Author:
Publisher:
ISBN:
Category :
Languages : en
Pages : 8
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Book Description
The objective of this work is to develop a novel sensing platform for the direct and rapid detection of biological agents. To achieve this goal, we are building on our portable DNA Biological Detection System work by incorporating a new class of molecular recognition elements, known as aptamers. In this paper, the nucleic acid-based sensor work conducted in our laboratory is discussed including initial work on the design and optimization of aptamer biorecognition sensor elements targeting a relevant biological agent surrogate (i.e., Bacillus globigii) and the development of a portable nucleic acid detection system.
Author: Günter Mayer
Publisher: Springer Nature
ISBN: 1071626957
Category : Science
Languages : en
Pages : 283
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Book Description
This updated book reflects improvements in a variety of techniques used to study the aptamer field. Beginning with a section on selection procedures, the volume continues with methods to characterize aptamers’ interaction and structural properties by biophysical approaches, as well as a variety of applications that have been adapted to the aptamer compound class. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Nucleic Acid Aptamers: Selection, Characterization, and Application, Second Edition serves as an ideal guide for researchers aiming to further our understanding of aptamer biology and more.
Author: Günter Mayer
Publisher: Humana
ISBN: 9781493931965
Category : Science
Languages : en
Pages : 0
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Book Description
This volume provides protocol references covering recent developments in the aptamer field. Within the last decade, aptamers have become more and more popular, and their sophisticated biophysical properties together with their ability to be easily modified and, thus, adapted to various regimens makes them a very promising class of compounds. Divided into three sections, the book covers selection, a series of analytical methods to assess biophysical properties of aptamer-target interactions, as well as various applications of aptamers. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and easy to follow, Nucleic Acid Aptamers: Selection, Characterization, and Application provides a state-of-the-art summary of recent developments in the aptamer field and will be a helpful resource for scientists in the life sciences working with aptamers as tools to elucidate biological systems.
Author: Katharina Urmann
Publisher: Springer Nature
ISBN: 3030540618
Category : Science
Languages : en
Pages : 217
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Book Description
This book reviews the development, characterization and applications of aptamers in different areas of biotechnology ranging from therapeutics to diagnostics and protein purification. Hailed as chemical antibodies, these single-stranded nucleic acid receptors were predicted to supersede antibodies in traditional assays, such as ELISA, within a short time. While this has yet to happen, readers will find in this book a deep insight into the progress of aptamer technology and a critical discussion about the limitations that need to be overcome in order to find wider acceptance and use outside of the still relatively small aptamer-community. This book covers all aspects of aptamer generation and application for the aptamer-experienced reader and curious novice alike, with the addition of an industry perspective on the future of aptamer-use in biotechnology.
Author: Nawel Mejri
Publisher:
ISBN:
Category :
Languages : en
Pages : 0
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Book Description
This aim of this work is to develop ultrasensitive electrochemical biosensors with high affinity toward ochratoxine (OTA) and aflatoxine M1 (AFM1). In order to obtain the best analytical performances, we associated nano-materials in the transducer construction: conducting polypyrrole polymer and poly(amido-amine) dendrimères. Thanks to this association, we benefited from the conducting material's electrical properties, and the large active detection surface dendrimers. For the bimolecular sensing part, we used specific DNA aptamers which allowed us to quantify mycotoxines at nM concentrations. In addition, the different aptamer based biosensors present a very large dynamic ranges. We also demonstrated through the use of different sizes of dendrimers, that the sensitivity depend not only in the affinity between bioreceptors and their target molecules, but also in the physico-chemical properties of the biosensor.
Author: Arpana Parihar
Publisher: CRC Press
ISBN: 1003829503
Category : Technology & Engineering
Languages : en
Pages : 375
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Book Description
The design and fabrication of aptamer-based biosensors for point-of-care testing of disease diagnostic and prognostic is an upthrust and interdisciplinary area of research. This book covers the design and development of novel cost-effective aptamer-based biosensors for disease diagnostic and prognostic including an understanding of health care management in terms of timely updates of disease situations in a particular geographical area. It also discusses the scaling up and market trend of aptamer-based devices for extension of research from lab to market, and end users. Features: Explains the design and fabrication of aptasensors-based diagnostic devices. Includes novel approaches and comprehensive technology overview for diagnosis management towards early-stage detection of various biomarkers associated with several health conditions. Demonstrates possible benefits of combined diagnostics and therapeutic possibilities using aptamer-based point-of-care technologies devices. Discusses emerging implications and recent advances of biosensing platforms for designing and developing aptamer-based point-of-care testing (POCT) devices. Explores aptamer-based approach to develop advanced platforms for medical applications and open arena for state-of-the-art future research. This book is aimed at researchers and graduate students in biomedical engineering, diagnostics, nanobiotechnology, and materials science.
Author: Gulab Singh Yadav
Publisher: Springer Nature
ISBN: 9811388369
Category : Science
Languages : en
Pages : 186
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Book Description
The book discusses the basics of aptamers and the advent of aptamer-based technology in recent times. The book covers the diverse applications of aptamers, such as in detection of animal and plant pathogens, disease diagnosis and therapeutics, environmental contamination detection etc. Besides these applications, the book also describes the use of these synthetic or modified DNA, as drug delivery vehicles. The different chapters describe how the binding capacity and specificity of aptamers can be exploited in various ways. The book also discusses how these attributes of aptamers can outdo the antibody technology in biomedical and diagnostic solutions. This crisp and concise book gives the readers an insight into the most recent biotechnological applications of aptamers.
Author: Sven Klussmann
Publisher: John Wiley & Sons
ISBN: 3527607919
Category : Science
Languages : en
Pages : 518
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Book Description
In The Aptamer Handbook, leading scientists from academia as well as biotech and pharma companies introduce the revolutionary concept of designing RNA and DNA oligonucleotides with novel functions by in vitro selection. These functions comprise high affinity binding (aptamers), catalytic activity (ribozymes and deoxyribozymes) or combinations of binding and catalytic properties (aptazymes). Basic concepts and technologies describing in detail how these functional oligonucleotides can be identified are presented. Numerous examples demonstrate the versatility of in vitro selected oligonucleotides. Special emphasis has been put on a section that shows the broad applicability of aptamers, e. g. in target validation, for analytics, or as new therapeutics. This first overview in the field is of prime interest for a broad audience of scientists both in academia and in industry who wish to expand their knowledge on the potential of new oligonucleotide functions and their applications.
Author: Man Bock Gu
Publisher: Springer
ISBN: 3642541437
Category : Technology & Engineering
Languages : en
Pages : 338
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Book Description
Volumes are organized topically and provide a comprehensive discussion of developments in the respective field over the past 3-5 years. The series also discusses new discoveries and applications. Special volumes are dedicated to selected topics which focus on new biotechnological products and new processes for their synthesis and purification. In general, special volumes are edited by well-known guest editors. The series editor and publisher will however always be pleased to receive suggestions and supplementary information. Manuscripts are accepted in English.
Author: Yiyang Dong
Publisher: Springer Nature
ISBN: 9813348380
Category : Science
Languages : en
Pages : 470
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Book Description
This book outlines comprehensively the main medical uses of aptamers, from diagnosis to therapeutics in fourteen chapters. Pioneering topics covered include aptamer pharmaceuticals, aptamers for malign tumors, aptamers for personalized therapeutics and aptamers for point-of-care testing. The book offers an essential guide for medical scientists interested in developing aptamer-based schemes for better theranostics. It is therefore of interest for not only academic researchers, but also practitioners and medical researchers in various fields of medical science, medical research and bio-analytical chemistry.
