Exploring the Mechanisms Controlling Embryogenic Tissue Culture Response and Regeneration Ability in Maize PDF Download
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Author:
Publisher:
ISBN:
Category :
Languages : en
Pages : 174
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Book Description
The ability to form embryogenic cultures and regenerate green plants in vitro is a critical factor in plant genetic engineering, plant propagation and transformation-based research. Maize regeneration ability is genotype-dependent and limiting to tissue culture-based methodologies. The overall goal of this research is to further our understanding of tissue culture response in maize. A backcross-derived mapping population between the highly embryogenic and regenerable maize genotype, A188 was crossed to the poorly culturable maize reference inbred line B73. Near-isogenic lines were screened for tissue culture response. High-resolution mapping in this material to further refine the position of genes on chromosome 3 conferring culture response resulted in two flanking single nucleotide polymorphic markers with a physical distance between markers of 3,053kb or a genetic distance of 4 centimorgans, based on recombination frequency in segregating F2 individuals (n=2243). A fine-mapping experiment revealed highly significant marker-trait associations (P
Author:
Publisher:
ISBN:
Category :
Languages : en
Pages : 174
Get Book Here
Book Description
The ability to form embryogenic cultures and regenerate green plants in vitro is a critical factor in plant genetic engineering, plant propagation and transformation-based research. Maize regeneration ability is genotype-dependent and limiting to tissue culture-based methodologies. The overall goal of this research is to further our understanding of tissue culture response in maize. A backcross-derived mapping population between the highly embryogenic and regenerable maize genotype, A188 was crossed to the poorly culturable maize reference inbred line B73. Near-isogenic lines were screened for tissue culture response. High-resolution mapping in this material to further refine the position of genes on chromosome 3 conferring culture response resulted in two flanking single nucleotide polymorphic markers with a physical distance between markers of 3,053kb or a genetic distance of 4 centimorgans, based on recombination frequency in segregating F2 individuals (n=2243). A fine-mapping experiment revealed highly significant marker-trait associations (P
Author: Frank Lloyd McFarland
Publisher:
ISBN:
Category :
Languages : en
Pages : 0
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Book Description
Maize is one of the most economically important crops in the US and globally for food, feed, and biofuel production. Efficient production of regenerable, embryogenic tissue cultures of maize is necessary for both basic biological research and applied biotechnological approaches to maize genetic improvement. Efficient generation of regenerable cultures in maize is highly genotype-dependent and, historically, researchers in academia and industry have relied extensively upon a restrictively small number of genotypes amenable to conventional tissue culture. To identify the genetic basis for genotypic differences in culture response, a mapping-based approach was utilized. The tissue culture response trait from the regenerable maize inbred A188 was backcrossed into the recalcitrant inbred B73. An A188 genomic introgression spanning 3 Mb on chromosome 3 was strongly associated with regenerable tissue culture response. Three candidate genes were selected in the region based on their expression in A188 immature embryos and functional annotations of genes in the region. A screen of each candidate gene expressed under constitutive promoters identified a single candidate, Wox2a, which was found to induce somatic embryogenesis and embryogenic callus in B73.A complementary approach to mapping the A188 culture response trait was undertaken to map the genotype-dependent response of diverse maize lines to Wuschel2/Baby boom (Wus/Bbm)-mediated genetic transformation. Though Wus/Bbm-mediated transformation reduced recalcitrance of many lines through co-transformation with developmental regulators, strong trends of genotype-dependence were still observed. Ten diverse maize lines were screened to identify lines highly amenable to Wus/Bbm-mediated transformation. Transgenic plants/events were recovered from eight of the ten lines. One line, Gaspé Flint, generated transgenic events efficiently and at a wide range of embryo maturities. A Gaspé Flint x B73 introgression library was screened through Wus/Bbm-mediated transformation and Gaspe̹ Flint introgressions associated with transformability were identified on chromosomes 1, 4, and 7. The scale of the transformation experiments necessitated isolation of thousands of immature embryo explants, for which a high-throughput method of embryo isolation (SIMPLE extraction) was developed. Methods for SIMPLE extraction, as well as optimized methods for Wus/Bbm-mediated and Wox2a-mediated maize transformation were developed to enable easy adoption these developing technologies.
Author: National Research Council
Publisher: National Academies Press
ISBN: 0309166152
Category : Science
Languages : en
Pages : 254
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Book Description
Assists policymakers in evaluating the appropriate scientific methods for detecting unintended changes in food and assessing the potential for adverse health effects from genetically modified products. In this book, the committee recommended that greater scrutiny should be given to foods containing new compounds or unusual amounts of naturally occurring substances, regardless of the method used to create them. The book offers a framework to guide federal agencies in selecting the route of safety assessment. It identifies and recommends several pre- and post-market approaches to guide the assessment of unintended compositional changes that could result from genetically modified foods and research avenues to fill the knowledge gaps.
Author: Annarita Leva
Publisher: BoD – Books on Demand
ISBN: 9535107879
Category : Technology & Engineering
Languages : en
Pages : 224
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Book Description
The purpose of this book is to provide the advances in plant in vitro culture as related to perennial fruit crops and medicinal plants. Basic principles and new techniques, now available, are presented in detail. The book will be of use to researchers, teachers in biotechnology and for individuals interested to the commercial application of plant in vitro culture.
Author: Edwin F. George
Publisher:
ISBN: 9780950932545
Category : Plant micropropagation
Languages : en
Pages :
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Book Description
Author:
Publisher: CIMMYT
ISBN: 9706480412
Category :
Languages : en
Pages : 144
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Book Description
Author: Trevor A. Thorpe
Publisher: Springer Science & Business Media
ISBN: 9401104859
Category : Science
Languages : en
Pages : 560
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Book Description
In vitro Embryogenesis in Plants is the first book devoted exclusively to this topic. As the ultimate demonstration of totipotency in plants, somatic and haploid embryogenesis is of vital importance to all those working on or interested in basic and applied aspects of plantlet information and regeneration. The text includes comprehensive reviews written by experts, on all facts of in vitro and in vivo embryogenesis. Some chapters deal with the morphogenic, structural and developmental, physiological and biochemical, and molecular biological aspects of the subject. Chapters are also devoted to haploid embryogenesis, asexual embryogenesis in nature, zygotic embryogenesis, and zygotic embryo culture. Detailed tables summarizing successful somatic embryogenesis in all vascular plants are also included. This book, therefore, brings together previously scattered information to provide an indispensable reference book for both active researchers, graduate students and anyone interested in this aspect of tissue culture technology and plant development.
Author: Saurabh Bhatia
Publisher: Academic Press
ISBN: 0128024984
Category : Medical
Languages : en
Pages : 452
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Book Description
Modern Applications of Plant Biotechnology in Pharmaceutical Sciences explores advanced techniques in plant biotechnology, their applications to pharmaceutical sciences, and how these methods can lead to more effective, safe, and affordable drugs. The book covers modern approaches in a practical, step-by-step manner, and includes illustrations, examples, and case studies to enhance understanding. Key topics include plant-made pharmaceuticals, classical and non-classical techniques for secondary metabolite production in plant cell culture and their relevance to pharmaceutical science, edible vaccines, novel delivery systems for plant-based products, international industry regulatory guidelines, and more. Readers will find the book to be a comprehensive and valuable resource for the study of modern plant biotechnology approaches and their pharmaceutical applications. Builds upon the basic concepts of cell and plant tissue culture and recombinant DNA technology to better illustrate the modern and potential applications of plant biotechnology to the pharmaceutical sciences Provides detailed yet practical coverage of complex techniques, such as micropropogation, gene transfer, and biosynthesis Examines critical issues of international importance and offers real-life examples and potential solutions
Author: Woong-Young Soh
Publisher: Springer Science & Business Media
ISBN: 9401592535
Category : Science
Languages : en
Pages : 522
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Book Description
This book presents a detailed analysis of up-to-date literature on in vitro morphogenesis at cell, tissue, organ, and whole plant levels. Its driving force is the substantial advances made in the field of morphogenesis in tissue cultures during the last 25 years.
Author: Raúl Alvarez-Venegas
Publisher: Springer
ISBN: 3319079719
Category : Science
Languages : en
Pages : 158
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Book Description
Over the past decades, chromatin remodelling has emerged as an important regulator of gene expression and plant defense. This book provides a detailed understanding of the epigenetic mechanisms involved in plants of agronomic importance. The information presented here is significant because it is expected to provide the knowledge needed to develop in the future treatments to manipulate and selectively activate/inhibit proteins and metabolic pathways to counter pathogens, to treat important diseases and to increase crop productivity. New approaches of this kind and the development of new technologies will certainly increase our knowledge of currently known post-translational modifications and facilitate the understanding of their roles in, for example, host-pathogen interactions and crop productivity. Furthermore, we provide important insight on how the plant epigenome changes in response to developmental or environmental stimuli, how chromatin modifications are established and maintained, to which degree they are used throughout the genome, and how chromatin modifications influence each another.
