The Role of RNA-binding Proteins in MRNA Metabolism in Saccharomyces Cerevisiae PDF Download
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Author: Elisa Chao-Fong Shen
Publisher:
ISBN:
Category : Saccharomyces cerevisiae
Languages : en
Pages : 342
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Author: Elisa Chao-Fong Shen
Publisher:
ISBN:
Category : Saccharomyces cerevisiae
Languages : en
Pages : 342
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Book Description
Author: Jeffery Michael Coller
Publisher:
ISBN:
Category :
Languages : en
Pages : 182
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Author: Zdravko Lorkovic
Publisher: CRC Press
ISBN: 149871336X
Category : Science
Languages : en
Pages : 174
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Book Description
Gene expression in eukaryotes is regulated at different levels, which need to be coordinated to implement the information in the genome. Now it is clear that post-transcriptional regulation of gene expression such as pre-mRNA splicing, mRNA transport, editing, turnover and translation are as important as the control of transcription. In all aspects
Author: Joe B. Harford
Publisher: John Wiley & Sons
ISBN: 9780471142065
Category : Science
Languages : en
Pages : 372
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Book Description
mRNA METABOLISM & POST-TRANSCRIPTIONAL GENE REGULATION Edited by Joe B. Harford and David R. Morris Gene expression is a process that begins with the transcription ofDNA to an RNA messenger (mRNA), which is then translated into aprotein. Historically, attention has been focused on the regulationof RNA synthesis (transcription); however, there is a growingrecognition of and appreciation for the importance of the manyregulatory mechanisms that take place after RNA synthesis has beencompleted. mRNA Metabolism and Post-Transcriptional Gene Regulation is thefirst comprehensive overview of the various modes of generegulation that exist post-transcriptionally. Collecting studies bysome of the top researchers in the field, this volume provides bothan up-to-date review of the complex "life" of an mRNA molecule andan introduction to current work on the diversity of mechanisms ofpost-transcriptional reactions. Topics covered include: * RNA structure * Mammalian RNA editing * RNA export from the nucleus * The fundamentals of translation initiation * Control of mRNA decay in plants * mRNA metabolism and cancer * Control of mRNA stability during herpes simplex virus infection * Regulation of mRNA expression in HIV-1 and other complexretroviruses * Nucleases * RNA localization A timely contribution to the understanding of genetic regulatorymechanisms, mRNA Metabolism and Post-Transcriptional GeneRegulation provides a basis from which potential therapeuticstrategies may be developed. This book will be of vital interest tocell and molecular biologists at all levels, from graduate studentsto senior investigators, clinical researchers, and professionals inthe pharmaceutical and biotechnology industries.
Author: Daniel Michael Klass
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Book Description
We are on the threshold of a new era in our understanding of that fantastic feat of regulation at the core of life itself--gene expression. The rapid pace of new developments in genome-wide, high-throughput technologies has allowed us unprecedented access to observe multiple stages of the gene expression program for nearly the entire genome. This has revealed a widespread discordance between mRNA abundance and protein abundance for many genes whose expression changes in response to environmental stimuli, and a significant coordination of post-transcriptional regulation for specific sets of related mRNAs at the levels localization, translation, decay, and the noise in gene expression. Despite this evidence suggesting the existence of a coordinated regulatory framework that potentially affects the fate of every mRNA in the cell, our efforts to discern the underlying structure and regulatory themes are hindered by an incomplete understanding of RNA-protein interactions. To advance our comprehension of post-transcriptional regulation, we developed new tools to identify which proteins bind to RNA, which of those bind concurrently, which RNAs are bound by a given protein, and where each protein binds on each RNA. Using our proteomic tools we discovered hundreds unexpected RNA binding proteins, uncovered new RNA binding domains, identified widespread, concurrent binding with several RNA binding proteins, and inferred functional information from the simultaneous binding partners of several RNA binding proteins. We used our genomic, sequencing-based tools to systematically interrogate a large set of diverse RNA binding proteins and we discerned new themes from the resulting data. This revealed significant differences in function, localization, and regulation among the proteins encoded by the targets of a given RNA binding protein based on binding position. These results suggest that the functional consequences of the RBP-RNA interaction are determined not only by whether an mRNA is bound by an RBP but also by the position of the binding site within the mRNA and its relation to the other RBPs that bind the same mRNA. Overall, we found evidence of an extensive regulatory framework involving hundreds of RNA binding proteins, encompassing nearly the entire transcriptome, and extending our understanding of the RNA-protein interactions at the heart of post-transcriptional regulation.
Author: Christine Elizabeth Brown
Publisher:
ISBN:
Category :
Languages : en
Pages : 376
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Author: Shervin Sean Houshmandi
Publisher:
ISBN:
Category : Electronic dissertations
Languages : en
Pages : 159
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Book Description
The regulation of messenger RNA (mRNA) metabolism is an important step in proper gene expression. In many eukaryotic organisms this regulation can be mediated by a group of highly conserved RNA-binding proteins known as the Puf family of proteins. The yeast Saccharomyces cerevisiae has several proteins that belong to this family. One of the yeast Puf proteins, Puf3p, binds and regulates the COX17 mRNA, which codes for a protein involved in mitochondrial copper transport. Specifically, Puf3p stimulates the decay of COX17 mRNA. However, the precise mechanism of Puf3p binding and decay regulation is yet unknown. The goal of this research is to determine the role of the Puf3p interactions required for regulation of mRNA decay in yeast, and to understand how Puf3p activity is regulated. The studies to examine Puf3p interactions have focused on the Puf3 protein sequences required for specific binding and regulation of COX17 mRNA decay. The data show that a specific region of the Puf3 protein, known as the Puf3 Repeat Domain, is sufficient to both bind COX17 mRNA and also regulate its rate of decay. Furthermore, key amino acids on the RNA-binding surface of the repeat domain that promote target specificity have been identified, as well as a specific loop structure on the protein-binding surface of the repeat domain that is required for RNA decay regulation. In addition, these studies show that the repeat domain of Puf3p directly interacts with other known mRNA decay factors, more specifically, decay factors that are involved in the deadenylation and decapping steps of mRNA decay. Additional collaborative studies have focused on the condition-specific regulation of mRNA stability in yeast. In these studies, the activity of Puf3p was found to be dependent on the available carbon source, as well as inhibited by rapamycin treatment, which in turn places the Puf3p downstream of the Target of Rapamycin (TOR) signaling pathway. Together the results from the research in this body of work will help further our understanding of transcript-specific decay mechanisms in yeast and other eukaryotes.
Author: Kiyoshi Nagai
Publisher: Oxford University Press, USA
ISBN:
Category : Medical
Languages : en
Pages : 302
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Book Description
The study of RNA-protein interactions is crucial to understanding the mechanisms and control of gene expression and protein synthesis. The realization that RNAs are often far more biologically active than was previously appreciated has stimulated a great deal of new research in this field. Uniquely, in this book, the world's leading researchers have collaborated to produce a comprehensive and current review of RNA-protein interactions for all scientists working in this area. Timely, comprehensive, and authoritative, this new Frontiers title will be invaluable for all researchers in molecular biology, biochemistry and structural biology.
Author: Nikoleta Georgieva Tsvetanova
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Book Description
The dynamic processes of a living cell depend on the coordinated temporal and spatial regulation of the many steps of gene expression. Transcription regulation is one control point of gene expression, and a gene can also be regulated post-transcriptionally, by RNA-binding proteins (RBPs). The biological significance of post-transcriptional regulation is especially evident in cases, where RBP binding controls the temporal precision of suppression and activation of important cellular stress responses. We developed a proteome-wide experimental approach for in vitro identification of novel RBPs and RNA-protein interactions in Saccharomyces cerevisiae. We found 12 novel RNA-binding proteins, the majority of which, surprisingly, are currently annotated as enzymes with roles in metabolic processes. We next used this proteomic approach to screen for proteins specifically interacting with the HAC1 RNA, which mediates activation of the yeast unfolded protein response (UPR). We found that HAC1 associated reproducibly with four small yeast GTPases, three of which are of the Ypt family of ras-GTPases. We further characterized one of them, the yeast Rab1 homolog Ypt1, and showed that Ypt1 interacted with unspliced HAC1 RNA only in the absence of ER stress. Selective Ypt1 depletion increased HAC1 RNA stability and expression, and also affected timely recovery from UPR. By developing and applying a novel proteomic approach for studying RNA-protein interactions, we established Ypt1 as an important regulator of HAC1 expression and UPR signaling. This unexpected protein-RNA interaction provides a biochemical mechanism for coordinating the key cellular processes of vesicle trafficking and ER homeostasis.
Author: Amanda J. Macmillan
Publisher:
ISBN:
Category : RNA-protein interactions
Languages : en
Pages : 184
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Book Description
