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Author: Noor Zaliha Raja Abdul Rahman (Raja.)
Publisher: Nova Science Publishers
ISBN: 9781626186453
Category : Medical
Languages : en
Pages : 0
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Book Description
This book serves as a tool to expand the knowledge of researchers in the discipline of microbiology and biochemistry encompassing the fields of molecular biology, structural biology, and crystallography for the two very important industrial enzymes: proteases and lipases. In addition it covers the fermentation technology for the production of locally isolated microbial lipases and proteases. This book presents work done in our laboratory which extensively reviews two major types of lipases and proteases: thermostable and/or organic-solvent tolerant including a chapter on cold-active and organic solvent tolerant lipase and protease isolated from Antarctica. Detailed descriptions on the isolation methods, protein production, protein characterisation, and molecular cloning are discussed. Further works on structural biology of both proteases and lipases which include docking, molecular modification as well as protein crystallisation are also presented. For protein crystallographers, this book highlights a novel discovery of high temperature crystallisation of lipase.In addition, this book also offers a chapter on the cultivation strategy of lipase and protease in yeast expression systems and its bio-reactor studies. In short, this book is an all-in-one quick source of reference for researchers who want to have a quick grasp of understanding on many aspects of enzyme production, starting from the isolation and expression of the enzyme until crystallisation and structure elucidation of the protein.
Author: Noor Zaliha Raja Abdul Rahman (Raja.)
Publisher: Nova Science Publishers
ISBN: 9781626186453
Category : Medical
Languages : en
Pages : 0
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Book Description
This book serves as a tool to expand the knowledge of researchers in the discipline of microbiology and biochemistry encompassing the fields of molecular biology, structural biology, and crystallography for the two very important industrial enzymes: proteases and lipases. In addition it covers the fermentation technology for the production of locally isolated microbial lipases and proteases. This book presents work done in our laboratory which extensively reviews two major types of lipases and proteases: thermostable and/or organic-solvent tolerant including a chapter on cold-active and organic solvent tolerant lipase and protease isolated from Antarctica. Detailed descriptions on the isolation methods, protein production, protein characterisation, and molecular cloning are discussed. Further works on structural biology of both proteases and lipases which include docking, molecular modification as well as protein crystallisation are also presented. For protein crystallographers, this book highlights a novel discovery of high temperature crystallisation of lipase.In addition, this book also offers a chapter on the cultivation strategy of lipase and protease in yeast expression systems and its bio-reactor studies. In short, this book is an all-in-one quick source of reference for researchers who want to have a quick grasp of understanding on many aspects of enzyme production, starting from the isolation and expression of the enzyme until crystallisation and structure elucidation of the protein.
Author: Abu Bakar Salleh
Publisher: Nova Publishers
ISBN: 9781600210686
Category : Medical
Languages : en
Pages : 174
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Book Description
Enzymatic processing of lipids and oils is becoming an important area of research. Hydrolytic enzymes, such as lipases and proteases are being sought after as the biocatalysts. This book focuses on the search and acquisition, isolation and purification and the characterisation of these enzymes
Author: Günter Müller
Publisher: John Wiley & Sons
ISBN: 3527605703
Category : Science
Languages : en
Pages : 354
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Book Description
Lipases and Phospholipases are key control elements in mammalian metabolism. They share many common features that set them apart from other metabolic enzyme classes, most importantly their association with biological membranes. Their potential as drug targets for the treatment of metabolic diseases is widely recognized, and the first lipase inhibitor drugs have been successfully introduced. Providing drug developers with a firm foundation for lipase-centered drug design, the editors of this volume have assembled experts from different scientific disciplines to create a comprehensive handbook for all pharmaceutical chemists, biochemists and physiologists working with lipases. The authors examine fundamental aspects of lipase function in vitro and in vivo, explaining how this knowledge may be used to develop lipase assays. They also treat the physiological roles of lipases in normal and disordered metabolism, as well as strategies to target lipases for the treatment of diabetes, obesity and related disorders. Additional topics include the application of phospholipases for liposome-based drug delivery and their use as diagnostic tools.
Author: Georgina Sandoval
Publisher: Humana Press
ISBN: 9781493986712
Category : Science
Languages : en
Pages : 437
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Book Description
This volume provides an essential update on fundamental issues, current and new applications, as well as practical protocols to explore the extensive applications of lipases and the potential application of phospholipases. After an overview, the book delves into activity screening and expression, optimization of the biocatalyst production and performances, and applications of lipases, phospholipases, and esterases. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Lipases and Phospholipases: Methods and Protocols, Second Editionserves as an updated reference book for the large scientific community, both seasoned and novice, working with lipases, phospholipases, and related enzymes.
Author: Paul Woolley
Publisher: Cambridge University Press
ISBN: 9780521207997
Category : Science
Languages : en
Pages : 0
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Book Description
This 1994 book provides a fascinating account of the fast-moving field of lipase research. The contributions, written by active research workers, summarise developments in the field and give access to recent literature. It covers both the lipases proper (triglyceride lipases and the phospholipases. It gives a comprehensive picture of the state of knowledge of these enzymes, with a strong bias towards the fields that are attracting the greatest attention: their detailed molecular structure, their mechanism of action, their position in the evolution of enzymes, and their application both in the laboratory and industry. The book will continue to be of interest to those working in universities, in research institutes and in companies specialising in biotechnology. The book will also be a useful reference book for postgraduate students entering this field of research.
Author: Stephen Pandol
Publisher: Morgan & Claypool Publishers
ISBN: 1615041389
Category : Medical
Languages : en
Pages : 66
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Book Description
The secretions of the exocrine pancreas provide for digestion of a meal into components that are then available for processing and absorption by the intestinal epithelium. Without the exocrine pancreas, malabsorption and malnutrition result. This chapter describes the cellular participants responsible for the secretion of digestive enzymes and fluid that in combination provide a pancreatic secretion that accomplishes the digestive functions of the gland. Key cellular participants, the acinar cell and the duct cell, are responsible for digestive enzyme and fluid secretion, respectively, of the exocrine pancreas. This chapter describes the neurohumoral pathways that mediate the pancreatic response to a meal as well as details of the cellular mechanisms that are necessary for the organ responses, including protein synthesis and transport and ion transports, and the regulation of these responses by intracellular signaling systems. Examples of pancreatic diseases resulting from dysfunction in cellular mechanisms provide emphasis of the importance of the normal physiologic mechanisms.
Author: Timir Tripathi
Publisher: Academic Press
ISBN: 0323902650
Category : Science
Languages : en
Pages : 716
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Book Description
Advances in Protein Molecular and Structural Biology Methods offers a complete overview of the latest tools and methods applicable to the study of proteins at the molecular and structural level. The book begins with sections exploring tools to optimize recombinant protein expression and biophysical techniques such as fluorescence spectroscopy, NMR, mass spectrometry, cryo-electron microscopy, and X-ray crystallography. It then moves towards computational approaches, considering structural bioinformatics, molecular dynamics simulations, and deep machine learning technologies. The book also covers methods applied to intrinsically disordered proteins (IDPs)followed by chapters on protein interaction networks, protein function, and protein design and engineering. It provides researchers with an extensive toolkit of methods and techniques to draw from when conducting their own experimental work, taking them from foundational concepts to practical application. - Presents a thorough overview of the latest and emerging methods and technologies for protein study - Explores biophysical techniques, including nuclear magnetic resonance, X-ray crystallography, and cryo-electron microscopy - Includes computational and machine learning methods - Features a section dedicated to tools and techniques specific to studying intrinsically disordered proteins
Author: National Institutes of Health (U.S.). Division of Research Grants
Publisher:
ISBN:
Category : Medicine
Languages : en
Pages : 1238
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Book Description
Author: Julio Polaina
Publisher: Springer Science & Business Media
ISBN: 1402053770
Category : Science
Languages : en
Pages : 629
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Book Description
Recent developments in genetic engineering and protein chemistry are bringing ever more powerful means of analysis to bear on the study of enzyme structure. This volume reviews the most important types of industrial enzymes. In a balanced manner it covers three interrelated aspects of paramount importance for enzyme performance: three-dimensional protein structure, physicochemical and catalytic properties, and the range of both classical and novel applications.
Author: Pabulo H. Rampelotto
Publisher:
ISBN: 9783038421474
Category : Biotechnology
Languages : en
Pages : 449
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Book Description
