Applications of Tracer Kinetic Techniques to the Study of Fuel Metabolism in Vivo PDF Download
Are you looking for read ebook online? Search for your book and save it on your Kindle device, PC, phones or tablets. Download Applications of Tracer Kinetic Techniques to the Study of Fuel Metabolism in Vivo PDF full book. Access full book title Applications of Tracer Kinetic Techniques to the Study of Fuel Metabolism in Vivo by Arthur Brian Jenkins. Download full books in PDF and EPUB format.
Author: Arthur Brian Jenkins
Publisher:
ISBN:
Category : Glucose
Languages : en
Pages : 500
Get Book Here
Book Description
Author: Arthur Brian Jenkins
Publisher:
ISBN:
Category : Glucose
Languages : en
Pages : 500
Get Book Here
Book Description
Author: Reginald Shipley
Publisher: Elsevier
ISBN: 0323158900
Category : Science
Languages : en
Pages : 254
Get Book Here
Book Description
Tracer Methods for In Vivo Kinetics: Theory and Applications summarizes and evaluates the variety of working formulas in applying tracer methods for kinetic studies in vivo. This book discusses the basic concepts, derives the important equations, and evaluates each working formula to ensure its proper application within the set of experimental conditions available. Organized into 12 chapters, this book starts with an overview of tracers such as radioactive atoms. This text then discusses the errors inherent in treating a two-pool system as though it were one pool. Other chapters describe how, from curves of tracer content of the labeled pool or of the secondary pool, a definitive evaluation is made of rate constants and rate of interflow and outflow in the two-pool open system. This book discusses as well the limited usefulness of a curve of specific gravity when information is limited to such a curve obtained from the secondary pool. This book is intended for mathematicians, biomedical scientists, and graduate students.
Author: Randall Arnold Hawkins
Publisher:
ISBN:
Category : Fluorine
Languages : en
Pages : 256
Get Book Here
Book Description
Author: Kerry A. Riffel
Publisher:
ISBN: 9781109865639
Category :
Languages : en
Pages : 94
Get Book Here
Book Description
Clinical trials routinely include the analysis of plasma samples to determine pharmacokinetic and metabolic properties of new drug entities. However, the determination of drug concentration in the brain is difficult and usually inferred from terminal studies in other species or from sampling of cerebrospinal fluid (CSF). Positron emission tomography (PET) is a minimally invasive imaging procedure widely used for the evaluation of cancer patients. In recent years, PET has gained acceptance as a research tool in drug development. Positron emitting isotopes are used in PET to quantitate regional tissue distribution of radioactivity in living animals and humans. Accurate use of PET requires assessment of metabolism of the tracer to avoid erroneous results from the data. Metabolism of PET tracers can be evaluated in vivo in animal studies and in human subjects by sampling plasma. In vitro metabolism can be used to evaluate and compare metabolism of novel PET tracers in different species. In addition, significant plasma protein binding can prevent a tracer from reaching the target of interest. Thus, PET tracers for the metabotropic glutamate subtype 5 receptor (mGluR5), angiotensin receptor (AT1), and neurokinin-1 (NK1) receptor were chosen to conduct method development and optimization of analytical techniques to rapidly analyze radiolabeled metabolites from in vivo plasma samples collected during animal imaging studies and in vitro metabolism studies. Plasma protein binding methods were also optimized for the same tracers. Optimized methods included the use of a monolithic column for HPLC analysis, resulting in significantly reduced sample analysis times. Plasma protein binding measurements were optimized for the use of centrifuge filters by pre-treating the filters with unlabeled tracer to prevent nonspecific binding of the tracer to the filters. These procedures were used retrospectively to determine whether the studies would have aided the selection of a tracer for clinical studies and/or improved the interpretation of imaging studies. Finally, the methods were applied to two tracers currently in development. The methods and procedures were successful in providing additional support to the evaluation of two novel tracers and are currently used routinely to support tracer development in Imaging Research at Merck Research Laboratories.
Author:
Publisher:
ISBN:
Category : Nuclear energy
Languages : en
Pages : 612
Get Book Here
Book Description
Author:
Publisher:
ISBN:
Category : Dissertations, Academic
Languages : en
Pages : 924
Get Book Here
Book Description
Author: Robert R. Wolfe
Publisher: John Wiley & Sons
ISBN: 9780471462095
Category : Medical
Languages : en
Pages : 494
Get Book Here
Book Description
In the past few years, the number of applications of tracers for in vivo biomedical studies has greatly increased. New analytical tools at the genetic and protein levels have spurred this growth, opening the door for a deeper understanding of metabolic events. This in turn promises to yield significant advances in the understanding and treatment of human disease. Now fully revised and expanded, Isotope Tracers in Metabolic Research, Second Edition is the established definitive text on stable and radioactive isotope tracers. In unique, multidisciplinary fashion, it presents comprehensive coverage of new methodological, mathematical, and theoretical approaches. This new Second Edition includes: All-new chapters on nuclear magnetic resonance, mass isotopomer analysis, and methods of protein metabolism analysis A completely updated categorized list of over 750 references Major advances in the development of mass isotopomer and positional isotopomer techniques, noninvasive isotope techniques for studying metabolic pathways, hyphenated techniques, and new tracer techniques The latest developments in quantification of DNA synthesis and mass spectrometry spurred by genome sequencing and proteomics New coverage of mathematical modeling Expanded coverage of microdialysis probes, laboratory procedures, and regulatory issues related to human studies In this complete guide to performing tracer studies, the authors systematically cover tracer selection, modeling considerations, sample derivitization, mass spectrometry analysis, and data interpretation. Problems and discussion questions highlight key points in each chapter. Isotope Tracers in Metabolic Research, Second Edition offers students and researchers a comprehensive, practical resource for utilizing the latest tracer methodologies.
Author: Norbert W. Lutz
Publisher: Cambridge University Press
ISBN: 0521765900
Category : Medical
Languages : en
Pages : 641
Get Book Here
Book Description
Metabolomics, the global characterisation of the small molecule complement involved in metabolism, has evolved into a powerful suite of approaches for understanding the global physiological and pathological processes occurring in biological organisms. The diversity of metabolites, the wide range of metabolic pathways and their divergent biological contexts require a range of methodological strategies and techniques. Methodologies for Metabolomics provides a comprehensive description of the newest methodological approaches in metabolomic research. The most important technologies used to identify and quantify metabolites, including nuclear magnetic resonance and mass spectrometry, are highlighted. The integration of these techniques with classical biological methods is also addressed. Furthermore, the book presents statistical and chemometric methods for evaluation of the resultant data. The broad spectrum of topics includes a vast variety of organisms, samples and diseases, ranging from in vivo metabolomics in humans and animals to in vitro analysis of tissue samples, cultured cells and biofluids.
Author: Alan R. Saltiel
Publisher: Springer Science & Business Media
ISBN: 0387722041
Category : Medical
Languages : en
Pages : 223
Get Book Here
Book Description
More than 18 million people in the United States have diabetes mellitus, and about 90% of these have the type 2 form of the disease. This book attempts to dissect the complexity of the molecular mechanisms of insulin action with a special emphasis on those features of the system that are subject to alteration in type 2 diabetes and other insulin resistant states. It explores insulin action at the most basic levels, through complex systems.
Author: United States. Energy Research and Development Administration
Publisher:
ISBN:
Category : Medicine
Languages : en
Pages :
Get Book Here
Book Description
