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Author: Reece Kimball Hart
Publisher:
ISBN:
Category :
Languages : en
Pages : 182
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Author: Reece Kimball Hart
Publisher:
ISBN:
Category :
Languages : en
Pages : 182
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Author: Michael C. Giddings
Publisher:
ISBN:
Category :
Languages : en
Pages : 434
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Author: Mark D. Adams
Publisher: Elsevier
ISBN: 0080926398
Category : Science
Languages : en
Pages : 389
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Book Description
A timely book for DNA researchers, Automated DNA Sequencing and Analysis reviews and assesses the state of the art of automated DNA sequence analysis-from the construction of clone libraries to the developmentof laboratory and community databases. It presents the methodologies and strategies of automated DNA sequence analysis in a way that allows them to be compared and contrasted. By taking a broad view of the process of automated sequence analysis, the present volume bridges the gap between the protocols supplied with instrument and reaction kits and the finalized data presented in the research literature. It will be an invaluable aid to both small laboratories that are interested in taking maximum advantageof automated sequence resources and to groups pursuing large-scale cDNA and genomic sequencing projects. The field of automation in DAN sequencing and analysis is rapidly moving, this book fulfils those needs, reviews the history of the art and provides pointers to future development.
Author: Murugathas Yuwaraj
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Book Description
Electrophoresis-based automated identification of the Deoxyribo Nucleic Acid (DNA) sequence provides a means of detecting variations in the DNA of an organism at the nucleotide level. Accurate detection of these variations and the DNA sequence, in general, are limited by statistical variations in the DNA time-series and overlap of adjacent peaks due to limited resolution. In this thesis, a local maximum likelihood processor (L-DNA), which is based on a statistical model of the DNA time-series is developed. The L-DNA poor generates a finite set of hypothesized sequences. For each hypothesized sequence, model parameters estimated from the observed waveform are used to evaluate the likelihood of the hypothesized sequence being consistent with the observed waveform. The hypothesis with the highest likelihood is selected as the local sequence in the observed region. The performance of the L-DNA processor is evaluated using both simulated and real DNA data. Simulations show that in low resolution regions of the DNA data, which are observed towards the end of a sequencing run, the probability of error for the proposed processor is two orders of magnitude smaller than the commonly used Jansson-van Cittert iterative deconvolution method. The robustness of the processor is illustrated by sequentially extending the read length of a fragment of DNA sequence. The ability of the processor to detect heterozygous sites and partial mutation sites in low resolution regions is illustrated using 30 segments of DNA data where the proportion of the wild and mutant types is artificially controlled. The L-DNA processor correctly identifies 80% of the data, while a commercial base-calling program correctly detects only 33% of the data. It should be noted that the commercial base-calling program is specifically designed for the sequencer that was used to acquire the above mentioned data. The L-DNA processor provides a measure of confidence for each hypothesized sequence. This measure can be used to significantly reduce the amount of human intervention needed to edit results of automated DNA sequencing system in clinical applications.
Author:
Publisher:
ISBN:
Category :
Languages : en
Pages : 11
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An object model for genome data at all levels of resolution is described. The model was derived by considering the requirements for representing genome related objects in three application domains: genome maps, large-scale DNA sequencing, and exploring functional information in gene and protein sequences. The methodology used for the object-oriented analysis is also described.
Author:
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Author: Wade H. Shafer
Publisher: Springer Science & Business Media
ISBN: 1461303931
Category : Science
Languages : en
Pages : 427
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Book Description
Masters Theses in the Pure and Applied Sciences was first conceived, published, and disseminated by the Center for Information and Numerical Data Analysis and Synthesis (CINDAS)* at Purdue University in 1957, starting its coverage of theses with the academic year 1955. Beginning with Volume 13, the printing and dis semination phases of the activity were transferred to University Microfilms/Xerox of Ann Arbor, Michigan, with the thought that such an arrangement would be more beneficial to the academic and general scientific and technical community. After five years of this joint undertaking we had concluded that it was in the interest of all concerned if the printing and distribution of the volumes were handled by an international publishing house to assure improved service and broader dissemination. Hence, starting with Volume 18, Masters Theses in the Pure and Applied Sciences has been disseminated on a worldwide basis by Plenum Publishing Corporation of New York, and in the same year the coverage was broadened to include Canadian universities. All back issues can also be ordered from Plenum. We have reported in Volume 39 (thesis year 1994) a total of 13,953 thesis titles from 21 Canadian and 159 United States universities. We are sure that this broader base for these titles reported will greatly enhance the value of this impor tant annual reference work. While Volume 39 reports theses submitted in 1994, on occasion, certain uni versities do report theses submitted in previous years but not reported at the time.
Author: Christoph W. Sensen
Publisher: John Wiley & Sons
ISBN: 3527612653
Category : Science
Languages : en
Pages : 442
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Book Description
Provides an overview of the rapidly evolving field of genomics with coverage of nucleic acid technologies, proteomics and bioinformatics. It includes chapters on applications in human health, agriculture and comparative genomics and also contains two chapters on the legal and ethical issues of genomics, a topic that is becoming increasingly important as genomics moves out of the laboratory into practical applications.
Author:
Publisher:
ISBN:
Category : Medicine
Languages : en
Pages : 866
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Author:
Publisher:
ISBN:
Category : Medicine
Languages : en
Pages : 1808
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