Diffusion in Crowded Environment of Live Cells PDF Download
Are you looking for read ebook online? Search for your book and save it on your Kindle device, PC, phones or tablets. Download Diffusion in Crowded Environment of Live Cells PDF full book. Access full book title Diffusion in Crowded Environment of Live Cells by Michael Charles Konopka. Download full books in PDF and EPUB format.
Author: Michael Charles Konopka
Publisher:
ISBN:
Category :
Languages : en
Pages : 198
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Book Description
Author: Michael Charles Konopka
Publisher:
ISBN:
Category :
Languages : en
Pages : 198
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Book Description
Author: Apratim Dhar
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Book Description
Biomolecular dynamics and stability are predominantly investigated in vitro, and extrapolated to explain function in the living cell. In this thesis, we attempt to bridge this divide by performing studies of protein folding in in vitro crowded environments and in living cells. We begin by investigating the thermodynamic and kinetic behavior of the proteins in protein/carbohydrate matrices, and find significant differences compared to dilute, buffer solutions. We then develop Fast Relaxation Imaging (FReI) as a novel imaging technique to investigate protein folding dynamics inside living cells with millisecond temporal resolution and micrometer spatial resolution. We study the folding of the metabolic enzyme phosphoglycerate kinase (PGK) in U2OS (human osteosarcoma) cells and observe variations in folding kinetics within a single cell. FReI experiments carried out on an ensemble of 30 cells reveal large variations in folding time from cell to cell, which are analyzed to estimate the folding diffusion coefficient in vivo. Finally, we use short peptide tags to target PGK to the nucleus and endoplasmic reticulum of live cells and discern differential folding stability and kinetics in these organelles compared to protein folding in the cytoplasm.
Author: Ron Milo
Publisher: Garland Science
ISBN: 1317230698
Category : Science
Languages : en
Pages : 400
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Book Description
A Top 25 CHOICE 2016 Title, and recipient of the CHOICE Outstanding Academic Title (OAT) Award. How much energy is released in ATP hydrolysis? How many mRNAs are in a cell? How genetically similar are two random people? What is faster, transcription or translation?Cell Biology by the Numbers explores these questions and dozens of others provid
Author: Luiz Roberto Evangelista
Publisher: Springer Nature
ISBN: 3031181506
Category : Science
Languages : en
Pages : 411
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Book Description
This book provides a contemporary treatment of the problems related to anomalous diffusion and anomalous relaxation. It collects and promotes unprecedented applications dealing with diffusion problems and surface effects, adsorption-desorption phenomena, memory effects, reaction-diffusion equations, and relaxation in constrained structures of classical and quantum processes. The topics covered by the book are of current interest and comprehensive range, including concepts in diffusion and stochastic physics, random walks, and elements of fractional calculus. They are accompanied by a detailed exposition of the mathematical techniques intended to serve the reader as a tool to handle modern boundary value problems. This self-contained text can be used as a reference source for graduates and researchers working in applied mathematics, physics of complex systems and fluids, condensed matter physics, statistical physics, chemistry, chemical and electrical engineering, biology, and many others.
Author: Alexander Shekhtman
Publisher:
ISBN: 9783039282555
Category : Biology (General)
Languages : en
Pages : 152
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Book Description
This Special Issue examines state-of-the-art in-cell NMR spectroscopy as it relates to biological systems of increasing complexity. The compendia of research and recent innovations from prominent laboratories in the field of solid state and solution in-cell NMR spectroscopy, metabolomics and technology development are presented. The work establishes in-cell NMR spectroscopy as the premier method for determining the structures and interaction capabilities of biological molecules at high resolution within the delicately intricate interior of living cells, and the means of utilizing cells as living laboratories to directly assess the effects of exogenous and endogenous stimuli on cell physiology.].
Author:
Publisher: Academic Press
ISBN: 0123918693
Category : Science
Languages : en
Pages : 573
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Book Description
This volume of Methods in Enzymology is the first of three parts looking at current methodology for the imaging and spectroscopic analysis of live cells. The chapters provide hints and tricks not available in primary research publications. It is an invaluable resource for academics, researchers and students alike. - Expert authors who are leaders in the field - Extensively referenced and useful figures and tables - Provides hints and tricks to facilitate reproduction of methods
Author: Swomitra Palit
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Book Description
Macromolecular crowding and confinement, the effects caused by high concentrations of macromolecules in solution and/or in small compartments, are believed to influence diffusion processes, intermolecular interactions, protein folding, and intracellular transport in living cells. Understanding mechanisms of transport in biological systems (such as living cells) is complex and challenging. We construct cell mimetic environments in which the artificial macromolecules (e.g. polyethylene glycol, Ficoll70) are compartmentalized not in cells but in concentrated environments and agarose gel networks. In this work we have established a system to generate stable and monodisperse droplets of hierarchical confinement. The goal of this study is to measure translational diffusion in crowded and confined geometries of varying concentrations of different macromolecules on diffusion. We have combined the use of pulsed-fieldgradient nuclear magnetic resonance (PFG NMR) with small-angle neutron scattering (SANS) in order to obtain new insights in simple model systems of macromolecular crowding. The NMR and SANS techniques complement each other. Using PFG NMR technique, we have monitored the dynamics of synthetic macromolecules with multiple chemical components in complex environments. SANS, on the other hand, yields structure (size) of macromolecules. Our experimental findings in cell mimetic environments provide an important step towards gaining further insights into the effects of macromolecular crowding on diffusion and conformation.
Author: Peter Schuck
Publisher: Springer Science & Business Media
ISBN: 0387359664
Category : Science
Languages : en
Pages : 537
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Book Description
This volume successfully and clearly examines how biophysical approaches can be used to study complex systems of reversibly interacting proteins. It deals with the methodology behind the research and shows how to synergistically incorporate several methodologies for use. Each chapter treats and introduces the reader to different biological systems, includes a brief summary of the physical principles, and mentions practical requirements.
Author: Yutaka Ito
Publisher: Royal Society of Chemistry
ISBN: 1839160934
Category : Science
Languages : en
Pages : 322
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Book Description
In-cell NMR spectroscopy is a relatively new field. Despite its short history, recent in-cell NMR-related publications in major journals indicate that this method is receiving significant general attention. This book provides the first informative work specifically focused on in-cell NMR. It details the historical background of in-cell NMR, host cells for in-cell NMR studies, methods for in-cell biological techniques and NMR spectroscopy, applications, and future perspectives. Researchers in biochemistry, biophysics, molecular biology, cell biology, structural biology as well as NMR analysts interested in biological applications will all find this book valuable reading.
Author: Luiz Roberto Evangelista
Publisher: Cambridge University Press
ISBN: 1108663486
Category : Science
Languages : en
Pages : 361
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Book Description
Anomalous diffusion has been detected in a wide variety of scenarios, from fractal media, systems with memory, transport processes in porous media, to fluctuations of financial markets, tumour growth, and complex fluids. Providing a contemporary treatment of this process, this book examines the recent literature on anomalous diffusion and covers a rich class of problems in which surface effects are important, offering detailed mathematical tools of usual and fractional calculus for a wide audience of scientists and graduate students in physics, mathematics, chemistry and engineering. Including the basic mathematical tools needed to understand the rules for operating with the fractional derivatives and fractional differential equations, this self-contained text presents the possibility of using fractional diffusion equations with anomalous diffusion phenomena to propose powerful mathematical models for a large variety of fundamental and practical problems in a fast-growing field of research.
