Author: Mariam Iftikhar
Publisher:
ISBN: 9780355066067
Category :
Languages : en
Pages : 128
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Book Description
Molecular motions of proteins and their flexibility induce conformational states required for enzyme catalysis, signal transduction, and protein-protein interactions. However, the mechanisms for protein transitions between conformational states are often poorly understood, especially in the millisecond to microsecond range where conventional optical techniques and computational modeling are most limited. To investigate the microsecond dynamics of enzymes, single-walled carbon nanotube -- field-effect transistors (SWCNT-FETs) were used as single-molecule biosensors. The SWCNT-FETs have sufficient sensitivity and bandwidth to monitor the conformational motions and processivity of an individual cAMP-dependent protein kinase A (PKA) molecule. Protein attachment is accomplished by functionalizing a SWCNT-FET device with a single protein and measuring the conductance versus time through the device as it is submerged in an electrolyte solution. PKA-functionalized nanocircuits elucidated the time PKA spent traveling between three different conformational motions upon binding of its two substrates.To generalize this approach for the study of a wide variety of proteins at the single-molecule level, this dissertation investigates the bioconjugation process to determine and isolate the key parameters required for functionalizing a SWCNT with a single protein. Further analysis into the parameters implicated in the thiol-maleimide bioconjugation step of the SWCNT-FET device fabrication proved an exhausting effort. We developed improved linker molecules, anthracene maleimide, for attachment of three different proteins to the SWCNT and a new derivative of maleimide providing a solution to overcome side reactions associated with the reducing agent, tris(2-carboxyethyl)phosphine (TCEP). Protein purity and presence of detergent proved to play key roles in preventing nanocircuit surface fouling. An alternative bioconjugation method, utilizing an azide-functionalized hen egg-white lysozyme (HEWL) to tether the enzyme to the SWCNT through an alkyne linker molecule was developed. The work described here explores the current SWCNT attachment method as well as a new strategy to apply SWCNT-FET devices to study novel proteins. The results demonstrate SWCNT-FET as a sensitive technique for studying conformational dynamics of biological molecules in the microsecond range.
Author: Allan Svendsen
Publisher: CRC Press
ISBN: 9814669334
Category : Mathematics
Languages : en
Pages : 884
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Book Description
Understanding Enzymes: Function, Design, Engineering, and Analysis focuses on the understanding of enzyme function and optimization gained in the past decade, past enzyme function analysis, enzyme engineering, and growing insights from the simulation work and nanotechnology measurement of enzymes in action in vitro or in silico. The book also prese
Author: Robert Peach
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Book Description
Author: Tamiki Komatsuzaki
Publisher: John Wiley & Sons
ISBN: 111813138X
Category : Science
Languages : en
Pages : 403
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Book Description
Discover the experimental and theoretical developments in optical single-molecule spectroscopy that are changing the ways we think about molecules and atoms The Advances in Chemical Physics series provides the chemical physics field with a forum for critical, authoritative evaluations of advances in every area of the discipline. This latest volume explores the advent of optical single-molecule spectroscopy, and how atomic force microscopy has empowered novel experiments on individual biomolecules, opening up new frontiers in molecular and cell biology and leading to new theoretical approaches and insights. Organized into two parts—one experimental, the other theoretical—this volume explores advances across the field of single-molecule biophysics, presenting new perspectives on the theoretical properties of atoms and molecules. Single-molecule experiments have provided fresh perspectives on questions such as how proteins fold to specific conformations from highly heterogeneous structures, how signal transductions take place on the molecular level, and how proteins behave in membranes and living cells.This volume is designed to further contribute to the rapid development of single-molecule biophysics research. Filled with cutting-edge research reported in a cohesive manner not found elsewhere in the literature, each volume of the Advances in Chemical Physics series serves as the perfect supplement to any advanced graduate class devoted to the study of chemical physics.
Author: Benedikt Sohmen
Publisher:
ISBN:
Category :
Languages : en
Pages : 0
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Book Description
Author: Michael V. Mirkin
Publisher: CRC Press
ISBN: 146656122X
Category : Science
Languages : en
Pages : 852
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Book Description
Nanoscale electrochemistry has revolutionized electrochemical research and technologies and has impacted other fields, including nanotechnology and nanoscience, biology, and materials chemistry. This book examines well-established concepts and principles and provides an updated overview of the field and its applications. The first two chapters contain theoretical background, specifically, theories of electron transfer, transport, and double-layer processes at nanoscale electrochemical interfaces. The next chapters examine the electrochemical studies of nanomaterials and nanosystems, as well as the applications of nanoelectrochemical techniques. Each chapter can be read independently, providing readers with a compact, up-to-date review of th
Author: Jianyong Tang
Publisher:
ISBN:
Category :
Languages : en
Pages : 211
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Book Description
Author: Goubin Luo
Publisher:
ISBN:
Category : Proteins
Languages : en
Pages : 424
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Book Description
Author: Dmytro Rodnin
Publisher:
ISBN:
Category :
Languages : en
Pages : 0
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Book Description
Author: Xuefeng Guo
Publisher: John Wiley & Sons
ISBN: 3527818901
Category : Technology & Engineering
Languages : en
Pages : 408
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Book Description
Provides in-depth knowledge on molecular electronics and emphasizes the techniques for designing molecular junctions with controlled functionalities This comprehensive book covers the major advances with the most general applicability in the field of molecular electronic devices. It emphasizes new insights into the development of efficient platform methodologies for building such reliable devices with desired functionalities through the combination of programmed bottom-up self-assembly and sophisticated top-down device fabrication. It also helps to develop an understanding of the device fabrication processes and the characteristics of the resulting electrode-molecule interface. Beginning with an introduction to the subject, Molecular-Scale Electronics: Concept, Fabrication and Applications offers full chapter coverage on topics such as: Metal Electrodes for Molecular Electronics; Carbon Electrodes for Molecular Electronics; Other Electrodes for Molecular Electronics; Novel Phenomena in Single-Molecule Junctions; and Supramolecular Interactions in Single-Molecule Junctions. Other chapters discuss Theoretical Aspects for Electron Transport through Molecular Junctions; Characterization Techniques for Molecular Electronics; and Integrating Molecular Functionalities into Electrical Circuits. The book finishes with a summary of the primary challenges facing the field and offers an outlook at its future. * Summarizes a number of different approaches for forming molecular-scale junctions and discusses various experimental techniques for examining these nanoscale circuits in detail * Gives overview of characterization techniques and theoretical simulations for molecular electronics * Highlights the major contributions and new concepts of integrating molecular functionalities into electrical circuits * Provides a critical discussion of limitations and main challenges that still exist for the development of molecular electronics * Suited for readers studying or doing research in the broad fields of Nano/molecular electronics and other device-related fields Molecular-Scale Electronics is an excellent book for materials scientists, electrochemists, electronics engineers, physical chemists, polymer chemists, and solid-state chemists. It will also benefit physicists, semiconductor physicists, engineering scientists, and surface chemists.
