Author: Alfons Navarro
Publisher: Humana
ISBN: 9781071615836
Category : Medical
Languages : en
Pages : 0
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Book Description
This volume presents techniques needed for the study of long non-coding RNAs (lncRNAs) in cancer from their identification to functional characterization. Chapters guide readers through identification of lncRNA expression signatures in cancer tissue or liquid biopsies by RNAseq, single Cell RNAseq, Phospho RNAseq or Nanopore Sequencing techniques; validation of lncRNA signatures by Real time PCR, digital PCR or in situ hybridization; and functional analysis by siRNA or CRISPR based methods for lncRNA silencing or overexpression. Lipid based nanoparticles for delivery of siRNAs in vivo, lncRNA-protein interactions, viral lncRNAs and circRNAs are also treated in this volume. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and practical, Long Non-Coding RNAs in Cancer aims to provide a collection of laboratory protocols, bioinformatic pipelines, and review chapters to further research in this vital field.
Author: Hui Ling
Publisher: Elsevier Inc. Chapters
ISBN: 0128061227
Category : Medical
Languages : en
Pages : 36
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Book Description
The discovery of microRNA (miRNA) involvement in cancer a decade ago, and the more recent findings of long non-coding RNAs in human diseases, challenged the long-standing view that RNAs without protein-coding potential are simply “junk” transcription within the human genome. These findings evidently changed the dogma that “DNA makes RNA makes protein” by showing that RNAs themselves can be essential regulators of cellular function and play key roles in cancer development. MiRNAs are evolutionarily conserved short single-stranded transcripts of 19–24 nucleotides in length. They do not code for proteins, but change the final output of protein-coding genes by regulating their transcriptional and/or translation process. Ultraconserved genes (UCGs) are non-coding RNAs with longer length (>200bp) that are transcribed from the ultraconserved genomic region. Both miRNAs and UCGs are located within cancer-associated genomic regions (CAGRs) and can act as tumor suppressors or oncogenes. In this chapter, we present principles and concepts that have been identified over the last decade with respect to our understanding of the function of non-coding RNAs, and summarize recent findings on the role of miRNAs and UCGs in cancer development. Finally, we will conclude by discussing the translational potential of this knowledge into clinical settings such as cancer diagnosis, prognosis and treatment.
Author: Muller Fabbri
Publisher: Springer Science & Business Media
ISBN: 1461484448
Category : Medical
Languages : en
Pages : 287
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Book Description
The discovery of microRNAs and its role as gene expression regulators in human carcinogenesis represents one of the most important scientific achievements of the last decade. More recently, other non-coding RNAs have been discovered and its implications in cancer are emerging as well, suggesting a broader than anticipated involvement of the non-coding genome in cancer. Moreover, completely new and unexpected functions for microRNAs are being revealed, leading to the identification of new anticancer molecular targets. This book represents a comprehensive guide on non-coding RNAs and cancer, spanning from its role as cancer biomarkers, to providing the most useful bioinformatic tools, to presenting some of the most relevant discoveries, which indicates how these fascinating molecules act as fine orchestrators of cancer biology.
Author: M.R.S. Rao
Publisher: Springer
ISBN: 9811052034
Category : Medical
Languages : en
Pages : 336
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Book Description
This contributed volume offers a comprehensive and detailed overview of the various aspects of long non-coding RNAs and discusses their emerging significance. Written by leading experts in the field, it motivates young researchers around the globe, and offers graduate and postgraduate students fascinating insights into genes and their regulation in eukaryotes and higher organisms.
Author: Cesar Lopez-Camarillo
Publisher: CRC Press
ISBN: 1466576774
Category : Medical
Languages : en
Pages : 426
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Book Description
MicroRNA (miRNA) biology is a cutting-edge topic in basic as well as biomedical research. This is a specialized book focusing on the current understanding of the role of miRNAs in the development, progression, invasion, and metastasis of diverse types of cancer. It also reviews their potential for applications in cancer diagnosis, prognosis, and th
Author: Gautam Sethi
Publisher:
ISBN: 9789811267383
Category :
Languages : en
Pages : 0
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Book Description
"Non-coding RNA Transcripts in Cancer Therapy: Pre-clinical and Clinical Implications" provides a new insight towards role of non-coding RNAs molecules including microRNAs, long non-coding RNAs and circular RNAs in cancer. The reason of focusing on cancer is that this malignant disease is responsible for high death around the world and after cardiovascular diseases, it is a leading cause of death. In the current book, the basic information and knowledge about ncRNAs, their biogenesis and their biological functions are covered. It is shown that ncRNAs can regulate expression level of genes at transcriptional and post-transcriptional levels and therefore, ncRNAs are master and key players in molecular pathways. Then, the impact of regulation of molecular pathways by ncRNAs on important molecular mechanisms such as proliferation, metastasis, epithelial-to-mesenchymal transition, apoptosis and autophagy are described. All of these aspects are integrated in cancer and for treatment of this disease. In case of miRNAs, their biogenesis and biological functions in physiological conditions are described. Then, impact of miRNAs on proliferation, metastasis, therapy response (drug resistance and radio-resistance) is described. For lncRNAs and circRNAs, a same way is followed and their biogenesis and functional roles in cells are discussed. At the next step, function of lncRNAs and circRNAs in regulating proliferation, metastasis and therapy response (chemoresistance and radio-resistance) is introduced. These subjects are discussed based on pre-clinical studies and in order to make the book more comprehensive and broaden its scope, the function of exosomal ncRNAs in diagnosis and prognosis of cancer patients is described.
Author: Frank J. Slack
Publisher: World Scientific
ISBN: 1848163665
Category : Science
Languages : en
Pages : 299
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Book Description
MicroRNAs have recently emerged as key regulators of gene expression during development and are frequently misexpressed in human disease states, in particular cancer. These 22-nucleotide-long transcripts act to promote or repress cell proliferation, migration and apoptosis during development, all of which are processes that go awry in cancer. Thus, microRNAs have the ability to behave like oncogenes or tumor suppressors. In addition, their small size and molecular properties make them amenable as targets and therapeutics in cancer treatment. This book goes into detail on how microRNAs represent a paradigm shift in thinking about gene regulation during development and disease, and provide the oncologist with a potentially powerful new battery of agents to diagnose and treat cancer.
Author: Gautam Sethi
Publisher: World Scientific
ISBN: 9811267405
Category : Medical
Languages : en
Pages : 377
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Book Description
'Non-coding RNA Transcripts in Cancer Therapy: Pre-clinical and Clinical Implications' provides a new insight towards role of non-coding RNAs molecules including microRNAs, long non-coding RNAs and circular RNAs in cancer. The reason of focusing on cancer is that this malignant disease is responsible for high death around the world and after cardiovascular diseases, it is a leading cause of death. In the current book, the basic information and knowledge about ncRNAs, their biogenesis and their biological functions are covered. It is shown that ncRNAs can regulate expression level of genes at transcriptional and post-transcriptional levels and therefore, ncRNAs are master and key players in molecular pathways. Then, the impact of regulation of molecular pathways by ncRNAs on important molecular mechanisms such as proliferation, metastasis, epithelial-to-mesenchymal transition, apoptosis and autophagy are described. All of these aspects are integrated in cancer and for treatment of this disease. In case of miRNAs, their biogenesis and biological functions in physiological conditions are described. Then, impact of miRNAs on proliferation, metastasis, therapy response (drug resistance and radio-resistance) is described. For lncRNAs and circRNAs, a same way is followed and their biogenesis and functional roles in cells are discussed. At the next step, function of lncRNAs and circRNAs in regulating proliferation, metastasis and therapy response (chemoresistance and radio-resistance) is introduced. These subjects are discussed based on pre-clinical studies and in order to make the book more comprehensive and broaden its scope, the function of exosomal ncRNAs in diagnosis and prognosis of cancer patients is described.
Author: James C. Johnson
Publisher: Nova Science Publishers
ISBN: 9781626184428
Category : Science
Languages : en
Pages : 0
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Book Description
This book presents current research in the study of new technologies, developments and applications of microRNA and non-coding RNA. Topics include the role of microRNA and short interfering RNAs in plants; microRNAs, major affective disorders and suicidal behavior; microRNA in endoparasites; the functional roles of non-coding RNAs in glioma and their clinical implications; identifying miRNA function in innate immunity; and how multiple IsomiRs and diversity of miRNA sequences unveil evolutionary roles and functional relationships across animals.
Author: Sunil R. Lakhani
Publisher: Springer
ISBN: 149396643X
Category : Medical
Languages : en
Pages : 369
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Book Description
The aim of the book is to discuss the application of molecular pathology in cancer research, and its contribution in the classification of different tumors and identification of potential molecular targets, as well as how this knowledge may be translated into clinical practice, and the huge impact this field is likely to have in the next 5 to 10 years.
