Author: Harlee S. Strauss
Publisher:
ISBN:
Category : DNA.
Languages : en
Pages : 488
Book Description
The Selective Binding of E. Coli RNA Polymerase to Promoters on Phage T7 DNA
Author: Harlee S. Strauss
Publisher:
ISBN:
Category : DNA.
Languages : en
Pages : 488
Book Description
Publisher:
ISBN:
Category : DNA.
Languages : en
Pages : 488
Book Description
The Binding of Escherichia Coli RNA Polymerase to Bacteriophage T7 DNA
Author: Thomas Robert Kadesch
Publisher:
ISBN:
Category :
Languages : en
Pages : 400
Book Description
Publisher:
ISBN:
Category :
Languages : en
Pages : 400
Book Description
Interactions Between Phage T7 Early Promoters and E. Coli RNA Polymerase
Author: Tao-shih Hsieh
Publisher:
ISBN:
Category :
Languages : en
Pages : 200
Book Description
Publisher:
ISBN:
Category :
Languages : en
Pages : 200
Book Description
The Mechanism of Interaction of E. Coli RNA Polymerase with Bacteriophage and Bacterial Promoters
Author: Sigrid Leirmo
Publisher:
ISBN:
Category :
Languages : en
Pages : 324
Book Description
Publisher:
ISBN:
Category :
Languages : en
Pages : 324
Book Description
Kinetic Rate Constants for Binding of E. Coli RNA Polymerase to Early Promoters of T7 DNA
Author: Christopher J. Dayton
Publisher:
ISBN:
Category : Bacteriophages
Languages : en
Pages : 104
Book Description
Publisher:
ISBN:
Category : Bacteriophages
Languages : en
Pages : 104
Book Description
The Interaction of Escherichia Coli RNA Polymerase with Phage [lambda] Promoters
Author: Jung-Hye Roe
Publisher:
ISBN:
Category : Bacteriophage lambda
Languages : en
Pages : 422
Book Description
Publisher:
ISBN:
Category : Bacteriophage lambda
Languages : en
Pages : 422
Book Description
The Interaction of E. Coli RNA Polymerase with Lambda Phage PR Promoter
Author: Won-Chul Suh
Publisher:
ISBN:
Category :
Languages : en
Pages : 430
Book Description
Publisher:
ISBN:
Category :
Languages : en
Pages : 430
Book Description
Molecular Methods for Virus Detection
Author: Danny L. Wiedbrauk
Publisher: Elsevier
ISBN: 0080536891
Category : Science
Languages : en
Pages : 405
Book Description
Molecular diagnostic procedures have been described in a number of recent books and articles. However, these publications have not focused on virus detection, nor have they provided practical protocols for the newer molecular methods. Written by the inventors or principal developers of these technologies, Molecular Methods for Virus Detection provides both reviews of individual methods and instructions for detecting virus nucleic acid sequences in clinical specimens. Each procedure includes quality assurance protocols that are often ignored by other methodology books. Molecular Methods for Virus Detection provides clinically relevant procedures for many of the newer diagnostic methodologies. - Provides state-of-the-art PCR methods for amplification, quantitation, in situ hybridization, and multiplex reactions - Goes beyond PCR with protocols for 3SR, NASBA, LCR, SDA, and LAT - Covers important virus detection methods such as in situ hybridization; Southern, dot, and slot blots; branched chain signal amplification; and chemiluminescence - Includes quality control information crucial in research and clinical laboratories - Most chapters are written by the inventors and principal developers of the methodologies - Includes color plates, 77 figures, and 18 tables
Publisher: Elsevier
ISBN: 0080536891
Category : Science
Languages : en
Pages : 405
Book Description
Molecular diagnostic procedures have been described in a number of recent books and articles. However, these publications have not focused on virus detection, nor have they provided practical protocols for the newer molecular methods. Written by the inventors or principal developers of these technologies, Molecular Methods for Virus Detection provides both reviews of individual methods and instructions for detecting virus nucleic acid sequences in clinical specimens. Each procedure includes quality assurance protocols that are often ignored by other methodology books. Molecular Methods for Virus Detection provides clinically relevant procedures for many of the newer diagnostic methodologies. - Provides state-of-the-art PCR methods for amplification, quantitation, in situ hybridization, and multiplex reactions - Goes beyond PCR with protocols for 3SR, NASBA, LCR, SDA, and LAT - Covers important virus detection methods such as in situ hybridization; Southern, dot, and slot blots; branched chain signal amplification; and chemiluminescence - Includes quality control information crucial in research and clinical laboratories - Most chapters are written by the inventors and principal developers of the methodologies - Includes color plates, 77 figures, and 18 tables
Cloning and Expression of the Gene for Bacteriophage T7 RNA Polymerase
Author:
Publisher:
ISBN:
Category :
Languages : en
Pages :
Book Description
This application describes a means to clone a functional gene for bacteriophage T7 RNA polymerase. Active T7 RNA polymerase is produced from the cloned gene, and a plasmid has been constructed that can produce the active enzyme in large amounts. T7 RNA polymerase transcribes DNA very efficiently and is highly selective for a relatively long promoter sequence. This enzyme is useful for synthesizing large amounts of RNA in vivo or in vitro, and is capable of producing a single RNA selectively from a complex mixture of DNAs. The procedure used to obtain a clone of the T7 RNA polymerase gene can be applied to other T7-like phages to obtain clones that produce RNA polymerases having different promoter specificities, different bacterial hosts, or other desirable properties. T7 RNA polymerase is also used in a system for selective, high-level synthesis of RNAs and proteins in suitable host cells.
Publisher:
ISBN:
Category :
Languages : en
Pages :
Book Description
This application describes a means to clone a functional gene for bacteriophage T7 RNA polymerase. Active T7 RNA polymerase is produced from the cloned gene, and a plasmid has been constructed that can produce the active enzyme in large amounts. T7 RNA polymerase transcribes DNA very efficiently and is highly selective for a relatively long promoter sequence. This enzyme is useful for synthesizing large amounts of RNA in vivo or in vitro, and is capable of producing a single RNA selectively from a complex mixture of DNAs. The procedure used to obtain a clone of the T7 RNA polymerase gene can be applied to other T7-like phages to obtain clones that produce RNA polymerases having different promoter specificities, different bacterial hosts, or other desirable properties. T7 RNA polymerase is also used in a system for selective, high-level synthesis of RNAs and proteins in suitable host cells.
Studies of E. Coli RNA Polymerase-DNA Binding Complexes in the Early Region of
Author: Kathryn Parker
Publisher:
ISBN:
Category : Bacteriophages
Languages : en
Pages : 88
Book Description
Publisher:
ISBN:
Category : Bacteriophages
Languages : en
Pages : 88
Book Description