Ion-Exchange Chromatography of Proteins

Ion-Exchange Chromatography of Proteins PDF Author: Shuichi Yamamoto
Publisher: CRC Press
ISBN: 9780824779030
Category : Science
Languages : en
Pages : 438

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Book Description
Showing chemists how to predict ion-exchange chromatography (IEC) separation behavior and how to determine appropriate operating conditions, this reference illustrates procedures, apparatus, and types of ion exchangers, emphasizing the design and application of large-scale IEC. Complete with more than 100 useful tables and diagrams, Ion-Exchange Chromatography of Proteins explains the effects of each variable on separation behavior ... compares models and equations describing separation behavior of proteins in IEC with experimental results ... introduces design calculation procedures for scale up, adapting IEC for individual requirements ... discusses parameters affecting separation behavior, including adsorption equilibria, stationary phase diffusion, and axial dispersion ... provides examples of the most up-to-date applications, such as high- or medium-performance IEC and large-scale operations ... and reviews both theoretical and experimental literature. Ion-Exchange Chromatography of Proteins serves as an important reference for analytical, agricultural, food, and pharmaceutical chemists, chromatographers, food scientists and technologists, biochemists, and biotechnologists. Book jacket.

Ion-Exchange Chromatography of Proteins

Ion-Exchange Chromatography of Proteins PDF Author: Shuichi Yamamoto
Publisher: CRC Press
ISBN: 9780824779030
Category : Science
Languages : en
Pages : 438

Get Book Here

Book Description
Showing chemists how to predict ion-exchange chromatography (IEC) separation behavior and how to determine appropriate operating conditions, this reference illustrates procedures, apparatus, and types of ion exchangers, emphasizing the design and application of large-scale IEC. Complete with more than 100 useful tables and diagrams, Ion-Exchange Chromatography of Proteins explains the effects of each variable on separation behavior ... compares models and equations describing separation behavior of proteins in IEC with experimental results ... introduces design calculation procedures for scale up, adapting IEC for individual requirements ... discusses parameters affecting separation behavior, including adsorption equilibria, stationary phase diffusion, and axial dispersion ... provides examples of the most up-to-date applications, such as high- or medium-performance IEC and large-scale operations ... and reviews both theoretical and experimental literature. Ion-Exchange Chromatography of Proteins serves as an important reference for analytical, agricultural, food, and pharmaceutical chemists, chromatographers, food scientists and technologists, biochemists, and biotechnologists. Book jacket.

Handbook of Ion Chromatography

Handbook of Ion Chromatography PDF Author: Joachim Weiss
Publisher:
ISBN: 9780961717308
Category : Science
Languages : en
Pages : 244

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Book Description


Ion Exchange in Analytical Chemistry

Ion Exchange in Analytical Chemistry PDF Author: William Rieman
Publisher: Elsevier
ISBN: 1483186512
Category : Science
Languages : en
Pages : 312

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Book Description
Analytical Chemistry, Volume 38: Ion Exchange in Analytical Chemistry provides a broad survey of the important role that ion exchange can and should play in chemical analysis. This book focuses on the plate-equilibrium theory of chromatography, which is less difficult theoretically than the mass-transfer theory. Organized into 11 chapters, this volume begins with an overview of the earliest recorded application of ion exchange. This text then examines how high temperature affects ion-exchange resins. Other chapters consider the exchange of ions between a solid ion-exchanging material and a solution, which is a typically reversible reaction. This book describes as well the relatively simple separations and other applications of ion exchange to analytical chemistry. The final chapter deals with the interesting nature of the metal complexes formed within the exchanger and describe the use of ion-exchange distribution studies to determine the stability and nature of complexes existing in the solution. This book is a valuable resource for analytical chemists.

Applications of Ion Exchange Materials in Biomedical Industries

Applications of Ion Exchange Materials in Biomedical Industries PDF Author: Inamuddin
Publisher: Springer
ISBN: 3030060829
Category : Medical
Languages : en
Pages : 225

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Book Description
This book presents the applications of ion-exchange materials in the biomedical industries. It includes topics related to the application of ion exchange chromatography in determination, extraction and separation of various compounds such as amino acids, morphine, antibiotics, nucleotides, penicillin and many more. This title is a highly valuable source of knowledge on ion-exchange materials and their applications suitable for postgraduate students and researchers but also to industrial R&D specialists in chemistry, chemical, and biochemical technology. Additionally, this book will provide an in-depth knowledge of ion-exchange column and operations suitable for engineers and industrialists.

Protein Purification

Protein Purification PDF Author: Jan-Christer Janson
Publisher: John Wiley & Sons
ISBN: 9780471186267
Category : Medical
Languages : en
Pages : 712

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Book Description
This is a state-of-the-art sourcebook on modern high-resolution biochemical separation techniques for proteins. It contains all the basic theory and principles used in protein chromatography and electrophoresis.

Applications of Ion Chromatography for Pharmaceutical and Biological Products

Applications of Ion Chromatography for Pharmaceutical and Biological Products PDF Author: Lokesh Bhattacharyya
Publisher: John Wiley & Sons
ISBN: 1118146999
Category : Medical
Languages : en
Pages : 421

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Book Description
This is a comprehensive source of information on the application of ion chromatography (IC) in the analysis of pharmaceutical drugs and biologicals. This book, with contributors from academia, pharma, the biotech industry, and instrument manufacturing, presents the different perspectives, experience, and expertise of the thought leaders of IC in a comprehensive manner. It explores potential IC applications in different aspects of product development and quality control testing. In addition, an appendix section gives information on critical physical and chromatographic parameters related to IC and information on current manufacturers of IC systems, columns, and other components.

Ion Exchange Chromatography

Ion Exchange Chromatography PDF Author: Pharmacia LKB Biotechnology
Publisher:
ISBN: 9789197049030
Category : Ion exchange chromatography
Languages : en
Pages : 119

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Book Description


Protein Chromatography

Protein Chromatography PDF Author: Dermot Walls
Publisher: Humana Press
ISBN: 9781607619147
Category : Science
Languages : en
Pages : 527

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Book Description
A prerequisite for elucidating the structure and function of any protein is the prior purification of that protein. This necessity has led to the development of many purification schemes and chromatographic methods for the isolation of native proteins from complex sources. In Protein Chromatography: Methods and Protocols, leading researchers present clear protocol-style chapters that are suitable for newcomers and experts alike. The book opens with vital topics in protein biochemistry, addressing such areas as protein stability and storage, avoiding proteolysis during chromatography, protein quantitation methods including immuno-qPCR, and the contrasting challenges that microfluidics and scale-up production pose to the investigator, and then it segues into key methods involving the generation and purification of recombinant proteins through recombinant antibody production and the tagging of proteins, amongst other means, as well as many variations on classic techniques such as ion-exchange and immunoaffinity chromatography. Written in the highly successful Methods in Molecular BiologyTM series format, protocols chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Protein Chromatography: Methods and Protocols will greatly aid scientists in establishing these essential techniques in their own laboratories and furthering our understanding of the many imperative functions of proteins.

Ion Chromatography

Ion Chromatography PDF Author: James S. Fritz
Publisher: John Wiley & Sons
ISBN: 3527613250
Category : Science
Languages : de
Pages : 267

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Book Description
Reflecting the tremendous development of ion chromatography in recent years, the best-selling book by Fritz and Gjerde has now gone into a third edition. This is essentially a new book, describing materials, principles, and methods of ion chromatography in a clear and concise style. The book can be used both as an introduction for the new comer and as a practical guide for method development and applications for the experienced user. It contains handy tables with useful data, e. g. on detection and elution conditions. With this new edition, the scope has been enlarged to include capillary electrophoresis as well as chemical speciation. The readers of this book will profit from the authors' background and experience both in education and industrial application.

Ion Exchange

Ion Exchange PDF Author: F.C. Nachod
Publisher: Elsevier
ISBN: 0323151000
Category : Science
Languages : en
Pages : 424

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Book Description
Ion Exchange: Theory and Application focuses on the applications, complexities, and theoretical aspects of ion exchange. This book discusses the kinetics of fixed-bed ion exchange; fundamental properties of ion exchange resins; ion exchange equipment design; and ion exchange in water treatment. The multistage systems in ion exchange; desalting sea water; applications of ion exchange to the separation of inorganic cations; and ion exchange as a tool in analytical chemistry are also elaborated. This text likewise covers the metal concentration and recovery by ion exchange; catalytic application of ion exchangers; and use of ion exchange adsorbents in biochemical and physiological studies. Other topics include the separation of amino acids by ion exchange chromatography; sugar refining and by-product recovery; and ion exchange recovery of alkaloids. This publication is a good reference for chemists and students interested in ion exchange.