Author: Jim Mcwhir
Publisher: Garland Science
ISBN: 0203489241
Category : Science
Languages : en
Pages : 218
Book Description
Gene Targeting and Embryonic Stem Cells is a practical guide designed for the rapidly growing number of researchers who are moving into this field. Provides details on how to culture, transfect and differentiate established cell lines, and how to isolate new cell lines. Gene targeting experiments are described for a number of cell types, including ungulate fetal fibroblasts, murine ES cells, human embryonal carinoma cells and human ES cells, and include protocols for gene-targeting vectors, DNA transfection and RNA interference.
Gene Targeting and Embryonic Stem Cells
Author: Jim Mcwhir
Publisher: Garland Science
ISBN: 0203489241
Category : Science
Languages : en
Pages : 218
Book Description
Gene Targeting and Embryonic Stem Cells is a practical guide designed for the rapidly growing number of researchers who are moving into this field. Provides details on how to culture, transfect and differentiate established cell lines, and how to isolate new cell lines. Gene targeting experiments are described for a number of cell types, including ungulate fetal fibroblasts, murine ES cells, human embryonal carinoma cells and human ES cells, and include protocols for gene-targeting vectors, DNA transfection and RNA interference.
Publisher: Garland Science
ISBN: 0203489241
Category : Science
Languages : en
Pages : 218
Book Description
Gene Targeting and Embryonic Stem Cells is a practical guide designed for the rapidly growing number of researchers who are moving into this field. Provides details on how to culture, transfect and differentiate established cell lines, and how to isolate new cell lines. Gene targeting experiments are described for a number of cell types, including ungulate fetal fibroblasts, murine ES cells, human embryonal carinoma cells and human ES cells, and include protocols for gene-targeting vectors, DNA transfection and RNA interference.
Gene Targeting in Mouse Embryonic Stem Cells
Author: Laura Hink Reid
Publisher:
ISBN:
Category :
Languages : en
Pages : 388
Book Description
Publisher:
ISBN:
Category :
Languages : en
Pages : 388
Book Description
Gene Knockout Protocols
Author: Martin J. Tymms
Publisher: Springer Science & Business Media
ISBN: 1592592201
Category : Medical
Languages : en
Pages : 434
Book Description
As the major task of sequencing the human genome is near completion and full complement of human genes are catalogued, attention will be focused on the ultimate goal: to understand the normal biological functions of these genes, and how alterations lead to disease states. In this task there is a severe limitation in working with human material, but the mouse has been adopted as the favored animal model because of the available genetic resources and the highly conserved gene conservation linkage organization. In just of ten years since the first gene-targeting experiments were p- formed in embryonic stem (ES) cells and mutations transmitted through the mouse germline, more than a thousand mouse strains have been created. These achievements have been made possible by pioneering work that showed that ES cells derived from preimplantation mouse embryos could be cultured for prolonged periods without differentiation in culture, and that homologous rec- bination between targeting constructs and endogenous DNA occurred at a f- quency sufficient for recombinants to be isolated. In the next few years the mouse genome will be systematically altered, and the techniques for achi- ing manipulations are constantly being streamlined and improved.
Publisher: Springer Science & Business Media
ISBN: 1592592201
Category : Medical
Languages : en
Pages : 434
Book Description
As the major task of sequencing the human genome is near completion and full complement of human genes are catalogued, attention will be focused on the ultimate goal: to understand the normal biological functions of these genes, and how alterations lead to disease states. In this task there is a severe limitation in working with human material, but the mouse has been adopted as the favored animal model because of the available genetic resources and the highly conserved gene conservation linkage organization. In just of ten years since the first gene-targeting experiments were p- formed in embryonic stem (ES) cells and mutations transmitted through the mouse germline, more than a thousand mouse strains have been created. These achievements have been made possible by pioneering work that showed that ES cells derived from preimplantation mouse embryos could be cultured for prolonged periods without differentiation in culture, and that homologous rec- bination between targeting constructs and endogenous DNA occurred at a f- quency sufficient for recombinants to be isolated. In the next few years the mouse genome will be systematically altered, and the techniques for achi- ing manipulations are constantly being streamlined and improved.
Gene Targeting by Single-stranded DNA Oligonucleotides in Mouse Embryonic Stem Cells
Author: Marieke Aarts
Publisher:
ISBN: 9789461080158
Category :
Languages : en
Pages : 155
Book Description
Publisher:
ISBN: 9789461080158
Category :
Languages : en
Pages : 155
Book Description
Gene Targeting
Author: Alexandra Joyner
Publisher: OUP Oxford
ISBN: 0191566020
Category : Science
Languages : en
Pages : 312
Book Description
Since the publication of the first edition of Gene Targeting: A Practical Approach in 1993 there have been many advances in gene targeting and this new edition has been thoroughly updated and rewritten to include all the major new techniques. It provides not only tried-and-tested practical protocols but detailed guidance on their use and applications. As with the previous edition Gene Targeting: A Practical Approach 2e concentrates on gene targeting in mouse ES cells, but the techniques described can be easily adapted to applications in tissue culture including those for human cells. The first chapter covers the design of gene targeting vectors for mammalian cells and describes how to distinguish random integrations from homologous recombination. It is followed by a chapter on extending conventional gene targeting manipulations by using site-specific recombination using the Cre-loxP and Flp-FRT systems to produce 'clean' germline mutations and conditionally (in)activating genes. Chapter 3 describes methods for introducing DNA into ES cells for homologous recombination, selection and screening procedures for identifying and recovering targeted cell clones, and a simple method for establishing new ES cell lines. Chapter 4 discusses the pros and cons or aggregation versus blastocyst injection to create chimeras, focusing on the technical aspects of generating aggregation chimeras and then describes some of the uses of chimeras. The next topic covered is gene trap strategies; the structure, components, design, and modification of GT vectors, the various types of GT screens, and the molecular analysis of GT integrations. The final chapter explains the use of classical genetics in gene targeting and phenotype interpretation to create mutations and elucidate gene functions. Gene Targeting: A Practical Approach 2e will therefore be of great value to all researchers studying gene function.
Publisher: OUP Oxford
ISBN: 0191566020
Category : Science
Languages : en
Pages : 312
Book Description
Since the publication of the first edition of Gene Targeting: A Practical Approach in 1993 there have been many advances in gene targeting and this new edition has been thoroughly updated and rewritten to include all the major new techniques. It provides not only tried-and-tested practical protocols but detailed guidance on their use and applications. As with the previous edition Gene Targeting: A Practical Approach 2e concentrates on gene targeting in mouse ES cells, but the techniques described can be easily adapted to applications in tissue culture including those for human cells. The first chapter covers the design of gene targeting vectors for mammalian cells and describes how to distinguish random integrations from homologous recombination. It is followed by a chapter on extending conventional gene targeting manipulations by using site-specific recombination using the Cre-loxP and Flp-FRT systems to produce 'clean' germline mutations and conditionally (in)activating genes. Chapter 3 describes methods for introducing DNA into ES cells for homologous recombination, selection and screening procedures for identifying and recovering targeted cell clones, and a simple method for establishing new ES cell lines. Chapter 4 discusses the pros and cons or aggregation versus blastocyst injection to create chimeras, focusing on the technical aspects of generating aggregation chimeras and then describes some of the uses of chimeras. The next topic covered is gene trap strategies; the structure, components, design, and modification of GT vectors, the various types of GT screens, and the molecular analysis of GT integrations. The final chapter explains the use of classical genetics in gene targeting and phenotype interpretation to create mutations and elucidate gene functions. Gene Targeting: A Practical Approach 2e will therefore be of great value to all researchers studying gene function.
Creating Targeted Mutations in Mouse Embryonic Stem Cells Using Yeast Artificial Chromosomes
Author: Rebecca Michelle Tucker
Publisher:
ISBN:
Category :
Languages : en
Pages : 266
Book Description
Publisher:
ISBN:
Category :
Languages : en
Pages : 266
Book Description
Gene Targeting in Murine Embryonic Stem Cells
Author: Chuxia Deng
Publisher:
ISBN:
Category : Genetic engineering
Languages : en
Pages : 434
Book Description
Publisher:
ISBN:
Category : Genetic engineering
Languages : en
Pages : 434
Book Description
Transgenic Animal Technology
Author: Carl A. Pinkert
Publisher: Newnes
ISBN: 0124095364
Category : Science
Languages : en
Pages : 715
Book Description
Transgenic animal technologies and the ability to introduce functional genes into animals have revolutionized our ability to address complex biomedical and biological questions. This well-illustrated handbook covers the technical aspects of gene transfer — from molecular methods to whole animal considerations — for important laboratory and domestic animal species. It describes methodologies as employed by leading laboratories and is a key resource for researchers, as well as a tool for training technicians and students. This second edition incorporates updates on a variety of genetic engineering technologies ranging from microinjection and ES cell transfer to nuclear transfer in a broad range of animal modeling systems. Contains a comprehensive collection of transgenic animal and gene transfer methods Discusses background and introduction to techniques and animal systems Teaches practical step-by-step protocols Fully revised with updates to reflect state-of-the-art technology and associated changes to date
Publisher: Newnes
ISBN: 0124095364
Category : Science
Languages : en
Pages : 715
Book Description
Transgenic animal technologies and the ability to introduce functional genes into animals have revolutionized our ability to address complex biomedical and biological questions. This well-illustrated handbook covers the technical aspects of gene transfer — from molecular methods to whole animal considerations — for important laboratory and domestic animal species. It describes methodologies as employed by leading laboratories and is a key resource for researchers, as well as a tool for training technicians and students. This second edition incorporates updates on a variety of genetic engineering technologies ranging from microinjection and ES cell transfer to nuclear transfer in a broad range of animal modeling systems. Contains a comprehensive collection of transgenic animal and gene transfer methods Discusses background and introduction to techniques and animal systems Teaches practical step-by-step protocols Fully revised with updates to reflect state-of-the-art technology and associated changes to date
Optimization and Analysis of Single-stranded Oligonucleotide Directed Gene Targeting in Mouse Embryonic Stem Cells
Author: Brian Murphy
Publisher:
ISBN: 9781109985726
Category :
Languages : en
Pages : 106
Book Description
Although correction efficiencies can be highly variable, a model is proposed to separate variables and assist in data analysis. Data is shown to be Poisson distributed. Other technologies such as zinc finger nucleases, RNAi, methylated oligonucleotides, and high throughput screening were examined, but have not boosted efficiencies to the required threshold. The neomycin selectable system is a convenient assay for the testing of new parameters in mES, and these lines may facilitate development of improved ssODN targeting technologies for either animal production or ex vivo gene therapy.
Publisher:
ISBN: 9781109985726
Category :
Languages : en
Pages : 106
Book Description
Although correction efficiencies can be highly variable, a model is proposed to separate variables and assist in data analysis. Data is shown to be Poisson distributed. Other technologies such as zinc finger nucleases, RNAi, methylated oligonucleotides, and high throughput screening were examined, but have not boosted efficiencies to the required threshold. The neomycin selectable system is a convenient assay for the testing of new parameters in mES, and these lines may facilitate development of improved ssODN targeting technologies for either animal production or ex vivo gene therapy.
A Gene Targeting Experiment Designed to Disrupt the NDUFA1 Gene in Mouse Embryonic Stem Cells and the Construction an Initial Characterization of an Inducible Chinese Hamster Cell Line in which the MWFE Protein Can be Selectively Expressed
Author: Toby Houchens
Publisher:
ISBN:
Category :
Languages : en
Pages : 232
Book Description
Publisher:
ISBN:
Category :
Languages : en
Pages : 232
Book Description