Author: Ruth Hogue Angeletti
Publisher: Academic Press
ISBN: 1483259463
Category : Science
Languages : en
Pages : 563
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Book Description
Techniques in Protein Chemistry III compiles papers presented at the Fifth Protein Society Symposium in Baltimore on June 22-26, 1991. This book discusses the protein and peptide recovery from PVDF membranes; high-sensitivity peptide mapping utilizing reversed-phase microbore and microcolumn liquid chromatography; and capillary electrophoresis for preparation of peptides and direct determination of amino acids. The TFMSA/TFA cleavage in t-Boc peptide synthesis; applications of automatic PTC amino acid analysis; and identification of O-glycosylation sites with a gas phase sequencer are also elaborated. This text likewise covers the conformational stability of the molten globule of cytochrome c and role of aqueous solvation in protein folding. This publication is useful to students and researchers interested in methods and research approaches on protein chemistry.
Author:
Publisher: Elsevier
ISBN: 008054228X
Category : Science
Languages : en
Pages : 933
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Book Description
Praise for the Series:"The mainly sharp scientific focus of this set of snapshots is a credit to both the contricutors and the editorial team."--Biotechnology and Applied Biochemistry Techniques in Protein Chemistry VIII is the latest volume in this successful series. As a valuable bench-top reference tool for protein chemists, the ten section sof the book are divided by subject area to show the reader which techniques are currently applied to particular problems in protein science. This approach reflects current trends in which specific instruments and methodologies are used in several different areas. * * The book features the latest advances in protein chemistry methodologies in the following areas:* Protein sequencing and amino acid analysis* Mass spectral analysis of peptides and proteins* Posttranslational processing* High-sensitivity protein and peptide separations* Protein folding and NMR* Functional domain analysis* Protein design and engineering* Three-dimensional protein structure
Author: Ruth Hogue Angeletti
Publisher: Academic Press
ISBN: 1483268225
Category : Science
Languages : en
Pages : 661
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Book Description
Techniques in Protein Chemistry IV compiles papers presented at the Sixth Protein Society Symposium held in San Diego, California in 1992. This book discusses the mass spectrometry in protein sequence and structural investigations; site specific heterogeneity of N-linked oligosaccharides on recombinant human erythropoietin; and modification of thiophosphorylated proteins with extrinsic probes. The cysteine and tryptophan amino acid analysis of ABRF92-AAA; development of separation strategies for proteins by capillary electrophoresis; and peptide mapping of 2-D gel proteins by capillary HPLC are also elaborated. This text likewise covers the single syringe-pump solid-phase protein sequencer; hydrophobic contact density distribution functions; and application of chemical shift calculation to protein structure determination by NMR. This publication is valuable to biologists and students concerned with the developments in mass spectrometry of proteins.
Author:
Publisher: Elsevier
ISBN: 9780080542263
Category : Science
Languages : en
Pages : 585
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Book Description
Techniques in Protein Chemistry VI, an invaluable bench-top reference source for protein chemists, highlights current methods in the following areas: Protein sequencing and amino acid analysis Mass spectral analysis of peptides and proteins Posttranslational processing High-sensitivity protein and peptide separations Protein folding and NMR Analysis of protein interactions Protein design and engineering Techniques in Protein Chemistry VI, an invaluable bench-top reference source for protein chemists, highlights current methods in the following areas: Protein sequencing and amino acid analysis Mass spectral analysis of peptides and proteins Posttranslational processing High-sensitivity protein and peptide separations Protein folding and NMR Analysis of protein interactions Protein design and engineering
Author: Tony Hugli
Publisher: Elsevier
ISBN: 032314425X
Category : Science
Languages : en
Pages : 631
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Book Description
Techniques in Protein Chemistry compiles reports of methods and techniques presented at the second symposium of the Protein Society in August 1988. This book includes methods and applications in protein sequencing, advanced applications of mass spectrometry and nuclear magnetic resonance technology, limitations of amino acid microanalysis, and advances in high-performance liquid chromatography. The structure of synthetic test peptide-3 (STP-3), a peptide designed to test the analytical limits of current technology in the field of protein chemistry is also elaborated. This publication is suited for chemists and researchers conducting work on the analytical techniques available for the molecular characterization of proteins.
Author: John W. Crabb
Publisher: Elsevier
ISBN: 1483217590
Category : Science
Languages : en
Pages : 591
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Book Description
Techniques in Protein Chemistry V highlights current methods in peptide and protein mass spectrometry, sequence and amino acid analysis, fragmentations, separations, protein folding and modeling, peptide and protein NMR, and peptide synthesis. This volume emerged from the manuscripts presented at the Seventh Symposium of the Protein Society, held in San Diego on July 24-28, 1993. This volume is organized into eight parts encompassing 61 chapters. The first part surveys the peptide and protein characterization, detection, and analysis by mass spectrometry. The subsequent parts describe the structural characterization and analysis of posttranslational processing events, as well as the characterization of protein and amino acid sequences using several analytical techniques. Other parts explore other analytical methods for peptide and protein separations; some aspects involved in protein design and functional domain analysis; and the evaluation of protein conformation, folding, and modeling. The last parts contain research papers on NMR analysis of peptide and protein solution structures. These parts also look into topics related to peptide synthesis and peptide libraries. This book is intended primarily for protein and analytical chemists.
Author: Villafranc
Publisher: Elsevier
ISBN: 0323139442
Category : Science
Languages : en
Pages : 635
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Book Description
Current Research in Protein Chemistry: Techniques, Structure, and Function focuses on the techniques and methods used for determining the structure and function of proteins. Topics covered range from protein folding and stability to catalysis by chimeric proteins, amino acid and peptide analysis, applications of mass spectrometry to peptide and protein analysis, and protein sequencing. This book is divided into six sections encompassing 55 chapters. The first chapter describes a novel method for protein hydrolysis by means of microwave irradiation that uses Teflon-Pyrex tubes. This is followed by a discussion of the application of high performance capillary electrophoresis to the analysis of amino acids. The sections that follow focus on mass spectrometric methods, protein sequencing, and capillary electrophoresis as well as protein stability, chimeric proteins and enzyme modifications, and protein structure prediction. The crystal structure of human interleukin-1alpha, the acid-denatured states of proteins, solubility of recombinant proteins expressed in Escherichia coli, and catalysis by chimeric proteins are considered. The reader is also introduced to peptide mapping and internal sequencing of proteins from acrylamide gels, new approaches to covalent sequence analysis, alkaline denaturation of hemoglobin, and measurements of disulfide bond stabilities in protein folding intermediates. Students and researchers interested in protein chemistry will find this book extremely helpful.
Author: Tony E. Hugli
Publisher:
ISBN: 9780126820010
Category : Amino acid sequence
Languages : en
Pages : 636
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Book Description
Author: A. Darbre
Publisher:
ISBN:
Category : Science
Languages : en
Pages : 650
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Book Description
Protein chemistry is a wide field embracing the analysis, synthesis, sequencing and separation of peptides, proteins and individual amino acids. Practical protein chemistry is a survey of proven techniques designed primarily to be a laboratory handbook for research workers involved with proteins.
Author: Protein Society
Publisher:
ISBN:
Category : Science
Languages : en
Pages : 562
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Book Description
Techniques in Protein Chemistry VII is the latest in a highly successful chronicle of the most recent developments in proteins. The contributions were selected from presentations at the Ninth Symposium of the Protein Society held in Boston, Massachusetts in July 1995.
