Tissue-specific Gene Expression in Tomato and Tobacco PDF Download
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Author: Susan Irene Fuerstenberg
Publisher:
ISBN:
Category :
Languages : en
Pages : 380
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Book Description
Author: Susan Irene Fuerstenberg
Publisher:
ISBN:
Category :
Languages : en
Pages : 380
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Book Description
Author: Jong Kuk Na
Publisher:
ISBN:
Category : Inositol
Languages : en
Pages :
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Book Description
Abstract: The objective of this study was to increase the resistance of crops (tobacco and tomato) to water deficit stress. To achieve this goal transgenic plants were generated using two genes, a putative tomato type I inositol 5 polyphosphatase (5PTase) that terminates inositol 1,4,5 trisphosphate (IP3) signaling and ABRE binding factor ABF4 derived from Arabidopsis. Inositol 1,4,5 triphosphate (IP3), as one of phosphoinositides, is known to transduce a stress signal by changing its level in response to water deficit, osmotic stress, and low temperature in plants. Recently it was shown that type I inositol 5 phosphatases (5PTases), At5PTase1 and AtIP5PII/At5PTase2, regulate IP3 level in plant like in animals and that up-regulation of these genes decreases IP3 levels which leads to a reduction in the expression of ABA-/drought-responsive genes in Arabidopsis. On the basis of sequence similarity to Arabidopsis 5PTases, four tomato cDNAs (Le5PT1-4) that encode putative tomato type I 5PTase proteins were identified. Predicted protein sequences of identified Le5PTs had conserved catalytic domains that are required for 5PTase enzyme activity. Two clones, Le5PT1 and Le5PT2 were similar to AtIP5PII/At5PTase2 and At5PTase1, respectively. The expression of Le5PT1 was down-regulated in early time point under dehydration, NaCl, and exogenous ABA treatment, indicating that Le5PT1 may play a negative role in stress signaling. Transgenic tobacco plants with 35S:Le5PT1 did exhibit weak expression of the drought inducible gene, NtERD10B, but did not show correlation with resistance to water deficit stress. AtABF4, a bzip transcription factor, is known to induce the expression of ABA-responsive genes. The expression of Arabidopsis ABF4/AREB2 gene under the control of guard cell specific KST1 promoter was shown to significantly increase drought tolerance in tobacco and tomato plants. The transgenic plants exhibit significantly lower water loss per unit leaf area compared to wild type plants. ABF4-mGFP protein strongly accumulated in guard cell nuclei. The possibility that ABF4 protein can activate 4XKST1-rd29B:mGFP-GUS fusion construct in a water stress dependent manner was tested. The results show that ABF4 itself may not be sufficient to induce GUS expression even under water stress.
Author: Kavita Vasan Karighattam
Publisher:
ISBN:
Category :
Languages : en
Pages : 304
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Book Description
Author: Gregory DeWitt May
Publisher:
ISBN:
Category : Genetic regulation
Languages : en
Pages : 270
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Book Description
Author: Mathilde Causse
Publisher: Springer
ISBN: 3662533898
Category : Science
Languages : en
Pages : 260
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Book Description
This book describes the strategy used for sequencing, assembling and annotating the tomato genome and presents the main characteristics of this sequence with a special focus on repeated sequences and the ancestral polyploidy events. It also includes the chloroplast and mitochondrial genomes. Tomato (Solanum lycopersicum) is a major crop plant as well as a model for fruit development, and the availability of the genome sequence has completely changed the paradigm of the species’ genetics and genomics. The book describes the numerous genetic and genomic resources available, the identified genes and quantitative trait locus (QTL) identified, as well as the strong synteny across Solanaceae species. Lastly, it discusses the consequences of the availability of a high-quality genome sequence of the cultivated species for the research community. It is a valuable resource for students and researchers interested in the genetics and genomics of tomato and Solanaceae.
Author: Hiroshi Ashihara
Publisher: John Wiley & Sons
ISBN: 1119993229
Category : Science
Languages : en
Pages : 424
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Book Description
Various plant metabolites are useful for human life, and the induction and reduction of these metabolites using modern biotechnical technique is of enormous potential important especially in the fields of agriculture and health. Plant Metabolism and Biotechnology describes the biosynthetic pathways of plant metabolites, their function in plants, and some applications for biotechnology. Topics covered include: biosynthesis and metabolism of starch and sugars lipid biosynthesis symbiotic nitrogen fixation sulfur metabolism nucleotide metabolism purine alkaloid metabolism nicotine biosynthesis terpenoid biosynthesis benzylisoquinoline alkaloid biosynthesis monoterpenoid indole alkaloid biosynthesis flavonoid biosynthesis pigment biosynthesis: anthocyanins, betacyanins and carotenoids metabolomics in biotechnology Plant Metabolism and Biotechnology is an essential guide to this important field for researchers and students of biochemistry, plant biology, metabolic engineering, biotechnology, food science, agriculture, and medicine.
Author: K. Lindsey
Publisher: Springer Science & Business Media
ISBN: 9780792311157
Category : Plant cell culture
Languages : en
Pages : 120
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Book Description
Basic techniques - cells tissue culture of model species. Tissue culture & transformation of crop species. Propagation & conservation of germplasm. Direct gene transfer & protoplast fusion. Reproductive tissues. Mutant selection.
Author: Frederick B. Abeles
Publisher: Academic Press
ISBN: 0080916287
Category : Science
Languages : en
Pages : 431
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Book Description
Ethylene in Plant Biology, Second Edition provides a definitive survey of what is currently known about this structurally simplest of all plant growth regulators. This volume contains all new material plus a bibliographic guide to the complete literature of this field. Progress in molecular biology and biotechnology as well as biochemistry, plant physiology, development, regulation, and environmental aspects is covered in nine chapters co-authored by three eminent authorities in plant ethylene research. This volume is the modern text reference for all researchers and students of ethylene in plant and agricultural science. - Completely updated - Concise, readable style for students and professional - Contains an extensive bibliographic guide to the original literature - Well illustrated with diagrams and photographs - Thorough coverage of: ethylene and ethephon roles and effects stress ethylene, biosynthesis of ethylene, molecular biology of ethylene, action of ethylene, agricultural uses of ethylene
Author: Lutz Nover
Publisher: Springer Science & Business Media
ISBN: 3540480374
Category : Science
Languages : en
Pages : 279
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Book Description
The control of plant gene expression at the transcriptional level is the main subject of this volume. Genetics, molecular biology and gene technology have dramatically improved our knowledge of this event. The functional analysis of promoters and transcription factors provides more and more insights into the molecular anatomy of initiation complexes assembled from RNA polymerase and the multiplicity of helper and control proteins. Formation of specific DNA-protein complexes - activating or repressing transcription - is the crux of developmental or environmental control of gene expression. The book presents an up-to-date, critical overview of this rapidly advancing field.
Author: Pravendra Nath
Publisher: CABI
ISBN: 184593962X
Category : Technology & Engineering
Languages : en
Pages : 341
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Book Description
Fruit ripening is an important aspect of fruit production. The timing of it affects supply chains and buying behaviour, and for consumers ripeness not only affects perceptions of health but has nutritional effects too. Ripeness is closely related to spoilage which has a major financial impact on agricultural industries. Currently there are fast moving developments in knowledge of the factors affecting fruit ripeness, and this up-to-date monograph seeks to draw together the disparate research in this area. The aim of the book is to produce a comprehensive account covering almost every area related to fruit ripening including the latest molecular mechanisms regulating fruit ripening, its impact on human nutrition and emerging research and technologies.
