The Influence of Sterol-inhibiting Fungicides on the Incidence of Grape Powdery Mildew and on Juice Quality PDF Download
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Author: Alice Litton Gittings
Publisher:
ISBN:
Category :
Languages : en
Pages : 156
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Author: Alice Litton Gittings
Publisher:
ISBN:
Category :
Languages : en
Pages : 156
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Author:
Publisher:
ISBN:
Category : Grape industry
Languages : en
Pages : 1006
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Issue no. 12-A, 1983-1990 is a Buyer's guide
Author: Anne M. Morrell
Publisher:
ISBN:
Category : Fruit juice industry
Languages : en
Pages : 86
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Author: Laura Hill Jones
Publisher:
ISBN: 9781321362954
Category :
Languages : en
Pages :
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Powdery mildew, caused by the obligate biotrophic fungus Erysiphe necator, is an economically important disease of grapevines worldwide. Large quantities of fungicides are used for its control, accelerating the incidence of fungicide-resistance. A shotgun approach was applied to sequence and assemble the genome of five E. necator isolates, and RNA-seq and comparative genomics were used to predict and annotate protein-coding genes. The results show that the E. necator genome is exceptionally large and repetitive and suggest that transposable elements are responsible for genome expansion. Frequent structural variations were found between isolates and included copy number variation in EnCYP51, the target of the commonly used sterol demethylase inhibitor (DMI) fungicides. A collection of 89 additional E. necator isolates from diverse vineyard sites was screened for copy number variation in the EnCYP51 gene and for presence/absence of a point mutation (Y136F) known to result in higher fungicide tolerance. The results show that an increase in EnCYP51 copy number is significantly more likely to be detected in isolates collected from fungicide-treated vineyards. Increased EnCYP51 copy numbers were detected with the Y136F allele, suggesting that an increase in copy number becomes advantageous only after the fungicide-tolerant allele is acquired. The results also show that EnCYP51 copy number influences expression in a gene-dose dependent manner and correlates with fungal growth in the presence of a DMI fungicide. Taken together these results demonstrate that copy number variation can be adaptive in the development of resistance to DMI fungicides in E. necator.
Author: Daniel Owen Chellemi
Publisher:
ISBN:
Category :
Languages : en
Pages : 298
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Author: Fred Elmer Gladwin
Publisher:
ISBN:
Category : Agriculture
Languages : en
Pages : 24
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Author: F. E. Gladwin
Publisher:
ISBN:
Category : Downy mildew diseases
Languages : en
Pages : 18
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Author:
Publisher:
ISBN:
Category : Wine and wine making
Languages : en
Pages : 164
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Author:
Publisher:
ISBN:
Category : Viticulture
Languages : en
Pages : 92
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Author: Viticulture Consortium, East
Publisher:
ISBN:
Category : Grapes
Languages : en
Pages : 288
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