Techniques in Quantification and Localization of Gene Expression PDF Download
Are you looking for read ebook online? Search for your book and save it on your Kindle device, PC, phones or tablets. Download Techniques in Quantification and Localization of Gene Expression PDF full book. Access full book title Techniques in Quantification and Localization of Gene Expression by Bruce K. Patterson. Download full books in PDF and EPUB format.
Author: Bruce K. Patterson
Publisher: Springer Science & Business Media
ISBN: 1461213428
Category : Science
Languages : en
Pages : 157
Get Book Here
Book Description
Can the son or daughter of a baseball pitcher or cricket bowler throw a ball 100 miles an hour? Is the son or daughter of an opera singer also an opera singer? Is a house with functional light switches lit? The line of thinking in these rhetorical questions also applies to human genetics. What do baseball pitchers, opera sing ers, light switches, and the Human Genome Project have in common? These questions address the issue of potential versus realization of function. Although sons and daughters of baseball pitchers and opera singers may have inherited the mechanical attributes to be baseball pitchers and opera singers, they may not, at any point in time, be baseball pitchers or opera singers. A house with functional light switches is not lit unless the light switches are on. Similarly, all of the genes discovered and sequenced as a result of the Human Genome Project are not expressed at the same time. Genome project information will allow us to deter mine the repertoire of genes in an individual, which is analogous to determining where the light switches in a house are located and whether they are functional (a mutation or deletion in the Genome Project Model). The pattern of "on" light switches in a house gives us functional information as to what the family inside is doing (e. g. , eating, reading, sleeping). Similarly, the pattern of gene expression (RNA) gives us information on what our bodies are doing (e. g.
Author: Bruce K. Patterson
Publisher: Springer Science & Business Media
ISBN: 1461213428
Category : Science
Languages : en
Pages : 157
Get Book Here
Book Description
Can the son or daughter of a baseball pitcher or cricket bowler throw a ball 100 miles an hour? Is the son or daughter of an opera singer also an opera singer? Is a house with functional light switches lit? The line of thinking in these rhetorical questions also applies to human genetics. What do baseball pitchers, opera sing ers, light switches, and the Human Genome Project have in common? These questions address the issue of potential versus realization of function. Although sons and daughters of baseball pitchers and opera singers may have inherited the mechanical attributes to be baseball pitchers and opera singers, they may not, at any point in time, be baseball pitchers or opera singers. A house with functional light switches is not lit unless the light switches are on. Similarly, all of the genes discovered and sequenced as a result of the Human Genome Project are not expressed at the same time. Genome project information will allow us to deter mine the repertoire of genes in an individual, which is analogous to determining where the light switches in a house are located and whether they are functional (a mutation or deletion in the Genome Project Model). The pattern of "on" light switches in a house gives us functional information as to what the family inside is doing (e. g. , eating, reading, sleeping). Similarly, the pattern of gene expression (RNA) gives us information on what our bodies are doing (e. g.
Author: Ho Suk Lee
Publisher:
ISBN: 9781321837162
Category :
Languages : en
Pages : 114
Get Book Here
Book Description
A technology for biomolecular processing and localization of gene expression at the single cell level is essential to understanding cellular heterogeneity. Traditional methods of tissue analysis cannot identify the stochastic variation that makes individual cells unique because these differences are masked by bulk measurements. In this dissertation, two technologies are described for different aspects of single cell analysis. The first part is a sample preparation method for biomolecular processing from single cell using advanced microfluidic devices with permeable polymer barriers; the second one is digital quantification and localization of biomolecules at the single cell level. Microfluidic devices provide a powerful tool for cells and biomolecular processing due to their ability to compartmentalize reactions at physiologically relevant concentrations in a highly parallelizable manner. However, the serial biochemical reaction required for efficient and low-loss single-cell analysis cannot be performed in a single fluidic chamber. In the first part of the dissertation a novel microfluidic architecture is described that uses semipermeable polymer barriers to enables the capture and transportation of DNA and cells, solution exchange, and serial biochemical reactions to be carried out in a single microfluidic chamber. Meanwhile, advancements in single-cell sequencing techniques have allowed the full gene expression of single cell to be profiled. However, the native spatial context of the cell is lost because of the process of cell lysis. In the second part of the dissertation, a highly multiplexed method to selectively detect and localize hundreds of targeted genes in-situ is presented. This method retains local information of targeted genes by processing enzymatic reactions in-situ without destroying cells. It also utilizes the multiplexing capability and high specificity of padlock probes to detect targets of interest at a high signal-to-noise ratio by selectively amplifying biomolecules of interest.
Author: Kåre Lehmann Nielsen
Publisher: Springer Science & Business Media
ISBN: 1588296768
Category : Medical
Languages : en
Pages : 213
Get Book Here
Book Description
Serial Analysis of Gene Expression (SAGE): Digital Gene Expression Profiling facilitates the introduction of SAGE into the laboratory, and provides a framework for interpreting and comparing data derived from SAGE experiments. Of the several methods of genetic profiling available, only SAGE measures the expression of both known and unknown genes. SAGE studies encompass 50,000 tags and can provide detailed knowledge of the 2000 most highly expressed genes in the tissue sample. The SAGE protocols presented are detailed, fully annotated, and tested, and are all written by experienced SAGE researchers from around the world. Part 1 is dedicated to experimental procedures of SAGE and related methods including aRNA LongSAGE, SuperSAGE, DeepSAGE, and GMAT. Part 2 provides methods for extraction and filtration of tags, analysis of ditag populations, and completing statistically correct comparisons of gene expression profiles. Comparative transcriptomics enables scientists to understand the underlying genetics of biological changes such as development, disease, crop yield, and resistance. SAGE analysis is also used to obtain unknown tags, which can be used as gene-specific primers in Rapid Amplification of cDNA Ends (RACE) reactions to generate full-length transcripts for cloning and sequencing. This book will be an indispensable tool for any lab engaged in genetic profiling and comparative transcriptomics, and will help many laboratories to successfully implement tag-based sequencing methods and procedures and obtain comprehensive, useful, and interpretative data.
Author: Melody Clark
Publisher: Wiley-Blackwell
ISBN: 9783527308859
Category : Science
Languages : de
Pages : 0
Get Book Here
Book Description
In situ hybridization is a proven, powerful technique with applications in chromosome and genome analysis, as well as gene expression. Covering a carefully selected range of techniques with immediate and general applications in research and clinical diagnosis, the book starts with genome and DNA mapping, continues through gene expression localization in wholemount and tissue sections, and on to ultrastructural levels. The step-by-step protocols used reflect research in these areas and are all reproducible.
Author:
Publisher:
ISBN: 9780815332183
Category : Cells
Languages : en
Pages : 0
Get Book Here
Book Description
Author: Dr. Joe O'Connell
Publisher:
ISBN: 9780896038752
Category : Molecular biology
Languages : en
Pages : 378
Get Book Here
Book Description
Author:
Publisher:
ISBN:
Category :
Languages : en
Pages : 33
Get Book Here
Book Description
The main objectives of this Program Project is to develop strategy and technology for the study of gene structure, organization and function in a multi-disciplinary, highly coordinated manner. In Project I, Molecular Cytology, the establishment of all instrumentation for the computerized microscopic imaging system (CMIS) has been completed with the software in place, including measurement of the third dimension (along the Z-axis). The technique is now at hand to measure single copy DNA in the nucleus, single copy mRNA in the cell, and finally, we are in the process of developing mathematical approaches for the analysis of the relative spatial 3-D relationship among the chromosomes and the individual genes in the interphasal nucleus. Also, we have a sensitive and reliable method for measuring single-stranded DNA breaks which will be useful for the determination of damage to DNA caused by ionizing radiation. In Project II, the mapping of restriction fragments by 2-D enzymatic and electrophoretic analysis has been perfected for application. In Project III, a major finding is that the binding constant and effectiveness of antisense oligonucleotide analogues, Matagen, can be significantly improved by substituting 2(prime)-O-methylribos methylphosphonate backbones for the current 2(prime)-deoxyribomethylphosphonate backbones. 15 refs., 10 figs., 2 tabs.
Author: Leandro Peña
Publisher: Springer Science & Business Media
ISBN: 1592598277
Category : Science
Languages : en
Pages : 427
Get Book Here
Book Description
The aim of Transgenic Plants: Methods and Protocols is to provide a source of information to guide the reader through a wide range of frequently used, broadly applicable, and easily reproducible techniques involved in the gene- tion of transgenic plants. Its step-by-step approach covers a series of methods for genetically transforming plant cells and tissues, and for recovering whole transgenic plants from them. The volume then moves on to the use of sele- able and reporter markers, positive selection, marker elimination after rec- ery of transgenic plants, and the analysis of transgene integration, expression, and localization in the plant genome. Although contributors usually refer to model plants in most chapters, the protocols described herein should be widely applicable to many plant species. The last two sections are devoted to me- ods of risk assessment and to exploring the current and future applications of transgenic technology in agriculture and its social implications in a case study. Transgenic Plants: Methods and Protocols is divided into six major s- tions plus an introduction, comprising 27 chapters. Part I, the Introduction, is a review of the past, present, and perspectives of the transgenic plants, from the discovery of Agrobacterium tumefaciens as a feasible transformation vector, to its use as a tool to study gene expression and function, and the current and possible future applications of this technology in agriculture, industry, and medicine.
Author: Lawrence Baker
Publisher:
ISBN: 9781641160032
Category : Science
Languages : en
Pages : 238
Get Book Here
Book Description
Bioinformatics is a field which uses the principles and techniques of mathematics, computer science, engineering, statistics, etc. to analyse and interpret biological data. It is also used to understand the complex system of proteomics. The topics studied under this area are DNA sequencing, computational evolutionary biology, pan genomics, gene expression analysis, protein localization, structural bioinformatics, biodiversity informatics, etc. This book unfolds the innovative aspects of bioinformatics which will be crucial for the holistic understanding of the subject matter. It studies, analyses and upholds the pillars of this field and its utmost significance in modern times. This textbook will serve as a reference to a broad spectrum of readers.
Author: Robert D. Goldman
Publisher: CSHL Press
ISBN: 9780879696832
Category : Science
Languages : en
Pages : 658
Get Book Here
Book Description
Recent advances in imaging technology reveal, in real time and great detail, critical changes in living cells and organisms. This manual is a compendium of emerging techniques, organized into two parts: specific methods such as fluorescent labeling, and delivery and detection of labeled molecules in cells; and experimental approaches ranging from the detection of single molecules to the study of dynamic processes in organelles, organs, and whole animals. Although presented primarily as a laboratory manual, the book includes introductory and background material and could be used as a textbook in advanced courses. It also includes a DVD containing movies of living cells in action, created by investigators using the imaging techniques discussed in the book. The editors, David Spector and Robert Goldman, whose previous book was Cells: A Laboratory Manual,are highly respected investigators who have taught microscopy courses at Cold Spring Harbor Laboratory, the Marine Biology Laboratory at Woods Hole, and Northwestern University.
