Biochemistry and Cell Culture PDF Download
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Author: P. Michael Conn
Publisher:
ISBN: 9781283836968
Category : Cell culture
Languages : en
Pages : 505
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Book Description
Cell biology spans among the widest diversity of methods in the biological sciences. From physical chemistry to microscopy, cells have given up with secrets only when the questions are asked in the right way! This new volume of Methods in Cell Biology covers laboratory methods in cell biology, and includes methods that are among the most important and elucidating in the discipline, such as transfection, cell enrichment and magnetic batch separation. Covers the most important laboratory methods in cell biology Chapters written by experts in their fields.
Author: P. Michael Conn
Publisher:
ISBN: 9781283836968
Category : Cell culture
Languages : en
Pages : 505
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Book Description
Cell biology spans among the widest diversity of methods in the biological sciences. From physical chemistry to microscopy, cells have given up with secrets only when the questions are asked in the right way! This new volume of Methods in Cell Biology covers laboratory methods in cell biology, and includes methods that are among the most important and elucidating in the discipline, such as transfection, cell enrichment and magnetic batch separation. Covers the most important laboratory methods in cell biology Chapters written by experts in their fields.
Author: David W. Galbraith
Publisher: Academic Press
ISBN: 0125641516
Category : Medical
Languages : en
Pages : 605
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Book Description
Methods in Plant Cell Biology provides in two volumes a comprehensive collection of analytical methods essential for researchers and students in the plant sciences. Individual chapters, written by experts in the field, provide an introductory overview, followed by a step-by-step technical description of the methods. Key Features * Written by experts, many of whom have developed the individual methods described * Contains most, if not all, the methods needed for modern research in plant cell biology * Up-to-date and comprehensive * Full references * Allows quick access to relevant journal articles and to the sources of chemicals required for the procedures * Selective concentration on higher plant methods allows for particular emphasis on those problems specific to plants.
Author:
Publisher: Elsevier
ISBN: 0080553508
Category : Science
Languages : en
Pages : 473
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Book Description
Cell separation is at the core of current methods in experimental biology and medicine. Its importance is illustrated by the large number of physical and biochemical principles that have been evaluated for application to cell separation. The development of cell separation methods is driven by the needs of biological and medical research, and the ever-increasing demands for sensitivity, selectivity, yield, timeliness and economy of the process. The interdisciplinary nature of research in this area and the volume of information available in research publications and conferences necessitates a basic description of the fundamental processes involved in magnetic cell separation that may help the user in navigating this wealth of information available online and in scientific publications. This book will appeal to researchers in many areas utilizing this technique, including those working in cell biology, clinical research, inorganic chemistry, biochemistry, chemical engineering, materials science, physics and electrical engineering. - Provides examples of how to calculate the volume magnetic susceptibility, a fundamental quantity for calculating the magnetic force acting on a cell, from various types of magnetic susceptibilities available in literature - Introduces the elements of magnetostatics as they apply to cell magnetization and the magnetization of magnetic micro- and nano- particles used for cell separation - Describes the parameters used to determine cell magnetophoresis
Author:
Publisher: Academic Press
ISBN: 012814145X
Category : Science
Languages : en
Pages : 496
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Book Description
Mitosis and Meiosis, Part A, Volume 144, a new volume in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Unique to this updated volume are chapters on Analyzing the Spindle Assembly Checkpoint in human cell culture, an Analysis of CIN, a Functional analysis of the tubulin code in mitosis, Employing CRISPR/Cas9 genome engineering to dissect the molecular requirements for mitosis, Applying the auxin-inducible degradation (AID) system for rapid protein depletion in mammalian cells, Small Molecule Tools in Mitosis Research, Optogenetic control of mitosis with photocaged chemical, and more. - Contains contributions from experts in the field from across the world - Covers a wide array of topics on both mitosis and meiosis - Includes relevant, analysis based topics
Author: Miguel Berrios
Publisher: Academic Press
ISBN: 0125641559
Category : Medical
Languages : en
Pages : 659
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Book Description
This volume is a comprehensive guide to the methodologies used in the study of structural domains of cell nuclei. The text covers chromatin, the karyoskeleton, the soluble domain, and the nucleolus. It details methods that are used to isolate components from these domains and techniques used to assemble and disassemble nuclear elements. There is also coverage of three-dimensional mapping and localization of nuclear processes. Key Features * Provides a practical laboratory guide for studying cell nuclei * Includes comprehensive and easy-to-follow protocols
Author:
Publisher:
ISBN: 9780815332183
Category : Cells
Languages : en
Pages : 0
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Book Description
Author:
Publisher: Academic Press
ISBN: 0124202012
Category : Science
Languages : en
Pages : 609
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Book Description
This new volume, number 123, of Methods in Cell Biology looks at methods for quantitative imaging in cell biology. It covers both theoretical and practical aspects of using optical fluorescence microscopy and image analysis techniques for quantitative applications. The introductory chapters cover fundamental concepts and techniques important for obtaining accurate and precise quantitative data from imaging systems. These chapters address how choice of microscope, fluorophores, and digital detector impact the quality of quantitative data, and include step-by-step protocols for capturing and analyzing quantitative images. Common quantitative applications, including co-localization, ratiometric imaging, and counting molecules, are covered in detail. Practical chapters cover topics critical to getting the most out of your imaging system, from microscope maintenance to creating standardized samples for measuring resolution. Later chapters cover recent advances in quantitative imaging techniques, including super-resolution and light sheet microscopy. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come. Covers sections on model systems and functional studies, imaging-based approaches and emerging studies Chapters are written by experts in the field Cutting-edge material
Author: Martin J. Stoddart
Publisher: Humana Press
ISBN: 9781617791079
Category : Science
Languages : en
Pages : 0
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Book Description
Whether the question is one of basic cell survival, or whether it is being used to correlate cell number to some other factor such as matrix synthesis, an estimate of cell viability is universally required. In Mammalian Cell Viability: Methods and Protocols, experts in the field describe methods from the most basic which can be performed in any laboratory, to some which require specific pieces of equipment. Initially focusing on methods for monolayer and suspension cells, later chapters describe methods for determining viability within tissue sections and 3 dimensional culture systems. Finally, methods requiring highly specialized equipment are described in order to explain what is possible. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital tips on troubleshooting and avoiding known pitfalls. Practical and adaptable, Mammalian Cell Viability: Methods and Protocols serves as a self-contained laboratory manual useful to both experienced researchers and those new to this incredibly important and influential field.
Author: Alasdair M. Gilfillan
Publisher: Springer Science & Business Media
ISBN: 1441995331
Category : Medical
Languages : en
Pages : 312
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Book Description
The editors of Mast Cell Biology, Drs. Gilfillan and Metcalfe, have enlisted an outstanding group of investigators to discuss the emerging concepts in mast cell biology with respect to development of these cells, their homeostasis, their activation, as well as their roles in maintaining health on the one hand and on the other, their participation in disease.
Author: E. N. Willmer
Publisher: Elsevier
ISBN: 1483277232
Category : Science
Languages : en
Pages : 843
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Book Description
Cells and Tissues in Culture: Methods, Biology, and Physiology, Volume 3 focuses on the applications of the methods of tissue culture to various fields of investigation, including virology, immunology, and preventive medicine. The selection first offers information on molecular organization of cells and tissues in culture and tissue culture in radiobiology. Topics include cellular organization at the molecular level, fibrogenesis in tissue culture, effect of radiation on the growth of isolated cells, and irradiation of the selected parts of the cell. The publication then considers the effects of invading organisms on cells and tissues in culture and cell, tissue, and organ cultures in virus research. The book elaborates on antibody production in tissue culture and tissue culture in pharmacology. Discussions focus on early attempts at in vitro studies, tissue culture in the study of pharmacologically active agents, and methods of assessment of drug activity. The text also reviews invertebrate tissue and organ culture in cell research; introduction and methods employed in plant tissue culture; and growth, differentiation and organogenesis in plant tissue and organ cultures. The selection is a vital source of data for readers interested in the culture of cells and tissues.
