Epstein-Barr Virus Protocols PDF Download
Are you looking for read ebook online? Search for your book and save it on your Kindle device, PC, phones or tablets. Download Epstein-Barr Virus Protocols PDF full book. Access full book title Epstein-Barr Virus Protocols by Joanna B. Wilson. Download full books in PDF and EPUB format.
Author: Joanna B. Wilson
Publisher: Springer Science & Business Media
ISBN:
Category : Medical
Languages : en
Pages : 464
Get Book Here
Book Description
Joanna Wilson and Gerhard May have assembled a collection of the key molecular biology protocols used in the analysis of Epstein-Barr virus (EBV), along with a series of valuable immunology, cell biology, and transgenic mouse protocols. These readily reproducible techniques include methods for gene expression with mini-EBV plasmids, for expression analysis by FISH, for EBV detection and quantitation, and for cell proliferation and death assays. In addition, there are EBV-based vectors, an up-to-date map of EBV, a comprehensive table of available latent protein antisera, and assays from in vitro to cell to organ to organism levels. Timely and highly practical, Epstein-Barr Virus Protocols provides powerful tools for elucidating the life cycle of EBV and its host interactions, work that promises the emergence of major new treatments and cures for EBV associated diseases, including several forms of human cancer.
Author: Joanna B. Wilson
Publisher: Springer Science & Business Media
ISBN:
Category : Medical
Languages : en
Pages : 464
Get Book Here
Book Description
Joanna Wilson and Gerhard May have assembled a collection of the key molecular biology protocols used in the analysis of Epstein-Barr virus (EBV), along with a series of valuable immunology, cell biology, and transgenic mouse protocols. These readily reproducible techniques include methods for gene expression with mini-EBV plasmids, for expression analysis by FISH, for EBV detection and quantitation, and for cell proliferation and death assays. In addition, there are EBV-based vectors, an up-to-date map of EBV, a comprehensive table of available latent protein antisera, and assays from in vitro to cell to organ to organism levels. Timely and highly practical, Epstein-Barr Virus Protocols provides powerful tools for elucidating the life cycle of EBV and its host interactions, work that promises the emergence of major new treatments and cures for EBV associated diseases, including several forms of human cancer.
Author: Joanna B. Wilson
Publisher: Humana
ISBN: 9781617371370
Category : Medical
Languages : en
Pages : 0
Get Book Here
Book Description
The discovery of Epstein-Barr virus (EBV) by Epstein, Achong, and Barr, reported in 1964 (Lancet 1:702–703), was stimulated by Denis Burkitt’s rec- nition of a novel African childhood lymphoma and his postulation that an infectious agent was involved in the tumor’s etiology (Nature194:232–234, 1962). Since then, molecular and cellular biological and computational technologies have progressed by leaps and bounds. The advent of recombinant DNA technology opened the possibilities of genetic research more than most would have realized. Not only have the molecular tools permitted the analyses of viral mechanisms, but, importantly, they have formed the basis for discerning viral presence and, subsequently, viral involvement in an increasing number of diseases. Though in every field of science the search for further knowledge is likely to be a limitless phenomenon, the distinct goal in EBV research, namely, to gain sufficient insight into the viral–host interaction to be able to intercept the pathogenic process, is beginning to be realized. Epstein-Barr virus research has effectively entered the postgenomic era that began with the sequencing of the first strains, cloned in the mid to late 1980s.
Author: M. A. Epstein
Publisher: Springer Science & Business Media
ISBN: 3642672361
Category : Medical
Languages : en
Pages : 467
Get Book Here
Book Description
The Epstein-Barr virus was discovered 15 years ago. Since that time an immense body of information has been accumu lated on this agent which has come to assume great signifi cance in many different fields of biological science. Thus, the virus has very special relevance in human medicine and oncology, in tumor virology, in immunology, and in mole cular virology, since it is the cause of infectious mononu cleosis and also the first human cancer virus, etiologically related to endemic Burkitt's lymphoma and probably to nasopharyngeal carcinoma. In addition, continuous human lymphoid cell lines initiated and maintained by the transform ing function of the virus genome provide a laboratory tool with wide and ever-growing applications. Innumerable papers on the Epstein-Barr virus have ap peared over recent years and reports of work with this agent now constitute a veritable flood. The present book provides the first and only comprehensive, authoritative over-view of all aspects of the virus by authors who have been the original and major contributors in their particular disciplines. A complete and up-to-date survey of this unique and important agent is thus provided which should be of great interest to experts, teachers, and students engaged in cancer research, virology, immunology, molecular biology, epide miology, and cell culture. Where topics have been dealt with from more than one of these viewpoints, some inevitable overlap and duplication has resulted; although this has been kept to a minimum, it has been retained in some places because of positive usefulness.
Author: Joanna B. Wilson
Publisher:
ISBN:
Category :
Languages : en
Pages :
Get Book Here
Book Description
The application of recombinant DNA technology to the analysis of Epstein-Barr virus (EBV) is rapidly developing sufficient insight into the virus-host interaction, so that its role in disease pathology is now often discernible and can increasingly be interdicted. In Epstein-Barr Virus Protocols, Joanna Wilson and Gerhard May have assembled a collection of the key molecular biology protocols used in the analysis of Epstein-Barr virus, along with a series of valuable immunology, cell biology, and transgenic mouse protocols. Described in step-by-step detail by experts who use them regularly, these readily reproducible techniques include methods for gene expression with mini-EBV plasmids, for expression analysis by FISH, for EBV detection and quantitation, and for cell proliferation and death assays. In addition, the authors provide information on EBV-based vectors, an up-to-date map of EBV, a comprehensive table of available latent protein antisera, and assays from in vitro to cell to organ to organism levels. Timely and highly practical, Epstein-Barr Virus Protocols provides powerful tools for elucidating the life cycle of EBV and its host interactions, work that promises the emergence of major new treatments and cures for EBV-associated diseases, including several forms of human cancer.
Author: Janos Minarovits
Publisher: Humana
ISBN: 9781493966530
Category : Medical
Languages : en
Pages : 0
Get Book Here
Book Description
This volume explores data from the applications of molecular biological methods and the applications of recent immunological and cytogenetic methods in Epstein-Barr Virus (EBV) that will offer readers possible new solutions to the unresolved problems in the EBV field. Chapters in this book cover topics such as: viral life cycle, latency, EBV-associated diseases and EBV diagnostics; in vitro methods including organotypic cultures for the analysis of EBV-epithelial cell interactions; identification of the interacting viral and cellular proteins using affinity purification-mass spectrometry methods; 3D telomere FISH; transcription analysis using high-throughput RNA sequencing, qPCR and nuclear run-on assay; analysis of viral and cellular microRNAs; isolation and characterization of exosomes and the assessment of their function; characterization of the viral genome by terminal repeat analysis and sequencing; the use of chromatin immunoprecipitation coupled sequencing (ChIP-Seq) for the analysis of Zta-DNA interactions; epigenetic analysis by bisulfite sequencing and ChIP; novel in vivo models for the study of EBV infection; and how immunological, virological, tissue culture and molecular methods can be combined to yield Good Manufacturing Practice-compliant EBV-specific T cells for the immunotherapy of EBV-associated post-transplant lymphoproliferative diseases (PTLD). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Epstein Barr-Virus: Methods and Protocols is a valuable resource for anyone who is interested in this fascinating and evolving field.
Author: John M. Walker
Publisher:
ISBN:
Category : Molecular biology -- v.236. Plant functional genomics -- v.237. G protein signaling -- v.238. Biopolymer methods in tissue engineering -- v.239. Cell migration in inflammation and immunity -- v.240. Mammalian artificial chromosomes -- v.241. Cell cycle checkpoint control protocols -- v.242. Atomic force microscopy -- v.243. Chiral separations: Methods and protocols -- v.244. Protein purification protocols -- v.245-6. Gene delivery to mammalian cells -- v.247. Drosophila cytogenetics protocols -- v.248. Antibody engineering -- v.249. Cytokine protocols -- v.251. HPLC of peptides and proteins: Methods and protocols -- v.265. RNA interference, editing, and modification -- v.274. Photosynthesis research protocols -- v.318. Plant cell culture protocols -- v.323. Arabidopsis protocols
Languages : en
Pages :
Get Book Here
Book Description
Author: Ann Arvin
Publisher: Cambridge University Press
ISBN: 1139461648
Category : Medical
Languages : en
Pages : 1325
Get Book Here
Book Description
This comprehensive account of the human herpesviruses provides an encyclopedic overview of their basic virology and clinical manifestations. This group of viruses includes human simplex type 1 and 2, Epstein–Barr virus, Kaposi's Sarcoma-associated herpesvirus, cytomegalovirus, HHV6A, 6B and 7, and varicella-zoster virus. The viral diseases and cancers they cause are significant and often recurrent. Their prevalence in the developed world accounts for a major burden of disease, and as a result there is a great deal of research into the pathophysiology of infection and immunobiology. Another important area covered within this volume concerns antiviral therapy and the development of vaccines. All these aspects are covered in depth, both scientifically and in terms of clinical guidelines for patient care. The text is illustrated generously throughout and is fully referenced to the latest research and developments.
Author:
Publisher: ScholarlyEditions
ISBN: 1464945373
Category : Medical
Languages : en
Pages : 34
Get Book Here
Book Description
Epstein-Barr Virus Infections: Advances in Research and Treatment: 2011 Edition is a ScholarlyPaper™ that delivers timely, authoritative, and intensively focused information about Epstein-Barr Virus Infections in a compact format. The editors have built Epstein-Barr Virus Infections: Advances in Research and Treatment: 2011 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Epstein-Barr Virus Infections in this eBook to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Epstein-Barr Virus Infections: Advances in Research and Treatment: 2011 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.
Author: Kasia Kines
Publisher:
ISBN: 9781722895365
Category :
Languages : en
Pages : 586
Get Book Here
Book Description
From the Best Selling author Dr. Kasia Kines. Take your life back! For anyone suffering from Epstein-Barr Virus (EBV), chronic fatigue, autoimmune disorders, Infectious Mononucleosis, or other mystery illnesses, this is a must read!Do you feel lost and hopeless, living a life of illness with no answers? Do you feel unsupported by your medical team, left to suffer alone with no guidance? Are you searching for solutions to get your health and life back on track?Finally, a book that speaks to both the medical community and people inflicted by both mono and chronic conditions caused by EBV!The expanse of this pivotal book is far reaching - many people may not yet know they have been infected with EBV (commonly known as the "kissing disease"), but yet they struggle every day with chronic symptoms such as severe brain fog, debilitating fatigue, headaches, fever, chills, sore throat, body aches, and abdominal discomfort and can't seem to find a successful treatment solution.Eventually, some go on to develop autoimmune disorders, e.g. Lupus, Multiple Sclerosis, or Hashimoto's Thyroiditis and even cancer and are told that their immune system is simply confused and is now attacking itself, left with no solutions or hope for recovery.Alarmingly, chronic EBV infection is almost completely missed in medical practice, and as a result of never being tested, misinterpreting the test results, not being diagnosed, or being mis-diagnosed by their doctors, millions of people suffer debilitating conditions for many years with no answers.But this suffering can be avoided. And with this book, answers can be found.Author Dr. Kasia Kines is considered by her colleagues to be "one of the most brilliant, and importantly, clinically experienced functional nutritionists in the world." In Epstein-Barr Virus Solution, Dr. Kines provides a groundbreaking examination of EBV and not only how it causes chronic fatigue, autoimmune disorders, and even cancer but also how recovery is possible. This book was written in hopes that it will globally change the way EBV is understood, diagnosed, and treated.The deep journey into the existing research sheds light on the EBV virus for both the seasoned medical community and the layperson. Along with the validated research, Epstein-Barr Virus Solution presents practical, time-tested, and evidence-based clinical solutions for the testing and medical nutrition therapy for EBV, testing and lab interpretation, unique supplementation protocols, recovery strategies for stress, insomnia, fatigue, and toxicity, pantry and kitchen rehab for EBV, and delicious anti-EBV recipes. Through Dr. Kines' compassionate guidance, readers will feel confident to discuss their concerns with their doctor, to ask for testing, and to take their lives back from EBV. Epstein-Barr Virus Solution urges medical providers to hear their clients' concerns and provides the medical community with the research and insights needed to avoid misdiagnosis and common problems with testing for EBV.Epstein-Barr Virus Solution was made possible because of Dr. Kines' long-standing expertise as a functional clinical nutritionist who has dedicated her practice to working with clients inflicted by EBV. Dr. Kines tells readers in easy-to-understand language that recovery and hope for better health are possible through targeted nutrition and lifestyle changes. Readers are given a toolkit full of practical advice and guidance, including exclusive materials from Dr. Kines' life-changing 30-Day Detox Program that she has taught successfully for over 10 years.The research is out there, and Dr. Kasia Kines has found it, synthesized it, and utilized it to implement successful treatment protocols in her own clinic. Clients who had suffered years with chronic EBV infection symptoms are now recovering and living their lives.This book will provide those infected with EBV everything they need to finally take control of their health and take their life and happiness back.
Author: Thomas Tursz
Publisher: John Libbey Eurotext
ISBN: 9782742000081
Category : Epstein-Barr virus
Languages : en
Pages : 870
Get Book Here
Book Description
Text in English & French. This book gathers together all the most recent world-wide knowledge in the various fields of research into the Epstein-Barr virus (EBV), from the most fundamental subjects (virology, molecular and cellular biology and immunology) through epidemiology, pathological anatomy, clinical research and therapeutics. Featured in the book are highly original chapters dealing with malignant lymphomas in AIDS patients, detection and prevention in developing countries, formulation of an anti-EBV vaccine. This comprehensive and clearly written book is a valuable and essential tool for all researchers and clinicians.
