Author: Jose Freixas Coutin
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Book Description
Postharvest-related seed coat darkening is a major problem in common bean (Phaseolus vulgaris L.), including cranberry bean, as these seeds are graded lower in the commercial market. Proanthocyanidins accumulate in the seed coats of dicotyledonous species, including legumes and their oxidation to quinones promotes seed coat darkening. The upregulated expression of genes encoding late steps in proanthocyanidin biosynthesis (e.g., ANTHOCYANIDIN REDUCTASE, ANR), are associated with seed coat darkening in Medicago truncatula and Arabidopsis thaliana. In this thesis, total proanthocyanidin levels in seed coats increased with seed maturation in a darkening-susceptible cranberry bean recombinant inbred line (RIL), whereas these compounds were undetectable in non-darkening RIL seed coats. RNA sequencing was implemented to investigate transcript changes in darkening and non-darkening RIL seed coats as a function of bean development. The transcript levels for 1336 genes were varied between darkening and non-darkening RIL seed coats at one or more stages of seed development. A model clustering analysis revealed the differentially expressed genes formed 14 gene clusters based on their mode of expression. Structural and regulatory genes of the proanthocyanidin biosynthesis pathway were prevalent in cluster 2 and upregulated in seed coats of the darkening RIL. This included myeloblastosis proto-oncogene (MYB) transcription factors with phylogenetic similarity to proanthocyanidin activating MYBs from other plant species. Moreover, regions upstream of the transcription start site in cluster 2 genes were enriched in MYB and basic helix-loop-helix consensus binding sites as revealed by a transcription factor binding site enrichment analysis. A principal component analysis revealed that transcript abundance of two genes of unknown function and three proanthocyanidin biosynthesis genes, FLAVANONE 3-HYDROXYLASE 1, DIHYDROFLAVONOL 4-REDUCTASE 1 and PvANR1 were highly associated with seed coat proanthocyanidin accumulation in the darkening cranberry bean RIL. Biochemical characterization of a recombinant PvANR1 was performed following the removal of the hexahistidine-tag. PvANR1 catalyzed the NADPH-dependent reduction of cyanidin to catechin and epicatechin in vitro; moreover, high cyanidin concentrations inhibited the formation of both of these products. This study provides biochemical and molecular information for traditional breeding strategies aimed at preserving the aesthetic quality of edible dry beans during postharvest storage.
Author: Muhammad Siddiq
Publisher: John Wiley & Sons
ISBN: 1118448286
Category : Technology & Engineering
Languages : en
Pages : 452
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Book Description
The common beans and pulses are diverse food resources of high nutritional value (protein, energy, fiber and vitamins and minerals) with broad social acceptance. These legume crops demonstrate global adaptability, genotypic and phenotypic diversity, and multiple means of preparation and dietary use. Beans and pulses are produced in regions as diverse as Latin America, Africa, Asia, and North America, and on a scale similar to some other crops, such as wheat, corn, rice and soybeans. Numerous factors influence utilization, including bean type and cultivar selection, cropping environment and systems, storage conditions and handling infrastructure, processing and final product preparation. Nutrient content and bio-availability are dramatically influenced by these conditions. In recent years, beans and pulses have been cited for imparting specific positive health potentiating responses, such as hypocholesteremic response, mitigation of diabetes and colonic cancer, and weight control. Enhanced dry bean utilization focused on improved dietary health is an opportunity within both subsistent and developed populations. This book provides a contemporary source of information that brings together current knowledge and practices in the value chain of beans/pulses production, processing, and nutrition. It provides in-depth coverage of a wide variety of pertinent topics including: breeding, postharvest technologies, composition, processing technologies, food safety, quality, nutrition, and significance in human health. An experienced team of over 25 contributors from North America, Asia, and Africa has written 15 chapters, divided into three sections: Overview, production and postharvest technologies of beans and pulses Composition, value-added processing and quality Culinology, nutrition, and significance in human health Contributors come from a field of diverse disciplines, including crop sciences, food science and technology, food biochemistry, food engineering, nutritional sciences, and culinology. Dry Beans and Pulses Production, Processing and Nutrition is an essential resource for scientists, processors and nutritionists, whatever the work setting.
Author: Roberto Tuberosa
Publisher: Springer Science & Business Media
ISBN: 9400775725
Category : Science
Languages : en
Pages : 711
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Book Description
Our lives and well being intimately depend on the exploitation of the plant genetic resources available to our breeding programs. Therefore, more extensive exploration and effective exploitation of plant genetic resources are essential prerequisites for the release of improved cultivars. Accordingly, the remarkable progress in genomics approaches and more recently in sequencing and bioinformatics offers unprecedented opportunities for mining germplasm collections, mapping and cloning loci of interest, identifying novel alleles and deploying them for breeding purposes. This book collects 48 highly interdisciplinary articles describing how genomics improves our capacity to characterize and harness natural and artificially induced variation in order to boost crop productivity and provide consumers with high-quality food. This book will be an invaluable reference for all those interested in managing, mining and harnessing the genetic richness of plant genetic resources.
Author: Rodomiro Ortiz Ríos
Publisher: Springer
ISBN: 3319205323
Category : Science
Languages : en
Pages : 250
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Book Description
The field of plant breeding has grown rapidly in the last decade with breakthrough research in genetics and genomics, inbred development, population improvement, hybrids, clones, self-pollinated crops, polyploidy, transgenic breeding and more. This book discusses the latest developments in all these areas but explores the next generation of needs and discoveries including omics beyond genomics, cultivar seeds and intellectual and property rights. This book is a leading-edge publication of the latest results and forecasts important areas of future needs and applications.
Author: Ray Rose
Publisher: Humana Press
ISBN: 9781627036122
Category : Science
Languages : en
Pages : 0
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Book Description
Featuring current resources used to discover new legume family genes and to understand genes and their interactions, Legume Genomics: Methods and Protocols provides techniques from expert researchers to study these plants that are so vitally important for food, feed, human nutrition, bioenergy, and industrial purposes. This detailed volume covers genome characterization and analysis, transcriptome analysis and miRNA identification/analysis, forward and reverse genetics, molecular markers, as well as transformation strategies used to investigate gene function and many other topics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and useful, Legume Genomics: Methods and Protocols aims to serve plant molecular biologists, molecular breeders, plant physiologists and biochemists, developmental biologists, and those interested in plant-microbe interactions.
Author: Food and Agriculture Organization of the United Nations
Publisher: Food & Agriculture Org.
ISBN: 9251094632
Category : Cooking
Languages : en
Pages : 196
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Book Description
The aim of raising global awareness on the multitude of benefits of pulses was integral to the International Year of Pulses. This coffee table book is part guide and part cookbook— informative without being technical. The book begins by giving an overview of pulses, and explains why they are an important food for the future. It also has more than 30 recipes prepared by some of the most prestigious chefs in the world and is peppered with infographics. Part I gives an overview of pulses and gives a brief guide to the main varieties in the world. Part II explains step-by-step how to cook them, what to keep in mind and what condiments and instruments to use. Part III underscores the five messages that FAO conveys to the world about the impact pulses have on nutrition, health, climate change, biodiversity and food security. Part IV illustrates how pulses can be grown in a garden patch with easy gardening instructions and how they are grown in the world, highlighting major world producers, importers and exporters. Part V takes the reader on a journey around the world showing how pulses fit a region’s history and culture and visits 10 internationally acclaimed chefs as they go the market to buy pulses. Back at their restaurant or home, each chef prepares easy dishes and gives their best kept secrets. Each chef provides 3 recipes that are beautifully illustrated.
Author: James Barber
Publisher: World Scientific
ISBN: 9813230312
Category : Science
Languages : en
Pages : 367
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Book Description
This book is a tribute to three outstanding scientists, Professors Jan Anderson FRS, Leslie Dutton FRS and John Walker FRS, Nobel Laureate. Covering some of the most recent advances in the fields of Bioenergetics and Photosynthesis, this book is a compilation of contributions from leading scientists actively involved in understanding the natural biological processes associated with the flow of energy in biological cells. The lectures found in this significant volume were presented at a meeting in March 2016 in Singapore to commemorate the outstanding research in this area.The contents begin with the ideas, specially the contribution from Nobel Laureate Rudolph Marcus, who is well-known for creating the theory of electron transport reactions. This is followed by contributions of many others on various aspects of respiratory and photosynthetic transport chains as well as the dynamic regulation of light harvesting and electron transport events in oxygenic photosynthesis. The book is highly recommended to postgraduate students and researchers who are interested in various aspects of bioenergetic cycles.
Author: T. K. Lim
Publisher: Springer Science & Business Media
ISBN: 9400740530
Category : Science
Languages : en
Pages : 1037
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Book Description
This book continues as volume 4 of a multi-compendium on Edible Medicinal and Non-Medicinal Plants. It covers edible fruits/seeds used fresh or processed, as vegetables, spices, stimulants, edible oils and beverages. It encompasses selected species from the following families: Fagaceae, Grossulariaceae, Hypoxidaxeae, Myrsinaceae Olacaceae, Oleaceae, Orchidaceae, Oxalidaceae, Pandanaceae, Passifloraceae, Pedaliaceae, Phyllanthaceae, Pinaceae, Piperaceae, Rosaceae and Rutaceae . This work will be of significant interest to scientists, researchers, medical practitioners, pharmacologists, ethnobotanists, horticulturists, food nutritionists, agriculturists, botanists, conservationists, lecturers, students and the general public. Topics covered include: taxonomy; common/English and vernacular names; origin and distribution; agroecology; edible plant parts and uses; botany; nutritive and pharmacological properties, medicinal uses and research findings; nonedible uses; and selected references.
Author: Chukwuebuka Egbuna
Publisher: CRC Press
ISBN: 0429760337
Category : Health & Fitness
Languages : en
Pages : 658
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Book Description
This first book in this three-volume set provides comprehensive coverage of a wide range of topics in phytochemistry. With chapters from professional specialists from key institutions around the world, the volume starts with an introduction to phytochemistry and details the fundamentals. Part II discusses the state-of-the-art modern methods and techniques in phytochemical research, while Part III provides an informative overview of computational phytochemistry and its applications. Part IV presents novel research findings in the discovery of drugs that will be effective in the treatment of diseases. The chapters are drawn carefully and integrated sequentially to aid flow, consistency, and continuity.
Author: Antonio J. Márquez
Publisher: Springer Science & Business Media
ISBN: 9781402037344
Category : Science
Languages : en
Pages : 416
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Book Description
Legumes are very important plants playing a central role in biological research. They are a key component of sustainable agricultural systems because of symbiotic nitrogen fixation and other beneficial symbiosis with mycorrhizal fungi. Studies on most of the major leguminous crops are hampered by large genome sizes and other disadvantages which have hindered the isolation and characterisation of genes with important roles in legume biology and agriculture. For this reason Lotus japonicus was chosen as a model species for legume research some ten years ago. Since then, many groups around the world have adopted Lotus as a model and have developed numerous resources and protocols to facilitate basic and applied research on this species. This handbook represents the first effort to compile basic descriptions and methods for research in Lotus, including symbiotic processes, cell and molecular biology protocols, functional genomics, mutants, gene tagging and genetic analysis, transformation and reverse genetic analysis, primary and secondary metabolism, and an exhaustive update of the scientific literature available on this plant.
