Characterisation of Macromolecules Using Electrospray Ionization and Fourier Transform Ion Cyclotron Resonance Mass Spectrometry PDF Download
Are you looking for read ebook online? Search for your book and save it on your Kindle device, PC, phones or tablets. Download Characterisation of Macromolecules Using Electrospray Ionization and Fourier Transform Ion Cyclotron Resonance Mass Spectrometry PDF full book. Access full book title Characterisation of Macromolecules Using Electrospray Ionization and Fourier Transform Ion Cyclotron Resonance Mass Spectrometry by Liam Andrew McDonnell. Download full books in PDF and EPUB format.
Author: Liam Andrew McDonnell
Publisher:
ISBN:
Category :
Languages : en
Pages : 0
Get Book Here
Book Description
Author: Liam Andrew McDonnell
Publisher:
ISBN:
Category :
Languages : en
Pages : 0
Get Book Here
Book Description
Author:
Publisher:
ISBN:
Category :
Languages : en
Pages :
Get Book Here
Book Description
The field of mass spectrometry has grown tremendously over the past few decades due in large part to the continued application to new and interesting areas of exploration. The advent of soft ionization sources such as electrospray ionization and matrix-assisted laser desorption/ionization have dramatically increased the capabilities of mass spectrometry and increased the amount of information derived from biological samples. As a result, there has been tremendous growth in the development of new ionization source technology in recent years. One of the main driving forces behind the development of new ionization technology is for the reduction of sample preparation required prior to analysis, yielding high throughput sample analysis. Demonstrated herein is the development and characterization of an atmospheric pressure ionization source called matrix-assisted laser desorption electrospray ionization (MALDESI). MALDESI is a hybrid combination of MALDI and ESI which utilizes laser desorption with electrospray postionization for the generation of multiply-charged ions. Multiply-charged ions are of particular importance when using Fourier transform mass spectrometry, due to the increase in resolving power and mass accuracy with increasing charge on the molecule. Top-down characterization of intact polypeptides is demonstrated as well as high mass accuracy utilizing internal calibration. A newly designed highly robust and versatile atmospheric pressure ionization platform is developed and described in detail. Solid- and liquid state analysis of three out of the four classes of biological molecules is demonstrated utilizing the newly developed versatile ionization platform. MALDESI utilizing ultraviolet and infrared laser desorption is demonstrated at various wavelengths (UV, 337 nm and 349 nm and IR, 2.94 Ã'Âæm and 10.6 Ã'Âæm) with and without ESI postionization for the generation of multiply-charged ions. The characteristics of liquid-state samples are described as a mac.
Author: Haizhen Zhang
Publisher:
ISBN: 9781109852523
Category : Electronic dissertations
Languages : en
Pages : 136
Get Book Here
Book Description
Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) is an ideal technique for investigation of host-guest supramolecular complexes due to its ultra-high mass resolving power, ultra-high mass accuracy, and high sensitivity. Moreover it has the capability of trapping ions for ion chemistry, and versatile tandem mass spectrometry capabilities.
Author: Birendra N. Pramanik
Publisher: CRC Press
ISBN: 1135560439
Category : Science
Languages : en
Pages : 478
Get Book Here
Book Description
Discussing strategies to determine the structure and machanisms of numerous compound classics, this book covers new chemical and elctrophoretic techniques for rapid sample preconcentration and separation. It summarizes breakthroughs in the theory and instrumentation of electrospray mass spectrometry in pharmaceutical and biomedical applications, provides practical examples for the characterization of peptides, proteins, and glycoproteins, includes applications in proteomics, combinatorial chemistry, and drug characterization. Topics include chemical and electrophoretic techniques for rapid sample preconcentration and separation, screening processes for proteins from libraries of compounds, protein folding and dynamics, and more.
Author: Jason Scott Sampson
Publisher:
ISBN:
Category :
Languages : en
Pages : 136
Get Book Here
Book Description
Keywords: FT-ICR, hybrid ionization, electrospray, matrix-assisted laser desorption electrospray ion, MALDESI.
Author: Huan He
Publisher:
ISBN:
Category :
Languages : en
Pages : 121
Get Book Here
Book Description
ABSTRACT: Chapter 2 describes various aspects of a developed method for polar lipid semi-quantification. Simple liquid-liquid extraction and one-step nano-LC separation are important for analyses of complex cellular lipid mixtures -- We observed more than 20 fold increases in detected lipid species after nano-LC separation. This method was applied on glioma cell lines, U87, under different combination of gene-/chemotherapy. Combination of adenovirus wt-p53 transfection and topoisomerase inhibition results in cells' G2 arrest and apoptosis. A sugar binding protein, galectin-1, was down-regulated under such treatment. Lipidomics study identified the associated changes in the polar lipidome - the decrease of short chain asialo-GM1 (a ligand of galectin-1), decreases of long chain gangliosides, increases of short chain sulfatides and increases of all phospholipids, especially hydroxylated phospholipids.
Author: Ying Xiong
Publisher:
ISBN:
Category : Biomolecules
Languages : en
Pages :
Get Book Here
Book Description
Author: Richard B. Cole
Publisher: John Wiley & Sons
ISBN: 1118211553
Category : Science
Languages : en
Pages : 900
Get Book Here
Book Description
Discover how advances in mass spectrometry are fueling new discoveries across a broad range of research areas Electrospray and MALDI Mass Spectrometry brings both veteran practitioners and beginning scientists up to date with the most recent trends and findings in electrospray ionization and matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. In particular, this Second Edition highlights how advances in electrospray and MALDI mass spectrometry are supporting important discoveries in new and emerging fields such as proteomics and metabolomics as well as in traditional areas of chemistry and physics research. Electrospray AND MALDI Mass Spectrometry, SECOND EDITION is divided into five parts: Part A, Fundamentals of ES, explains the fundamental phenomena underlying the electrospray process, including selectivity in ionization and inherent electrochemistry, and concludes with a chapter offering a comparative inventory of source hardware Part B, Fundamentals of MALDI, confronts ionization mechanisms, instrument development, and matrix selection, and includes a final chapter that explores the special application of MALDI to obtain two-dimensional images of spatial distributions of compounds on surfaces Part C, ES and MALDI Coupling to Mass Spectrometry Instrumentation, examines the coupling of these ionization techniques to various mass analyzers, including quadrupole ion trap, time-of-flight, Fourier transform ion cyclotron resonance, and ion mobility mass spectrometers Part D, Practical Aspects of ES and MALDI, investigates analytical issues including quantification, charge-state distributions, noncovalent interactions in solution that are preserved as gas-phase ions, and various means of ion excitation in preparation for tandem mass spectrometry, and offers a guide to the interpretation of even-electron mass spectra Part E, Biological Applications of ES and MALDI, examines the role of mass spectrometry in such areas as peptide and protein characterization, carbohydrate analysis, lipid analysis, and drug discovery Written by a team of leading experts, the book not only provides a critical review of the literature, but also presents key concepts in tutorial fashion to help readers take full advantage of the latest technological breakthroughs and applications. As a result, Electrospray and MALDI Mass Spectrometry will help researchers fully leverage the power of electrospray and MALDI mass spectrometry. The judicious compartmentalization of chapters, and the pedagogic presentation style throughout, render the book highly suitable for use as a text for graduate-level courses in advanced mass spectrometry.
Author: Magnus Palmblad
Publisher: Uppsala Universitet
ISBN: 9789155452964
Category : Medical
Languages : en
Pages : 76
Get Book Here
Book Description
Author: Robert Edward Bossio
Publisher:
ISBN:
Category : Electrospray ionization mass spectrometry
Languages : en
Pages : 444
Get Book Here
Book Description
