Discovery of Bioactive Natural Products from Actinomycetes by Inactivation and Heterologous Expression of Biosynthetic Gene Clusters PDF Download
Are you looking for read ebook online? Search for your book and save it on your Kindle device, PC, phones or tablets. Download Discovery of Bioactive Natural Products from Actinomycetes by Inactivation and Heterologous Expression of Biosynthetic Gene Clusters PDF full book. Access full book title Discovery of Bioactive Natural Products from Actinomycetes by Inactivation and Heterologous Expression of Biosynthetic Gene Clusters by Jing Zhu. Download full books in PDF and EPUB format.
Author: Jing Zhu
Publisher:
ISBN:
Category :
Languages : en
Pages :
Get Book Here
Book Description
Author: Jing Zhu
Publisher:
ISBN:
Category :
Languages : en
Pages :
Get Book Here
Book Description
Author: Ravishankar V. Rai
Publisher: Springer Nature
ISBN: 9811661324
Category : Science
Languages : en
Pages : 512
Get Book Here
Book Description
This book provides in-depth information about the ecology, diversity and applications of Actinomycetes. The book is divided into two major parts. The first part discusses the diversity, chemical biology and ecology of Actinomycetes. It also covers the discovery of natural products from soil, endophytic and marine-derived Actinomycetes. It includes natural product discovery, chemical biology, new methods for discovering secondary metabolites, structure elucidation and biosynthetic research of natural products. The chapters in this part focus on the effects of biological and chemical elicitation at molecular level on secondary metabolism in Actinomycetes. The second part of the book discusses genomic and synthetic biology approaches in Actinomycetes drug discovery. This part includes chapters focused on the application of metabolic engineering to optimize natural product synthesis and the use of omics data in engineering of regulatory genes. It covers the advanced tools of synthetic biology and metabolic engineering including cluster assembly, CRISPR/Cas9 technologies, and chassis strain development for natural product overproduction in Actinomycetes. It describes the use of bioinformatics tools for reprogramming of biosynthetic pathways through polyketide synthase and non-ribosomal peptide synthetase engineering. These advanced genomic and molecular tools are expected to accelerate the discovery and development of new natural products from Actinomycetes with medicinal and other industrial applications. The book is useful to researchers and students in the field of microbiology, pharmaceutical sciences and drug discovery.
Author: Jun Lin
Publisher:
ISBN:
Category :
Languages : de
Pages :
Get Book Here
Book Description
Heterologous expression of natural product biosynthetic pathways is of rising interest to fungal bioactive compound discovery and engineering. However, most eukaryotic genes are regulated by individual regulatory elements and transcribed as monocistronic mRNA. Therefore, regardless of plasmid transformation or chromosomal integration of DNA, reconstruction and expression of biosynthetic gene clusters in the eukaryotic recipient require improved assembling pipeline of vectors and coordinated control of expression of multiple genes. Moreover, valuable medicine biosynthesis based on heterologous expression remains an ongoing challenge owing to the strict metabolic regulation in the chosen host. To circumvent these obstacles, a specialized vector was designed, for flexible but modularized construction of multiple genes. In the scheme, TEV protease and its recognition site combined with a previously documented P2A-mediated expression system, allow expression of eukaryotic genes in a “polycistronic” mRNA by co-translational and post-translational cleavages. As another highlight, the split fluorescent protein was introduced for visualizing and rapid screening of fungal transformants. On the basis of this new tool, the austinoid biosynthetic pathway has been rewired successfully in A. nidulans. Also, it represents the first-time producing psilocybin heterologously using fermenting condition, from which the high titer 15.4 mg/g (110 mg/L) was achieved. Subsequently, the de-branching strategy was beneficial for the yield of psilocybin, which even lifted to 57 mg/g in the yeast cell-based factory. Meanwhile, the Trp auxotrophic strain has been endowed with the capability to synthesize isotope-labeled psilocybin in an atom-economic manner.
Author: Dharumadurai Dhanasekaran
Publisher: BoD – Books on Demand
ISBN: 9535122487
Category : Science
Languages : en
Pages : 401
Get Book Here
Book Description
This book presents an introductory overview of Actinobacteria with three main divisions: taxonomic principles, bioprospecting, and agriculture and industrial utility, which covers isolation, cultivation methods, and identification of Actinobacteria and production and biotechnological potential of antibacterial compounds and enzymes from Actinobacteria. Moreover, this book also provides a comprehensive account on plant growth-promoting (PGP) and pollutant degrading ability of Actinobacteria and the exploitation of Actinobacteria as ecofriendly nanofactories for biosynthesis of nanoparticles, such as gold and silver. This book will be beneficial for the graduate students, teachers, researchers, biotechnologists, and other professionals, who are interested to fortify and expand their knowledge about Actinobacteria in the field of Microbiology, Biotechnology, Biomedical Science, Plant Science, Agriculture, Plant pathology, Environmental Science, etc.
Author: Christopher T. Walsh
Publisher: Royal Society of Chemistry
ISBN: 1788010760
Category : Science
Languages : en
Pages : 787
Get Book Here
Book Description
This textbook describes the types of natural products, the biosynthetic pathways that enable the production of these molecules, and an update on the discovery of novel products in the post-genomic era.
Author: Vijai Singh
Publisher: Springer Nature
ISBN: 9811500819
Category : Science
Languages : en
Pages : 349
Get Book Here
Book Description
This book addresses the design of emerging conceptual tools, technologies and systems including novel synthetic parts, devices, circuits, oscillators, biological gates, and small regulatory RNAs (riboregulators and riboswitches), which serve as versatile control elements for regulating gene expression. Synthetic biology, a rapidly growing field that involves the application of engineering principles in biology, is now being used to develop novel systems for a wide range of applications including diagnostics, cell reprogramming, therapeutics, enzymes, vaccines, biomaterials, biofuels, fine chemicals and many more. The book subsequently summarizes recent developments in technologies for assembling synthetic genomes, minimal genomes, synthetic biology toolboxes, CRISPR-Cas systems, cell-free protein synthesis systems and microfluidics. Accordingly, it offers a valuable resource not only for beginners in synthetic biology, but also for researchers, students, scientists, clinicians, stakeholders and policymakers interested in the potential held by synthetic biology.
Author: Tobias Kieser
Publisher:
ISBN: 9780708406236
Category : Bacterial genetics
Languages : en
Pages : 613
Get Book Here
Book Description
Author: Frank E. Koehn
Publisher: Springer Science & Business Media
ISBN: 1461446546
Category : Medical
Languages : en
Pages : 248
Get Book Here
Book Description
This volume will cover the natural products as they relate to cancer chemotherapy. The topics will include history and current status , recent launches, new clinical candidates and approved drugs directly derived from natural products, current and future cancer target opportunities for natural products, leveraging natural products as tools for new target generation , new approaches to cancer drug discovery through natural products based lead generation, and enabling technologies which leverage the unique attributes of natural products.
Author: Joachim Wink
Publisher: Springer
ISBN: 3319603396
Category : Science
Languages : en
Pages : 398
Get Book Here
Book Description
This book provides in-depth insights into the biology, taxonomy, genetics, physiology and biotechnological applications of Actinobacteria. It especially focuses on the latter, reviewing the wide variety of actinobacterial bioactive molecules and their benefits for diverse industrial applications such as agriculture, aquaculture, biofuel production and food technology. Actinobacteria are one of the most promising sources of small bioactive molecules and it is estimated that only a small percentage of actinobacterial bioactive chemicals have been discovered to date. Identifying new diverse gene clusters of biotechnological relevance in the genome of Actinobacteria will be crucial to developing advanced applications for pharmaceutical, industrial and agricultural purposes. The book offers a unique resource for all graduate students, researchers and practitioners in the fields of microbiology, microbial biotechnology, and the genetic engineering of Actinobacteria.
Author: Nancy P. Keller
Publisher: Humana Press
ISBN: 9781627031219
Category : Science
Languages : en
Pages : 288
Get Book Here
Book Description
Filamentous fungi have long been known for their ability to produce an enormous range of unusual chemical compounds known as secondary metabolites, many of which have potentially useful antibiotic or pharmacological properties. Recent focus on fungal genomics coupled with advances in detection and molecular manipulation techniques has galvanized a revitalization of this field. Fungal Secondary Metabolism: Methods and Protocols is aimed at providing the key methodologies currently in use and necessary for accessing and exploiting the natural product information provided by the genomes of this large and varied kingdom. Written by active researchers in the field, the chapters deal with all the steps necessary, from optimization of fungal culture conditions for metabolite production, through rapid genome sequencing and bioinformatics, and genetic manipulations for functional analysis, to detection and testing of metabolites. In addition, chapters on basic science address approaches to the genetic regulation, protein biochemistry, and cellular localization of the biosynthetic pathways. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and hands-on, Fungal Secondary Metabolism: Methods and Protocols encourages new investigators to enter the field and expands upon the expertise and range of skills of those already researching fungal natural products.
