Metabolic Engineering of Clostridium Acetobutylicum and Product Extension PDF Download
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Author: Bunmi Busola Omorotionmwan
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Book Description
Author: Bunmi Busola Omorotionmwan
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Book Description
Author: Sang Yup Lee
Publisher: CRC Press
ISBN: 1000105555
Category : Science
Languages : en
Pages : 450
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Book Description
This unique reference/text presents the basic theory and practical applications of metabolic engineering (ME). It offers systematic analysis of complex metabolic pathways and ways of employing recombinant DNA techniques to alter cell behavior, metabolic patterns, and product formation. Treating ME as a distinct subfield of genetic engineering, the book demonstrates new means of enabling cells to produce valuable proteins, polypeptides, and primary and secondary metabolites. Written by more than 35 leading international experts in the field, this book discusses metabolic engineering in plant and mammalian cells, bacteria, and yeasts and assesses metabolic engineering applications in agriculture, pharmaceuticals, and environmental systems. It illuminates the potential of the "cell factory" model for production of chemicals and therapeutics and examines methods for developing new antiviral and antibacterial molecules and effective gene and somatic-cell therapies. Metabolic Engineering also addresses the use of metabolic flux analysis, metabolic control analysis, and online metabolic flux analysis.
Author: Ngoc phuong thao Nguyen
Publisher:
ISBN:
Category :
Languages : en
Pages : 215
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Book Description
Current ly, there is a resurgence of interest in Clostridium acetobutylicum, the biocatalyst of the historical Weizmann process, to produce n-butanol for use both as a bulk chemical and as a renewablc alternative transportation fuel. This thesis describes a method of homologous recombination by replicative plasmid to delete or introduce genes in C. acetobutylicum . This method was successfull y used to delete genes, includin g CACJ502, CAC3535, CAC2879 (upp), to generate C. acetobutylicum. These strains are readily transformable without any previous plasmid methylation and can serve as hosts for a "marker-less" genetic exchange system. A mutant C. acetobutylicum (C. acetobuty licum CAB 1060) was successfully genera ted. This final mutant produces mainly bu tanol, with ethanol and traces of acetate at a molar rati o of 7:1 :1 . This CAB 1060 strain was subjected to a new continuous fermentation process using i) in situ extraction of alcohols by distillation under low pressure and ii) high cell density cultures to increase the titer, yield and productivity of n-butanol production to levels that have never been previously açhieved in any organism . A second homologous recombination method using non-replicative plasmid for marker less gene modification is also described in this thesis. This method allows the simultaneou s inactivation of two genes. lt has been successfully used to construct a mutant unable to produce hydrogen and useful, as a platform strain, for further engineering of C. acetobutylicum to continuously produce bulk chemicals and fuels.
Author: Jens Nielsen
Publisher: Springer Science & Business Media
ISBN: 9783540418481
Category : Bacteria
Languages : en
Pages : 504
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Book Description
Metabolic engineering is a rapidly evolving field that is being applied for the optimization of many different industrial processes. In this issue of Advances in Biochemical Engineering/Biotechnology, developments in different areas of metabolic engineering are reviewed. The contributions discuss the application of metabolic engineering in the improvement of yield and productivity - illustrated by amino acid production and the production of novel compounds - in the production of polyketides and extension of the substrate range - and in the engineering of S. cerevisiae for xylose metabolism, and the improvement of a complex biotransformation process.
Author: Cynthia Kelechi Akaluka
Publisher:
ISBN:
Category :
Languages : en
Pages : 0
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Author: Teng Bao
Publisher:
ISBN:
Category : Biochemical engineering
Languages : en
Pages : 205
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Book Description
Clostridium cellulovorans is a strictly anaerobic, cellulolytic bacterium among the most interesting candidates for CBP of lignocellulosic biomass. Recently, a bifunctional aldehyde/alcohol dehydrogenase gene adhE2 from Clostridium acetobutylicum has been overexpressed in C. cellulovorans for n-butanol production from cellulose. However, this recombinant strain produced n-butanol from microcrystalline cellulose at a low titer and yield insufficient for industrial application. In addition, there are two type II restriction modification (RM) systems in C. cellulovorans and currently available plasmids would be digested by C. cellulovorans cell extract even after in vivo methylation, making it difficult to transfer and express genes in C. cellulovorans for further metabolic engineering. Therefore, efficient transformation for developing better recombinant C. cellulovorans for butanol production is urgently required.
Author: Sang Yup Lee
Publisher: CRC Press
ISBN: 1000148769
Category : Science
Languages : en
Pages : 453
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Book Description
This unique reference/text presents the basic theory and practical applications of metabolic engineering (ME). It offers systematic analysis of complex metabolic pathways and ways of employing recombinant DNA techniques to alter cell behavior, metabolic patterns, and product formation. Treating ME as a distinct subfield of genetic engineering, the book demonstrates new means of enabling cells to produce valuable proteins, polypeptides, and primary and secondary metabolites. Written by more than 35 leading international experts in the field, this book discusses metabolic engineering in plant and mammalian cells, bacteria, and yeasts and assesses metabolic engineering applications in agriculture, pharmaceuticals, and environmental systems. It illuminates the potential of the "cell factory" model for production of chemicals and therapeutics and examines methods for developing new antiviral and antibacterial molecules and effective gene and somatic-cell therapies. Metabolic Engineering also addresses the use of metabolic flux analysis, metabolic control analysis, and online metabolic flux analysis.
Author: H. W. Doelle
Publisher: Academic Press
ISBN: 1483272745
Category : Nature
Languages : en
Pages : 501
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Book Description
Bacterial Metabolism focuses on metabolic events that occur in microorganisms, as well as photosynthesis, oxidation, polysaccharide formation, and homofermentation. The book first discusses the thermodynamics of biological reactions, photosynthesis and photometabolism, and chemosynthesis. Free energy, photosynthesis, enzymes, and terminology in bacterial metabolism are elaborated. The manuscript then examines acetic acid bacteria and lactic acid bacteria. Discussions focus on lactate, ethanol, glucose, and glycerol metabolism, glycol oxidation, homofermentation, polysaccharide formation, and electron transport systems. The publication takes a look at pseudomonadaceae and nitrogen metabolism as an energy source for anaerobic microorganisms. Topics include metabolism of pairs of amino acids, single amino acid metabolism, oxidation of glycolate and malonate, and oxygenases. The book is a dependable source of information for readers interested in bacterial metabolism.
Author: Ruchir Pradip Desai
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Book Description
Next, the improved model was used to analyze fermentation data from several strains of C. acetobutylicum ATCC 824. Metabolic flux analysis revealed previously obscured roles for the acid formation pathways. The acetate formation pathway supported significant fluxes throughout the stationary phase while the butyrate formation pathway was found to uptake butyrate. Metabolic flux analysis of a butyrate kinase knockout strain revealed that the enhanced solvent production characteristic was due to early induction of butanol formation as opposed to substantially higher butanol formation fluxes. Analysis also revealed enhanced uptake of acetate and substantially reduced butyrate formation and uptake.
Author: George Stephanopoulos
Publisher: Elsevier
ISBN: 008053628X
Category : Science
Languages : en
Pages : 749
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Book Description
Metabolic engineering is a new field with applications in the production of chemicals, fuels, materials, pharmaceuticals, and medicine at the genetic level. The field's novelty is in the synthesis of molecular biology techniques and the tools of mathematical analysis, which allow rational selection of targets for genetic modification through measurements and control of metabolic fluxes. The objective is to identify specific genetics or environmental manipulations that result in improvements in yield and productivities of biotechnological processes. Key features of the book are pathway integration and the focus on metabolic flux as a fundamental determinant of cell physiology. The book keeps mathematical complexity to a minimum, and provides a glossary of biological terms to facilitate use of the book by a broader spectrum of readers. A web page exists to communicate updates of the codes and homework problems. - Demonstrates metabolic engineering in action with numerous examples of pathway modification - Includes methods for identifying key enzymes in metabolic networks - Contains a comprehensive review of metabolic biochemistry - Discusses metabolic regulation at the gene, enzyme, operon, and cell levels - Explains concepts of stoichiometry, kinetics, and thermodynamics of metabolic pathways - Minimizes mathematical complexity - Links to a Web page to communicate updates of the software code and homework problems
