Biochemical and Structural Analysis of the Protein Machinery Required for Outer Membrane Protein Biogenesis in Escherichia Coli PDF Download
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Author: Seokhee Kim
Publisher:
ISBN: 9780549876847
Category : Escherichia coli
Languages : en
Pages : 212
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Book Description
Beta-barrel transmembrane proteins are found only in the outer membranes of Gram-negative bacteria and the eukaryotic organelles of bacterial origin, mitochondria and chloroplasts. These proteins are assembled in the membrane by a machine whose central component is a highly conserved protein; in Escherichia coli, this protein is known as YaeT. The multi-protein machine is believed to mediate the targeting, folding and insertion of other beta-barrel proteins, but the molecular mechanisms by which it performs these functions are unknown. In order to improve our understanding of these processes, structural information about the YaeT complex was obtained using biochemical and crystallographic tools.
Author: Seokhee Kim
Publisher:
ISBN: 9780549876847
Category : Escherichia coli
Languages : en
Pages : 212
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Book Description
Beta-barrel transmembrane proteins are found only in the outer membranes of Gram-negative bacteria and the eukaryotic organelles of bacterial origin, mitochondria and chloroplasts. These proteins are assembled in the membrane by a machine whose central component is a highly conserved protein; in Escherichia coli, this protein is known as YaeT. The multi-protein machine is believed to mediate the targeting, folding and insertion of other beta-barrel proteins, but the molecular mechanisms by which it performs these functions are unknown. In order to improve our understanding of these processes, structural information about the YaeT complex was obtained using biochemical and crystallographic tools.
Author: R.E. Dalbey
Publisher: Elsevier
ISBN: 0080526098
Category : Science
Languages : en
Pages : 297
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Book Description
The incentive for putting together Volume 4 of this series was to review the wealth of new information that has become available in prokaryotic organisms in protein export and membrane biogenesis. Just in the last several years, protein translocation has now been efficiently reconstituted using defined components and the mechanism by which proteins are moved across membrane bilayers is now being examined at a higher resolution. In addition, because of a new technical breakthrough using osmolytes, it is now possible to reconstitute a number of channel proteins, ATPase, receptors, and transporters. In many cases, it is possible to successfully predict the membrane topology of these types of proteins using both "hydrophobicity analysis" and the "positive inside" rule. In this volume, two chapters focus on protein translocation across membranes (Biochemical Analyses of Components Comprising the Protein Translocation Machinery of E. Coli; Protein Translocation Genetics), while several others on how proteins assemble into the ineer membrane of E. Coli (Membrane Protein Assembly; Membrane Insertion of Small Proteins: Evolutionary and Functional Aspects; Pigment-Protein Complex Assembly in Rhodobacter sphaeroides and Rhodobacter Capsulatus). Other sections review recent progress on transporters (Identification and Reconstitution of Anion Exchange Mechanisms in Bacteria; Helic Packing in the C-Terminal Half of Lactose Permease) and signal transduction (Mechanism of Transmembrane Signaling in Osmoregulation) as well as the assembly of prints into the outer membrane (Export and Assembly of Outer Membrane Proteins in E. coli). Although the emphasis of the book is on proteins, the role of phospholipids in controlling various cell surface processes is reviewed (Role of Phospholipids in coli Cell Function). I should point out the reason for the rapid progress in bacteria research is because of the possibility to apply biochemistry and genetics in this organism.
Author: Susan Buchanan
Publisher: Humana
ISBN: 9781493928705
Category : Science
Languages : en
Pages : 0
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Book Description
This volume is comprised of a collection of experimental protocols for common techniques and strategies used to study the biogenesis of b-barrel outer membrane proteins in Gram-negative bacteria. The BAM Complex: Methods and Protocols guides readers through methods on the function of the BAM complex, the roles played by each of the individual components, the expression and purification of the components, crystallization and structure determination of the components, and how the individual Bam components may assemble into a functional complex. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, The BAM Complex: Methods and Protocols will serve as an invaluable reference for those interested in studying the BAM complex.
Author: Jos A.F. Op den Kamp
Publisher: Springer Science & Business Media
ISBN: 3642788467
Category : Science
Languages : en
Pages : 358
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Book Description
The Advanced Study Institute on "Structure, Biogenesis and Dynamics of Biological Membranes, held in Cargese from June 14-26, 1993, has been dealing with four major topics in membrane biochemistry today: lipid dynamics and lipid-protein interactions, protein translocation and insertion, intracellular traffic aud protein structure and folding. The lecturers discussed these topics starting from several disciplines, including biochemistry, cell biology, genetics, and biophysics. This wayan interdisciplinary and very inte~sting view on biological membrane systems was obtained. At first an extensive overview of -mainly biophysical -techniques which can be used to study dynamic processes in membranes was presented. Sophisticated approaches such as ESR and NMR have been applied succesfully to unravel details of specific lipid-protein interactions. x ray analysis provides detailed structural information of several proteins and the possible implications for protein functions. Information obtained this way is complemented by studies on mechanisms and kinetics of protein folding. The latter information is indispensable when discussing protein translocation and insertion: proces:;es in which folding and unfolding play essential roles. Extensive insight was offered in the complicated machinery of phospholipid biosynthesis. In particular, the application of sophisticated genetic techniques has allowed a better understanding of the mechanisms regulating the synthetic machinery and detailed studies on a variety of mutants, lacking one or more of the essential enzymes, have resulted in the beginning of a bL!:
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Author: Kelly (Hyojin) Kim
Publisher:
ISBN:
Category : Escherichia coli
Languages : en
Pages : 0
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Book Description
The outer membrane (OM) is a unique structural feature of Gram-negative bacteria. Residing within the outer membrane are [beta]-barrel outer membrane proteins (OMPs) that serve many important cellular functions. As proper folding and assembly of these proteins are crucial for cell viability, Gram-negative bacteria possess a specialized proteinaceous machine, known as the BAM ([beta]-barrel assembly machinery) complex, to catalyze the folding and membrane insertion of OMPs. In Escherichia coli, the BAM complex consists of five proteins: one [beta]-barrel membrane protein -- BamA, and four lipoproteins -- BamB, BamC, BamD, and BamE. The roles of the individual components and how they are arranged into the BAM complex to function together is not yet clearly understood. During the course of this thesis project, I determined the structures of E. coli BamB, BamC, BamE and the BamCD subcomplex. Analysis of the conserved residues and the molecular surface properties of these solved structures helped to identify potential protein-protein interaction sites on each lipoprotein. For example, BamC has two 'helix-grip' domains that are ideally shaped to accommodate [alpha]-helices. BamB, on the other hand, has a [beta]-propeller fold that could potentially interact with BamA or substrates via [beta]-augmentation, a mode of interaction in which a pre-existing [beta]-sheet is augmented by an addition of a [beta]-strand of another protein. Comparing the solved structures with their structural homologs with known functions has also provided important clues about the functional roles of each lipoprotein. BamD structure, for example, closely resembles the binding pocket of a peroxisomal targeting signal receptor PEX5, suggesting a similar substrate recognition function for BamD. Interestingly, our BamCD complex structure shows that the putative substrate binding pocket of BamD is bound and blocked by the conserved unstructured N-terminal region of BamC. This suggests a possibility that BamC may have a regulatory function. The structural and interaction data acquired from this thesis project contributes to a better understanding of the BAM complex structure and provides a platform for future research driven by structure-based hypotheses.
Author: Gunnar von Heijne
Publisher: R. G. Landes
ISBN:
Category : Science
Languages : en
Pages : 300
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Book Description
Author: Raffaele Ieva
Publisher: Springer Nature
ISBN: 1071637347
Category :
Languages : en
Pages : 388
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Book Description
Author: B. Oudega
Publisher: Springer Science & Business Media
ISBN: 9401000956
Category : Science
Languages : en
Pages : 404
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Book Description
For bacteria ..."the times are achanging"... The genomes of over 60 different bacteria have now been sequenced, and we know a lot about the important research organism Escherichia coli, the important industrial organism Bacillus subtilis, and about important plant and human pathogens. It will not take long before we know all the gene products and their functions of a few of these bacteria. Some of us already begin to think about a digital model E. coli or Bacillus cell. For that end we need to know all the physiological activities and metabolic routes of the cell. But in addition we like to know how things work at the molecular level and how protein and membranes as well as other (macromolecular) structures work together to carry out specific cell functions. Protein Secretion Pathways in Bacteria describes all the known folding and targeting routes of inner and outer membrane proteins as well as of proteins that are secreted by several specific export routes. The book gives detailed molecular information about the structures that are important for the different mechanisms involved. This is a valuable contribution to the understanding of how rather simple and yet complex bacterial cells work.
Author: Anders Liljas
Publisher: World Scientific
ISBN: 9814531804
Category : Science
Languages : en
Pages : 494
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Book Description
This highly illustrated book provides an up-to-date description of the structure and function of the translation system including ribosomes, tRNAs, translation factors, antibiotics and aminoacyl-tRNA synthetases.Research on translation is undergoing rapid changes and is receiving significant attention as evidenced by the Nobel Prize in Chemistry 2009. The structural research by crystallography and cryo-EM forms part of an interactive framework that involves biochemistry and molecular computation. The book provides a comprehensive overview of translation in light of the structural results. It is a valuable resource for scientists in this and related fields, as well as for students taking courses with a focus on translation.There is no other book in this field currently except the previous edition of this book. The authors have for a long time worked in the field of structure and function of the translation system.
Author: David S. Eisenberg
Publisher: Academic Press
ISBN: 0080888712
Category : Science
Languages : en
Pages : 183
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Book Description
Published continuously since 1944, the Advances in Protein Chemistry and Structural Biology serial has been a continuous, essential resource for protein chemists. Covering reviews of methodology and research in all aspects of protein chemistry, including purification/expression, proteomics, modeling and structural determination and design, each volume brings forth new information about protocols and analysis of proteins while presenting the most recent findings from leading experts in a broad range of protein-related topics. This eclectic volume features articles on a variety of topical subjects. - Includes new information about protocols and analysis of proteins - Eclectic volume presents chapters by a wide range of leading experts - Presents new, cutting-edge information that will serve as an essential addition to any bookshelf or laboratory
