Molecular and Diagnostic Procedures in Mycoplasmology PDF Download
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Author:
Publisher: Academic Press
ISBN: 0080536794
Category : Science
Languages : en
Pages : 489
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Book Description
This book and its companion, Volume I, concentrate on new procedures--especially those based on the new molecular methodology--developed within the past decade. This volume deals with the new genetic and immunological tools applied to the diagnosis of mycoplasma infections of humans, animals, plants, insects, and all cultures. Volume I outlines the approaches, techniques, and procedures applied to cell and molecular biology studies of mycoplasmas. - Diagnostic genetic probes - Immunological tools - Antibiotic sensitivity testing - Diagnosis of specific diseases - Experimental infections - Diagnosis of mycoplasma infections of cell cultures
Author:
Publisher: Academic Press
ISBN: 0080536794
Category : Science
Languages : en
Pages : 489
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Book Description
This book and its companion, Volume I, concentrate on new procedures--especially those based on the new molecular methodology--developed within the past decade. This volume deals with the new genetic and immunological tools applied to the diagnosis of mycoplasma infections of humans, animals, plants, insects, and all cultures. Volume I outlines the approaches, techniques, and procedures applied to cell and molecular biology studies of mycoplasmas. - Diagnostic genetic probes - Immunological tools - Antibiotic sensitivity testing - Diagnosis of specific diseases - Experimental infections - Diagnosis of mycoplasma infections of cell cultures
Author: Joseph G. Tully
Publisher:
ISBN:
Category : Microbiology
Languages : en
Pages : 468
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Book Description
Author: Joseph Tully
Publisher: Elsevier
ISBN: 0323149391
Category : Medical
Languages : en
Pages : 465
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Book Description
Methods in Mycoplasmology, Volume II: Diagnostic Mycoplasmology is the second of a two-volume series that resulted from cumulative efforts to meet the need for standardized techniques in mycoplasmology. The book is organized into five sections. Section A discusses techniques for isolating mycoplasmas from humans. These include the recovery of mycoplasmas from the respiratory and genitourinary tracts, from blood and special tissues, and serological Identification of mycoplasmas from humans. Section B focuses on techniques for isolating mycoplasmas from animals, including the recovery and identification of avian, bovine, caprine, ovine, canine, and feline mycoplasmas. Section C presents techniques for isolating mycoplasmas from cell cultures. These include agar and broth techniques, the use of indicator cell lines, and DNA fluorochrome staining. Section D discusses techniques for isolating mycoplasmas from plants and insects. Section E describes some of the techniques that are useful in evaluating the pathogenicity of mycoplasmas, ureaplasmas, and spiroplasmas. Section F defines some of the techniques useful in virus detection by electron microscopy and presents methods for isolating and characterizing viruses from both mycoplasmas and spiroplasmas.
Author: Shmuel Razin
Publisher: Springer Science & Business Media
ISBN: 0306476061
Category : Science
Languages : en
Pages : 574
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Book Description
was the result of the efforts of Robert Cleverdon. The rapidly developing discipline of molecular biology and the rapidly expanding knowledge of the PPLO were brought together at this meeting. In addition to the PPLO specialists, the conference invited Julius Marmur to compare PPLO DNA to DNA of other organisms; David Garfinkel, who was one of the first to develop computer models of metabolism; Cyrus Levinthal to talk about coding; and Henry Quastler to discuss information theory constraints on very small cells. The conference was an announcement of the role of PPLO in the fundamental understanding of molecular biology. Looking back 40-some years to the Connecticut meeting, it was a rather bold enterprise. The meeting was international and inter-disciplinary and began a series of important collaborations with influences resonating down to the present. If I may be allowed a personal remark, it was where I first met Shmuel Razin, who has been a leading figure in the emerging mycoplasma research and a good friend. This present volume is in some ways the fulfillment of the promise of that early meeting. It is an example of the collaborative work of scientists in building an understanding of fundamental aspects of biology.
Author: Samuel Razin
Publisher: Elsevier
ISBN: 0323147135
Category : Nature
Languages : en
Pages : 527
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Book Description
Methods in Mycoplasmology, Volume I: Mycoplasma Characterization is the first of a two-volume series that resulted from cumulative efforts to meet the need for standardized techniques in mycoplasmology. The book is organized into five sections. Section A provides an introduction to the mycoplasmas by discussing their characteristics and classification, mycoplasma-animal host interrelationships, and mycoplasma-plant-insect interrelationships. The chapters in Section B examine the morphological and ultrastructural features of mycoplasmas, using techniques such as phase-contrast microscopy, dark-field microscopy, and transmission electron microscopy. Section C discusses the cultivation and nutrition of mycoplasmas. Section D focuses on analytical methods for the characterization of mycoplasmas, including harvest and washing procedures, cell lysis and membrane isolation, and crossed immunoelectrophoresis. Section E describes tests designed to detect specific enzymatic activities or nutritional requirements of mycoplasmas. These include sugar fermentation and arginine hydrolysis tests as well as tests to establish the sterol requirement of mycoplasmas. Section F deals with serological tests. These tests either have specific application to detection of mycoplasma antigens or antibody in plant and animal tissues; are useful in identification of specific groups of mycoplasmas (spiroplasmas); or provide higher sensitivity in measurements of antibodies against mycoplasmas.
Author:
Publisher: Academic Press
ISBN: 0080859550
Category : Science
Languages : en
Pages : 389
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Book Description
This volume provides complete and thorough coverage of the classical and state-of-the-art methods used in cell culture. It also includes basic principles used in the selection of cells for specific scientific study, as well as analytical and procedural techniques.Key Features* Reviews basic principles of cell culture* Gives options and techniques on how to look at cells
Author: Ivano de Filippis
Publisher: Springer Science & Business Media
ISBN: 1627031855
Category : Science
Languages : en
Pages : 475
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Book Description
Molecular Typing in Bacterial Infections covers common bacterial pathogenic agents, with the most effective methods for their identification and classification in the light of their specific epidemiology. The book will be a valuable resource for molecular typing of infectious diseases agents encountered in both the research and hospital clinical lab settings, as well as culture collections. Each chapter provides an overview of molecular approaches to typing bacterial pathogens. Part I gives a general overview of typing methods used in the traditional microbiology laboratory in comparison to molecular methods of epidemiology. In Part II, the relative strengths and weaknesses of the different methods applicable to the specific agents of infectious diseases are emphasized. Specific emphasis is placed on recent changes and updates in molecular typing.
Author: Patricia Hernandez-Rodriguez
Publisher: BoD – Books on Demand
ISBN: 9535106120
Category : Science
Languages : en
Pages : 582
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Book Description
This book is intended to present current concepts in molecular biology with the emphasis on the application to animal, plant and human pathology, in various aspects such as etiology, diagnosis, prognosis, treatment and prevention of diseases as well as the use of these methodologies in understanding the pathophysiology of various diseases that affect living beings.
Author: Wayne P. Olson
Publisher: CRC Press
ISBN: 9780935184822
Category : Medical
Languages : en
Pages : 418
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Book Description
This book focuses on practical, proven applications to automate the microbial identification process economically and with greater levels of safety and quality for patients. A diverse group of recognized experts survey the topic and present the latest techniques and technologies for microbial detection. They cover bacteria and yeasts, the technology of automation, equipment, methods, and the validation issues involved in "going automated." They also explore the challenges of detection and quantititation of contaminants in the increasing number of biologic injectable drugs and identify current trends in the industry. Features
Author: Ivano de Filippis
Publisher: Springer Nature
ISBN: 3030740188
Category : Science
Languages : en
Pages : 222
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Book Description
This updated second edition of Molecular Typing in Bacterial Infections, presented in two volumes, covers both common and neglected bacterial pathogenic agents, highlighting the most effective methods for their identification and classification in the light of their specific epidemiology. New chapters have been included to add new species, as well as another view of how bacterial typing can be used. These books are valuable resources for the molecular typing of infectious disease agents encountered in both research and hospital clinical laboratory settings, as well as in culture collections and in the industry. Each of the 21 chapters provides an overview of specific molecular approaches to efficiently detect and type different bacterial pathogens. The chapters are grouped in five parts, covering respiratory and urogenital pathogens (Volume I), and gastrointestinal and healthcare-associated pathogens, as well as a new group of vector-borne and Biosafety level 3 pathogens including a description of typing methods used in the traditional microbiology laboratory in comparison to molecular methods of epidemiology (Volume II). Comprehensive and updated, Molecular Typing in Bacterial Infections provides state-of-the-art methods for accurate diagnosis and for the correct classification of different types which will prove to be critical in unravelling the transmission routes of human pathogens.
