Colorimetric Determination of Nitrate Plus Nitrite in Water by Enzymatic Reduction, Automated Discrete Analyzer Methods PDF Download
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Author: Charles J Patton
Publisher: CreateSpace
ISBN: 9781500223311
Category : Technology & Engineering
Languages : en
Pages : 44
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Book Description
This report documents work at the U.S. Geological Sur- vey (USGS) National Water Quality Laboratory (NWQL) to validate enzymatic reduction, colorimetric determinative meth- ods for nitrate + nitrite in filtered water by automated discrete analysis. In these standard- and low-level methods (USGS I-2547-11 and I-2548-11), nitrate is reduced to nitrite with nontoxic, soluble nitrate reductase rather than toxic, granular, copperized cadmium used in the longstanding USGS auto- mated continuous-flow analyzer methods I-2545-90 (NWQL laboratory code 1975) and I-2546-91 (NWQL laboratory code 1979). Colorimetric reagents used to determine resulting nitrite in aforementioned enzymatic- and cadmium-reduction meth- ods are identical. The enzyme used in these discrete analyzer methods, designated AtNaR2 by its manufacturer, is produced by recombinant expression of the nitrate reductase gene from wall cress (Arabidopsis thaliana) in the yeast Pichia pastoris. Unlike other commercially available nitrate reductases we evaluated, AtNaR2 maintains high activity at 37°C and is not inhibited by high-phenolic-content humic acids at reaction temperatures in the range of 20°C to 37°C. These previously unrecognized AtNaR2 characteristics are essential for success- ful performance of discrete analyzer nitrate + nitrite assays (henceforth, DA-AtNaR2) described here.
Author: Charles J Patton
Publisher: CreateSpace
ISBN: 9781500223311
Category : Technology & Engineering
Languages : en
Pages : 44
Get Book Here
Book Description
This report documents work at the U.S. Geological Sur- vey (USGS) National Water Quality Laboratory (NWQL) to validate enzymatic reduction, colorimetric determinative meth- ods for nitrate + nitrite in filtered water by automated discrete analysis. In these standard- and low-level methods (USGS I-2547-11 and I-2548-11), nitrate is reduced to nitrite with nontoxic, soluble nitrate reductase rather than toxic, granular, copperized cadmium used in the longstanding USGS auto- mated continuous-flow analyzer methods I-2545-90 (NWQL laboratory code 1975) and I-2546-91 (NWQL laboratory code 1979). Colorimetric reagents used to determine resulting nitrite in aforementioned enzymatic- and cadmium-reduction meth- ods are identical. The enzyme used in these discrete analyzer methods, designated AtNaR2 by its manufacturer, is produced by recombinant expression of the nitrate reductase gene from wall cress (Arabidopsis thaliana) in the yeast Pichia pastoris. Unlike other commercially available nitrate reductases we evaluated, AtNaR2 maintains high activity at 37°C and is not inhibited by high-phenolic-content humic acids at reaction temperatures in the range of 20°C to 37°C. These previously unrecognized AtNaR2 characteristics are essential for success- ful performance of discrete analyzer nitrate + nitrite assays (henceforth, DA-AtNaR2) described here.
Author: Patrick John Canney
Publisher:
ISBN:
Category : Nitrates
Languages : en
Pages : 336
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Book Description
Author: Chaudhery Mustansar Hussain
Publisher: Elsevier
ISBN: 0128236973
Category : Science
Languages : en
Pages : 462
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Book Description
Smartphone usage has created a new means for detection, analysis, diagnosis and monitoring through the use of new apps and attachments. These breakthrough analytical methods offer ways to overcome the drawbacks of more conventional methods, such as the expensive instrumentation that is often needed, complex sample pre-treatment steps, or time-consuming procedures. Smartphone-Based Detection Devices: Emerging Trends in Analytical Techniques gathers these modern developments in smartphone analytical methods into one comprehensive source, covering recent advancements in analytical tools while paying special attention to the most accurate, highly efficient approaches. Serving as a guide not only to analytical chemists but also to environmentalists, biotechnologists, pharmacists, forensic scientists and toxicologists, Smartphone-Based Detection Devices: Emerging Trends in Analytical Techniques is an important source for researchers who require accurate analysis of their on- and off-site samples. Students in these fields at the graduate and post-graduate level will also benefit from this topical and comprehensive book. - Provides an integrated approach for advanced analytical methods and techniques using smartphones - Covers the usage of smartphones in sample prep, integration and detection stages of analytical chemistry - Applicable for researchers of all levels, from graduate students to professionals
Author:
Publisher:
ISBN:
Category : Hydrology
Languages : en
Pages : 884
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Book Description
Author: W. Fresenius
Publisher: Springer Science & Business Media
ISBN: 3642697771
Category : Science
Languages : en
Pages : 304
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Book Description
Inorganic chemical pollutants such as S02 and NO. , which also act as the major precursors of acid rain, as well as ecotoxic heavy metals and metalloids (cadmium, lead, mercury, copper, zinc, nickel, chromium, arsenic, selenium etc. ) have gained considerable significance as regards the ecotoxic burden on vegetation, soil and natural waters, and as air pollutants. The rapid increase that has recently been noticed in severe damage to forests has suddenly aroused public interest in the ever-growing problem of inorganic chemical pollutants to a hitherto unknown extent, especially as in addition to the damage to vegetation, this also constitutes a growing hazard for man via food chains. Research on the level, fate and transfer as well as on the ecotoxic and toxic impact of inorganic pollutants has therefore gained considerable significance in science and furthermore as a sound scientific basis for relevant environmental protection regulations, in research politics and in public awareness.
Author: Harriet Nash
Publisher: Springer
ISBN: 0412586207
Category : Science
Languages : en
Pages : 204
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Book Description
Groundwater quality monitoring and testing is of paramount importance both in the developed and developing world. This book presents a series of papers illustrating the varied nature of current research into groundwater quality. Urban and rural supplies are covered through a case history approach, and the importance of remedial action to prevent deterioration is emphasized.
Author: Patrick J. Canney
Publisher:
ISBN:
Category : Amines
Languages : en
Pages : 40
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Book Description
Author: Istvan Bogardi
Publisher: Springer Science & Business Media
ISBN: 3642760406
Category : Technology & Engineering
Languages : en
Pages : 438
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Book Description
The nitrate content of drinking water is rising at an alarming rate in several regions of NATO countries and elsewhere in the world. The increase is due to lack of proper sewage treatment, and primarily to excess fertilizer application. Also, eutrophication in several coastal areas is triggered by high nitrate concentrations. The main purpose of this book is to integrate scientific knowledge related to exposure assessment, health consequences and control of nitrate contamination in water. The motivation is related to the magnitude, the possible adverse health effects, and the high cost of control ling nitrate contamination. Future research tasks are defined by an interaction among hydro logists, toxicologists and environmental engineers in an integrated framework for nitrate risk management. The target readership of this book is a mix of university colleagues, practitioners from both the private and public sectors and advanced graduate students working with the hydrological, health science or environmental engineering aspects of nitrate contamination. The main conclusions include: 1. For risk assessment purposes, knowledge and sufficiently accurate models are available to predict nitrate load and its fate in water under changes in land use. 2. Once agricultural exposure controls are implemented, the response times in ground water may be so long as to make controls unrealistic. 3. It is still unknown whether agricultural best management practice is a compromise between nitrate risk reduction and agricultural revenue. 4. The current drinking water guidelines of 10 mg/L NOrN need not be changed.
Author:
Publisher:
ISBN:
Category : Water
Languages : en
Pages : 984
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Book Description
Author: Nineta Hrastelj
Publisher: Springer
ISBN: 3030203476
Category : Science
Languages : en
Pages : 140
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Book Description
This book presents worked examples of five analytical procedures. These practical examples address traceability, validation and measurement uncertainty aspects in a systematic and consistent way, and cover applications in the analysis of water, food, as well as ores and minerals. This concept is based on the experiences of the TrainMiCc program, in which more than 9000 laboratory professionals all over Europe have participated.
