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Author:
Publisher: Academic Press
ISBN: 0128030747
Category : Science
Languages : en
Pages : 513
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Book Description
Isotope Labeling of Biomolecules: Applications, the latest in the Methods in Enzymology series, focuses on stable isotope labeling methods and applications for biomolecules. This practical guide to biomolecular labeling looks at new techniques that are becoming widely used. - Continues the legacy of this premier serial with quality chapters authored by leaders in the field - Focuses on stable isotope labeling of biomolecules, which is important for structural studies of proteins and nucleic acids
Author:
Publisher: Academic Press
ISBN: 0128030747
Category : Science
Languages : en
Pages : 513
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Book Description
Isotope Labeling of Biomolecules: Applications, the latest in the Methods in Enzymology series, focuses on stable isotope labeling methods and applications for biomolecules. This practical guide to biomolecular labeling looks at new techniques that are becoming widely used. - Continues the legacy of this premier serial with quality chapters authored by leaders in the field - Focuses on stable isotope labeling of biomolecules, which is important for structural studies of proteins and nucleic acids
Author:
Publisher: Academic Press
ISBN: 0128030801
Category : Science
Languages : en
Pages : 662
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Book Description
Isotope Labeling of Biomolecules – Labeling Methods, the latest volume of the Methods in Enzymology series contains comprehensive information on stable isotope labeling methods and applications for biomolecules. - Contains contributions from leading authorities in the field of isotope labeling of biomolecules - Informs and updates on the latest developments in the field - Provides comprehensive information on stable isotope labeling methods and applications for biomolecules
Author: Jill Trewhella
Publisher:
ISBN:
Category : Biomolecules
Languages : en
Pages : 392
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Book Description
Author: Greg T. Hermanson
Publisher: Academic Press
ISBN: 0080568726
Category : Science
Languages : en
Pages : 1233
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Book Description
Bioconjugate Techniques, 2nd Edition, is the essential guide to the modification and cross linking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions with details on hundreds of commercially available reagents and the use of these reagents for modifying or cross linking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers. A one-stop source for proven methods and protocols for synthesizing bioconjugates in the lab Step-by-step presentation makes the book an ideal source for researchers who are less familiar with the synthesis of bioconjugates More than 600 figures that visually describe the complex reactions associated with the synthesis of bioconjugates Includes entirely new chapters on the latest areas in the field of bioconjugation as follows: Microparticles and nanoparticlesSilane coupling agentsDendrimers and dendronsChemoselective ligationQuantum dotsLanthanide chelatesCyanine dyesDiscrete PEG compoundsBuckyballs,fullerenes, and carbon nanotubesMass tags and isotope tagsBioconjugation in the study of protein interactions
Author: Xianlin Han
Publisher: John Wiley & Sons
ISBN: 1118893123
Category : Science
Languages : en
Pages : 48
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Book Description
Covers the area of lipidomics from fundamentals and theory to applications Presents a balanced discussion of the fundamentals, theory, experimental methods and applications of lipidomics Covers different characterizations of lipids including Glycerophospholipids; Sphingolipids; Glycerolipids and Glycolipids; and Fatty Acids and Modified Fatty Acids Includes a section on quantification of Lipids in Lipidomics such as sample preparation; factors affecting accurate quantification; and data processing and interpretation Details applications of Lipidomics Tools including for Health and Disease; Plant Lipidomics; and Lipidomics on Cellular Membranes
Author: Ulrich Pleiss
Publisher:
ISBN:
Category : Science
Languages : en
Pages : 736
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Book Description
This outstanding book covers the most recent advances in the production of stable and radioactive isotopes as well as in the synthesis, analysis and applications of isotopically labelled compounds. Written by internationally renowned contributors, it provides authoritative and wide-ranging coverage of significant growth areas including: * new methodologies in the production of isotopes * novel approaches to compounds labelled with isotopes of hydrogen, carbon and other stable, long-lived and short-lived radioactive isotopes * advances in the analysis of istopically labelled compounds * recent and future applications of isotopes in pharmaceutical research and development, in pharmacological and clinical sutdies and nuclear medicine, as well as in agricultural, nutritional and environmental research * recent applications of isotopes in investigating organic and bioorganic mechanisms and pathways * safety and regulations of handling isotopes This will be an invaluable information and reference source to anyone involved in the production of isotopes, the synthesis and analysis of compounds labelled with stable, long-lived or short-lived radioactive isotopes, in drug research and development, in clinical investigations and nuclear medicine, in agricultural research and development and environmental investigations, in the elucidation of biological and chemical pathways, handling radioisotopes, laboratory design and other areas.
Author: Ji-Xin Cheng
Publisher: Elsevier
ISBN: 0323903371
Category : Science
Languages : en
Pages : 612
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Book Description
Stimulated Raman Scattering Microscopy: Techniques and Applications describes innovations in instrumentation, data science, chemical probe development, and various applications enabled by a state-of-the-art stimulated Raman scattering (SRS) microscope. Beginning by introducing the history of SRS, this book is composed of seven parts in depth including instrumentation strategies that have pushed the physical limits of SRS microscopy, vibrational probes (which increased the SRS imaging functionality), data science methods, and recent efforts in miniaturization. This rapidly growing field needs a comprehensive resource that brings together the current knowledge on the topic, and this book does just that. Researchers who need to know the requirements for all aspects of the instrumentation as well as the requirements of different imaging applications (such as different types of biological tissue) will benefit enormously from the examples of successful demonstrations of SRS imaging in the book. Led by Editor-in-Chief Ji-Xin Cheng, a pioneer in coherent Raman scattering microscopy, the editorial team has brought together various experts on each aspect of SRS imaging from around the world to provide an authoritative guide to this increasingly important imaging technique. This book is a comprehensive reference for researchers, faculty, postdoctoral researchers, and engineers. - Includes every aspect from theoretic reviews of SRS spectroscopy to innovations in instrumentation and current applications of SRS microscopy - Provides copious visual elements that illustrate key information, such as SRS images of various biological samples and instrument diagrams and schematics - Edited by leading experts of SRS microscopy, with each chapter written by experts in their given topics
Author: J. R. Heys
Publisher:
ISBN:
Category : Reference
Languages : en
Pages : 776
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Book Description
Synthesis and Applications of Isotopically Labelled Compounds provides a wide-ranging, authoritative and comprehensive coverage of the most recent advances in the synthesis, production and applications of stable and radioactive isotopes. This volume focuses on significant growth areas including applications of isotopes in clinical research and medicine, advances in the analysis of isotopically labelled compounds, novel approaches to compounds labelled with isotopes of hydrogen, applications of isotopes in organic and bio-organic reaction mechanisms, isotopes in macro-molecules, applications of isotopes in metabolism and toxicology, production of stable isotopes and radioisotopes and applications of isotopes in pharmaceutical and agricultural research. Contributions are drawn from major research groups around the world providing an invaluable information and reference source to anyone involved in the production of isotopes, the synthesis and analysis of stable or radioactive isotopically labelled compounds, the applications of short-lived isotopes, drug metabolism and pharmacokinetics, pharmaceutical research and development, clinical research, biosynthesis, handling radioisotopes, laboratory design and other related areas.
Author: Ting-Jia Gu
Publisher:
ISBN:
Category :
Languages : en
Pages : 0
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Book Description
Biomolecules are essential for the survival and growth of all living organisms, serving as building blocks for various cell structures and performing various physiological functions. Four major types of biomolecules exist in organisms, including carbohydrates, proteins, lipids, and nucleic acids, each performing unique functions in the body and oftentimes working together with other biomolecules to orchestrate complex biological processes. To fully understand the mechanisms and significance of biomolecules, comprehensive characterization and quantification of these molecular players are crucial, requiring the development of sensitive and informative techniques such as mass spectrometry (MS) for analyzing diverse types of biomolecules. To further enhance the information content and overcome technical limitations of MS-based approaches, chemicalderivatization is often employed, with the goal to improve ionization efficiency, enable isomer differentiation, enrichment, and quantitative analysis. Isobaric labeling is an example of chemical derivatization used for multiplexed quantitative analysis. This strategy was initially developed for collision-induced dissociation-based quantitative proteomics, but has recently been extended to multiplexed quantitative analysis of other essential biomolecules, such as carbohydrates and lipids. The development and application of isobaric labeling strategy for multiplexed quantitative analysis is a promising approach to high-throughput bioanalysis of multiple samples, which will help to expand our understanding of biomolecule functions, biological processes, potential pathogenesis, and ultimately lead to the discovery of biomarkers or therapeutic targets. This dissertation focuses on the development and application of isobaric labeling strategy for multiplexed quantitative analysis of several major classes of biomolecules. A portion of this work provides a comprehensive overview of isobaric tagging approaches and their recent advancements, current limitations, and multiple applications, including biomarker identification, structural studies, single-cell examination, and the application to diverse molecules such as neuropeptides, glycans, metabolites, and lipids. Furthermore, this dissertation described several novel isobaric labeling strategies coupled with additional chemical derivatization to expand this technique to quantitative lipidomics, enabling multiplexed quantification of phospholipids and fatty acids with double bonds localized. Besides lipidomics, a new set of DiLeuPMP and DiLeu isobaric mass tags with different isotopic configurations were also designed to achieve isobaric labeling quantitation for O-glycomics and electron-transfer dissociation (ETD)-based proteomics. This dissertation showcases the evolution and implementation of advanced MS techniques and isobaric labeling strategies for quantitative biomolecule analysis, demonstrating their potential applications in pharmaceutical and biomedical research. These developments will further expand the application of isobaric labeling strategies in biomolecule analysis and have great potential in pharmaceutical and clinical research.
Author: Jose Alonso
Publisher: Royal Society of Chemistry
ISBN: 1788018168
Category : Science
Languages : en
Pages : 473
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Book Description
Isotope Dilution Mass Spectrometry (IDMS) has become an essential tool in research laboratories and is increasingly used in routine analysis labs (including environmental, food safety and clinical applications). This is the first textbook to present a comprehensive and instructive view of the theory and applications of this growing technique. The main objective of this book is to cover the theory and applications of Isotope Dilution in Analytical Chemistry. The scope is comprehensive to include elemental analysis, speciation analysis, organic analysis and biochemical and clinical analysis together with applications in metabolism studies and traceability of goods. Until now there have been no books published with the same general scope (only book chapters on particular applications). This is a textbook focused at post-graduate level covering the basic knowledge required for doctoral studies in this field. Isotope Dilution Mass Spectrometry will also outline practical applications of interest for routine testing laboratories where isotope dilution procedures are implemented or can be implemented in the future. This unique book covers all the theoretical and practical aspects of Isotope Dilution Mass Spectrometry (IDMS). Due to the increasing application of IDMS in many research laboratories and the increasing implementation of IDMS methodologies in routine testing laboratories, scientists in industry and working in or affiliated to this area will this an invaluable source of information. Concerning the theoretical aspects, the authors present a uniform theoretical background which grows from previous developments in Organic, Speciation and Elemental analysis both in their own laboratory and in other laboratories around the world. This general approach will be simpler and will also include new emerging fields such as quantitative proteomics and metabolism studies.
