancer, breast cancer, melanoma). Further, the assessment of these cells was taken beyond enumeration to demonstrate the benefit of molecular assessment of this cell population. Applying the device to clinical studies of prostate cancer and assessing activity of the androgen receptor in CTCs has demonstrated the ability to assess and potentially predict therapeutic benefit of patients undergoing AR-targeting therapy. Throughout the development of these assays, patient samples were used to keep the methods grounded in clinical utility. The technologies developed in this dissertation are focused on expanding the biological and clinical implications of circulating tumor cells toward improving patient care.
Author: Jin-Ming Lin
Publisher: Springer
ISBN: 9811053944
Category : Science
Languages : en
Pages : 435
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Book Description
This book presents a detailed overview of the design, formatting, application, and development of microfluidic chips in the context of cell biology research, enumerating each element involved in microfluidics-based cell analysis, discussing its history, status quo, and future prospects, It also offers an extensive review of the research completed in the past decade, including numerous color figures. The individual chapters are based on the respective authors' studies and experiences, providing tips from the frontline to help researchers overcome bottlenecks in their own work. It highlights a number of cutting-edge techniques, such as 3D cell culture, microfluidic droplet technique, and microfluidic chip-mass spectrometry interfaces, offering a first-hand impression of the latest trends in the field and suggesting new research directions. Serving as both an elementary introduction and advanced guidebook, the book interests and inspires scholars and students who are currently studying microfluidics-based cell analysis methods as well as those who wish to do so.
Author: Yu-Yen Huang
Publisher:
ISBN:
Category :
Languages : en
Pages : 216
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Book Description
Circulating tumor cells (CTCs) are known to escape from the primary tumor site and may settle down at the distant organ to grow a second tumor. CTCs are one of causes initiating carcinoma metastasis. Detection of CTCs has been considered to be valuable for cancer management, including diagnosis, prognosis, and clinical treatment management. However, efficient isolation, enumeration, characterization, and genetic analysis of CTCs in whole-blood samples from cancer patients are very challenging due to their extremely low concentration and rare nature (per CTC in blood cells is 1:106-109). With the increasing worldwide death rate associated with cancer, there is a desperate demand for a high-sensitivity, high-throughput, and low-cost detection and separation system. My doctoral research focused on the design and fabrications of the screening system for the detection of CTCs with further analysis of captured CTCs, such as immunofluoresce staining and fluorescence in-situ hybridization (FISH). The distinct significance of this research is that the development of the computer-controlled rotational holder with a series of six inverted microfluidic chips reduced the cost by significantly reducing the consumption of magnetic carriers (25% of the consumed amount used in the commercial CellSearch® system), increasing the capture efficiency by manipulating the blood sedimentation in the microchannel, enhancing the system stability by integrating the micromagnets on the plain glass slide substrate, and achieving high throughput because of the high flow rate (2.5 mL/hr) and large screening volume (screening up to six chips in parallel with each containing 2.5 mL of blood). Immunofluorescence staining and the FISH method have been performed to prove the capability of the system. In addition, the system has been successfully applied for patient samples screening. The incorporation of micromagnets has demonstrated that micromagnets provide localized magnetic forces to scatter the target cancer cells and free nanoparticles throughout the whole channel substrate to increase the channel space usage by 13%. Four cancer cell lines, including COLO 205 (colorectal cancer), SK-BR-3 (breast cancer), MCF-7 (breast cancer), and PC3 (prostate cancer), were spiked in blood samples from healthy donors to verify high capture efficiency of the developed system. On average, over a 97% capture rate was demonstrated for all cell lines. Moreover, the developed screening system has been successfully screened over 40 patient samples, including metastatic lung cancer, breast cancer, prostate cancer, and colorectal cancer. After capture of CTCs, immunofluorescence staining was used to identified the captured cancer cells and the FISH method was performed to characterize the isolated cancer cells by studying the gene expression of CTCs from breast cancer. The proposed automated immunomagnetic microchip-based screening system shows high capture efficiency (average 97% for three spiked cell lines), high throughput (15 mL of blood sample per screening), high sensitivity, high specificity, and low nanoparticle consumption (75% less than CellSearch® system). The screening system provides great promise as a clinical tool for early cancer diagnosis, diagnosis, personalized therapy, and treatment monitoring.
Author: Sweta Gupta
Publisher:
ISBN: 9781124880983
Category :
Languages : en
Pages : 48
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Book Description
Viable tumor cells that are disseminated in the blood stream, also known as circulating tumor cells (CTCs), are often the cause of metastasis in cancer patients. Although these cells are rare in blood, they can be isolated and used to study various aspects of the tumor such as molecular characterization of the tumor cells, effectiveness of treatment therapies in metastatic carcinoma patients, and also to determine the primary site of the tumor in cases where the tumor itself is undetectable. Previous researches have demonstrated microfluidic platforms capable of selectively capturing rare cells from raw liquid samples, using adhesion-mediated binding of the target cells with complementary ligand proteins that are immobilized on arrays of micropillars. In these systems, the circular or square shaped micropillars which provide increased surface area for cell-protein interactions, were fabricated on a silicon chip by an expensive and skillfully demanding technique called deep reactive ion etching (DRIE) [1,2]. Based on the concept of protein-coated micropillars, we used soft lithographic techniques to develop microfluidic devices using poly(dimethylsiloxane) (PDMS) polymer. PDMS molds consisting of thirty five different device designs with varied micropillar features like shape, size, spacing, and array arrangement were fabricated. The devices were tested with five different cancer cell lines, at different flow rates and cell concentrations, and a comparative study was performed to determine the most efficient design in terms of cell capture efficiency. Some designs achieved mean capture yields of>45%, thereby making this low-cost, quick and easy technique an attractive cancer screening tool.
Author: Karla Perez Toralla
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Book Description
Author: Yu Song
Publisher: John Wiley & Sons
ISBN: 3527341064
Category : Science
Languages : en
Pages : 576
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Book Description
The first book offering a global overview of fundamental microfluidics and the wide range of possible applications, for example, in chemistry, biology, and biomedical science. As such, it summarizes recent progress in microfluidics, including its origin and development, the theoretical fundamentals, and fabrication techniques for microfluidic devices. The book also comprehensively covers the fluid mechanics, physics and chemistry as well as applications in such different fields as detection and synthesis of inorganic and organic materials. A useful reference for non-specialists and a basic guideline for research scientists and technicians already active in this field or intending to work in microfluidics.
Author: Michail Ignatiadis
Publisher: Springer Science & Business Media
ISBN: 3642281605
Category : Medical
Languages : en
Pages : 245
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Book Description
This important book provides up-to-date information on a series of topical issues relating to the approach to minimal residual disease in breast cancer patients. It first explains how the study of minimal residual disease and circulating and disseminated tumor cells (CTCs/DTCs) can assist in the understanding of breast cancer metastasis. A series of chapters then discuss the various technologies available for the detection and characterization of CTCs and DTCs, pinpointing their merits and limitations. Detailed consideration is given to the relevance of CTCs and DTCs, and their detection, to clinical research and practice. The role of other blood-based biomarkers is also addressed, and the closing chapters debate the challenges facing drug and biomarker co-development and the use of CTCs for companion diagnostic development. This book will be of interest and assistance to all who are engaged in the modern management of breast cancer.
