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Author: D Schrafnagel
Publisher: CRC Press
ISBN: 9780824783198
Category : Science
Languages : en
Pages : 646
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Book Description
Documents the enormous contribution electron microscopy has made to the study of lung biology, describing new analytical instruments, recent technological developments, and future avenues of research. Illustrated with 290 micrographs of normal and abnormal lung, rare tumors, and other features of lu
Author: D Schrafnagel
Publisher: CRC Press
ISBN: 9780824783198
Category : Science
Languages : en
Pages : 646
Get Book Here
Book Description
Documents the enormous contribution electron microscopy has made to the study of lung biology, describing new analytical instruments, recent technological developments, and future avenues of research. Illustrated with 290 micrographs of normal and abnormal lung, rare tumors, and other features of lu
Author: Pier C Braga
Publisher:
ISBN: 9788847022560
Category :
Languages : en
Pages : 116
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Book Description
Author: Joan Gil
Publisher: CRC Press
ISBN: 1000147401
Category : Medical
Languages : en
Pages : 1026
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Book Description
This research-level reference provides a review of the morphological techniques that have become a primary method of anatomical study correlating structure and function in lung physiology and pathology. Detailing the evolution of anatomy as a research discipline, it explores general structural techn
Author: Gonzague Kistler
Publisher:
ISBN:
Category :
Languages : en
Pages : 24
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Book Description
Author: Eduard Klika
Publisher:
ISBN:
Category : Microscopes électroniques
Languages : en
Pages : 132
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Book Description
Studie zkoumá žábry ryb a plicní tkáň žáby, želvy, slepice, ježka, krysy, králíka, kočky, opice a člověka. Aplikuje jak komplexní elektronové mikroskopické a histochemické vyšetření, tak metodu plošných preparátů.
Author: Gregory Lee Finch
Publisher:
ISBN:
Category :
Languages : en
Pages : 402
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Book Description
Author: C. Torre
Publisher:
ISBN:
Category : Chronic pulmonary emphysema
Languages : en
Pages : 6
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Book Description
The authors describe the results obtained using the scanning electron microscope (SEM) to study drowned lungs and control lungs. Results are compared with a previous study carried out using laboratory animals. The results from both studies are analogous, in the absence of chronic pulmonary emphysema. The diagnosis of chronic pulmonary emphysema is simple with the SEM even when dealing with autoptic material obtained 24 to 48 h after death, but the diagnosis of drowning becomes difficult in lungs with chronic emphysema.
Author: Mary Jane Stafford
Publisher:
ISBN:
Category : Anoxemia
Languages : en
Pages : 104
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Book Description
Author: Stefan Uhlig
Publisher: Springer Science & Business Media
ISBN: 9783764354275
Category : Medical
Languages : en
Pages : 576
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Book Description
Airways.- 1 Measurement of lung function in rodents in vivo.- Spontaneous respiration.- Pulmonary manoeuvres.- Material and equipment.- Lung function laboratory.- Methods.- Preparation and calibration.- Pulmonary function testing.- Examples for applications.- Discussion.- Troubleshooting.- References.- 2 The isolated perfused lung.- Advantages and disadvantages of perfused lungs.- Theoretical background.- Vascular resistance.- Respiratory mechanics.- Material and equipment.- Artificial thorax chamber and ventilation.- Perfusion.- Weight measurement.- Gas exchange.- Methods.- Surgery and setting up the lung.- Criteria for viability.- Cleaning the apparatus.- An example application.- Discussion.- Interpretation of the results.- Constant flow (CFP) versus constant pressure perfusion (CPP).- Negative or positive pressure ventilation.- Choice of perfusate.- Recirculating versus non-recirculating perfusion.- Additional experimental options.- Troubleshooting.- Final comments.- References.- 3 Lung explants.- Material and equipment.- Preparation of culture media.- Preparation of agarose.- Preparation of animals.- Preparation of explants.- Image acquisition.- Variations on this technique.- Applications.- Effects of bronchoconstriction.- Measurements of mucociliary clearance.- Measurements of pulmonary vasculature.- Long term explant culture techniques.- Investigations of protein and gene expression.- Troubleshooting.- Discussion.- Acknowledgements.- References.- 4 Tracheal preparations.- Methods.- Guinea pig tracheal preparations.- Immersion techniques.- Tracheal chain.- Spirally cut trachea.- Zig-zag tracheal strip.- Tracheal tube preparations.- Superfusion techniques.- Electrically stimulated trachea.- Epithelium-denuded trachea.- Conclusion.- References.- Vessels.- 5 Intravital microscopy: Airway circulation.- Materials and equipment.- Microscope.- Video equipment.- Peripheral equipment.- Ventilation.- Solutions.- Methods.- Surgery.- Experimental procedure.- Species differences.- Discussion.- References.- 6 The bronchial circulation.- Importance and role of the bronchial circulation.- Postobstructive pulmonary vasculopathy (POPV) and principles of the techniques.- Material and equipment.- Production of POPV in dogs, rats and guinea pigs: Ligation of the left main pulmonary artery.- In situ perfused LLL preparation.- Morphological assessment of the bronchial and pulmonary vasculature using light microscopy and morphometry.- Methods.- Surgical ligation of the left main pulmonary artery in dogs, rats and guinea pigs.- Canine model.- Rat and guinea pig model.- In situ perfused LLL preparation to measure pulmonary and bronchial vascular flows, pressures and resistances using modified AO and VO and bronchial vascular micropuncture.- Procedure for the in situ perfused LLL preparation.- AO and VO measurements.- Modified in situ perfused LLL preparation for bronchial collateral.- vascular pressure measurements by micropuncture.- Morphological assessment of the bronchial and pulmonary vasculature, using light microscopy and morphometry.- Measurement of pulmonary vascular medial thickness and muscularization in lungs injected with pigmented gelatin-barium mixtures.- Fixation and preparation.- Morphometry.- Assessment of proliferation in the bronchial vasculature.- Bronchial vessel number per airway.- Assessment of bronchial vascular endothelial proliferation using bromodeoxyuridine (BrdU) labeling.- Discussion and troubleshooting.- Production of POPV.- In situ perfused left lower lobar preparation.- Morphological assessment of the bronchial and pulmonary vasculature.- Acknowledgements.- References.- 7 Segmental vascular resistance and compliance from vascular occlusion.- Methods.- The lumped parameter RCR model.- The continuous RC distribution.- More distributed lumped parameter models.- The 3C4R model.- The 3C2R model.- Arterial occlusion in vivo.- Acknowledgements.- References.- Edema.- 8 Experimental and clinical measurement of pulmonary ...
Author: John Stirling
Publisher: John Wiley & Sons
ISBN: 1119973996
Category : Science
Languages : en
Pages : 492
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Book Description
Diagnostic Electron Microscopy Diagnostic Electron Microscopy: A Practical Guide to Interpretation and Technique summarises the current interpretational applications of TEM in diagnostic pathology. This concise and accessible volume provides a working guide to the main, or most useful, applications of the technique including practical topics of concern to laboratory scientists, brief guides to traditional tissue and microbiological preparation techniques, microwave processing, digital imaging and measurement uncertainty. The text features both a screening and interpretational guide for TEM diagnostic applications and current TEM diagnostic tissue preparation methods pertinent to all clinical electron microscope units worldwide. Containing high-quality representative images, this up-to-date text includes detailed information on the most important diagnostic applications of transmission electron microscopy as well as instructions for specific tissues and current basic preparative techniques. The book is relevant to trainee pathologists and practising pathologists who are expected to understand and evaluate/screen tissues by TEM. In addition, technical and scientific staff involved in tissue preparation and diagnostic tissue evaluation/screening by TEM will find this text useful.
