Flow Cytometry, Immunohistochemistry, and Molecular Genetics for Hematologic Neoplasms PDF Download
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Author: Tsieh Sun
Publisher: Lippincott Williams & Wilkins
ISBN: 1451180640
Category : Medical
Languages : en
Pages : 449
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Book Description
Immunophenotyping is the most powerful tool in the routine diagnosis of hematologic neoplasms. Immunohistochemical technique is used in histology labs for this purpose, while flow cytometry is used in clinical labs. Although separately these 2 techniques are very useful in detecting lymphomas and leukemias, the combination of both creates a very powerful and definitive diagnostic tool. The addition of molecular genetics to the book makes it an all-encompassing reference text.
Author: Tsieh Sun
Publisher: Lippincott Williams & Wilkins
ISBN: 1451180640
Category : Medical
Languages : en
Pages : 449
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Book Description
Immunophenotyping is the most powerful tool in the routine diagnosis of hematologic neoplasms. Immunohistochemical technique is used in histology labs for this purpose, while flow cytometry is used in clinical labs. Although separately these 2 techniques are very useful in detecting lymphomas and leukemias, the combination of both creates a very powerful and definitive diagnostic tool. The addition of molecular genetics to the book makes it an all-encompassing reference text.
Author: Tsieh Sun
Publisher: Springer Science & Business Media
ISBN: 0387898484
Category : Medical
Languages : en
Pages : 533
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Book Description
Due to its rapid development in recent years, hematopathology has become a very complicated discipline. The current development is mainly in two aspects: the new classification of lymphomas and leukemias and the new techniques. The Revised European-American Classification of Lymphoid Neoplasms (REAL classification) and the World Health Organization (WHO) classification of hematologic neoplasms require not only morphologic criteria but also immunophenotyping and molecular genetics for the diagnosis of hematologic tumors. Immunophenotyping is performed by either flow cytometry or immunohistochemistry. There are many new monoclonal antibodies and new equipments accumulated in recent years that make immunophenotyping more or more accurate and helpful. There are even more new techniques invented in recent years in the field of molecular genetics. In cytogenetics, the conventional karyotype is supplemented and partly replaced by the fluorescence in situ hybridization (FISH) technique. The current development of gene expression profiling is even more powerful in terms of subtyping the hematologic tumors, which may help guiding the treatment and predict the prognosis. In molecular biology, the tedious Southern blotting technique is largely replaced by polymerase chain reaction (PCR). The recent development in reverse-transcriptase PCR and quantitative PCR makes these techniques even more versatile. Because of these new developments, hematopathology has become too complicated to handle by a general pathologist. Many hospitals have to hire a newly trained hematopathologist to oversee peripheral blood, bone marrow and lymph node examinations. These young hematopathologists are geared to the new techniques, but most of them are inexperienced in morphology. No matter how well-trained a hematopathologist is, he or she still needs to see enough cases so that they can recognize the morphology and use the new techniques to substantiate the diagnosis. In other words, morphology is still the basis for the diagnosis of lymphomas and leukemias. Therefore, a good color atlas is the most helpful tool for these young hematopathologists and for the surgical pathologists who may encounter a few cases of hematologic tumors from time to time. In a busy daily practice, it is difficult to refer to a comprehensive hematologic textbook all the time. There are a few hematologic color atlases on the market to show the morphology of the normal blood cells and hematologic tumor cells. These books are helpful but not enough, because tumor cell morphology is variable from case to case and different kinds of tumor cells may look alike and need to be differentiated by other parameters. The best way to learn morphology is through the format of clinical case study. This format is also consistent with the daily practice of hematopathologists and with the pattern in all the specialty board examinations. Therefore, it is a good learning tool for the pathology residents, hematology fellows as well as medical students. This proposed book will present 83 clinical cases with clinical history, morphology of the original specimen and a list of differential diagnoses. This is followed by further testing with pictures to show the test results. At the end, a correct diagnosis is rendered with subsequent brief discussion on how the diagnosis is achieved. A few useful references will be cited and a table will be provided for differential diagnosis in some cases. The major emphasis is the provision of 500 color photos of peripheral blood smears, bone marrow aspirates, core biopsy, lymph node biopsy and biopsies of other solid organs that are involved with lymphomas and leukemias. Pictures of other diagnostic parameters, such as flow cytometric histograms, immunohistochemical stains, cytogenetic karyotypes, fluorescence in situ hybridization and polymerase chain reaction, will also be included. A comprehensive approach with consideration of clinical, morphologic, immunophenotypic and molecular genetic aspects is the best way to achieve a correct diagnosis. After reading this book, the reader will learn to make a diagnosis not only based on the morphology alone but also in conjunction with other parameters.
Author: Tsieh Sun
Publisher: Lippincott Williams & Wilkins
ISBN: 9780781784009
Category : Medical
Languages : en
Pages : 362
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Book Description
This text is a detailed guide to the use of flow cytometry, immunohistochemistry, and molecular genetic techniques for diagnosis of hematologic neoplasms. Dr. Sun explains the principles of these techniques and demonstrates their utility in 39 clinical cases covering all important entities. Each case represents a comprehensive diagnostic approach including a clinical history and flow cytometric, immunohistochemical, and molecular genetic findings. Abundant full-color illustrations show histologic sections, immunohistochemical stains, bone marrow, peripheral blood, and body fluid smears, and each case includes a complete set of flow cytometric histograms. Over 100 tables compare and differentiate the diagnostic features of similar diseases. An image bank will be available on a companion Website.
Author: Anna Porwit
Publisher: Cambridge University Press
ISBN: 1108548830
Category : Medical
Languages : en
Pages : 248
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Book Description
Master implementation of the techniques of flow cytometry in diagnosing complex haematological diseases and malignancies in patients, worldwide. Featuring World Health Organization recommendations on pre-analytical steps, instrument settings and panel construction, this invaluable manual offers invaluable support for those researching, practising and analyzing the cause of hematological malignancies. Authored by leading experts, this book puts flow-cytometry into everyday context. With a focus on multicolour panels, the manual provides readers an experienced understanding of effective, implementation techniques. Practitioners of all levels are offered a background in a variety of diseases presented alongside the most current methodology. Wide-ranging and comprehensive; detailed images of healthy blood, bone marrow and lymph-nodes are illustrated throughout, allowing for effective diagnosis. Through engaging with differential diagnoses, the manual offers an understanding of similar symptoms and mimicking malignancies, avoiding inaccurate results. Featuring in-depth descriptions of chronic diseases; users can reach accurate diagnosis, first time.
Author: Mary Lou Turgeon
Publisher: Jones & Bartlett Learning
ISBN: 1284294498
Category : Medical
Languages : en
Pages : 779
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Book Description
Clinical Hematology: Theory & Procedures, Enhanced Sixth Edition is a competency-based text with built-in study tools to help you master the theory of clinical hematology and the procedures used to diagnose and treat disorders of the blood and bone marrow.
Author: Barbara J. Bain
Publisher: John Wiley & Sons
ISBN: 0470756896
Category : Medical
Languages : en
Pages : 513
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Book Description
Written by one of the world's leading haematologists, and three renowned histopathologists, Bone Marrow Pathology provides a comprehensive guide to the diagnosis of bone marrow disease. Now in its Third Edition, the text has been extensively revised and rewritten to reflect the latest advances in the field. Features: An extremely practical, up-to-the-minute text incorporating the new WHO classification of haematopoietic malignancies A comprehensive text written with great precision and clarity of style Incorporates a new section 'Problems and Pitfalls' - a unique section that will aid the working pathologist faced with a difficult situation An important text for the haematologist, histopathologist and haematopathologist with equal weight given to peripheral blood, aspirate, trephine biology and specialized techniques Extensively illustrated with many of the photographs being of paraffin-embedded sections Combines all the techniques now applied to bone marrow diagnosis, including immunocytochemistry, flow cytometery, immunohistochemistry and the diagnostic role of cytogenetic and molecular genetic analysis
Author: Janet R. Weber
Publisher: Lippincott Williams & Wilkins
ISBN: 1469832224
Category : Medical
Languages : en
Pages : 915
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Book Description
Innovative, systematic, and user-friendly, Health Assessment in Nursing has been acclaimed through four previous editions for the way it successfully helps RN-level students develop the comprehensive knowledge base and expert nursing assessment skills necessary for accurate collection of client data. Maintaining the text’s hallmarks—in-depth, accurate information, a compelling Continuing Case Study, and practical tools that help students develop the skills they need to collect both subjective and objective data—the Fifth Edition now features an exciting array of new chapters, a greater focus on diversity and health assessment through the lifespan, over 150 new illustrations, more than 300 new photos of actual registered nurses and nurse pratitioners performing assessments, and an expanded array of teaching and learning tools.
Author: Timothy Graubert
Publisher:
ISBN: 9780982843512
Category : Hematology
Languages : en
Pages : 637
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Book Description
Hematologists and others working in hematology-related fields need to stay current with the latest advances in the rapidly evolving disciplines of adult and pediatric hematology. The American Society of Hematology Self-Assessment Program (ASH-SAP) is the only complete, comprehensive, educational resource available that fulfills this need, while also providing thorough board and recertification preparation, as well as AMA PRA Category 1 Credit'.
Author: Barbara J. Bain
Publisher: Elsevier Health Sciences
ISBN: 0702069256
Category : Medical
Languages : en
Pages : 599
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Book Description
For more than 65 years, this best-selling text by Drs. Barbara J. Bain, Imelda Bates, and Mike A. Laffan has been the worldwide standard in laboratory haematology. The 12th Edition of Dacie and Lewis Practical Haematology continues the tradition of excellence with thorough coverage of all of the techniques used in the investigation of patients with blood disorders, including the latest technologies as well as traditional manual methods of measurement. You'll find expert discussions of the principles of each test, possible causes of error, and the interpretation and clinical significance of the findings. - A unique section on haematology in under-resourced laboratories. - Ideal as a laboratory reference or as a comprehensive exam study tool. - diagnosis, molecular testing, blood transfusion- and much more. - Complete coverage of the latest advances in the field. - An expanded section on coagulation now covers testing for new anticoagulants and includes clinical applications of the tests.
Author: Liang Cheng
Publisher: Springer
ISBN: 9781461447993
Category : Medical
Languages : en
Pages : 1136
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Book Description
Molecular Genetic Pathology, Second Edition presents up-to-date material containing fundamental information relevant to the clinical practice of molecular genetic pathology. Fully updated in each area and expanded to include identification of new infectious agents (H1N1), new diagnostic biomarkers and biomarkers for targeted cancer therapy. This edition is also expanded to include the many new technologies that have become available in the past few years such as microarray (AmpliChip) and high throughput deep sequencing, which will certainly change the clinical practice of molecular genetic pathology. Part I examines the clinical aspects of molecular biology and technology, genomics. Poharmacogenomics and proteomics, while Part II covers the clinically relevant information of medical genetics, hematology, transfusion medicine, oncology, and forensic pathology. Supplemented with many useful figures and presented in a helpful bullet-point format, Molecular Genetic Pathology, Second Edition provides a unique reference for practicing pathologists, oncologists, internists, and medical genetisists. Furthermore, a book with concise overview of the field and highlights of clinical applications will certainly help those trainees, including pathology residents, genetics residents, molecular pathology fellows, internists, hematology/oncology fellows, and medical technologists in preparing for their board examination/certification.
