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Author: S.H. Parvez
Publisher: Elsevier
ISBN: 1483290220
Category : Science
Languages : en
Pages : 436
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Book Description
This two-part book provides the most advanced biochemical and histochemical analytical methods on neurotransmitters, neuromodulators, and neuropeptides described by international specialists. The biochemical methods include liquid chromatography/electrochemistry, liquid chromatography/fluorescence and chemiluminescence detection, gas chromatography or liquid chromatography with mass spectrometry, and immunoassays. Morphological and physiological methods are also described, including immunohistochemistry, in situ hybridization, in vivo microdialysis, electrophysiological analysis, microwave irradiation, electrophysiological analysis, micro-anatomical dissection, peptide sequence analysis, quantitative autoradiographic analysis, and isolated CNS preparations. The neurotransmitters and neuropeptides include catecholamines, indoleamines, histamine, polyamines, and various neuropeptides, and their related enzymes. The book is the most comprehensive, advanced and versatile volume on the biochemical, morphological and physiological analysis of neurotransmitters and neuropeptides.
Author: S.H. Parvez
Publisher: Elsevier
ISBN: 1483290220
Category : Science
Languages : en
Pages : 436
Get Book Here
Book Description
This two-part book provides the most advanced biochemical and histochemical analytical methods on neurotransmitters, neuromodulators, and neuropeptides described by international specialists. The biochemical methods include liquid chromatography/electrochemistry, liquid chromatography/fluorescence and chemiluminescence detection, gas chromatography or liquid chromatography with mass spectrometry, and immunoassays. Morphological and physiological methods are also described, including immunohistochemistry, in situ hybridization, in vivo microdialysis, electrophysiological analysis, microwave irradiation, electrophysiological analysis, micro-anatomical dissection, peptide sequence analysis, quantitative autoradiographic analysis, and isolated CNS preparations. The neurotransmitters and neuropeptides include catecholamines, indoleamines, histamine, polyamines, and various neuropeptides, and their related enzymes. The book is the most comprehensive, advanced and versatile volume on the biochemical, morphological and physiological analysis of neurotransmitters and neuropeptides.
Author: T. Nagatsu
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Book Description
Author: Adalberto Merighi
Publisher: Humana Press
ISBN: 9781617793110
Category : Medical
Languages : en
Pages : 416
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Book Description
Since the early days, the field of neuropeptide biology has dramatically widened, and today the ultimate frontiers in neuropeptide research lie in the development of pharmacologically active compounds that are capable of crossing the blood-brain barrier to exert their biological role(s) in vivo and in the construction of genetic vectors to be employed in gene therapy. Neuropeptides: Methods and Protocols presents a readily reproducible collection of established and emerging techniques for neuropeptide research as contributed by expert researchers in the field. The detailed methods presented cover subjects such as immunocytochemical localization, biochemical characterization, functional analysis, development and production of genetic probes, and the design of neuropeptide derivatives for cellular neurobiology as well as the potential therapeutic applications. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and easy to follow, Neuropeptides: Methods and Protocols is intended for a large audience of scientists, including histologists, biochemists, cellular and molecular biologists, and electrophysiologists that are currently active in the field or are willing to enter this exciting and still expanding area of neurobiology.
Author: Fleur L. Strand
Publisher: MIT Press
ISBN: 9780262194075
Category : Neuropeptides
Languages : en
Pages : 678
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Book Description
This textbook is the first to bring together and synthesize the neuropeptide research of the past decade in such a comprehensive, scholarly manner. In recent years there has been increasing interest and, subsequently, active research in neuropeptides. These neuroactive molecules coordinate, integrate, and regulate physiological processes in all organisms, throughout all phases of development. Acting as neurohormones, neurotransmitters, and/or neuromodulators, they maintain physiological homeostasis and influence important behavioral patterns. This textbook is the first to bring together and synthesize the neuropeptide research of the past decade in such a comprehensive, scholarly manner. The book is divided into two parts. In Part I the author defines the basic principles of neuropeptide action, including their biosynthesis, processing, transport, distribution, and interactions with receptors and second messenger systems. Strand also discusses the intimate interaction between the neuropeptides, stress, and the immune system. In Part II she discusses the regulatory functions of the families of neuropeptide in sufficient detail to provide both the advanced student and senior investigator with a thorough understanding of the most important neuropeptides. The text also contains a complete and up-to-date reference/reading list.
Author: H. Parvez
Publisher:
ISBN:
Category : Neuropeptides
Languages : en
Pages : 488
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Book Description
Author: Bernice A. Levine
Publisher: Nova Publishers
ISBN: 9781600216404
Category : Medical
Languages : en
Pages : 316
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Book Description
The explosion of research activity in the field of neuropeptides has led to the idenfication of numerous naturally occurring endogenous peptides which act as neurotransmitters, neuromodulators, or trophic factors, to mediate nervous system functions. Increasing numbers of non-peptide ligands of neuropeptide receptors have been developed, which act as agonists or antagonists in peptidergic systems. The scope of this book includes gene regulation of peptide expression, peptide receptor subtypes, transgenic and knockout mice with mutations in genes for neuropeptides and peptide receptors, neuroanatomy, physiology, behaviour, neurotrophic factors, preclinical drug evaluation, clinical studies, and clinical trials.
Author: Rafael Covenãs Rodríguez
Publisher:
ISBN: 9788178952918
Category : Neuropeptides
Languages : en
Pages : 362
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Book Description
Neuropeptides is one of the disciplines in the field of Neuroscience that has been largely developed from the seventies and one that is foreseen to develop to possibly unimaginable heights in the near future, since neuropeptides are widespread distributed in the central and peripheral nervous systems and they are involved in many pathologies (Parkinson s disease, Alzheimer s disease, schizophrenia, depression&). Growing numbers of researches (cell biologists, biochemists, geneticists, histologists, pharmacologists, physiologists, psychiatrists, pathologists, immunologists, neurobiologists, psychologists&) are currently involved in this discipline and increasingly sophisticated methods for its study are becoming available. Our main aims in writing this book on neuropeptides were the following: a) to review the general data that exist on neuropeptides; b) to show the results found in several research works on the distribution and functions of neuropeptides; and c) to show the future perspectives on neuropeptide research. In this sense, the text has been divided in four parts. The first entitled Introduction to Neuropeptides includes the general data on neuropeptides such as: concepts of neuropeptide, neurotransmitter, neuromodulator and neurohormone, families of neuropeptides, phylogeny and ontogeny of neuropeptides, biosynthesis of neuropeptides, release and receptors of neuropeptides, enzymatic degradation of neuropeptides, methodologies used in the study of neuropeptides and distribution of neuropeptides (Chapter 1). The second part called Distribution of Neuropeptides includes the results obtained by different research groups on the distribution of neuropeptides in the cat spinal cord, brainstem, diencephalon and cerebral cortex, as well as on the distribution of neuropeptides in the dog brainstem and diencephalon and in the alpaca brainstem (Chapters 2-7). The third part deals with the Functions of the Neuropeptides and includes a review chapter on such functions (involvement of neuropeptides in pain, analgesia, thermoregulation, glucoregulation, food and water intake, immunoregulation, wakefulness and sleep, learning and memory) (Chapter 8) and a specific chapter on the implication of neuropeptides in cardiovascular control (Chapter 9). The aim of the fourth part was to show the Pathophysiology of the Neuropeptides . Thus, there is a chapter in which the involvement of neuropeptides in several pathologies such as asthma, arthritis, Alzheimer s disease, Parkinson s disease, alcoholism, schizophrenia, depression, stress and suicide is reviewed. In such chapter, the implication of neuropeptides in aging will be also reviewed (Chapter 10). Moreover, in the fourth part three specific chapters will be focused on the involvement of neuropeptides in migraine and Parkinson s and Alzheimer s disease (Chapters 11, 12), as well as on the implications of neuropeptides and NK-1 receptor antagonists in cancer research (Chapter 13). This book is directed not only to researchers interested in the field of neuropeptides, but to Life and Health Sciences Ph.D. students. For this reason, we have included three review chapters on the general concerning of neuropeptides (Chapter 1), the functional implications of neuropeptides (Chapter 8) and on the pathophysiology of the neuropeptides (Chapter 10). We hope that this text help to students to be enthusiasm in the study of neuropeptides and to introduce them in this field, since much remains to be studied on the distribution and functions of neuropeptides. Finally, we thank to Mercedes de León the help received before and during the redaction of this book.
Author: Timothy G. Geary
Publisher: Springer Science & Business Media
ISBN: 1441969020
Category : Medical
Languages : en
Pages : 246
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Book Description
The need to continually discover new agents for the control or treatment of invertebrate pests and pathogens is undeniable. Agriculture, both animal and plant, succeeds only to the extent that arthropod and helminth consumers, vectors and pathogens can be kept at bay. Humans and their companion animals are also plagued by invertebrate parasites. The deployment of chemical agents for these purposes inevitably elicits the selection of resistant populations of the targets of control, necessitating a regular introduction of new kinds of molecules. Experience in other areas of chemotherapy has shown that a thorough understanding of the biology of disease is an essential platform upon which to build a discovery program. Unfortunately, investment of research resources into understanding the basic physiology of invertebrates as a strategy to illuminate new molecular targets for pesticide and parasiticide discovery has been scarce, and the pace of introduction of new molecules for these indications has been slowed as a result. An exciting and so far unexploited area to explore in this regard is invertebrate neuropeptide physiology. This book was assembled to focus attention on this promising field by compiling a comprehensive review of recent research on neuropeptides in arthropods and helminths, with contributions from many of the leading laboratories working on these systems.
Author:
Publisher:
ISBN:
Category :
Languages : en
Pages : 564
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Book Description
Neuropeptides are one of the most diverse classes of signaling molecules whose identities and functions are not yet fully understood. They have been implicated in the regulation of a wide range of physiological processes, including feeding-related and motivated behaviors, and also environmental adaptations. In this work, improved mass spectrometry-based analytical platforms were developed and applied to the crustacean systems to characterize signaling molecules. This dissertation begins with a review of mass spectrometry-based neuropeptide studies from both temporal- and spatial-domains. This review is then followed by several chapters detailing a few research projects related to the crustacean neuropeptidomic characterization and comparative analysis. The neuropeptidome of crayfish, Orconectes rusticus is characterized for the first time using mass spectrometry-based tools. In vivo microdialysis sampling technique offers the capability of direct sampling from extracellular space in a time-resolved manner. It is used to investigate the secreted neuropeptide and neurotransmitter content in Jonah crab, Cancer borealis, in this work. A new quantitation strategy using alternative mass spectrometry data acquisition approach is developed and applied for the first time to quantify neuropeptides. Coupling of this method with microdialysis enables the study of neuropeptide dynamics concurrent with different behaviors. Proof-of-principle experiments validating this approach have been carried out in Jonah crab, Cancer borealis to study feeding- and circadian rhythm-related neuropeptide changes using micoridialysis in a time-resolved manner. This permits a close correlation between behavioral and neurochemical changes, providing potential candidates for future validation of regulatory roles. In addition to providing spatial information, mass spectrometry imaging (MSI) technique enables the characterization of signaling molecules while preserving the temporal resolution. A novel MSI-based platform is developed by interfacing with microdialysis sample collection and is applied to the study of in vivo neuropeptide degradation profiles in an off-line ‘real-time’ fashion. This unique platform provides novel insights into neuropeptide inactivation/elimination process, which is an essential step to understand peptidegic signaling pathway. In addition to neuropeptides, this platform has been further modified to study secreted neurotransmitters, and small molecule metabolites that might interact with neuropeptides in crustacean for the first time. This work not only reports improvements upon neuropeptides identification and quantitation by developing improved analytical strategies, but also provides important evidence for the potential roles of several neuropeptides in regulating food intake and circadian rhythm behaviors. Novel platform that integrates MALDI-MSI with microdialysis in exploring neurochemical changes in dynamic biological events is also presented, which could be potentially applied to many other systems in future research. Collectively this dissertation research develops new analytical methods and improves our fundamental understanding of neuropeptide signaling.
Author: Richard E. Brown
Publisher: Cambridge University Press
ISBN: 9780521426657
Category : Science
Languages : en
Pages : 434
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Book Description
This book is designed as an introductory text in neuroendocrinology; the study of the interaction between the brain and endocrine system and the influence of this on behaviour. The endocrine glands, pituitary gland and hypothalamus and their interactions and hormones are discussed. The action of steroid and thyroid hormone receptors and the regulation of target cell response to hormones is examined. The function of neuropeptides is discussed with respect to the neuroendocrine system and behaviour. The neuroimmune system and lymphokines are described and the interaction between the neuroendocrine and neuroimmune systems discussed. Finally, methods for studying hormonal influences on behaviour are outlined. Each chapter has review and essay questions designed for advanced students and honours or graduate students with a background in neuroscience, respectively.
