Accelerated Generation of Elite Inbreds in Maize Using Doubled Haploid Technology PDF Download
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Author: Firoz Hossain
Publisher:
ISBN:
Category : Electronic books
Languages : en
Pages : 0
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Book Description
The creation of homozygous parental lines for hybrid development is one of the key components of commercial maize breeding programs. It usually takes up to 6 to 7 generations of selfing to obtain homozygous inbreds from the initial cross using the conventional pedigree method. Using doubled haploid (DH) method, concurrent fixation of all the genes covering entire chromosomes is possible within a single generation. For generation of DH lines, haploids are generated first by several means such as in-vitro method using tissue culture technique and in-vivo method using the haploid inducer (HI) lines. Of which, tissue culture-based methods have shown little promise for large-scale DH production as it needs good infrastructures and technical requirements. In contrast, inducer-based method provides more optimistic solutions for large-scale DH lines production. Due to its rapidity, DH technology is now being adopted in many countries including India for reducing the breeding cycle.
Author: Firoz Hossain
Publisher:
ISBN:
Category : Electronic books
Languages : en
Pages : 0
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Book Description
The creation of homozygous parental lines for hybrid development is one of the key components of commercial maize breeding programs. It usually takes up to 6 to 7 generations of selfing to obtain homozygous inbreds from the initial cross using the conventional pedigree method. Using doubled haploid (DH) method, concurrent fixation of all the genes covering entire chromosomes is possible within a single generation. For generation of DH lines, haploids are generated first by several means such as in-vitro method using tissue culture technique and in-vivo method using the haploid inducer (HI) lines. Of which, tissue culture-based methods have shown little promise for large-scale DH production as it needs good infrastructures and technical requirements. In contrast, inducer-based method provides more optimistic solutions for large-scale DH lines production. Due to its rapidity, DH technology is now being adopted in many countries including India for reducing the breeding cycle.
Author:
Publisher: CIMMYT
ISBN: 6078263005
Category :
Languages : en
Pages : 57
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Book Description
Author: Haiping Wang
Publisher: BoD – Books on Demand
ISBN: 1803561041
Category : Technology & Engineering
Languages : en
Pages : 424
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Book Description
Conventional plant breeding has significantly improved crop yield, disease resistance, and adaptability to the environment; however, it is difficult to cultivate breakthrough new varieties using conventional breeding techniques. As such, new and novel breeding techniques are being developed. This book presents a comprehensive overview of plant breeding. It is organized into four sections on 'Genetic Resources for Plant Breeding”, 'Breeding Theory and Strategy”, 'Breeding Practice and Cases”, and 'The Perspective for Plant Breeding”.
Author: Jauhar Ali
Publisher: Springer Nature
ISBN: 3030665305
Category : Technology & Engineering
Languages : en
Pages : 507
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Book Description
This book is open access under a CC BY 4.0 license. By 2050, human population is expected to reach 9.7 billion. The demand for increased food production needs to be met from ever reducing resources of land, water and other environmental constraints. Rice remains the staple food source for a majority of the global populations, but especially in Asia where ninety percent of rice is grown and consumed. Climate change continues to impose abiotic and biotic stresses that curtail rice quality and yields. Researchers have been challenged to provide innovative solutions to maintain, or even increase, rice production. Amongst them, the ‘green super rice’ breeding strategy has been successful for leading the development and release of multiple abiotic and biotic stress tolerant rice varieties. Recent advances in plant molecular biology and biotechnologies have led to the identification of stress responsive genes and signaling pathways, which open up new paradigms to augment rice productivity. Accordingly, transcription factors, protein kinases and enzymes for generating protective metabolites and proteins all contribute to an intricate network of events that guard and maintain cellular integrity. In addition, various quantitative trait loci associated with elevated stress tolerance have been cloned, resulting in the detection of novel genes for biotic and abiotic stress resistance. Mechanistic understanding of the genetic basis of traits, such as N and P use, is allowing rice researchers to engineer nutrient-efficient rice varieties, which would result in higher yields with lower inputs. Likewise, the research in micronutrients biosynthesis opens doors to genetic engineering of metabolic pathways to enhance micronutrients production. With third generation sequencing techniques on the horizon, exciting progress can be expected to vastly improve molecular markers for gene-trait associations forecast with increasing accuracy. This book emphasizes on the areas of rice science that attempt to overcome the foremost limitations in rice production. Our intention is to highlight research advances in the fields of physiology, molecular breeding and genetics, with a special focus on increasing productivity, improving biotic and abiotic stress tolerance and nutritional quality of rice.
Author: Jeff L. Bennetzen
Publisher: Springer Science & Business Media
ISBN: 0387778632
Category : Technology & Engineering
Languages : en
Pages : 785
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Book Description
Maize is one of the world’s highest value crops, with a multibillion dollar annual contribution to agriculture. The great adaptability and high yields available for maize as a food, feed and forage crop have led to its current production on over 140 million hectares worldwide, with acreage continuing to grow at the expense of other crops. In terms of tons of cereal grain produced worldwide, maize has been number one for many years. Moreover, maize is expanding its contribution to non-food uses, including as a major source of ethanol as a fuel additive or fuel alternative in the US. In addition, maize has been at the center of the transgenic plant controversy, serving as the first food crop with released transgenic varieties. By 2008, maize will have its genome sequence released, providing the sequence of the first average-size plant genome (the four plant genomes that are now sequenced come from unusually tiny genomes) and of the most complex genome sequenced from any organism. Among plant science researchers, maize has the second largest and most productive research community, trailing only the Arabidopsis community in scale and significance. At the applied research and commercial improvement levels, maize has no peers in agriculture, and consists of thousands of contributors worthwhile. A comprehensive book on the biology of maize has not been published. The "Handbook of Maize: the Genetics and Genomics" center on the past, present and future of maize as a model for plant science research and crop improvement. The books include brief, focused chapters from the foremost maize experts and feature a succinct collection of informative images representing the maize germplasm collection.
Author: Satbir Singh Gosal
Publisher: Springer Nature
ISBN: 3030418669
Category : Science
Languages : en
Pages : 455
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Book Description
Plant improvement has shifted its focus from yield, quality and disease resistance to factors that will enhance commerical export, such as early maturity, shelf life and better processing quality. Conventional plant breeding methods aiming at the improvement of a self-pollinating crop, such as wheat, usually take 10-12 years to develop and release of the new variety. During the past 10 years, significant advances have been made and accelerated methods have been developed for precision breeding and early release of crop varieties. This work summarizes concepts dealing with germplasm enhancement and development of improved varieties based on innovative methodologies that include doubled haploidy, marker assisted selection, marker assisted background selection, genetic mapping, genomic selection, high-throughput genotyping, high-throughput phenotyping, mutation breeding, reverse breeding, transgenic breeding, shuttle breeding, speed breeding, low cost high-throughput field phenotyping, etc. It is an important reference with special focus on accelerated development of improved crop varieties.
Author: Arnel R. Hallauer
Publisher: Springer Science & Business Media
ISBN: 1441907661
Category : Science
Languages : en
Pages : 669
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Book Description
Maize is used in an endless list of products that are directly or indirectly related to human nutrition and food security. Maize is grown in producer farms, farmers depend on genetically improved cultivars, and maize breeders develop improved maize cultivars for farmers. Nikolai I. Vavilov defined plant breeding as plant evolution directed by man. Among crops, maize is one of the most successful examples for breeder-directed evolution. Maize is a cross-pollinated species with unique and separate male and female organs allowing techniques from both self and cross-pollinated crops to be utilized. As a consequence, a diverse set of breeding methods can be utilized for the development of various maize cultivar types for all economic conditions (e.g., improved populations, inbred lines, and their hybrids for different types of markets). Maize breeding is the science of maize cultivar development. Public investment in maize breeding from 1865 to 1996 was $3 billion (Crosbie et al., 2004) and the return on investment was $260 billion as a consequence of applied maize breeding, even without full understanding of the genetic basis of heterosis. The principles of quantitative genetics have been successfully applied by maize breeders worldwide to adapt and improve germplasm sources of cultivars for very simple traits (e.g. maize flowering) and very complex ones (e.g., grain yield). For instance, genomic efforts have isolated early-maturing genes and QTL for potential MAS but very simple and low cost phenotypic efforts have caused significant and fast genetic progress across genotypes moving elite tropical and late temperate maize northward with minimal investment. Quantitative genetics has allowed the integration of pre-breeding with cultivar development by characterizing populations genetically, adapting them to places never thought of (e.g., tropical to short-seasons), improving them by all sorts of intra- and inter-population recurrent selection methods, extracting lines with more probability of success, and exploiting inbreeding and heterosis. Quantitative genetics in maize breeding has improved the odds of developing outstanding maize cultivars from genetically broad based improved populations such as B73. The inbred-hybrid concept in maize was a public sector invention 100 years ago and it is still considered one of the greatest achievements in plant breeding. Maize hybrids grown by farmers today are still produced following this methodology and there is still no limit to genetic improvement when most genes are targeted in the breeding process. Heterotic effects are unique for each hybrid and exotic genetic materials (e.g., tropical, early maturing) carry useful alleles for complex traits not present in the B73 genome just sequenced while increasing the genetic diversity of U.S. hybrids. Breeding programs based on classical quantitative genetics and selection methods will be the basis for proving theoretical approaches on breeding plans based on molecular markers. Mating designs still offer large sample sizes when compared to QTL approaches and there is still a need to successful integration of these methods. There is a need to increase the genetic diversity of maize hybrids available in the market (e.g., there is a need to increase the number of early maturing testers in the northern U.S.). Public programs can still develop new and genetically diverse products not available in industry. However, public U.S. maize breeding programs have either been discontinued or are eroding because of decreasing state and federal funding toward basic science. Future significant genetic gains in maize are dependent on the incorporation of useful and unique genetic diversity not available in industry (e.g., NDSU EarlyGEM lines). The integration of pre-breeding methods with cultivar development should enhance future breeding efforts to maintain active public breeding programs not only adapting and improving genetically broad-based germplasm but also developing unique products and training the next generation of maize breeders producing research dissertations directly linked to breeding programs. This is especially important in areas where commercial hybrids are not locally bred. More than ever public and private institutions are encouraged to cooperate in order to share breeding rights, research goals, winter nurseries, managed stress environments, and latest technology for the benefit of producing the best possible hybrids for farmers with the least cost. We have the opportunity to link both classical and modern technology for the benefit of breeding in close cooperation with industry without the need for investing in academic labs and time (e.g., industry labs take a week vs months/years in academic labs for the same work). This volume, as part of the Handbook of Plant Breeding series, aims to increase awareness of the relative value and impact of maize breeding for food, feed, and fuel security. Without breeding programs continuously developing improved germplasm, no technology can develop improved cultivars. Quantitative Genetics in Maize Breeding presents principles and data that can be applied to maximize genetic improvement of germplasm and develop superior genotypes in different crops. The topics included should be of interest of graduate students and breeders conducting research not only on breeding and selection methods but also developing pure lines and hybrid cultivars in crop species. This volume is a unique and permanent contribution to breeders, geneticists, students, policy makers, and land-grant institutions still promoting quality research in applied plant breeding as opposed to promoting grant monies and indirect costs at any short-term cost. The book is dedicated to those who envision the development of the next generation of cultivars with less need of water and inputs, with better nutrition; and with higher percentages of exotic germplasm as well as those that pursue independent research goals before searching for funding. Scientists are encouraged to use all possible breeding methodologies available (e.g., transgenics, classical breeding, MAS, and all possible combinations could be used with specific sound long and short-term goals on mind) once germplasm is chosen making wise decisions with proven and scientifically sound technologies for assisting current breeding efforts depending on the particular trait under selection. Arnel R. Hallauer is C. F. Curtiss Distinguished Professor in Agriculture (Emeritus) at Iowa State University (ISU). Dr. Hallauer has led maize-breeding research for mid-season maturity at ISU since 1958. His work has had a worldwide impact on plant-breeding programs, industry, and students and was named a member of the National Academy of Sciences. Hallauer is a native of Kansas, USA. José B. Miranda Filho is full-professor in the Department of Genetics, Escola Superior de Agricultura Luiz de Queiroz - University of São Paulo located at Piracicaba, Brazil. His research interests have emphasized development of quantitative genetic theory and its application to maize breeding. Miranda Filho is native of Pirassununga, São Paulo, Brazil. M.J. Carena is professor of plant sciences at North Dakota State University (NDSU). Dr. Carena has led maize-breeding research for short-season maturity at NDSU since 1999. This program is currently one the of the few public U.S. programs left integrating pre-breeding with cultivar development and training in applied maize breeding. He teaches Quantitative Genetics and Crop Breeding Techniques at NDSU. Carena is a native of Buenos Aires, Argentina. http://www.ag.ndsu.nodak.edu/plantsci/faculty/Carena.htm
Author: Rajeev K. Varshney
Publisher: Springer Science & Business Media
ISBN: 1402062974
Category : Technology & Engineering
Languages : en
Pages : 517
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Book Description
This superb volume provides a critical assessment of genomics tools and approaches for crop breeding. Volume 1 presents the status and availability of genomic resources and platforms, and also devises strategies and approaches for effectively exploiting genomics research. Volume 2 goes into detail on a number of case studies of several important crop and plant species that summarize both the achievements and limitations of genomics research for crop improvement.
Author: Marcelo J. Carena
Publisher: Springer Science & Business Media
ISBN: 0387722971
Category : Technology & Engineering
Languages : en
Pages : 432
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Book Description
Agriculture depends on improved cultivars, and cultivars are developed through proper plant breeding. Unfortunately, applied plant breeding programs that are focused on cereal commodity crops are under serious erosion because of lack of funding. This loss of public support affects breeding continuity, objectivity, and, perhaps equally important, the training of future plant breeders and the utilization and improvement of plant genetic resources currently available. Breeding programs should focus not only on short-term research goals but also on long-term genetic improvement of germplasm. The research products of breeding programs are important not only for food security but also for commodity-oriented public and private programs, especially in the fringes of crop production. Breeding strategies used for long-term selection are often neglected but the reality is that long-term research is needed for the success of short-term products. An excellent example is that genetically broad-based public germplasm has significantly been utilized and recycled by industry, producing billions of dollars for industry and farmers before intellectual property rights were available. Successful examples of breeding continuity have served the sustainable cereal crop production that we currently have. The fact that farmers rely on public and private breeding institutions for solving long-term challenges should influence policy makers to reverse this trend of reduced funding. Joint cooperation between industry and public institutions would be a good example to follow. The objective of this volume is to increase the utilization of useful genetic resources and increase awareness of the relative value and impact of plant breeding and biotechnology. That should lead to a more sustainable crop production and ultimately food security. Applied plant breeding will continue to be the foundation to which molecular markers are applied. Focusing useful molecular techniques on the right traits will build a strong linkage between genomics and plant breeding and lead to new and better cultivars. Therefore, more than ever there is a need for better communication and cooperation among scientists in the plant breeding and biotechnology areas. We have an opportunity to greatly enhance agricultural production by applying the results of this research to meet the growing demands for food security and environmental conservation. Ensuring strong applied plant breeding programs with successful application of molecular markers will be essential in ensuring such sustainable use of plant genetic resources.
Author: Alisher Touraev
Publisher: Springer Science & Business Media
ISBN: 140208854X
Category : Science
Languages : en
Pages : 348
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Book Description
The importance of haploids is well known to geneticists and plant breeders. The discovery of anther-derived haploid Datura plants in 1964 initiated great excitement in the plant breeding and genetics communities as it offered shortcuts in producing highly desirable homozygous plants. Unfortunately, the expected revolution was slow to materialise due to problems in extending methods to other species, including genotypic dependence, recalcitrance, slow development of tissue culture technologies and a lack of knowledge of the underlying processes. Recent years have witnessed great strides in the research and application of haploids in higher plants. After a lull in activities, drivers for the resurgence have been: (1) development of effective tissue culture protocols, (2) identification of genes c- trolling embryogenesis, and (3) large scale and wide spread commercial up-take in plant breeding and plant biotechnology arenas. The first major international symposium on “Haploids in Higher Plants” took place in Guelph, Canada in 1974. At that time there was much excitement about the potential benefits, but in his opening address Sir Ralph Riley offered the following words of caution: “I believe that it is quite likely that haploid research will contr- ute cultivars to agriculture in several crops in the future. However, the more extreme claims of the enthusiasts for haploid breeding must be treated with proper caution. Plant breeding is subject from time to time to sweeping claims from ent- siastic proponents of new procedures.
